| YAL067W-A |
YAL067W-A |
Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; Belongs to the UPF0377 family |
| SEO1 |
YAL067C |
Probable transporter SEO1; Putative permease; member of the antoate transporter subfamily of the major facilitator superfamily; mutation confers resistance to ethionine sulfoxide |
| YAL065C |
YAL065C |
Uncharacterized protein YAL065C; Putative protein of unknown function; shows sequence similarity to FLO1 and other flocculins |
| YAL064W-B |
YAL064W-B |
Uncharacterized membrane protein YAL064W-B; Fungal-specific protein of unknown function |
| GDH3 |
YAL062W |
NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources; GDH3 has a paralog, GDH1, that arose from the whole genome duplication; Belongs to the Glu/Leu/Phe/Val dehydrogenases family |
| BDH2 |
YAL061W |
Probable diacetyl reductase [(R)-acetoin forming] 2; Putative medium-chain alcohol dehydrogenase with similarity to BDH1; transcription induced by constitutively active PDR1 and PDR3; Belongs to the zinc-containing alcohol dehydrogenase family |
| BDH1 |
YAL060W |
NAD-dependent (R,R)-butanediol dehydrogenase; catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source |
| ECM1 |
YAL059W |
Shuttling pre-60S factor ECM1; Pre-ribosomal factor involved in 60S ribosomal protein subunit export; associates with the pre-60S particle; shuttles between the nucleus and cytoplasm; Belongs to the ECM1 family |
| CNE1 |
YAL058W |
Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast |
| GPB2 |
YAL056W |
Guanine nucleotide-binding protein subunit beta 2; Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; inhibits Ras activity through direct interactions with Ira1p/2p; regulated by G-alpha protein Gpa2p; GPB2 has a paralog, GPB1, that arose from the whole genome duplication |
| PEX22 |
YAL055W |
Peroxisome assembly protein 22; Putative peroxisomal membrane protein; required for import of peroxisomal proteins; functiony complements a Pichia pastoris pex22 mutation; Belongs to the peroxin-22 family |
| ACS1 |
YAL054C |
Acetyl-coA synthetase isoform; along with Acs2p, acetyl-coA synthetase isoform is the nuclear source of acetyl-coA for histone acetylation; expressed during growth on nonfermentable carbon sources and under aerobic conditions; Belongs to the ATP-dependent AMP-binding enzyme family |
| FLC2 |
YAL053W |
Flavin carrier protein 2; Putative calcium channel involved in calcium release under hypotonic stress; required for uptake of FAD into endoplasmic reticulum; involved in cell w maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication |
| OAF1 |
YAL051W |
Oleate-activated transcription factor; subunit of a heterodimeric complex with Pip2p, which binds to oleate-response elements (ORE) in the promoter of genes involved in beta-oxidation of fatty acids, peroxisome organization and biogenesis, activating transcription in the presence of oleate; regulates chromatin silencing at telomeres; involved in diauxic shift; OAF1 has a paralog, PIP2, that arose from the whole genome duplication |
| AIM2 |
YAL049C |
Cytoplasmic protein involved in mitochondrial function or organization; null mutant displays reduced frequency of mitochondrial genome loss; potential Hsp82p interactor; Belongs to the AIM2 family |
| GEM1 |
YAL048C |
Outer mitochondrial membrane GTPase, subunit of the ERMES complex; potential regulatory subunit of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth; cells lacking Gem1p contain collapsed, globular, or grape-like mitochondria; ortholog of metazoan Miro GTPases |
| SPC72 |
YAL047C |
Spindle pole component SPC72; Gamma-tubulin sm complex (gamma-TuSC) receptor; recruits the gamma-TuSC complex to the cytoplasmic side of the SPB, connecting nuclear microtubules to the SPB; involved in astral microtubule formation, stabilization, and with Stu2p, anchoring astral MTs at the cytoplasmic face of the SPB, and regulating plus-end MT dynamics; regulated by Cdc5 kinase |
| BOL3 |
YAL046C |
BolA-like protein 3; Protein involved in Fe-S cluster transfer to mitochondrial clients; protects [4Fe-4S] clusters from damage due to oxidative stress by acting along with Nfu1p at a late step in the transfer of [4Fe-4S] clusters from the ISA complex to mitochondrial client proteins like lipoate synthase and succinate dehydrogenase; sequence similarity to human BOLA family member, BOLA3, mutations of which are associated with Multiple Mitochondria Dysfunctions Syndrome (MMDS2) |
| BOL1 |
YAL044W-A |
BolA-like protein 1; Mitochondrial matrix protein involved in Fe-S cluster biogenesis; facilitates [4Fe-2S] cluster inception into mitochondrial proteins such as lipoate synthase and succinate dehydrogenase; interacts and may function with Grx5p at an early step in Fe-S cluster biosynthesis; forms dimeric complexes with Grx5p and Nfu1p that alter the stability of shared Fe/S clusters; sequence similarity to human BOLA family member, BOLA1 and S. pombe uvi31, a putative DNA repair protein |
| GCV3 |
YAL044C |
H subunit of the mitochondrial glycine decarboxylase complex; glycine decarboxylase is required for the catabolism of glycine to 5,10-methylene-THF; also required for protein lipoylation; expression is regulated by levels of 5,10-methylene-THF; Belongs to the GcvH family |
| PTA1 |
YAL043C |
Pre-tRNA-processing protein PTA1; Subunit of holo-CPF; holo-CPF is a multiprotein complex and functional homolog of mammalian CPSF, required for the cleavage and polyadenylation of mRNA and snoRNA 3' ends; involved in pre-tRNA processing; binds to the phosphorylated CTD of RNAPII |
| ERV46 |
YAL042W |
Endoplasmic reticulum-golgi intermediate compartment protein 3; Protein localized to COPII-coated vesicles; forms a complex with Erv41p; involved in the membrane fusion stage of transport; Belongs to the ERGIC family |
| CDC24 |
YAL041W |
Cell division control protein 24; Guanine nucleotide exchange factor (GEF) for Cdc42p; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress; thermosensitivity of the cdc24-4 mutant in the presence of sorbitol is functiony complemented by human CDC42 |
| CLN3 |
YAL040C |
G1/S-specific cyclin CLN3; G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote G1 to S phase transition; plays a role in regulating transcription of other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis; acetyl-CoA induces CLN3 transcription in response to nutrient repletion to promote cell-cycle entry; cell cycle arrest phenotype of the cln1 cln2 cln3 triple null mutant is complemented by any of human cyclins CCNA2, CCNB1, CCNC, CCND1, or CCNE1 |
| CYC3 |
YAL039C |
Cytochrome c heme lyase (holocytochrome c synthase); attaches heme to apo-cytochrome c (Cyc1p or Cyc7p) in mitochondrial intermembrane space; human homolog HCCS implicated in microphthalmia with linear skin defects (MLS), and can complement yeast null mutant |
| CDC19 |
YAL038W |
Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via osteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication |
| YAL037C-A |
YAL037C-A |
Putative uncharacterized protein yal037c-a; Putative protein of unknown function |
| YAL037W |
YAL037W |
Uncharacterized protein yal037w; Putative protein of unknown function; YAL037W has a paralog, YOR342C, that arose from the whole genome duplication |
| RBG1 |
YAL036C |
Ribosome-interacting GTPase 1; Member of the DRG family of GTP-binding proteins; associates with translating ribosomes; interacts with Tma46p, Ygr250cp, Gir2p and Yap1p via two-hybrid; Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family |
| FUN12 |
YAL035W |
Translation initiation factor eIF5B; GTPase that promotes Met-tRNAiMet binding to ribosomes and ribosomal subunit joining; promotes GTP-dependent maturation of 18S rRNA by Nob1p; protein abundance increases in response to DNA replication stress; homolog of bacterial IF2 |
| MTW1 |
YAL034W-A |
Kinetochore-associated protein MTW1; Essential component of the MIND kinetochore complex; joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly; complex consists of Mtw1p Including Nnf1p-Nsl1p-Dsn1p (MIND) |
| FUN19 |
YAL034C |
SWIRM domain-containing protein FUN19; Non-essential protein of unknown function; expression induced in response to heat stress; FUN19 has a paralog, YOR338W, that arose from the whole genome duplication |
| POP5 |
YAL033W |
Ribonuclease P/MRP protein subunit POP5; Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs |
| PRP45 |
YAL032C |
Snw domain-containing protein 1; Pre-mRNA-processing protein 45; Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene |
| GIP4 |
YAL031C |
GLC7-interacting protein 4; Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; protein overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; potential Cdc28p substrate |
| SNC1 |
YAL030W |
Synaptobrevin homolog 1; Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; proposed to be involved in endocytosis; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC1 has a paralog, SNC2, that arose from the whole genome duplication |
| MYO4 |
YAL029C |
Myosin-4; Type V myosin motor involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p; MYO4 has a paralog, MYO2, that arose from the whole genome duplication |
| FRT2 |
YAL028W |
Protein HPH2; Tail-anchored ER membrane protein of unknown function; interacts with homolog Frt1p; promotes growth in conditions of high Na+, alkaline pH, or cell w stress, possibly via a role in posttranslational translocation; potential Cdc28p substrate; FRT2 has a paralog, FRT1, that arose from the whole genome duplication |
| SAW1 |
YAL027W |
5'- and 3'-flap DNA binding protein; recruits Rad1p-Rad10p to single-strand annealing intermediates with 3' non-homologous tails for removal during double-strand break repair; complexes with Rad1p-Rad10p and stimulates its endonuclease activity; green fluorescent protein (GFP)-fusion protein localizes to the nucleus |
| DRS2 |
YAL026C |
Probable phospholipid-transporting ATPase DRS2; Trans-golgi network aminophospholipid translocase (flippase); maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation, endocytosis, protein trafficking between the Golgi and endosomal system and the cellular response to mating pheromone; autoinhibited by its C-terminal tail; localizes to the trans-Golgi network; mutations in human homolog ATP8B1 result in liver disease; Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily |
| HRA1 |
YNCA0001W |
Unknown |
| MAK16 |
YAL025C |
Ribosome biosynthesis protein mak16; Essential nuclear protein; constituent of 66S pre-ribosomal particles; required for maturation of 25S and 5.8S rRNAs; required for maintenance of M1 satellite double-stranded RNA of the L-A virus |
| LTE1 |
YAL024C |
Guanine nucleotide exchange factor LTE1; Protein similar to GDP/GTP exchange factors; without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures |
| PMT2 |
YAL023C |
Dolichyl-phosphate-mannose--protein mannosyltransferase 2; Protein O-mannosyltransferase of the ER membrane; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; involved in ER quality control; acts in a complex with Pmt1p, can instead interact with Pmt5p; antifungal drug target; PMT2 has a paralog, PMT3, that arose from the whole genome duplication; Belongs to the glycosyltransferase 39 family |
| FUN26 |
YAL022C |
Nucleoside transporter FUN26; High affinity, broad selectivity, nucleoside/nucleobase transporter; vacuolar membrane localized transporter which may regulate the balance of nicotinamide riboside (NmR) levels between the cytosol and vacuole, contributing to salvage of NmR for use in cytosolic NAD+ synthesis; equilibrative nucleoside transporter (ENT) family member |
| CCR4 |
YAL021C |
Glucose-repressible alcohol dehydrogenase transcriptional effector; Component of the CCR4-NOT transcriptional complex; CCR4-NOT is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening; Belongs to the CCR4/nocturin family |
| ATS1 |
YAL020C |
Protein required for modification of wobble nucleosides in tRNA; acts with Elongator complex, Kti11p, and Kti12p; has a potential role in regulatory interactions between microtubules and the cell cycle; forms a stable heterodimer with Kti11p |
| FUN30 |
YAL019W |
Swi/snf-related matrix-associated actin-dependent regulator of chromatin subfamily a containing dead/h box 1; ATP-dependent helicase FUN30; Snf2p family member with ATP-dependent chromatin remodeling activity; has a role in silencing at the mating type locus, telomeres and centromeres; enriched at centromeres and is required for correct chromatin structure around centromeres, as well as at the boundary element of the silent HMR; recruited to DNA double-strand breaks (DSBs) where it promotes 5' strand resection of DSBs; potential Cdc28p substrate |
| LDS1 |
YAL018C |
Protein Involved in spore w assembly; localizes to lipid droplets found on or outside of the prospore membrane; shares similarity with Lds2p and Rrt8p, and a strain mutant for 3 genes exhibits reduced dityrosine fluorescence relative to the single mutants |
| PSK1 |
YAL017W |
Serine/threonine-protein kinase PSK1; PAS domain-containing serine/threonine protein kinase; coordinately regulates protein synthesis and carbohydrate metabolism and storage in response to a unknown metabolite that reflects nutritional status; PSK1 has a paralog, PSK2, that arose from the whole genome duplication |
| TPD3 |
YAL016W |
Regulatory subunit A of the heterotrimeric PP2A complex; the heterotrimeric protein phosphatase 2A (PP2A) complex also contains regulatory subunit Cdc55p and either catalytic subunit Pph21p or Pph22p; required for cell morphogenesis and transcription by RNA polymerase III |
| NTG1 |
YAL015C |
Endonuclease III homolog 1; DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; required for maintaining mitochondrial genome integrity; NTG1 has a paralog, NTG2, that arose from the whole genome duplication; Belongs to the Nth/MutY family |
| SYN8 |
YAL014C |
Syntaxin-8; Endosomal SNARE related to mammalian syntaxin 8; Belongs to the syntaxin family |
| DEP1 |
YAL013W |
Transcriptional regulatory protein DEP1; Component of the Rpd3L histone deacetylase complex; required for diauxic shift-induced histone H2B deposition onto rDNA genes; transcriptional modulator involved in regulation of structural phospholipid biosynthesis genes and metabolicy unrelated genes, as well as maintenance of telomeres, mating efficiency, and sporulation |
| CYS3 |
YAL012W |
Cystathionine gamma-lyase; catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress; Belongs to the trans-sulfuration enzymes family |
| SWC3 |
YAL011W |
SWR1-complex protein 3; Protein of unknown function; component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae; Belongs to the SWC3 family |
| MDM10 |
YAL010C |
Mitochondrial distribution and morphology protein 10; Subunit of both the ERMES and the SAM complex; component of ERMES complex which acts as a molecular tether between the mitochondria and the ER, necessary for efficient phospholipid exchange between organelles and for mitophagy; SAM/TOB complex component that functions in the assembly of outer membrane beta-barrel proteins; involved in mitochondrial inheritance and morphology; ERMES complex is often co-localized with peroxisomes and concentrated areas of pyruvate dehydrogenase; Belongs to the MDM10 family |
| SPO7 |
YAL009W |
Sporulation-specific protein SPO7; Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation |
| FUN14 |
YAL008W |
Protein FUN14; Integral mitochondrial outer membrane (MOM) protein; dosage suppressor of an MDM10 null that reduces ERMES-related phenotypes, such as alterations in mitochondrial morphology, protein complex assembly, and lipid profile; dosage suppressor of MDM12, MDM34, and MMM1 null mutant growth defects; novel mechanism of MOM import involving Tom70p, the TOM complex, and the TIM23 complex, requiring mitochondrial membrane potential and processing by the IMP complex for correct biogenesis; Belongs to the FUN14 family |
| ERP2 |
YAL007C |
Protein ERP2; Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; role in misfolded protein quality control; forms a heterotrimeric complex with Erp1p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP2 has a paralog, ERP4, that arose from the whole genome duplication |
| TRN1 |
YNCA0002W |
Unknown |
| SSA1 |
YAL005C |
Heat shock protein SSA1; ATPase involved in protein folding and NLS-directed nuclear transport; member of HSP70 family; required for ubiquitin-dependent degradation of short-lived proteins; forms chaperone complex with Ydj1p; localized to nucleus, cytoplasm, cell w; 98% identical to paralog Ssa2p with different functional specificity in propagation of yeast [URE3] prions, vacuolar-mediated degradations of gluconeogenesis enzymes; general targeting factor of Hsp104p to prion fibrils |
| EFB1 |
YAL003W |
Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site; human homolog EEF1B2 can complement yeast efb1 mutants; Belongs to the EF-1-beta/EF-1-delta family |
| SNR18 |
YNCA0003W |
Unknown |
| VPS8 |
YAL002W |
Vacuolar protein sorting-associated protein 8; Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif |
| TFC3 |
YAL001C |
Subunit of RNA polymerase III transcription initiation factor complex; part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding; largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC) |
| NUP60 |
YAR002W |
FG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of the nuclear pore complex (NPC) permeability barrier and is involved in gene tethering at the nuclear periphery; relocalizes to the cytosol in response to hypoxia; both NUP1 and NUP60 are homologous to human NUP153 |
| ERP1 |
YAR002C-A |
Protein ERP1; Member of the p24 family involved in ER to Golgi transport; role in misfolded protein quality control; forms heterotrimeric complex with Erp2p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP1 has a paralog, ERP6, that arose from the whole genome duplication |
| SWD1 |
YAR003W |
COMPASS component SWD1; Subunit of the COMPASS (Set1C) complex; COMPASS methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member with similarity to mammalian Rbbp7 |
| RFA1 |
YAR007C |
Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination; RPA protects against inappropriate telomere recombination, and upon telomere uncapping, prevents cell proliferation by a checkpoint-independent pathway; role in DNA catenation/decatenation pathway of chromosome disentangling; relocalizes to the cytosol in response to hypoxia |
| SEN34 |
YAR008W |
Subunit of the tRNA splicing endonuclease; tRNA splicing endonuclease (Sen complex) is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen complex also cleaves the CBP1 mRNA at the mitochondrial surface; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease |
| TGA1 |
YNCA0004W |
Unknown |
| BUD14 |
YAR014C |
Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; forms a complex with Kel1p and Kel2p that regulates Bnr1p (formin) to affect actin cable assembly, cytokinesis, and polarized growth; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress |
| ADE1 |
YAR015W |
Phosphoribosylaminoimidazole-succinocarboxamide synthase; N-succinyl-5-aminoimidazole-4-carboxamide ribotide synthetase; required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress |
| KIN3 |
YAR018C |
Nima (never in mitosis gene a)-related kinase 2; Serine/threonine-protein kinase KIN3; Nonessential serine/threonine protein kinase; possible role in DNA damage response; influences tolerance to high levels of ethanol |
| CDC15 |
YAR019C |
Cell division control protein 15; Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress |
| PAU7 |
YAR020C |
Seripauperin-7; Member of the seripauperin multigene family; active during alcoholic fermentation, regulated by anaerobiosis, inhibited by oxygen, repressed by heme; Belongs to the SRP1/TIP1 family. Seripauperin subfamily |
| YAR023C |
YAR023C |
Putative integral membrane protein; member of DUP240 gene family; Belongs to the DUP/COS family |
| SUP56 |
YNCA0005W |
Unknown |
| YNCA0006C |
YNCA0006C |
Unknown |
| UIP3 |
YAR027W |
ULP1-interacting protein 3; Putative integral membrane protein of unknown function; interacts with Ulp1p at the nuclear periphery; member of DUP240 gene family; Belongs to the DUP/COS family |
| YAR029W |
YAR029W |
DUP240 protein YAR029W; Member of DUP240 gene family but contains no transmembrane domains; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; Belongs to the DUP/COS family |
| YAR028W |
YAR028W |
DUP240 protein YAR028W; Putative integral membrane protein; member of DUP240 gene family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS |
| PRM9 |
YAR031W |
Pheromone-regulated protein; contains 3 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; member of DUP240 gene family; PRM9 has a paralog, PRM8, that arose from a segmental duplication |
| YAT1 |
YAR035W |
Outer mitochondrial carnitine acetyltransferase; minor ethanol-inducible enzyme involved in transport of activated acyl groups from the cytoplasm into the mitochondrial matrix; phosphorylated |
| YAR035C-A |
YAR035C-A |
Uncharacterized protein YAR035C-A; Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; predicted to have a role in cell budding based on computational "guilt by association" analysis |
| SWH1 |
YAR042W |
Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats and FFAT motif; interacts with ER anchor Scs2p at the nucleus-vacuole junction; regulated by sterol binding; SWH1 has a paralog, OSH2, that arose from the whole genome duplication; Belongs to the OSBP family |
| FLO5 |
YHR211W |
Flocculation protein FLO5; Lectin-like cell w protein (flocculin) involved in flocculation; binds mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin resistant but heat labile; important for co-flocculation with other yeasts, mediating interaction with specific species; FLO5 has a paralog, FLO1, that arose from a segmental duplication; Belongs to the flocculin family |
| YHR213W-A |
YHR213W-A |
Uncharacterized protein YHR213W-A; Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching |
| YHR213W-B |
YHR213W-B |
Uncharacterized protein YHR213W-B; Pseudogenic fragment with similarity to flocculins; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; YHR213W-B has a paralog, YAR064W, that arose from a segmental duplication |
| YHR214W |
YHR214W |
Putative protein of unknown function; predicted to be a glycosylphosphatidylinositol-modified (GPI) protein; YHR214W has a paralog, YAR066W, that arose from a segmental duplication |
| PAU10 |
YDR542W |
Seripauperin-10; Protein of unknown function; SWAT-GFP fusion protein localizes to the endoplasmic reticulum and vacuole, while mCherry fusion localizes to the vacuole; member of the seripauperin multigene family encoded mainly in subtelomeric regions; Belongs to the SRP1/TIP1 family. Seripauperin subfamily |
| YNCB0001W |
YNCB0001W |
Unknown |
| MIX23 |
YBL107C |
Mitochondrial intermembrane space protein of unknown function; imported via the MIA import machinery; contains an unusual twin cysteine motif (CX13C CX14C) |
| SRO77 |
YBL106C |
Lethal(2) giant larvae protein homolog SRO77; Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; regulates cell proliferation and colony development via the Rho1-Tor1 pathway; interacts with SNARE protein Sec9p; homolog of Drosophila lethal giant larvae tumor suppressor; SRO77 has a paralog, SRO7, that arose from the whole genome duplication |
| PKC1 |
YBL105C |
Protein serine/threonine kinase; essential for cell w remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) |
| SEA4 |
YBL104C |
Wd repeat-containing protein mio; SEH-associated protein 4; Subunit of SEACAT, a subcomplex of the SEA complex; Sea4p, along with Rtc1p and Mtc5p, redundantly inhibit the TORC1 inhibitory role of the Iml1p/SEACIT (Iml1p-Npr2p-Npr3p) subcomplex, a GAP for GTPase Gtr1p (EGOC subunit) in response to amino acid limitation, thereby resulting in activation of TORC1 signaling; SEA is a coatomer-related complex that associates dynamicy with the vacuole; contains an N-terminal beta-propeller fold and a C-terminal RING motif |
| RTG3 |
YBL103C |
Retrograde regulation protein 3; bHLH/Zip transcription factor for retrograde (RTG) and TOR pathways; forms a complex with another bHLH/Zip protein, Rtg1p, to activate the pathways; target of Hog1p |
| SFT2 |
YBL102W |
Tetra-spanning membrane protein found mostly in the late Golgi; non-essential; can suppress some sed5 eles; may be part of the transport machinery, but precise function is unknown; similar to mammalian syntaxin 5; Belongs to the SFT2 family |
| ECM21 |
YBL101C |
Arrestin-related trafficking adapter 2/8; Protein involved in regulating endocytosis of plasma membrane proteins; identified as a substrate for ubiquitination by Rsp5p and deubiquitination by Ubp2p; promoter contains several Gcn4p binding elements; ECM21 has a paralog, CSR2, that arose from the whole genome duplication |
| YBL100W-C |
YBL100W-C |
Uncharacterized protein YBL100W-C; Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching |
| YNCB0002W |
YNCB0002W |
Unknown |
| ATP1 |
YBL099W |
Alpha subunit of the F1 sector of mitochondrial F1F0 ATP synthase; which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; F1 translationy regulates ATP6 and ATP8 expression to achieve a balanced output of ATP synthase genes encoded in nucleus and mitochondria; phosphorylated; N-terminy propionylated in vivo; Belongs to the ATPase alpha/beta chains family |
| BNA4 |
YBL098W |
Kynurenine 3-monooxygenase; required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p; putative therapeutic target for Huntington disease |
| BRN1 |
YBL097W |
Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission |
| MRX3 |
YBL095W |
MIOREX complex component 3; Protein that associates with mitochondrial ribosome; likely functions in cristae junction formation; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies |
| ROX3 |
YBL093C |
Mediator of rna polymerase ii transcription subunit 19, fungi type; Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme |
| RPL32 |
YBL092W |
Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog |
| SCS22 |
YBL091C-A |
Vesicle-associated membrane protein-associated protein SCS22; Protein involved in regulation of phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect the ER and plasma membrane (PM); regulates PM PI4P levels by controlling access of the Sac1p phosphatase to its substrate, PI4P; human VAP homolog; similar to D. melanogaster inturned protein; SWAT-GFP and mCherry fusion proteins localize to the cytosol; SCS22 has a paralog, SCS2, that arose from the whole genome duplication; Belongs to the VAMP-associated protein (VAP) (TC 9.B.17) family |
| MAP2 |
YBL091C |
Methionine aminopeptidase; catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partiy redundant with that of Map1p; Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily |
| MRP21 |
YBL090W |
37S ribosomal protein MRP21, mitochondrial; Mitochondrial ribosomal protein of the sm subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences |
| AVT5 |
YBL089W |
Vacuolar amino acid transporter 5; Putative transporter; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters; AVT5 has a paralog, AVT6, that arose from the whole genome duplication |
| TEL1 |
YBL088C |
Serine/threonine-protein kinase TEL1; Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; acts with Red1p and Mec1p to promote interhomolog recombination by phosphorylation of Hop1; functiony redundant with Mec1p; regulates P-body formation induced by replication stress; homolog of human ataxia-telangiectasia mutated (ATM) gene; Belongs to the PI3/PI4-kinase family. ATM subfamily |
| RPL23A |
YBL087C |
Ribosomal 60S subunit protein L23A; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23A has a paralog, RPL23B, that arose from the whole genome duplication |
| YBL086C |
YBL086C |
Uncharacterized protein YBL086C; Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery |
| BOI1 |
YBL085W |
Protein implicated in polar growth; functiony redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication |
| CDC27 |
YBL084C |
Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition |
| ALG3 |
YBL082C |
Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase; Dolichol-P-Man dependent alpha(1-3) mannosyltransferase; involved in synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins; G353A missense mutation in human ortholog ALG3 implicated in carbohydrate deficient glycoprotein syndrome type IV, which is characterized by microcephaly, severe epilepsy, minimal psychomotor development, partial deficiency of sialic acids in serum glycoproteins; wild-type human ALG3 can complement yeast alg3 mutant; Belongs to the glycosyltransferase 58 family |
| YBL081W |
YBL081W |
Uncharacterized protein YBL081W; Non-essential protein of unknown function; null mutation results in a decrease in plasma membrane electron transport |
| PET112 |
YBL080C |
Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial; Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functiony complemented by the bacterial GatB ortholog; Belongs to the GatB/GatE family. GatB subfamily |
| NUP170 |
YBL079W |
Nucleoporin NUP170; Subunit of inner ring of nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; interacts with genomic regions that contain ribosomal protein and subtelomeric genes, where it functions in nucleosome positioning and as a repressor of transcription; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication |
| ATG8 |
YBL078C |
Autophagy-related protein 8; Component of autophagosomes and Cvt vesicles; regulator of Atg1p, targets it to autophagosomes; binds the Atg1p-Atg13p complex, triggering its vacuolar degradation; unique ubiquitin-like protein whose conjugation target is lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis |
| ILS1 |
YBL076C |
Isoleucine--tRNA ligase, cytoplasmic; Cytoplasmic isoleucine-tRNA synthetase; target of the G1-specific inhibitor reveromycin A |
| SSA3 |
YBL075C |
Heat shock protein SSA3; ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication |
| AAR2 |
YBL074C |
A1 cistron-splicing factor AAR2; Component of the U5 snRNP complex; required for splicing of U3 precursors; originy described as a splicing factor specificy required for splicing pre-mRNA of the MATa1 cistron; Belongs to the AAR2 family |
| SNR56 |
YNCB0003W |
Unknown |
| RPS8A |
YBL072C |
Protein component of the sm (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication |
| YBL071C-B |
YBL071C-B |
Uncharacterized protein YBL071C-B; Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching |
| KTI11 |
YBL071W-A |
Diphthamide biosynthesis protein 3; Zn-ribbon protein that co-purifies with Dph1 and Dph2; in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p; involved in modification of wobble nucleosides in tRNAs; forms a stable heterodimer with Ats1p; Belongs to the DPH3 family |
| YBL071C |
YBL071C |
Uncharacterized protein YBL071C; Putative protein of unknown function; conserved among S. cerevisiae strains; YBL071C is not an essential gene |
| AST1 |
YBL069W |
Putative uncharacterized protein ybl068w-a; Protein AST1; Lipid raft associated protein; interacts with the plasma membrane ATPase Pma1p and has a role in its targeting to the plasma membrane by influencing its incorporation into lipid rafts; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST1 has a paralog, AST2, that arose from the whole genome duplication |
| PRS4 |
YBL068W |
Ribose-phosphate pyrophosphokinase 4; 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP; which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS4 has a paralog, PRS2, that arose from the whole genome duplication; a missense mutation in the conserved residue R196 of its human homolog PRPS1 is pathogenic; Belongs to the ribose-phosphate pyrophosphokinase family |
| UBP13 |
YBL067C |
Ubiquitin carboxyl-terminal hydrolase 9/13; Ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication; Belongs to the peptidase C19 family |
| SEF1 |
YBL066C |
Putative transcription factor sef1; Putative transcription factor; has homolog in Kluyveromyces lactis |
| PRX1 |
YBL064C |
Mitochondrial peroxiredoxin with thioredoxin peroxidase activity; has a role in reduction of hydroperoxides; reactivation requires Trr2p and glutathione; induced during respiratory growth and oxidative stress; phosphorylated; protein abundance increases in response to DNA replication stress |
| KIP1 |
YBL063W |
Kinesin-like protein KIP1; Kinesin-related motor protein; required for mitotic spindle assembly, chromosome segregation, and 2 micron plasmid partitioning; functiony redundant with Cin8p for chromosomal but not plasmid functions; Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. BimC subfamily |
| SKT5 |
YBL061C |
Protein SKT5; Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication |
| YEL1 |
YBL060W |
Guanine nucleotide exchange factor specific for Arf3p; localized to the bud neck and tip; required for localization of Arf3p to the bud neck and tip |
| CMC2 |
YBL059C-A |
COX assembly mitochondrial protein 2; Protein involved in respiratory chain complex assembly or maintenance; protein of the mitochondrial intermembrane space; contains twin Cx9C motifs that can form coiled coil-helix-coiled-coil helix fold |
| IAI11 |
YBL059W |
Uncharacterized protein YBL059W; Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YBL059W has a paralog, YER093C-A, that arose from the whole genome duplication |
| SHP1 |
YBL058W |
UBX domain-containing substrate adaptor for Cdc48p; ubiquitin regulatory X domain-containing protein that acts as a substrate recruiting cofactor for Cdc48p; positively regulates Glc7p PPase activity to promote growth and mitotic progression in complex with Cdc48p; ubiquitinated protein interactor involved in ER-associated degradation (ERAD); regulated by nuclear Ub-dependent degradation (INMAD pathway) independent of the Asi and Doa10 complexes; homolog of human p47 (NSFL1C) |
| PTH2 |
YBL057C |
Peptidyl-trna hydrolase, pth2 family; Peptidyl-tRNA hydrolase 2; One of two mitochondriy-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth; see also PTH1 |
| PTC3 |
YBL056W |
Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p (see also Ptc2p) to limit maximal kinase activity induced by osmotic stress; dephosphorylates T169 phosphorylated Cdc28p (see also Ptc2p); role in DNA damage checkpoint inactivation; PTC3 has a paralog, PTC2, that arose from the whole genome duplication |
| YBL055C |
YBL055C |
3'-5'-exodeoxyribonuclease; Deoxyribonuclease Tat-D; 3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases |
| TOD6 |
YBL054W |
Transcriptional regulatory protein TOD6; PAC motif binding protein involved in rRNA and ribosome biogenesis; subunit of the RPD3L histone deacetylase complex; Myb-like HTH transcription factor; hypophosphorylated by rapamycin treatment in a Sch9p-dependent manner; activated in stochastic pulses of nuclear localization; Belongs to the DOT6 family |
| SAS3 |
YBL052C |
Histone acetyltransferase catalytic subunit of NuA3 complex; acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; mutant has aneuploidy tolerance; sas3gcn5 double mutation is lethal; Belongs to the MYST (SAS/MOZ) family |
| PIN4 |
YBL051C |
RNA-binding protein PIN4; Protein involved in G2/M phase progression and response to DNA damage; interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage |
| SEC17 |
YBL050W |
Alpha-soluble NSF attachment protein; Alpha-SNAP cochaperone; SNARE-complex adaptor for Sec18 (NSF) during the disassembly of postfusion cis-SNARE complexes; stimulates the ATPase activity of Sec18p; peripheral membrane protein required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, and autophagy; similar to mammalian alpha-SNAP |
| MOH1 |
YBL049W |
Protein yippee-like MOH1; Protein of unknown function, essential for stationary phase survival; not required for growth on nonfermentable carbon sources; possibly linked with vacuolar transport; Belongs to the yippee family |
| RRT1 |
YBL048W |
Regulator of rDNA transcription protein 1; Protein of unknown function; identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data |
| EDE1 |
YBL047C |
EH domain-containing and endocytosis protein 1; Scaffold protein involved in the formation of early endocytic sites; putative regulator of cytokinesis; homo-oligomerization is required for localization to and organization of endocytic sites; has a network of interactions with other endocytic proteins; binds membranes in a ubiquitin-dependent manner; may also bind ubiquitinated membrane-associated proteins; interacts with Cmk2 and functions upstream of CMK2 in regulating non-apoptotic cell death; homolog of mammalian Eps15; Belongs to the VDP/USO1/EDE1 family |
| PSY4 |
YBL046W |
Regulatory subunit of protein phosphatase PP4; presence of Psy4p in the PP4 complex (along with catalytic subunit Pph3p and Psy2p) is required for dephosphorylation of the histone variant H2AX, but not for dephosphorylation of Rad53p, during recovery from the DNA damage checkpoint; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; required for cisplatin resistance; homolog of mammalian R2 |
| COR1 |
YBL045C |
Cytochrome b-c1 complex subunit 1, mitochondrial; Core subunit of the ubiquinol-cytochrome c reductase complex; the ubiquinol-cytochrome c reductase complex (bc1 complex) is a component of the mitochondrial inner membrane electron transport chain |
| YBL044W |
YBL044W |
Uncharacterized protein ybl044w; Putative protein of unknown function; YBL044W is not an essential protein |
| ECM13 |
YBL043W |
Non-essential protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation; ECM13 has a paralog, YJR115W, that arose from the whole genome duplication |
| FUI1 |
YBL042C |
Nucleobase:cation symporter-1, ncs1 family; High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress |
| PRE7 |
YBL041W |
Proteasome core particle subunit beta 6; Beta 6 subunit of the 20S proteasome |
| ERD2 |
YBL040C |
ER lumen protein-retaining receptor; HDEL receptor; an integral membrane protein that binds to the HDEL motif in proteins destined for retention in the endoplasmic reticulum; has a role in maintenance of normal levels of ER-resident proteins |
| MIN6 |
YBL039W-B |
Uncharacterized protein YBL039W-B; Putative protein of unknown function; mCherry fusion protein localizes to the vacuole |
| URA7 |
YBL039C |
Major CTP synthase isozyme (see also URA8); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especiy during conditions of glucose depletion; URA7 has a paralog, URA8, that arose from the whole genome duplication |
| MRPL16 |
YBL038W |
Mitochondrial ribosomal protein of the large subunit; homologous to bacterial L16 ribosomal protein; synthetic lethality with hac1 mutation suggests a possible role in synthesis of precursors for protein glycosylation |
| APL3 |
YBL037W |
Alpha-adaptin; large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport |
| YBL036C |
YBL036C |
Pyridoxal phosphate homeostasis protein; Putative non-specific single-domain racemase; based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS |
| POL12 |
YBL035C |
B subunit of DNA polymerase alpha-primase complex; required for initiation of DNA replication during mitotic and premeiotic DNA synthesis; also functions in telomere capping and length regulation |
| STU1 |
YBL034C |
Protein STU1; Component of the mitotic spindle; binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles; Belongs to the CLASP family |
| RIB1 |
YBL033C |
GTP cyclohydrolase II; catalyzes the first step of the riboflavin biosynthesis pathway |
| HEK2 |
YBL032W |
Heterogeneous nuclear rnp K-like protein 2; RNA binding protein involved in asymmetric localization of ASH1 mRNA; represses translation of ASH1 mRNA, an effect reversed by Yck1p-dependent phosphoryation; regulates telomere position effect and length; similarity to hnRNP-K |
| SHE1 |
YBL031W |
Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function |
| PET9 |
YBL030C |
Solute carrier family 25 (mitochondrial adenine nucleotide translocator), member 4/5/6/31; ADP,ATP carrier protein 2; Major ADP/ATP carrier of the mitochondrial inner membrane; exchanges cytosolic ADP for mitochondriy synthesized ATP; also imports heme and ATP; required for viability in many lab strains that carry a sal1 mutation; PET9 has a paralog, AAC3, that arose from the whole genome duplication; human homolog SLC25A4 implicated in progressive external ophthalmoplegia can complement yeast null mutant |
| YBL029C-A |
YBL029C-A |
UPF0768 protein YBL029C-A; Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress; has potential orthologs in Saccharomyces species and in Yarrowia lipolytica; Belongs to the UPF0768 family |
| YBL029W |
YBL029W |
Uncharacterized protein YBL029W; Non-essential protein of unknown function |
| YBL028C |
YBL028C |
UPF0642 protein YBL028C; Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis |
| RPL19B |
YBL027W |
Ribosomal 60S subunit protein L19B; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19B has a paralog, RPL19A, that arose from the whole genome duplication |
| LSM2 |
YBL026W |
U6 snRNA-associated Sm-like protein LSm2; Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress |
| RRN10 |
YBL025W |
Rna polymerase i-specific transcription initiation factor rrn10; Protein involved in promoting high level transcription of rDNA; subunit of UAF (upstream activation factor) for RNA polymerase I |
| NCL1 |
YBL024W |
Multisite-specific tRNA:(cytosine-C(5))-methyltransferase; S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase; methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120 |
| MCM2 |
YBL023C |
Protein involved in DNA replication; component of the Mcm2-7 hexameric helicase complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress |
| PIM1 |
YBL022C |
ATP-dependent Lon protease; involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria; Belongs to the peptidase S16 family |
| HAP3 |
YBL021C |
Transcriptional activator HAP3; Subunit of the Hap2p/3p/4p/5p CCAAT-binding complex; complex is heme-activated and glucose-repressed; complex is a transcriptional activator and global regulator of respiratory gene expression; contains sequences contributing to both complex assembly and DNA binding |
| RFT1 |
YBL020W |
Membrane protein required for translocation of Man5GlcNac2-PP-Dol; required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein; essential gene |
| APN2 |
YBL019W |
DNA-(apurinic or apyrimidinic site) lyase 2; Class II abasic (AP) endonuclease involved in repair of DNA damage; homolog of human HAP1 and E. coli exoIII |
| POP8 |
YBL018C |
Ribonucleases P/MRP protein subunit POP8; Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; relocalizes to the cytosol in response to hypoxia |
| PEP1 |
YBL017C |
Vacuolar protein sorting/targeting protein VPS10; Type I transmembrane sorting receptor for multiple vacuolar hydrolases; cycles between the late-Golgi and prevacuolar endosome-like compartments; Belongs to the VPS10-related sortilin family |
| FUS3 |
YBL016W |
Mitogen-activated serine/threonine protein kinase involved in mating; phosphoactivated by Ste7p; substrates include Ste12p, Far1p, Bni1p, Sst2p; inhibits invasive growth during mating by phosphorylating Tec1p, promoting its; inhibits recruitment of Ste5p, Cdc42p-mediated asymmetry and mating morphogenesis; Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily |
| ACH1 |
YBL015W |
Acetyl-CoA hydrolase; Protein with CoA transferase activity; particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth; Belongs to the acetyl-CoA hydrolase/transferase family |
| YNCB0004W |
YNCB0004W |
Unknown |
| YNCB0018W |
YNCB0018W |
Unknown |
| RRN6 |
YBL014C |
RNA polymerase I-specific transcription initiation factor RRN6; Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p |
| FMT1 |
YBL013W |
Methionyl-tRNA formyltransferase, mitochondrial; Methionyl-tRNA formyltransferase; catalyzes the formylation of initiator Met-tRNA in mitochondria; potential Cdc28p substrate |
| SCT1 |
YBL011W |
Glycerol-3-phosphate O-acyltransferase 1; Glycerol 3-phosphate/dihydroxyacetone phosphate sn-1 acyltransferase; dual substrate-specific acyltransferase of the glycerolipid biosynthesis pathway; prefers 16-carbon fatty acids; similar to Gpt2p; gene is constitutively transcribed |
| LAA2 |
YBL010C |
Uncharacterized protein YBL010C; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles; Belongs to the bZIP family |
| ALK2 |
YBL009W |
Serine/threonine-protein kinase Haspin homolog ALK2; Protein kinase; along with its paralog, ALK1, required for proper spindle positioning and nuclear segregation following mitotic arrest, proper organization of cell polarity factors in mitosis, proper localization of formins and polarity factors, and survival in cells that activate spindle assembly checkpoint; phosphorylated in response to DNA damage; ALK2 has a paralog, ALK1, that arose from the whole genome duplication; similar to mammalian haspins |
| YBL008W-A |
YBL008W-A |
Uncharacterized protein YBL008W-A; Putative protein of unknown function; identified by fungal homology and RT-PCR |
| HIR1 |
YBL008W |
Protein HIR1; Subunit of the HIR complex; HIR is a nucleosome assembly complex involved in regulation of histone gene transcription; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores |
| SLA1 |
YBL007C |
Actin cytoskeleton-regulatory complex protein SLA1; Cytoskeletal protein binding protein; required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains; Belongs to the SLA1 family |
| LDB7 |
YBL006C |
Chromatin structure-remodeling complex protein RSC14; Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions |
| PDR3 |
YBL005W |
Transcription factor PDR3; Transcriptional activator of the pleiotropic drug resistance network; regulates expression of ATP-binding cassette (ABC) transporters through binding to cis-acting PDRE sites (PDR responsive elements); has a role in response to drugs and organic solvents; post-translationy up-regulated in cells lacking functional mitochondrial genome; involved in diauxic shift; relative distribution to nucleus increases upon DNA replication stress; APCC(Cdh1) substrate |
| YNCB0006W |
YNCB0006W |
Unknown |
| UTP20 |
YBL004W |
U3 sm nucleolar RNA-associated protein 20; Component of the sm-subunit (SSU) processome; SSU processome is involved in the biogenesis of the 18S rRNA; Belongs to the UTP20 family |
| HTA2 |
YBL003C |
Histone H2A; core histone protein required for chromatin assembly and chromosome function; one of two nearly identical (see also HTA1) subtypes; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p |
| HTB2 |
YBL002W |
Histone H2B; core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB1; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates reassembly after DNA replication, transcriptional activation, meiotic DSB formation and H3 methylation |
| ECM15 |
YBL001C |
UPF0045 protein ECM15; Non-essential protein of unknown function; likely exists as tetramer, may be regulated by the binding of sm-molecule ligands (possibly sulfate ions), may have a role in yeast cell-w biogenesis |
| NTH2 |
YBR001C |
Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication; Belongs to the glycosyl hydrolase 37 family |
| RER2 |
YBR002C |
Forms the dehydrodolichyl diphosphate syntase (DDS) complex with NUS1; major enzyme of polyprenol synthesis in both the endoplasmic reticulum (ER) and in lipid droplets; participates in ER protein sorting; human ortholog DHDDS functiony complements the heat sensitive growth defect of a ts ele, and is associated with retinitis pigmentosa |
| COQ1 |
YBR003W |
Hexaprenyl pyrophosphate synthetase; catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis; Belongs to the FPP/GGPP synthase family |
| GPI18 |
YBR004C |
Gpi-anchor transamidase gpi18; GPI mannosyltransferase 2; Functional ortholog of human PIG-V; PIG-V is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria; Belongs to the PIGV family |
| RCR1 |
YBR005W |
Protein of the ER membrane involved in cell w chitin deposition; may function in the endosomal-vacuolar trafficking pathway, helping determine whether plasma membrane proteins are degraded or routed to the plasma membrane; RCR1 has a paralog, RCR2, that arose from the whole genome duplication |
| UGA2 |
YBR006W |
Succinate-semialdehyde dehydrogenase [NADP(+)]; Succinate semialdehyde dehydrogenase; involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm |
| DSF2 |
YBR007C |
Protein DSF2; Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress |
| FLR1 |
YBR008C |
Fluconazole resistance protein 1; Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; involved in efflux of fluconazole, diazaborine, benomyl, methotrexate, and other drugs; expression induced in cells treated with the mycotoxin patulin; relocalizes from nucleus to plasma membrane upon DNA replication stress |
| HHF1 |
YBR009C |
Histone H4; core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity |
| HHT1 |
YBR010W |
Histone H3; core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT2); regulated by acetylation, methylation, and phosphorylation; H3K14 acetylation plays an important role in the unfolding of strongly positioned nucleosomes during repair of UV damage |
| IPP1 |
YBR011C |
Inorganic diphosphatase ipp1; Cytoplasmic inorganic pyrophosphatase (PPase); homodimer that catalyzes the rapid exchange of oxygens from Pi with water, highly expressed and essential for viability, active-site residues show identity to those from E. coli PPase |
| YBR013C |
YBR013C |
Putative protein of unknown function; haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein; SWAT-GFP fusion protein localizes to the endoplasmic reticulum while mCherry fusion protein localizes to both the endoplasmic reticulum and vacuole |
| YNCB0007W |
YNCB0007W |
Unknown |
| GRX7 |
YBR014C |
Cis-golgi localized monothiol glutaredoxin; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; does not bind metal ions; GRX7 has a paralog, GRX6, that arose from the whole genome duplication |
| MNN2 |
YBR015C |
Alpha-1,2-mannosyltransferase; responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment; Belongs to the MNN1/MNT family |
| YBR016W |
YBR016W |
Uncharacterized protein YBR016W; Tail-anchored plasma membrane protein with a conserved CYSTM module; predicted to be palmitoylated; has similarity to hydrophilins, which are involved in the adaptive response to hyperosmotic conditions; YBR016W has a paralog, YDL012C, that arose from the whole genome duplication |
| KAP104 |
YBR017C |
Importin subunit beta-2; Transportin or cytosolic karyopherin beta 2; functions in the rg-nuclear localization signal-mediated nuclear import/reimport of mRNA-binding proteins Nab2p and Hrp1p; regulates asymmetric protein synthesis in daughter cells during mitosis; Belongs to the importin beta family. Importin beta-2 subfamily |
| GAL7 |
YBR018C |
UDPglucose--hexose-1-phosphate uridylyltransferase; Galactose-1-phosphate uridyl transferase; synthesizes glucose-1-phosphate and UDP-galactose from UDP-D-glucose and alpha-D-galactose-1-phosphate in the second step of galactose catabolism; human homolog UGP2 can complement yeast null mutant |
| GAL10 |
YBR019C |
Bifunctional protein GAL10; UDP-glucose-4-epimerase; catalyzes interconversion of UDP-galactose and UDP-D-glucose in galactose metabolism; also catalyzes conversion of alpha-D-glucose or alpha-D-galactose to their beta-anomers; human homolog GALE implicated in galactosemia, can complement yeast null mutant |
| YNCB0008W |
YNCB0008W |
Unknown |
| GAL1 |
YBR020W |
Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; human homolog GALK2 complements yeast null mutant; GAL1 has a paralog, GAL3, that arose from the whole genome duplication |
| FUR4 |
YBR021W |
Nucleobase:cation symporter-1, ncs1 family; Uracil permease; Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation |
| POA1 |
YBR022W |
Phosphatase that is highly specific for ADP-ribose 1''-phosphate; a tRNA splicing metabolite; may have a role in regulation of tRNA splicing; Belongs to the POA1 family |
| CHS3 |
YBR023C |
Chitin synthase III; catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell w chitin, the chitin ring during bud emergence, and spore w chitosan; contains overlapping di-leucine and di-acidic signals that mediate, respectively, intracellular trafficking by AP-1 and trafficking to plasma membrane by exomer complex; requires AP-3 complex for its intracellular retention |
| SCO2 |
YBR024W |
Putative thioredoxin peroxidase sco2; Protein anchored to mitochondrial inner membrane; may have a redundant function with Sco1p in delivery of copper to cytochrome c oxidase; interacts with Cox2p; SCO2 has a paralog, SCO1, that arose from the whole genome duplication |
| OLA1 |
YBR025C |
Obg-like ATPase 1; P-loop ATPase with similarity to human OLA1 and bacterial YchF; identified as specificy interacting with the proteasome; null mutant displays increased translation rate and increased readthrough of premature stop codons; protein abundance increases in response to hydrogen peroxide and to DNA replication stress; Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family. YchF/OLA1 subfamily |
| ETR1 |
YBR026C |
Enoyl-[acyl-carrier-protein] reductase, mitochondrial; 2-enoyl thioester reductase; member of the medium chain dehydrogenase/reductase family; localized to mitochondria, where it has a probable role in fatty acid synthesis; human MECR functiony complements the respiratory growth defect of the null mutant |
| YBR027C |
YBR027C |
Uncharacterized protein YBR027C; Putative protein of unknown function; conserved among S. cerevisiae strains; YBR027C is not an essential gene |
| YPK3 |
YBR028C |
Serine/threonine-protein kinase YPK3; AGC kinase; phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner; directly phosphorylated by TORC1; phosphorylates ribosomal protein Rps6a/b (S6), in a TORC-dependent manner; undergoes autophosphorylation |
| CDS1 |
YBR029C |
Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of major yeast phospholipids; human homolog CDS1 can complement yeast cds1 null mutant |
| RKM3 |
YBR030W |
Ribosomal lysine methyltransferase; specific for monomethylation of Rpl42ap and Rpl42bp (lysine 40); nuclear SET domain containing protein; relocalizes to the cytosol in response to hypoxia |
| RPL4A |
YBR031W |
Ribosomal 60S subunit protein L4A; N-terminy acetylated; homologous to mammalian ribosomal protein L4 and bacterial L4; RPL4A has a paralog, RPL4B, that arose from the whole genome duplication |
| YBR032W |
YBR032W |
Uncharacterized protein YBR032W; Putative protein of unknown function; conserved among S. cerevisiae strains; YBR032W is not an essential gene |
| EDS1 |
YBR033W |
Transcriptional regulatory protein EDS1; Putative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication; Belongs to the EDS1/RGT1 family |
| HMT1 |
YBR034C |
Protein-arginine omega-n methyltransferase hmt1; Protein arginine N-methyltransferase 1; Nuclear SAM-dependent mono- and asymmetric methyltransferase; modifies hnRNPs, including Npl3p and Hrp1p, affecting their activity and nuclear export; methylates U1 snRNP protein Snp1p, ribosomal protein Rps2p, and histones H3 and H4; interacts geneticy with genes encoding components of Rpd3(L) and this interaction is important for Rpd3 recruitment to the subtelomeric region |
| PDX3 |
YBR035C |
Pyridoxine (pyridoxamine) phosphate oxidase; has homologs in E. coli and Myxococcus xanthus; transcription is under the general control of nitrogen metabolism |
| SNR161 |
YNCB0009C |
Unknown |
| TLC1 |
YNCB0010W |
Unknown |
| CSG2 |
YBR036C |
Mannosyl phosphorylinositol ceramide synthase regulatory protein CSG2; Endoplasmic reticulum membrane protein; required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress |
| SCO1 |
YBR037C |
Copper-binding protein of mitochondrial inner membrane; required for cytochrome c oxidase activity and respiration; may function to deliver copper to cytochrome c oxidase; similar to thioredoxins; SCO1 has a paralog, SCO2, that arose from the whole genome duplication |
| CHS2 |
YBR038W |
Chitin synthase II; catalyzes transfer of N-acetylglucosamine (GlcNAc) to chitin upon activation of zymogenic form; required for chitin synthesis in the primary septum during cytokinesis; localization regulated by Cdk1p during mitosis; phosphorylation by Dbf2p kinase regulates its dynamics and chitin synthesis during cytokinesis |
| ATP3 |
YBR039W |
F-type h+-transporting atpase subunit gamma; Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis |
| FIG1 |
YBR040W |
Factor-induced gene 1 protein; Integral membrane protein required for efficient mating; may participate in or regulate the low affinity Ca2+ influx system, which affects intracellular signaling and cell-cell fusion during mating |
| FAT1 |
YBR041W |
Very long chain fatty acyl-CoA synthetase and fatty acid transporter; activates imported fatty acids with a preference for very long lengths (C20-C26); has a separate function in the transport of long chain fatty acids |
| CST26 |
YBR042C |
Uncharacterized acyltransferase CST26; Acyltransferase; enzyme mainly responsible for the introduction of saturated very long chain fatty acids into neo-synthesized molecules of phosphatidylinositol; required for incorporation of stearic acid into phosphatidylinositol; affects chromosome stability when overexpressed; CST26 has a paralog, YDR018C, that arose from the whole genome duplication |
| QDR3 |
YBR043C |
Quinidine resistance protein 3; Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; has a role in polyamine homeostasis; involved in spore w asembly; sequence similarity to DTR1 and QDR1, and the triple mutant dtr1 qdr1 qdr3 exhibits reduced dityrosine fluorescence relative to the single mutants; expression is upregulated under polyamine stress; required for resistance to quinidine, barban, cisplatin, and bleomycin |
| TCM62 |
YBR044C |
Mitochondrial chaperone TCM62; Protein involved in assembly of the succinate dehydrogenase complex; mitochondrial; putative chaperone |
| YNCB0011C |
YNCB0011C |
Unknown |
| GIP1 |
YBR045C |
GLC7-interacting protein 1; Meiosis-specific regulatory subunit of the Glc7p protein phosphatase; regulates spore w formation and septin organization, required for expression of some late meiotic genes and for normal localization of Glc7p |
| ZTA1 |
YBR046C |
Probable quinone oxidoreductase; NADPH-dependent quinone reductase; GFP-tagged protein localizes to the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystin |
| FMP23 |
YBR047W |
Protein FMP23, mitochondrial; Putative protein of unknown function; proposed to be involved in iron or copper homeostasis; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies |
| RPS11B |
YBR048W |
Protein component of the sm (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11B has a paralog, RPS11A, that arose from the whole genome duplication |
| REB1 |
YBR049C |
DNA-binding protein REB1; RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; Reb1p bound to DNA acts to block RNA polymerase II readthrough transcription |
| REG2 |
YBR050C |
Protein REG2; Regulatory subunit of the Glc7p type-1 protein phosphatase; involved with Reg1p, Glc7p, and Snf1p in regulation of glucose-repressible genes, also involved in glucose-induced proteolysis of maltose permease; REG2 has a paralog, REG1, that arose from the whole genome duplication |
| RFS1 |
YBR052C |
Protein of unknown function; member of a flavodoxin-like fold protein family that includes Pst2p and Ycp4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; RFS1 has a paralog, PST2, that arose from the whole genome duplication |
| YBR053C |
YBR053C |
Uncharacterized protein YBR053C; Putative protein of unknown function; induced by cell w perturbation |
| YRO2 |
YBR054W |
Protein with a putative role in response to acid stress; null mutant is sensitive to acetic acid; transcription is regulated by Haa1p and induced in the presence of acetic acid; protein observed in plasma membrane foci in the presence of acetic acid; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; Belongs to the archaeal/bacterial/fungal opsin family |
| PRP6 |
YBR055C |
U4/U6-U5 snRNP complex subunit PRP6; Splicing factor; component of the U4/U6-U5 snRNP complex |
| YNCB0012W |
YNCB0012W |
Unknown |
| MRX18 |
YBR056W |
17-beta-hydroxysteroid dehydrogenase-like protein; Uncharacterized glycosyl hydrolase YBR056W; Putative glycoside hydrolase of the mitochondrial intermembrane space; Belongs to the glycosyl hydrolase 5 (cellulase A) family |
| YNCB0013W |
YNCB0013W |
Unknown |
| MNC1 |
YBR056W-A |
Mnc1p; Uncharacterized protein YBR056W-A; Protein of unknown function; mRNA identified as translated by ribosome profiling data; partiy overlaps dubious ORF YBR056C-B; YBR056W-A has a paralog, YDR034W-B, that arose from the whole genome duplication |
| MUM2 |
YBR057C |
Protein essential for meiotic DNA replication and sporulation; cytoplasmic protein; subunit of the MIS complex which controls mRNA methylation during during the induction of sporulation; also interacts with Orc2p, which is a component of the origin recognition complex; Belongs to the fl(2)d family |
| UBP14 |
YBR058C |
Ubiquitin carboxyl-terminal hydrolase 14; Ubiquitin-specific protease; specificy disassembles unanchored ubiquitin chains; involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; similar to human isopeptidase T; Belongs to the peptidase C19 family |
| TSC3 |
YBR058C-A |
Protein that stimulates the activity of serine palmitoyltransferase; involved in sphingolipid biosynthesis; Lcb1p and Lcb2p are the two components of serine palmitoyltransferase |
| AKL1 |
YBR059C |
Serine/threonine-protein kinase AKL1; Ser-Thr protein kinase; member (with Ark1p and Prk1p) of the Ark kinase family; involved in endocytosis and actin cytoskeleton organization |
| ORC2 |
YBR060C |
Subunit of the origin recognition complex (ORC); ORC directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; interacts with Spp1p and with trimethylated histone H3; phosphorylated by Cdc28p |
| TRM7 |
YBR061C |
2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively; yeast null mutant can be functiony complemented by human FTSJ1, mutations in which have been implicated in nonsyndromic X-linked intellectual disability (NSXLID); Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family. TRM7 subfamily |
| YBR062C |
YBR062C |
Uncharacterized RING finger protein YBR062C; Protein of unknown function that interacts with Msb2p; may play a role in activation of the filamentous growth pathway |
| YBR063C |
YBR063C |
Uncharacterized protein ybr063c; Putative protein of unknown function; YBR063C is not an essential gene |
| ECM2 |
YBR065C |
Pre-mRNA-splicing factor SLT11; Pre-mRNA splicing factor; facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p; Belongs to the SLT11 family |
| NRG2 |
YBR066C |
Probable transcriptional regulator NRG2; Transcriptional repressor; mediates glucose repression and negatively regulates filamentous growth; activated in stochastic pulses of nuclear localization in response to low glucose |
| TIP1 |
YBR067C |
Temperature shock-inducible protein 1; Major cell w mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins |
| BAP2 |
YBR068C |
Leu/Val/Ile amino-acid permease; High-affinity leucine permease; functions as a branched-chain amino acid permease involved in uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains; BAP2 has a paralog, BAP3, that arose from the whole genome duplication; Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family |
| TAT1 |
YBR069C |
Valine/tyrosine/tryptophan amino-acid permease 1; Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress |
| TBRT |
YNCB0014W |
Unknown |
| ALG14 |
YBR070C |
UDP-N-acetylglucosamine transferase subunit ALG14; Component of UDP-GlcNAc transferase; required for second step of dolichyl-linked oligosaccharide synthesis; anchors catalytic subunit Alg13p to ER membrane; similar to bacterial and human glycosyltransferases; both human homologs ALG13 and ALG14 are required to complement yeast alg14 mutant |
| YBR071W |
YBR071W |
Uncharacterized protein YBR071W; Protein of unknown function found in the cytoplasm and bud neck; mRNA expression may be regulated by the cell cycle and/or cell w stress; overexpression of YBR071W affects endocytic protein trafficking |
| HSP26 |
YBR072W |
Sm heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; long-lived protein that is preferentiy retained in mother cells and forms cytoplasmic foci; oligomer activation requires heat-induced conformational change; also has mRNA binding activity |
| YBR072C-A |
YBR072C-A |
Uncharacterized protein YBR072C-A; Putative protein of unknown function; identified by fungal homology and RT-PCR |
| RDH54 |
YBR073W |
DNA-dependent ATPase; DNA recombination/repair translocase, supercoils DNA and promotes DNA strand opening; stimulates strand exchange by modifying dsDNA topology; involved in recombinational repair of DNA double-strand breaks (DSBs) during mitosis and meiosis; phosphorylated in Mec1p-, Rad53p-dependent way in response to one DSB; contributes to remodelling of nucleosomes; proposed to be involved in crossover interference; interacts with Dmc1p; stimulates Dmc1p and Rad51p |
| PFF1 |
YBR074W |
Multi-spanning vacuolar membrane protease; glycosylated transmembrane protein bearing homology to the M28 family of metoproteases; has a lumenal-facing protease domain; proposed role in vacuole physiology |
| ECM8 |
YBR076W |
Protein ecm8; May be involved in cell w organization and biogenesis |
| SLM4 |
YBR077C |
Protein SLM4; Component of the EGO and GSE complexes; essential for integrity and function of EGO; EGO is involved in the regulation of microautophagy and GSE is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4 |
| ECM33 |
YBR078W |
Cell w protein ECM33; GPI-anchored protein of unknown function; possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p; ECM33 has a paralog, PST1, that arose from the whole genome duplication |
| RPG1 |
YBR079C |
eIF3a subunit of the eukaryotic translation initiation factor 3 (eIF3); subunit of the core complex of eIF3; essential for translation; part of a Prt1p-Rpg1p-Nip1p subcomplex that stimulates binding of mRNA and tRNA(i)Met to ribosomes; involved in translation reinitiation; eIF3 is also involved in programmed stop codon readthrough |
| SEC18 |
YBR080C |
Vesicular-fusion protein SEC18; AAA ATPase and SNARE disassembly chaperone; required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, autophagy, and protein secretion; releases Sec17p from SNAP complexes; has similarity to mammalian N-ethylmaleimide-sensitive factor (NSF) |
| SPT7 |
YBR081C |
Transcriptional activator SPT7; Subunit of the SAGA transcriptional regulatory complex; involved in proper assembly of the complex; also present as a C-terminy truncated form in the SLIK/SALSA transcriptional regulatory complex |
| YNCB0015W |
YNCB0015W |
Unknown |
| UBC4 |
YBR082C |
Ubiquitin-conjugating enzyme (E2); key E2 partner with Ubc1p for the anaphase-promoting complex (APC); mediates degradation of abnormal or excess proteins, including calmodulin and histone H3; regulates levels of DNA Polymerase-{alpha} to promote efficient and accurate DNA replication; interacts with many SCF ubiquitin protein ligases; component of the cellular stress response; UBC4 has a paralog, UBC5, that arose from the whole genome duplication |
| TEC1 |
YBR083W |
Transcription factor targeting filamentation genes and Ty1 expression; Ste12p activation of most filamentation gene promoters depends on Tec1p and Tec1p transcriptional activity is dependent on its association with Ste12p; binds to TCS elements upstream of filamentation genes, which are regulated by Tec1p/Ste12p/Dig1p complex; competes with Dig2p for binding to Ste12p/Dig1p; positive regulator of chronological life span; TEA/ATTS DNA-binding domain family member; Belongs to the TEC1 family |
| MIS1 |
YBR084W |
Trifunctional formate-tetrahydrofolate ligase/methenyltetrahydrofolate cyclohydrolase/methylenetetrahydrofolate dehydrogenase mis1; C-1-tetrahydrofolate synthase, mitochondrial; Mitochondrial C1-tetrahydrofolate synthase; involved in interconversion between different oxidation states of tetrahydrofolate (THF); provides activities of formyl-THF synthetase, methenyl-THF cyclohydrolase, and methylene-THF dehydrogenase |
| RPL19A |
YBR084C-A |
Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication |
| AAC3 |
YBR085W |
Solute carrier family 25 (mitochondrial adenine nucleotide translocator), member 4/5/6/31; ADP,ATP carrier protein 3; Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondriy synthesized ATP; expressed under anaerobic conditions; similar to Aac1p; has roles in maintenance of viability and in respiration; AAC3 has a paralog, PET9, that arose from the whole genome duplication; Belongs to the mitochondrial carrier (TC 2.A.29) family |
| YBR085C-A |
YBR085C-A |
Uncharacterized protein YBR085C-A; Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus; protein abundance increases in response to DNA replication stress |
| IST2 |
YBR086C |
Increased sodium tolerance protein 2; Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localizes to the mother cell in sm-budded cells and to the bud in medium- and large-budded cells; mRNA is transported to the bud tip by an actomyosin-driven process |
| RFC5 |
YBR087W |
Subunit of heteropentameric Replication factor C (RF-C); RF-C is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon |
| POL30 |
YBR088C |
Proliferating cell nuclear antigen (PCNA); functions as the sliding replication clamp for DNA polymerase delta; may function as a docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair; PCNA ubiquitination at K164 plays a crucial role during Okazaki fragment processing |
| NHP6B |
YBR089C-A |
Non-histone chromosomal protein 6B; High-mobility group (HMG) protein; binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to the chromosomes; functiony redundant with Nhp6Ap; required for transcriptional initiation fidelity of some tRNA genes; homologous to mammalian HMGB1 and HMGB2; NHP6B has a paralog, NHP6A, that arose from the whole genome duplication |
| YBR090C |
YBR090C |
Uncharacterized protein YBR090C; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus |
| TIM12 |
YBR091C |
Mitochondrial import inner membrane translocase subunit TIM12; Essential protein of the inner mitochondrial membrane; periphery localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins |
| PHO3 |
YBR092C |
Constitutively expressed acid phosphatase similar to Pho5p; brought to the cell surface by transport vesicles; hydrolyzes thiamin phosphates in the periplasmic space, increasing cellular thiamin uptake; expression is repressed by thiamin |
| PHO5 |
YBR093C |
Repressible acid phosphatase; 1 of 3 repressible acid phosphatases that also mediates extracellular nucleotide-derived phosphate hydrolysis; secretory pathway derived cell surface glycoprotein; induced by phosphate starvation and coordinately regulated by PHO4 and PHO2; Belongs to the histidine acid phosphatase family |
| PBY1 |
YBR094W |
Probable tubulin--tyrosine ligase PBY1; Putative tubulin tyrosine ligase associated with P-bodies; may have a role in mRNA metabolism; yeast knockout collection strain identified as a pby1 null mutant is actuy wild-type for PBY1 and deleted for mms4; Belongs to the tubulin--tyrosine ligase family |
| RXT2 |
YBR095C |
Transcriptional regulatory protein RXT2; Component of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth; relocalizes to the cytosol in response to hypoxia |
| YBR096W |
YBR096W |
Uncharacterized protein YBR096W; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER |
| VPS15 |
YBR097W |
Serine/threonine-protein kinase VPS15; Serine/threonine protein kinase involved in vacuolar protein sorting; functions as a membrane-associated complex with Vps34p; active form recruits Vps34p to the Golgi membrane; interacts with the GDP-bound form of Gpa1p; myristoylated; a fraction is localized, with Vps34p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery |
| MMS4 |
YBR098W |
Crossover junction endonuclease eme1; Subunit of structure-specific Mms4p-Mus81p endonuclease; cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdc5p during mitotic cell cycle activates the function of Mms4p-Mus81p |
| FES1 |
YBR101C |
Hsp70 (Ssa1p) nucleotide exchange factor; required for the release of misfolded proteins from the Hsp70 system to the Ub-proteasome machinery for destruction; cytosolic homolog of Sil1p, which is the nucleotide exchange factor for BiP (Kar2p) in the endoplasmic reticulum; protein abundance increases in response to DNA replication stress |
| EXO84 |
YBR102C |
Exocyst complex component EXO84; Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate; Belongs to the EXO84 family |
| SIF2 |
YBR103W |
SIR4-interacting protein SIF2; WD40 repeat-containing subunit of Set3C histone deacetylase complex; complex represses early/middle sporulation genes; antagonizes telomeric silencing; binds specificy to the Sir4p N-terminus |
| YMC2 |
YBR104W |
Carrier protein YMC2, mitochondrial; Putative mitochondrial inner membrane transporter; proposed role in oleate metabolism and glutamate biosynthesis; member of the mitochondrial carrier (MCF) family; YMC2 has a paralog, YMC1, that arose from the whole genome duplication |
| VID24 |
YBR105C |
Vacuolar import and degradation protein 24; GID Complex regulatory subunit; binds GID Complex in response to glucose through interactions with complex member Vid28p; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; promotes proteasome-dependent catabolite degradation of FBPase; peripheral membrane protein located at Vid (vacuole import and degradation) vesicles; To yeast YGR066c and S.pombe SpAC3H1.14 |
| SND3 |
YBR106W |
Protein involved in SRP-independent targeting of substrates to the ER; component of an alternative ER targeting pathway that has partial functional redundancy with the GET pathway; preference for substrates with downstream transmembrane domains; interacts with Snd1p, Env10p/Snd2p, and Sec61p-translocon subunits; can compensate for loss of SRP; role in phosphate transport, interacting with pho88, and in the maturation of secretory proteins |
| IML3 |
YBR107C |
Central kinetochore subunit IML3; Outer kinetochore protein and component of the Ctf19 complex; involved in the establishment of pericentromeric cohesion during mitosis; prevents non-disjunction of sister chromatids during meiosis II; forms a stable complex with Chl4p; required for localization of Sgo1p to pericentric sites during meiosis I; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-L and fission yeast fta1 |
| AIM3 |
YBR108W |
Altered inheritance of mitochondria protein 3; Protein that inhibits barbed-end actin filament elongation; interacts with Rvs167p; null mutant is viable and displays elevated frequency of mitochondrial genome loss; Belongs to the AIM3 family |
| CMD1 |
YBR109C |
Calmodulin; Ca2+ binding protein that regulates Ca2+ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca2+ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin; binds to the Hog1p MAPK in response to hyperosmotic stress; potentiates membrane tubulation and constriction mediated by the Rvs161p-Rvs167p complex; human CALM1 or CALM2 functiony complement repression induced inviability |
| ALG1 |
YBR110W |
Chitobiosyldiphosphodolichol beta-mannosyltransferase; Mannosyltransferase; involved in asparagine-linked glycosylation in the endoplasmic reticulum (ER); essential for viability; human homolog ALG1 complements yeast null mutant |
| YSA1 |
YBR111C |
Nudix hydrolase family member with ADP-ribose pyrophosphatase activity; shown to metabolize O-acetyl-ADP-ribose to AMP and acetylated ribose 5'-phosphate; Belongs to the Nudix hydrolase family. NudF subfamily |
| SUS1 |
YBR111W-A |
Transcription and mRNA export factor SUS1; Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP |
| CYC8 |
YBR112C |
General transcriptional co-repressor; acts together with Tup1p; also acts as part of a transcriptional co-activator complex that recruits the SWI/SNF and SAGA complexes to promoters; can form the prion [OCT+] |
| RAD16 |
YBR114W |
Nucleotide excision repair (NER) protein; binds damaged DNA during NER; binds DNA in an ATP-dependent manner (with Rad7p) during NER; required for NER of non-transcribed chromatin; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex |
| LYS2 |
YBR115C |
L-aminoadipate-semialdehyde dehydrogenase; Alpha aminoadipate reductase; catalyzes the reduction of alpha-aminoadipate to alpha-aminoadipate 6-semialdehyde, which is the fifth step in biosynthesis of lysine; activation requires posttranslational phosphopantetheinylation by Lys5p |
| TKL2 |
YBR117C |
Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL2 has a paralog, TKL1, that arose from the whole genome duplication |
| TEF2 |
YBR118W |
Translational elongation factor EF-1 alpha; in the GTP-bound active form, binds to and delivers aminoacylated tRNA to the A-site of ribosomes for elongation of nascent polypeptides; associates with vacuolar Rho1p GTPase; TEF2-RFP levels increase during replicative aging; may also have a role in tRNA re-export from the nucleus; TEF2 has a paralog, TEF1, that arose from the whole genome duplication |
| MUD1 |
YBR119W |
U1 snRNP A protein; homolog of human U1-A; involved in nuclear mRNA splicing; Belongs to the RRM U1 A/B'' family |
| CBP6 |
YBR120C |
Cytochrome B pre-mRNA-processing protein 6; Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex; Cbp3p-Cbp6p complex is sequestered if assembly of Complex III is blocked, downregulating COB mRNA translation |
| GRS1 |
YBR121C |
Glycine--tRNA ligase 1, mitochondrial; Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication; human homolog GARS implicated in Charcot-Marie-Tooth disease, can complement yeast null mutant |
| MRPL36 |
YBR122C |
Mitochondrial 54s ribosomal protein yml36; Mitochondrial ribosomal protein of the large subunit; overproduction suppresses mutations in the COX2 leader peptide-encoding region |
| TFC1 |
YBR123C |
Subunit of RNA polymerase III transcription initiation factor complex; one of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 |
| PTC4 |
YBR125C |
Cytoplasmic type 2C protein phosphatase (PP2C); identified as a high-copy number suppressor of cnb1 mpk1 synthetic lethality; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity |
| TPS1 |
YBR126C |
Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 56 kDa subunit; Synthase subunit of trehalose-6-P synthase/phosphatase complex; synthesizes the storage carbohydrate trehalose, which is criticy important for survival of long-term desiccation; also found in a monomeric form; expression is induced by the stress response and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid |
| MEO1 |
YBR126W-A |
Uncharacterized protein YBR126W-A; Putative protein of unknown function; identified by gene-trapping, microarray analysis, and genome-wide homology searches; mRNA identified as translated by ribosome profiling data; SWAT-GFP, seamless-GFP and mCherry fusion proteins localize to the endoplasmic reticulum; partiy overlaps the dubious ORF YBR126W-B |
| VMA2 |
YBR127C |
V-type proton ATPase subunit B; Subunit B of V1 peripheral membrane domain of vacuolar H+-ATPase; electrogenic proton pump found throughout the endomembrane system; contains nucleotide binding sites; also detected in the cytoplasm; protein abundance increases in response to DNA replication stress; human homolog ATP6V1B1, implicated in autosomal-recessive distal renal tubular acidosis (RTA) with sensorineural deafness, complements yeast null mutant; Belongs to the ATPase alpha/beta chains family |
| ATG14 |
YBR128C |
Autophagy-related protein 14; Autophagy-specific subunit of phosphatidylinositol 3-kinase complex I; Atg14p targets complex I to the phagophore assembly site (PAS); required for localizing additional ATG proteins to the PAS; required for overflow degradation of misfolded proteins when ERAD is saturated; homolog of human Barkor; other members are Vps34, Vps15, and Vps30p |
| OPY1 |
YBR129C |
Protein of unknown function; overproduction blocks cell cycle arrest in the presence of mating pheromone; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies |
| SHE3 |
YBR130C |
SWI5-dependent HO expression protein 3; Protein adaptor between Myo4p and the She2p-mRNA complex; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud; also required for cortical ER inheritance |
| CCZ1 |
YBR131W |
Vacuolar fusion protein CCZ1; Subunit of a heterodimeric guanine nucleotide exchange factor (GEF); subunit of the Mon1-Ccz1 GEF complex, which stimulates nucleotide exchange and activation of Ypt7p, a Rab family GTPase involved in membrane tethering and fusion events at the late endosome and vacuole; GEF activity is stimulated by membrane association and anionic phospholipids; involved in localizing Ypt7p to the vacuolar membrane; required for macroautophagy, the CVT pathway and mitophagy |
| AGP2 |
YBR132C |
General amino acid permease AGP2; Plasma membrane regulator of polyamine and carnitine transport; has similarity to transporters but lacks transport activity; may act as a sensor that transduces environmental signals; has a positive or negative regulatory effect on transcription of many transporter genes |
| HSL7 |
YBR133C |
Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent localization to the bud neck in budded cells and periodic Hsl1p-dependent phosphorylation; required with Hsl1p, and Elm1p for the mother-bud neck recruitment, phosphorylation, and degradation of Swe1p; interacts directly with Swe1p; relocalizes away from bud neck upon DNA replication stress; human homolog PRMT5 can complement yeast hsl7 mutant |
| CKS1 |
YBR135W |
Cyclin-dependent protein kinase regulatory subunit and adaptor; interacts with Cdc28p (aka Cdk1p); required for G1/S and G2/M phase transitions and budding; mediates phosphorylation and degradation of Sic1p; modulates proteolysis of M-phase targets through interactions with the proteasome; role in transcriptional regulation, recruiting proteasomal subunits to target gene promoters; human homologs CKS1B and CKS2 can each complement yeast cks1 null mutant |
| MEC1 |
YBR136W |
Serine/threonine-protein kinase MEC1; Genome integrity checkpoint protein and PI kinase superfamily member; Mec1p and Dun1p function in same pathway to regulate dNTP pools and telomere length; signal transducer required for cell cycle arrest and transcriptional responses to damaged or unreplicated DNA; facilitates replication fork progression and regulates P-body formation under replication stress; promotes interhomolog recombination by phosphorylating Hop1p; associates with shortened, dysfunctional telomeres; Belongs to the PI3/PI4-kinase family. ATM subfamily |
| YBR137W |
YBR137W |
UPF0303 protein YBR137W; Protein with a role in ER delivery of tail-anchored membrane proteins; interacts with Sgt2p; binds to the TRC complex, which inserts proteins into the ER membrane; interacts with Msn5p karyopherin; YBR137W is not an essential gene; Belongs to the UPF0303 family |
| YBR138C |
YBR138C |
Uncharacterized protein YBR138C; Cytoplasmic protein of unknown function; APCC(Cdh1) substrate; potentiy phosphorylated by Cdc28p; YBR138C is not an essential gene |
| ATG42 |
YBR139W |
Putative serine type carboxypeptidase; role in phytochelatin synthesis; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner |
| IRA1 |
YBR140C |
Inhibitory regulator protein IRA1; GTPase-activating protein; negatively regulates RAS by converting it from GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; mutations cause catalase T deficiency, defective glycogen synthesis and defective trehalose accumulation; IRA1 has a paralog, IRA2, that arose from the whole genome duplication; defects in human homolog NF1 are associated with neurofibromatosis |
| BMT2 |
YBR141C |
25S rRNA (adenine(2142)-N(1))-methyltransferase; Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 2142; belongs to Rossmann fold superfamily; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene |
| MAK5 |
YBR142W |
Essential nucleolar protein; putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits; Belongs to the DEAD box helicase family. DDX24/MAK5 subfamily |
| SUP45 |
YBR143C |
Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p |
| YBR144C |
YBR144C |
Uncharacterized protein YBR144C; Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YBR144C is not an essential gene |
| ADH5 |
YBR145W |
Alcohol dehydrogenase isoenzyme V; involved in ethanol production; ADH5 has a paralog, ADH1, that arose from the whole genome duplication; Belongs to the zinc-containing alcohol dehydrogenase family |
| MRPS9 |
YBR146W |
Mitochondrial 37s ribosomal protein mrps9; Mitochondrial ribosomal protein of the sm subunit |
| RTC2 |
YBR147W |
Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; positive regulation by Lys14p suggests that lysine may be the primary substrate; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter; RTC2 has a paralog, YPQ1, that arose from the whole genome duplication; Belongs to the laat-1 family |
| YSW1 |
YBR148W |
Spore-specific protein YSW1; Protein required for normal prospore membrane formation; interacts with Gip1p, which is the meiosis-specific regulatory subunit of the Glc7p protein phosphatase; expressed specificy in spores and localizes to the prospore membrane; YSW1 has a paralog, SPO21, that arose from the whole genome duplication |
| ARA1 |
YBR149W |
D-arabinose dehydrogenase [NAD(P)+] heavy chain; NADP+ dependent arabinose dehydrogenase; involved in carbohydrate metabolism; purified as homodimer; natury occurs with a N-terminus degradation product; Belongs to the aldo/keto reductase family |
| TBS1 |
YBR150C |
Uncharacterized transcriptional regulatory protein TBS1; Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; TBS1 has a paralog, HAL9, that arose from the whole genome duplication |
| APD1 |
YBR151W |
Actin patches distal protein 1; Protein of unknown function; required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; localizes to both cytoplasm and nucleus; Belongs to the APD1 family |
| SPP381 |
YBR152W |
Pre-mRNA-splicing factor SPP381; mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts geneticy and physicy with Prp38p; relocalizes to the cytosol in response to hypoxia; Belongs to the SPP381 family |
| RIB7 |
YBR153W |
2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate reductase; Diaminohydroxyphoshoribosylaminopyrimidine deaminase; catalyzes the second step of the riboflavin biosynthesis pathway |
| RPB5 |
YBR154C |
RNA polymerase subunit ABC27; common to RNA polymerases I, II, and III; contacts DNA and affects transactivation; Belongs to the archaeal RpoH/eukaryotic RPB5 RNA polymerase subunit family |
| CNS1 |
YBR155W |
Hsp70/Hsp90 co-chaperone CNS1; TPR-containing co-chaperone; binds both Hsp82p (Hsp90) and Ssa1p (Hsp70); stimulates ATPase activity of Ssa1p; ts mutants reduce Hsp82p function, overexpression suppresses phenotypes of HSP82 ts ele and cpr7 deletion; human homolog TTC4 complements yeast cns1 mutant; Belongs to the TTC4 family |
| SLI15 |
YBR156C |
Inner centromere protein-related protein SLI15; Subunit of the conserved chromosomal passenger complex (CPC); complex regulates kinetochore-microtubule attachments, activation of the spindle tension checkpoint, and mitotic spindle disassembly; other complex members are Ipl1p, Bir1p, and Nbl1p |
| ICS2 |
YBR157C |
Increased copper sensitivity protein 2; Protein of unknown function; null mutation does not confer any obvious defects in growth, spore germination, viability, or carbohydrate utilization |
| AMN1 |
YBR158W |
Antagonist of mitotic exit network protein 1; Protein required for daughter cell separation; multiple mitotic checkpoints, and chromosome stability; contains 12 degenerate leucine-rich repeat motifs; expression is induced by the Mitotic Exit Network (MEN); Belongs to the AMN1 family |
| IFA38 |
YBR159W |
Very-long-chain 3-oxoacyl-CoA reductase; Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides; Belongs to the short-chain dehydrogenases/reductases (SDR) family |
| CDC28 |
YBR160W |
Cyclin-dependent kinase (CDK) catalytic subunit; master regulator of mitotic and meiotic cell cycles; alternately associates with G1, S, G2/M phase cyclins, which provide substrate specificity; regulates metabolism, basal transcription, chromosome dynamics, growth and morphogenesis; transcript induction in osmostress involves antisense RNA; human homologs CDK1, CDK2, CDK3 can complement yeast conditional cdc28 mutants; human CDK1, CDK2 can complement yeast cdc28 null mutant; Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily |
| CSH1 |
YBR161W |
Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication |
| TOS1 |
YBR162C |
Protein TOS1; Covalently-bound cell w protein of unknown function; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C; Belongs to the PGA52 family |
| YSY6 |
YBR162W-A |
Protein of unknown function; expression suppresses a secretory pathway mutation in E. coli; has similarity to the mammalian RAMP4 protein involved in secretion |
| EXO5 |
YBR163W |
Mitochondrial 5'-3' exonuclease and sliding exonuclease; required for mitochondrial genome maintenance; distantly related to the RecB nuclease domain of bacterial RecBCD recombinases; may be regulated by the transcription factor Ace2 |
| ARL1 |
YBR164C |
ADP-ribosylation factor-like protein 1; Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; role in membrane organization at trans-Golgi network; required for delivery of Atg9p to the phagophore assembly site during autophagy under high temperature stress; required with Ypt6p for starvation-induced autophagy; required for the CVT pathway under non-starvation conditions; G protein of the Ras superfamily, similar to ADP-ribosylation factor |
| UBS1 |
YBR165W |
Ubiquitin-conjugating enzyme suppressor that regulates Cdc34p; functions as a general positive regulator of Cdc34p activity; nuclear protein that may represent a link between nucleocytoplasmic transport and ubiquitin ligase activity |
| TYR1 |
YBR166C |
Prephenate dehydrogenase involved in tyrosine biosynthesis; expression is dependent on phenylalanine levels; Belongs to the prephenate/arogenate dehydrogenase family |
| POP7 |
YBR167C |
Ribonucleases P/MRP protein subunit POP7; Subunit of RNase MRP, nuclear RNase P and telomerase; forms a soluble heterodimer with Pop6p that binds P3 domain of RNase MRP and RNase P RNAs; RNase MRP cleaves pre-rRNA, nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs, while telomerase replenishes telomeric DNA |
| PEX32 |
YBR168W |
Peroxisomal integral membrane protein; involved in negative regulation of peroxisome size; partiy functiony redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p |
| SSE2 |
YBR169C |
Member of Hsp110 subclass of the heat shock protein 70 (HSP70) family; serves as nucleotide exchange factor to load ATP onto the SSA class of cytosolic Hsp70s; may be involved in protein folding; localized to the cytoplasm; SSE2 has a paralog, SSE1, that arose from the whole genome duplication |
| NPL4 |
YBR170C |
Nuclear protein localization protein 4; Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; assists Cdc48p in the dislocation of misfolded, polyubiquitinated ERAD substrates that are subsequently delivered to the proteasome for degradation; also involved in the regulated destruction of resident ER membrane proteins, such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing (RUP) |
| SEC66 |
YBR171W |
Translocation protein SEC66; Non-essential subunit of Sec63 complex; with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER; other members are Sec63p, Sec62p, and Sec72p |
| SMY2 |
YBR172C |
GYF domain protein; involved in COPII vesicle formation; interacts with the Sec23p/Sec24p subcomplex; overexpression suppresses the temperature sensitivity of a myo2 mutant; similar to S. pombe Mpd2; SMY2 has a paralog, SYH1, that arose from the whole genome duplication; Belongs to the SMY2/mpd2 family |
| UMP1 |
YBR173C |
Chaperone required for correct maturation of the 20S proteasome; short-lived chaperone; may inhibit premature dimerization of proteasome half-mers; degraded by proteasome upon completion of its assembly |
| SWD3 |
YBR175W |
COMPASS component SWD3; Essential subunit of the COMPASS (Set1C) complex; COMPASS methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member and ortholog of mammalian WDR5 |
| ECM31 |
YBR176W |
3-methyl-2-oxobutanoate hydroxymethyltransferase; Ketopantoate hydroxymethyltransferase; required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2-dehydropantoate |
| EHT1 |
YBR177C |
Acyl-coenzymeA:ethanol O-acyltransferase; plays a minor role in medium-chain fatty acid ethyl ester biosynthesis; possesses short-chain esterase activity; localizes to lipid particles and the mitochondrial outer membrane; EHT1 has a paralog, EEB1, that arose from the whole genome duplication |
| FZO1 |
YBR179C |
Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system; Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. Mitofusin subfamily |
| DTR1 |
YBR180W |
Putative dityrosine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for spore w synthesis; sequence similarity to QDR1 and QDR3, and the triple mutant dtr1 qdr1 qdr3 exhibits reduced dityrosine fluorescence relative to the single mutants; expressed during sporulation |
| RPS6B |
YBR181C |
Protein component of the sm (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; phosphorylated on S233 by Ypk3p in a TORC1-dependent manner, and on S232 in a TORC1/2-dependent manner by Ypk1/2/3p; RPS6B has a paralog, RPS6A, that arose from the whole genome duplication |
| SMP1 |
YBR182C |
MADS-box transcription factor involved in osmotic stress response; SMP1 has a paralog, RLM1, that arose from the whole genome duplication; Belongs to the MEF2 family |
| YBR182C-A |
YBR182C-A |
Uncharacterized protein YBR182C-A; Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching |
| YPC1 |
YBR183W |
Alkaline ceramidase; also has reverse (CoA-independent) ceramide synthase activity; catalyzes both breakdown and synthesis of phytoceramide; overexpression confers fumonisin B1 resistance; YPC1 has a paralog, YDC1, that arose from the whole genome duplication |
| YBR184W |
YBR184W |
Uncharacterized protein ybr184w; Putative protein of unknown function; YBR184W is not an essential gene |
| MBA1 |
YBR185C |
Membrane-associated mitochondrial ribosome receptor; forms a complex with Mdm38p that may facilitate recruitment of mRNA-specific translational activators to ribosomes; possible role in protein export from the matrix to inner membrane |
| PCH2 |
YBR186W |
Pachytene checkpoint protein 2; Hexameric ring ATPase that remodels chromosome axis protein Hop1p; nucleolar component of the pachytene checkpoint, which prevents chromosome segregation when recombination and chromosome synapsis are defective; also represses meiotic interhomolog recombination in rDNA; required for meiotic double-stranded break formation |
| GDT1 |
YBR187W |
GCR1-dependent translation factor 1; Calcium transporter localized to the cis- and medial-Golgi apparatus; required for protein glycosylation; GFP-fusion protein localizes to the vacuole; TMEM165, a human gene which causes Congenital Disorders of Glycosylation is orthologous and functiony complements the null ele; expression pattern and physical interactions suggest a possible role in ribosome biogenesis; expression reduced in a gcr1 null mutant; Belongs to the GDT1 family |
| NTC20 |
YBR188C |
Pre-mRNA-splicing factor NTC20; Member of the NineTeen Complex (NTC); this complex contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs |
| RPS9B |
YBR189W |
Protein component of the sm (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9B has a paralog, RPS9A, that arose from the whole genome duplication |
| RPL21A |
YBR191W |
Ribosomal 60S subunit protein L21A; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21A has a paralog, RPL21B, that arose from the whole genome duplication |
| RIM2 |
YBR192W |
Mitochondrial carrier protein RIM2; Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family |
| MED8 |
YBR193C |
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation |
| AIM4 |
YBR194W |
Altered inheritance of mitochondria protein 4; Protein proposed to be associated with the nuclear pore complex; null mutant is viable, displays elevated frequency of mitochondrial genome loss and is sensitive to freeze-thaw stress; Belongs to the AIM4 family |
| MSI1 |
YBR195C |
Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 affects multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of DNA damage checkpoint after DNA repair; chromatin dynamics during transcription; and repression of divergent noncoding transcription; Msi1p localizes to nucleus and cytoplasm and independently regulates the RAS/cAMP pathway via sequestration of Npr1p kinase |
| PGI1 |
YBR196C |
Glycolytic enzyme phosphoglucose isomerase; catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation |
| YBR196C-A |
YBR196C-A |
Uncharacterized protein YBR196C-A; Putative protein of unknown function; identified by fungal homology and RT-PCR |
| YBR196C-B |
YBR196C-B |
Uncharacterized protein YBR196C-B; Putative protein of unknown function; identified by expression profiling and mass spectrometry |
| YBR197C |
YBR197C |
Uncharacterized protein YBR197C; Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress; YBR197C has a paralog, YPL077C, that arose from the whole genome duplication |
| TAF5 |
YBR198C |
Transcription initiation factor tfiid subunit 5; Subunit (90 kDa) of TFIID and SAGA complexes; involved in RNA polymerase II transcription initiation and in chromatin modification |
| KTR4 |
YBR199W |
Probable mannosyltransferase KTR4; Glycosyltransferase involved in protein glycosylation; transfers GDP-mannose to methyl-alpha-mannoside in vitro; member of the KRE2/MNT1 mannosyltransferase family of type II membrane proteins with a short cytoplasmic N-terminus, a membrane-spanning region and a highly conserved catalytic lumenal domain |
| BEM1 |
YBR200W |
Bud emergence protein 1; Protein containing SH3-domains; involved in establishing cell polarity and morphogenesis; functions as a scaffold protein for complexes that include Cdc24p, Ste5p, Ste20p, and Rsr1p |
| YBR200W-A |
YBR200W-A |
Uncharacterized protein YBR200W-A; Putative protein of unknown function; identified by fungal homology and RT-PCR |
| DER1 |
YBR201W |
Degradation in the endoplasmic reticulum protein 1; ER membrane protein that promotes export of misfolded polypeptides; required for ER-associated protein degradation of misfolded or unassembled proteins; initiates export of aberrant polypeptides from ER lumen by threading them into ER membrane and routing them to Hrd1p for ubiquitination; function normy requires N-terminal acetylation by NatB; N- and C- termini protrude into cytoplasm; similar to Dfm1p; homolog of mammalian derlin-1 |
| MIN7 |
YBR201C-A |
Putative uncharacterized protein ybr201c-a; Putative protein of unknown function |
| MCM7 |
YBR202W |
DNA replication licensing factor MCM7; Component of the Mcm2-7 hexameric helicase complex; MCM2-7 primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex |
| COS111 |
YBR203W |
F-box protein COS111; Protein required for antifungal drug ciclopirox olamine resistance; not related to the subtelomericy-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies |
| LDH1 |
YBR204C |
Lipid droplet hydrolase 1; Serine hydrolase; exhibits active esterase plus weak triacylglycerol lipase activities; proposed role in lipid homeostasis, regulating phospholipid and non-polar lipid levels and required for mobilization of LD-stored lipids; localizes to the lipid droplet (LD) surface; contains a classical serine containing catalytic triad (GxSxG motif) |
| KTR3 |
YBR205W |
Probable mannosyltransferase KTR3; Putative alpha-1,2-mannosyltransferase; involved in O- and N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; Svp26p mediates uptake of Ktr3p into COPII vesicles; relocalizes from nucleus to vacuole upon DNA replication stress; Belongs to the glycosyltransferase 15 family |
| FTH1 |
YBR207W |
Putative high affinity iron transporter; involved in transport of intravacuolar stores of iron; forms complex with Fet5p; expression is regulated by iron; proposed to play indirect role in endocytosis; protein abundance increases in response to DNA replication stress |
| DUR1,2 |
YBR208C |
Urea amidolyase; contains both urea carboxylase and ophanate hydrolase activities, degrades urea to CO2 and NH3; expression sensitive to nitrogen catabolite repression and induced by ophanate, an intermediate in antoin degradation; protein abundance increases in response to DNA replication stress |
| YBR209W |
YBR209W |
Uncharacterized protein YBR209W; Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YBR209W is not an essential gene |
| YNCB0017C |
YNCB0017C |
Unknown |
| ERV15 |
YBR210W |
ER-derived vesicles protein ERV15; Protein involved in export of proteins from the endoplasmic reticulum; ERV15 has a paralog, ERV14, that arose from the whole genome duplication |
| AME1 |
YBR211C |
Central kinetochore subunit AME1; Essential kinetochore protein associated with microtubules and SPBs; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-U and fission yeast Mis17; relative distribution to the nucleus increases upon DNA replication stress |
| NGR1 |
YBR212W |
RNA binding protein that negatively regulates growth rate; interacts with the 3' UTR of the mitochondrial porin (POR1) mRNA and enhances its degradation; overexpression impairs mitochondrial function; interacts with Dhh1p to mediate POR1 mRNA decay; expressed in stationary phase |
| MET8 |
YBR213W |
Siroheme biosynthesis protein MET8; Bifunctional dehydrogenase and ferrochelatase; involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis; Belongs to the precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase family. MET8 subfamily |
| SDS24 |
YBR214W |
Protein involved in cell separation during budding; one of two S. cerevisiae homologs (Sds23p and Sds24p) of the S. pombe Sds23 protein, which is implicated in APC/cyclosome regulation; may play an indirect role in fluid-phase endocytosis; protein abundance increases in response to DNA replication stress; SDS24 has a paralog, SDS23, that arose from the whole genome duplication |
| HPC2 |
YBR215W |
Histone promoter control protein 2; Subunit of the HIR complex; HIR is a nucleosome assembly complex involved in regulation of histone gene transcription; mutants display synthetic defects with subunits of FACT, a complex that ows passage of RNA Pol II through nucleosomes |
| YBP1 |
YBR216C |
YAP1-binding protein 1; Protein involved in cellular response to oxidative stress; required for oxidation of specific cysteine residues of transcription factor Yap1p, resulting in nuclear localization of Yap1p in response to stress; YBP1 has a paralog, YBP2, that arose from the whole genome duplication |
| ATG12 |
YBR217W |
Ubiquitin-like protein ATG12; Ubiquitin-like modifier involved in autophagy and the Cvt pathway; conserved; conjugated to Atg5p to form a complex involved in Atg8p lipidation; Atg5p-Atg12p conjugate enhances E2 activity of Atg3p by rearranging its catalytic site, also forms a complex with Atg16p; the Atg5-Atg12/Atg16 complex binds to membranes and is essential for autophagosome formation |
| PYC2 |
YBR218C |
Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentiy regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication |
| YBR219C |
YBR219C |
Uncharacterized protein ybr219c; Putative protein of unknown function; YBR219C is not an essential gene |
| YBR220C |
YBR220C |
Uncharacterized membrane protein ybr220c; Putative protein of unknown function; YBR220C is not an essential gene |
| PDB1 |
YBR221C |
Pyruvate dehydrogenase e1 component subunit beta, mitochondrial; E1 beta subunit of the pyruvate dehydrogenase (PDH) complex; PDH is an evolutionarily conserved multi-protein complex found in mitochondria |
| YBR221W-A |
YBR221W-A |
Uncharacterized protein YBR221W-A; Putative protein of unknown function; identified by expression profiling and mass spectrometry |
| PCS60 |
YBR222C |
Oxalyl-CoA synthetase; capable of catalyzing conversion of oxalate to oxalyl-CoA; catalyzes first step in pathway of oxalate degradation that functions to protect yeast from inhibitory effects of oxalate; peroxisomal protein that binds mRNA; localizes to both peroxisomal peripheral membrane and matrix, expression is highly inducible by oleic acid; similar to E. coli long chain acyl-CoA synthetase; Belongs to the ATP-dependent AMP-binding enzyme family |
| TDP1 |
YBR223C |
Tyrosyl-DNA phosphodiesterase I; hydrolyzes 3' and 5'-phosphotyrosyl bonds; involved in the repair of DNA lesions created by topo I and topo II; mutations in the human homolog, TDP1, result in the a neurodegenerative disease, spinocerebellar ataxia with axonal neuropathy (SCAN1); yeast cells and human rhabdomyosarcoma lines that overexpress TDP1 both exhibit elevated dosage chromosomal instability (dCIN) |
| YBR225W |
YBR225W |
Uncharacterized protein YBR225W; Putative protein of unknown function; non-essential gene identified in a screen for mutants affected in mannosylphophorylation of cell w components |
| MCX1 |
YBR227C |
ATP-dependent clpX-like chaperone, mitochondrial; Non-proteolytic ATPase of the AAA family; stimulates incorporation of the pyridoxal phosphate cofactor into Hem1p (5-aminolevulinic acid synthase); localized to the mitochondrial matrix; ortholog of vertebrate CLPX, which promotes erythropoiesis |
| SLX1 |
YBR228W |
Endonuclease involved in DNA recombination and repair; subunit of a complex, with Slx4p, that hydrolyzes 5' branches from duplex DNA in response to sted or converging replication forks; function overlaps with that of Sgs1p-Top3p |
| ROT2 |
YBR229C |
Mannosyl-oligosaccharide alpha-1,3-glucosidase; Glucosidase II catalytic subunit; required to trim the final glucose in N-linked glycans; required for normal cell w synthesis; mutations in rot2 suppress tor2 mutations, and are syntheticy lethal with rot1 mutations |
| OM14 |
YBR230C |
Mitochondrial outer membrane receptor for cytosolic ribosomes; integral protein of the outer membrane that interacts with the nascent chain-associated complex (NAC) bound to ribosomes, contributing to co-translational mitochondrial import; interacts with porin (Por1p) and Om45p; abundance is decreased in cells grown in glucose relative to other carbon sources |
| COQ21 |
YBR230W-A |
Putative uncharacterized protein ybr230w-a; Putative protein of unknown function; YBR230W-A has a paralog, COQ8, that arose from the whole genome duplication |
| LSR1 |
YNCB0019C |
Unknown |
| SWC5 |
YBR231C |
SWR1-complex protein 5; Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress; relocalizes to the cytosol in response to hypoxia; Belongs to the SWC5 family |
| PBP2 |
YBR233W |
PAB1-binding protein 2; RNA binding protein; has similarity to mammalian heterogeneous nuclear RNP K protein, involved in the regulation of telomere position effect and telomere length; relative distribution to the nucleus increases upon DNA replication stress |
| DAD3 |
YBR233W-A |
Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis |
| ARC40 |
YBR234C |
Subunit of the ARP2/3 complex; ARP2/3 is required for the motility and integrity of cortical actin patches; Belongs to the WD repeat ARPC1 family |
| VHC1 |
YBR235W |
Solute carrier family 12 (potassium/chloride transporters), member 9; Vacuolar membrane cation-chloride cotransporter (CCC); likely mediates K+ and Cl- cotransport into the vacuole; has a role in potassium homeostasis and salt tolerance; localizes to sites of contact between the vacuole and mitochondria (vCLAMPs); similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family |
| ABD1 |
YBR236C |
mRNA cap guanine-N7 methyltransferase; Methyltransferase; catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA; nuclear protein that relocalizes to the cytosol in response to hypoxia |
| PRP5 |
YBR237W |
Pre-mRNA-processing ATP-dependent RNA helicase PRP5; RNA helicase in the DEAD-box family; necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA; Belongs to the DEAD box helicase family. DDX46/PRP5 subfamily |
| YBR238C |
YBR238C |
Mitochondrial membrane protein; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptiony up-regulated by TOR; deletion increases life span; YBR238C has a paralog, RMD9, that arose from the whole genome duplication |
| ERT1 |
YBR239C |
Transcriptional regulator; involved in regulation of gluconeogenesis and fermentable carbon utilization; GFP-fusion protein localizes to cytoplasm, nucleus; null mutation affects periodicity of transcriptional and metabolic oscillation; plays role in restricting Ty1 transposition; member of the zinc cluster family of proteins, similar to Rds2p |
| THI2 |
YBR240C |
Thiamine biosynthesis regulatory protein; Transcriptional activator of thiamine biosynthetic genes; interacts with regulatory factor Thi3p to control expression of thiamine biosynthetic genes with respect to thiamine availability; acts together with Pdc2p to respond to thiaminediphosphate demand, possibly as related to carbon source availability; zinc finger protein of the Zn(II)2Cys6 type |
| VVS1 |
YBR241C |
Probable metabolite transport protein YBR241C; Putative transporter, member of the sugar porter family; green fluorescent protein (GFP)-fusion protein localizes to the vacuolar membrane; YBR241C is not an essential gene; YBR241C has a paralog, VPS73, that arose from the whole genome duplication |
| YBR242W |
YBR242W |
HD domain-containing protein YBR242W; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene; YBR242W has a paralog, YGL101W, that arose from the whole genome duplication |
| ALG7 |
YBR243C |
UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase; UDP-N-acetyl-glucosamine-1-P transferase; transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin; human homolog DPAGT1 can complement yeast ALG7 mutant |
| GPX2 |
YBR244W |
Glutathione peroxidase-like peroxiredoxin 2; Phospholipid hydroperoxide glutathione peroxidase; protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress; induced by glucose starvation; protein abundance increases in response to DNA replication stress |
| ISW1 |
YBR245C |
ISWI chromatin-remodeling complex ATPase ISW1; ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; with Ioc3p forms Isw1a complex involved in repression of transcription initiation; with Ioc2p and Ioc4p forms Isw1b complex involved in regulation of transcription elongation; Isw1b recruited to ORFs by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions; Isw1p import into nucleus depends on C-terminal bipartite nuclear targeting signal KRIR X19 KKAK |
| RRT2 |
YBR246W |
Diphthine methyltransferase; Methylesterase performing penultimate step of diphthamide biosynthesis; hydrolyzes methylated diphthine to produce diphthine and ows Dph6-catalyzed amidation reaction to occur; deletion leads to resistance to sordarin and accumulation of methylatediphthine; WD40 domain-containing protein; involved in endosomal recycling; forms complex with Rtt10p that functions in retromer-mediated pathway for recycling internalized cell-surface proteins |
| ENP1 |
YBR247C |
Protein associated with U3 and U14 snoRNAs; required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus; human BYSL functiony complements the heat sensitivity of an enp1 ts mutant; Belongs to the bystin family |
| HIS7 |
YBR248C |
Imidazole glycerol phosphate synthase; glutamine amidotransferase:cyclase that catalyzes the fifth step of histidine biosynthesis and also produces 5-aminoimidazole-4-carboxamide ribotide (AICAR), a purine precursor; In the C-terminal section; belongs to the HisA/HisF family |
| ARO4 |
YBR249C |
Phospho-2-dehydro-3-deoxyheptonate aldolase, tyrosine-inhibited; 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase; catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by tyrosine or high concentrations of phenylalanine or tryptophan; relative distribution to the nucleus increases upon DNA replication stress |
| SPO23 |
YBR250W |
Sporulation protein 23; Protein of unknown function; associates with meiosis-specific protein Spo1p |
| MRPS5 |
YBR251W |
Mitochondrial 37s ribosomal protein mrps5; Mitochondrial ribosomal protein of the sm subunit |
| DUT1 |
YBR252W |
Deoxyuridine 5'-triphosphate nucleotidohydrolase; Deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate; human homolog DUT ows growth of yeast haploid dut1 null mutant after sporulation of heterozygous diploid |
| SRB6 |
YBR253W |
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation |
| TRS20 |
YBR254C |
Core component of transport protein particle (TRAPP) complexes I-III; TRAPPs are multimeric guanine nucleotide-exchange factors for GTPase Ypt1p, regulating ER-Golgi traffic (TRAPPI), intra-Golgi traffic (TRAPPII), endosome-Golgi traffic (TRAPPII and III) and autophagy (TRAPPIII); mutation leads to defects in endocytic recycling, block in sporulation/meiosis; mutations in human homolog TRAPPC2 cause spondyloepiphyseal dysplasia tarda, TRAPPC2 can complement yeast null mutant |
| MTC4 |
YBR255W |
Maintenance of telomere capping protein 4; Protein of unknown function; required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is syntheticy sick with cdc13-1 |
| RCF3 |
YBR255C-A |
Uncharacterized protein YBR255C-A; Putative protein of unknown function; may interact with respiratory chain complexes III (ubiquinol-cytochrome c reductase) or IV (cytochrome c oxidase); identified by sequence comparison with hemiascomycetous yeast species |
| RIB5 |
YBR256C |
Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway |
| POP4 |
YBR257W |
RNases MRP/P 32.9 kDa subunit; Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P |
| SHG1 |
YBR258C |
COMPASS component SHG1; Subunit of the COMPASS (Set1C) complex; COMPASS methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres |
| YBR259W |
YBR259W |
Protein of unknown function; YBR259W is not an essential gene; forms cytoplasmic foci upon DNA replication stress |
| RGD1 |
YBR260C |
Rho gtpase-activating protein rgd1; GTPase-activating protein (RhoGAP) for Rho3p and Rho4p; possibly involved in control of actin cytoskeleton organization |
| TAE1 |
YBR261C |
Alpha N-terminal protein methyltransferase 1; AdoMet-dependent proline methyltransferase; catalyzes the dimethylation of ribosomal proteins Rpl12 and Rps25 at N-terminal proline residues; has a role in protein synthesis; fusion protein localizes to the cytoplasm; Belongs to the methyltransferase superfamily. NTM1 family |
| MIC12 |
YBR262C |
Component of the MICOS complex; MICOS (formerly MINOS or MitOS) is a mitochondrial inner membrane complex that extends into the intermembrane space and has a role in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane; forms a subcomplex with Mic10p and Mic27p whose assembly and stability requires cardiolipin |
| SHM1 |
YBR263W |
Mitochondrial serine hydroxymethyltransferase; converts serine to glycine plus 5,10 methylenetetrahydrofolate; involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis; reverse reaction generates serine; Belongs to the SHMT family |
| YPT10 |
YBR264C |
Rab family GTP-binding protein; contains the PEST signal sequence specific for proteolytic enzymes; may be involved in vesicular transport; overexpression leads to accumulation of Golgi-like cisternae with budding vesicles |
| TSC10 |
YBR265W |
3-ketodihydrosphingosine reductase TSC10; 3-ketosphinganine reductase; catalyzes the second step in phytosphingosine synthesis; essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine; localized to lipid droplets; member of short chain dehydrogenase/reductase protein family |
| REI1 |
YBR267W |
Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network |
| SLM6 |
YBR266C |
Protein slm6; Protein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase |
| MRPL37 |
YBR268W |
Mitochondrial 54s ribosomal protein yml37; Mitochondrial ribosomal protein of the large subunit |
| SDH8 |
YBR269C |
Protein required for assembly of succinate dehydrogenase; interacts with flavinylated Sdh1p and may function as a chaperone for free Sdh1p, protecting its FAD cofactor from redox reactions before assembly of the complex; soluble protein of the mitochondrial matrix; respiratory defect of null mutant is functiony complemented by Drosophila and human orthologs |
| BIT2 |
YBR270C |
Subunit of TORC2 membrane-associated complex; involved in regulation of actin cytoskeletal dynamics during polarized growth and cell w integrity; interacts with Slm1p and Slm2p, homologous PH domain-containing TORC2 substrates; BIT2 has a paralog, BIT61, that arose from the whole genome duplication |
| EFM2 |
YBR271W |
Protein-lysine N-methyltransferase EFM2; S-adenosylmethionine-dependent methyltransferase; seven-beta-strand lysine methyltransferase which dimethylates translation elongation factor EF2 (Eft1p and Eft2p) at lysine 613 and methylates EF3 (Yef3p) at lysine 187; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; involved in regulation of translational termination; predicted involvement in ribosome biogenesis; Belongs to the class I-like SAM-binding methyltransferase superfamily. METTL21 family |
| HSM3 |
YBR272C |
DNA mismatch repair protein HSM3; Evolutionarily conserved 19S regulatory particle assembly-chaperone; involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; structural study suggests Hsm3p is a scaffold protein for Rpt1p-Rpt2p complex formation; ortholog of human 19S subunit S5b |
| UBX7 |
YBR273C |
UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p; UBX7 has a paralog, UBX6, that arose from the whole genome duplication |
| CHK1 |
YBR274W |
Serine/threonine-protein kinase CHK1; Serine/threonine kinase and DNA damage checkpoint effector; mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase |
| RIF1 |
YBR275C |
Telomere length regulator protein RIF1; Protein that binds to the Rap1p C-terminus; acts synergisticy with Rif2p to help control telomere length and establish telomeric silencing; involved in control of DNA replication; contributes to resection of DNA double strand breaks (DSBs); deletion results in telomere elongation; Belongs to the RIF1 family |
| PPS1 |
YBR276C |
Protein phosphatase; has specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle |
| DPB3 |
YBR278W |
Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication |
| PAF1 |
YBR279W |
RNA polymerase II-associated protein 1; Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 |
| SAF1 |
YBR280C |
Scf ubiquitin ligase complex subunit saf1; SCF-associated factor 1; F-Box protein involved in proteasome-dependent degradation of Aah1p; involved in proteasome-dependent degradation of Aah1p during entry of cells into quiescence; interacts with Skp1 |
| DUG2 |
YBR281C |
Probable di- and tripeptidase DUG2; Component of glutamine amidotransferase (GATase II); forms a complex with Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) |
| MRPL27 |
YBR282W |
Mitochondrial 54s ribosomal protein yml27; Mitochondrial ribosomal protein of the large subunit; homolog of human Bcl-2 interacting protein BMRP |
| SSH1 |
YBR283C |
Subunit of the Ssh1 translocon complex; Sec61p homolog involved in co-translational pathway of protein translocation; not essential |
| YBR284W |
YBR284W |
Inactive deaminase YBR284W; Putative meto-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication |
| YBR285W |
YBR285W |
Putative uncharacterized protein ybr285w; Putative protein of unknown function; YBR285W is not an essential gene |
| APE3 |
YBR286W |
Vacuolar aminopeptidase Y; processed to mature form by Prb1p |
| YBR287W |
YBR287W |
Uncharacterized transporter YBR287W; Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER; YBR287W is not an essential gene; Belongs to the auxin efflux carrier (TC 2.A.69) family |
| APM3 |
YBR288C |
Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway |
| SNF5 |
YBR289W |
Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions |
| BSD2 |
YBR290W |
Heavy metal ion homeostasis protein; facilitates trafficking of Smf1p and Smf2p metal transporters to vacuole where they are degraded; acts as an adaptor protein with Rsp5p in the regulated endocytosis of Smf1p and is itself ubiquitylated by Rsp5p; controls metal ion transport, prevents metal hyperaccumulation, functions in copper detoxification; Belongs to the BSD2 family |
| CTP1 |
YBR291C |
Tricarboxylate transport protein; Mitochondrial inner membrane citrate transporter; member of the mitochondrial carrier family |
| YBR292C |
YBR292C |
Uncharacterized protein YBR292C; Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YBR292C is not an essential gene |
| VBA2 |
YBR293W |
Vacuolar basic amino acid transporter 2; Permease of basic amino acids in the vacuolar membrane |
| SUL1 |
YBR294W |
Solute carrier family 26 (sodium-independent sulfate anion transporter), member 11; High affinity sulfate permease of the SulP anion transporter family; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates |
| PCA1 |
YBR295W |
Cadmium transporting P-type ATPase; may also have a role in copper and iron homeostasis; stabilized by Cd binding, which prevents ubiquitination; S288C and other lab strains contain a G970R mutation which eliminates Cd transport function; Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily |
| PHO89 |
YBR296C |
Solute carrier family 20 (sodium-dependent phosphate transporter); Phosphate permease PHO89; Plasma membrane Na+/Pi cotransporter; active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification |
| TYC1 |
YBR296C-A |
Uncharacterized protein YBR296C-A; Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching |
| MAL33 |
YBR297W |
Maltose fermentation regulatory protein MAL33; MAL-activator protein; part of complex locus MAL3; nonfunctional in genomic reference strain S288C |
| GEX1 |
YCL073C |
Glutathione exchanger 1; Proton:glutathione antiporter; localized to the vacuolar and plasma membranes; imports glutathione from the vacuole and exports it through the plasma membrane; has a role in resistance to oxidative stress and modulation of the PKA pathway; GEX1 has a paralog, GEX2, that arose from a segmental duplication |
| VBA3 |
YCL069W |
Permease of basic amino acids in the vacuolar membrane; VBA3 has a paralog, VBA5, that arose from a segmental duplication |
| CHA1 |
YCL064C |
Catabolic L-serine/threonine dehydratase; Catabolic L-serine (L-threonine) deaminase; catalyzes the degradation of both L-serine and L-threonine; required to use serine or threonine as the sole nitrogen source, transcriptiony induced by serine and threonine; Belongs to the serine/threonine dehydratase family |
| VAC17 |
YCL063W |
Vacuole-related protein 17; Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p; involved in regulation of asymmetric inheritance of aggregated/misfolded proteins and age reset |
| MRC1 |
YCL061C |
S-phase checkpoint protein required for DNA replication; couples DNA helicase and polymerase; interacts with and stabilizes Pol2p at sted replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; defines a novel S-phase checkpoint with Hog1p that coordinates DNA replication and transcription upon osmostress; protects uncapped telomeres; Dia2p-dependent degradation mediates checkpoint recovery; mammalian claspin homolog |
| KRR1 |
YCL059C |
KRR1 sm subunit processome component; Nucleolar protein required for rRNA synthesis and ribosomal assembly; required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit; essential gene; Belongs to the KRR1 family |
| FYV5 |
YCL058C |
Protein involved in regulation of the mating pathway; binds with Matalpha2p to promoters of haploid-specific genes; required for survival upon exposure to K1 killer toxin; involved in ion homeostasis |
| ADF1 |
YCL058W-A |
Antisense of depressing factor protein 1; Transcriptional repressor encoded by the FYV5 antisense strand; negatively regulates transcription of FYV5 by binding to the promoter on the sense strand |
| MIC10 |
YCL057C-A |
Conserved component of the MICOS complex; MICOS (formerly MINOS or MitOS) is a mitochondrial inner membrane complex that extends into the intermembrane space and has a role in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane; forms a subcomplex with Mic12p and Mic27p whose assembly and stability requires cardiolipin; homo-oligomers cause membrane bending; ortholog of human MINOS1 |
| PRD1 |
YCL057W |
Saccharolysin; Zinc metoendopeptidase; found in the cytoplasm and intermembrane space of mitochondria; with Cym1p, involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins; protein abundance increases in response to DNA replication stress |
| PEX34 |
YCL056C |
Protein that regulates peroxisome populations; peroxisomal integral membrane protein; interacts with Pex11p, Pex25p, and Pex27p to control both constitutive peroxisome division and peroxisome morphology and abundance during peroxisome proliferation |
| KAR4 |
YCL055W |
Mrna m6a methyltransferase non-catalytic subunit; Karyogamy protein KAR4; Transcription factor required for response to pheromones; also required during meiosis; exists in two forms, a slower-migrating form more abundant during vegetative growth and a faster-migrating form induced by pheromone |
| RDT1 |
YCL054W-A |
Unknown |
| SPB1 |
YCL054W |
27S pre-rRNA (guanosine(2922)-2'-O)-methyltransferase; AdoMet-dependent methyltransferase; involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants |
| PBN1 |
YCL052C |
Protein PBN1; Component of glycosylphosphatidylinositol-mannosyltransferase I; essential component; required for the autocatalytic post-translational processing of the protease B precursor Prb1p; localizes to ER in lumenal orientation; homolog of mammalian PIG-X |
| LRE1 |
YCL051W |
Laminarase-resistance protein LRE1; Protein involved in control of cell w structure and stress response; direct inhibitor of the nuclear Dbf2 related (NDR) kinase Cbk1p-Mob2p; overproduction confers resistance to cell-w degrading enzymes; exhibits genetic interactions with genes involved in the cell w integrity pathway; LRE1 has a paralog, HLR1, that arose from the whole genome duplication |
| APA1 |
YCL050C |
Protein APA1; AP4A phosphorylase; bifunctional diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase and ADP sulfurylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; catalyzes phosphorolysis of dinucleoside oligophosphates, cleaving substrates' alpha/beta-anhydride bond and introducing Pi into the beta-position of the corresponding NDP formed; protein abundance increases under DNA replication stress; APA1 has a paralog, APA2, that arose from the whole genome duplication; Belongs to the ATP adenylyltransferase family |
| YCL049C |
YCL049C |
Uncharacterized protein YCL049C; Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene |
| YCL048W-A |
YCL048W-A |
Uncharacterized protein YCL048W-A; Putative protein of unknown function; SWAT-GFP and mCherry fusion proteins localize to the cell periphery and vacuole; YCL048W-A has a paralog, YDR524C-B, that arose from the whole genome duplication |
| SPS22 |
YCL048W |
Protein of unknown function; SPS22 has a paralog, SPS2, that arose from the whole genome duplication; redundant with Sps2p for the organization of the beta-glucan layer of the spore w |
| POF1 |
YCL047C |
Nicotinamide mononucleotide-specific adenylyltransferase (NMNAT); catalyzes the conversion of nicotinamide mononucleotide (NMN) to nicotinamide adenine dinucleotide (NAD+); role in the nicotinamide riboside (NR) salvage pathway of NAD+ biosynthesis; involved in NR and NAD+ homeostasis; ATPase involved in protein quality control and filamentation pathways; interacts physicy with Kss1p and suppresses the filamentation defect of a kss1 deletion |
| EMC1 |
YCL045C |
Member of conserved endoplasmic reticulum membrane complex; involved in efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; interacts with Gal80p; homologous to worm H17B01.4/EMC-1, fly CG2943, and human KIAA0090; Belongs to the EMC1 family |
| MGR1 |
YCL044C |
Subunit of the mitochondrial (mt) i-AAA protease supercomplex; i-AAA degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr3p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA |
| PDI1 |
YCL043C |
Protein disulfide isomerase; multifunctional oxidoreductase of the ER lumen, essential for disulfide bond formation in secretory and cell-surface proteins, processing of non-native disulfide bonds; Ero1p activator; complexes with exomannosidase, Mnl1p to facilitate the recognition of misfolded glycoproteins and the trimming of glycan Man8GlcNAc2 to Man7GlcNAc2 on substrates, thereby accelerating ERAD; PDI1 has a paralog, EUG1, that arose from the whole genome duplication |
| YCL042W |
YCL042W |
Putative uncharacterized protein ycl042w; Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm |
| GLK1 |
YCL040W |
Glucokinase-1; Glucokinase; catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources; GLK1 has a paralog, EMI2, that arose from the whole genome duplication; Belongs to the hexokinase family |
| GID7 |
YCL039W |
Glucose-induced degradation protein 7; Subunit of GID Complex that binds directly to central component Vid30p; GID complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; Gid7p contains six WD40 repeats; computational analysis suggests that Gid7p and Moh1p have similar functions |
| ATG22 |
YCL038C |
Autophagy-related protein 22; Vacuolar integral membrane protein required for efflux of amino acids; required for efflux of amino acids during autophagic body breakdown in the vacuole; null mutation causes a gradual loss of viability during starvation |
| SRO9 |
YCL037C |
Cytoplasmic RNA-binding protein; shuttles between nucleus and cytoplasm and is exported from the nucleus in an mRNA export-dependent manner; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication |
| GFD2 |
YCL036W |
Good for full DBP5 activity protein 2; Protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; GFD2 has a paralog, YDR514C, that arose from the whole genome duplication |
| GRX1 |
YCL035C |
Glutaredoxin-1; Glutathione-dependent disulfide oxidoreductase; hydroperoxide and superoxide-radical responsive, heat-stable, with active site cysteine pair; protects cells from oxidative damage; GRX1 has a paralog, GRX2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress |
| LSB5 |
YCL034W |
Protein involved in membrane-trafficking events at plasma membrane; interacts with actin regulators Sla1p and Las17p, ubiquitin, Arf3p to couple actin dynamics to membrane trafficking processes; similar structure to GGA family of proteins with N-terminal VHS domain and GAT domain; binds Las17p, which is homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly, actin polymerization; may mediate disassembly of Pan1 complex from endocytic coat; Belongs to the LSB5 family |
| MXR2 |
YCL033C |
Methionine-R-sulfoxide reductase; involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR1; involved in the regulation of lifespan |
| STE50 |
YCL032W |
Adaptor protein for various signaling pathways; involved in mating response, invasive/filamentous growth, osmotolerance; acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction |
| RRP7 |
YCL031C |
Ribosomal RNA-processing protein 7; Essential protein involved in rRNA processing and ribosome biogenesis; protein abundance increases in response to DNA replication stress |
| HIS4 |
YCL030C |
Histidine biosynthesis trifunctional protein; Multifunctional enzyme containing phosphoribosyl-ATP pyrophosphatase; phosphoribosyl-AMP cyclohydrolase, and histidinol dehydrogenase activities; catalyzes the second, third, ninth and tenth steps in histidine biosynthesis |
| BIK1 |
YCL029C |
Nuclear fusion protein BIK1; Microtubule-associated protein; component of the interface between microtubules and kinetochore, involved in sister chromatid separation; essential in polyploid cells but not in haploid or diploid cells; ortholog of mammalian CLIP-170 |
| RNQ1 |
YCL028W |
Prion domain-containing protein rnq1; [PIN(+)] prion; an infectious protein conformation that is genery an ordered protein aggregate |
| FUS1 |
YCL027W |
Nuclear fusion protein FUS1; Membrane protein localized to the shmoo tip; required for cell fusion; expression regulated by mating pheromone; proposed to coordinate signaling, fusion, and polarization events required for fusion; potential Cdc28p substrate |
| HBN1 |
YCL026C-B |
Putative nitroreductase HBN1; Protein of unknown function; similar to bacterial nitroreductases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein becomes insoluble upon intracellular iron depletion; protein abundance increases in response to DNA replication stress |
| FRM2 |
YCL026C-A |
Fatty acid repression mutant protein 2; Type II nitroreductase, using NADH as reductant; mutants are defective in fatty acid mediated repression of genes involved in fatty acid biosynthesis indicative of a role in lipid signaling; involved in the oxidative stress response; transcription induction by cadmium and selenite indicates a possible role in the metal stress response; expression induced in cells treated with the mycotoxin patulin |
| AGP1 |
YCL025C |
Low-affinity amino acid permease with broad substrate range; involved in uptake of asparagine, glutamine, and other amino acids; expression regulated by SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); AGP1 has a paralog, GNP1, that arose from the whole genome duplication; Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family |
| KCC4 |
YCL024W |
Probable serine/threonine-protein kinase KCC4; Protein kinase of the bud neck involved in the septin checkpoint; associates with septin proteins, negatively regulates Swe1p by phosphorylation, shows structural homology to bud neck kinases Gin4p and Hsl1p; KCC4 has a paralog, GIN4, that arose from the whole genome duplication; Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NIM1 subfamily |
| YNCC0001C |
YNCC0001C |
Unknown |
| YCL021W-A |
YCL021W-A |
Putative uncharacterized protein ycl021w-a; Putative protein of unknown function; SWAT-GFP and mCherry fusion proteins localize to the vacuole |
| SUP53 |
YNCC0002W |
Unknown |
| LEU2 |
YCL018W |
Beta-isopropylmalate dehydrogenase (IMDH); catalyzes the third step in the leucine biosynthesis pathway; can additiony catalyze the conversion of beta-ethylmalate into alpha-ketovalerate; Belongs to the isocitrate and isopropylmalate dehydrogenases family |
| NFS1 |
YCL017C |
Cysteine desulfurase, mitochondrial; Cysteine desulfurase; involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria |
| DCC1 |
YCL016C |
Sister chromatid cohesion protein DCC1; Subunit of a complex with Ctf8p and Ctf18p; shares some components with Replication Factor C; required for sister chromatid cohesion and telomere length maintenance; Belongs to the DCC1 family |
| BUD3 |
YCL014W |
Bud site selection protein 3; Guanine nucleotide exchange factor (GEF) for Cdc42p; activates Cdc42p in early G1, accounting for the first stage of biphasic activation, with Cdc24p accounting for the second stage in late G1; involved in the Cdc42p-mediated assembly of the axial landmark that dictates the site for the next round of budding, resulting in the axial budding pattern observed in haploids; localizes with septins to the bud neck contractile ring in mitosis |
| YCL012C |
YCL012C |
UPF0357 protein YCL012C; Protein of unknown function; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum and vacuole respectively; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene; Belongs to the UPF0357 family |
| GBP2 |
YCL011C |
Single-strand telomeric DNA-binding protein GBP2; Poly(A+) RNA-binding protein; key surveillance factor for the selective export of spliced mRNAs from the nucleus to the cytoplasm; preference for intron-containing genes; similar to Npl3p; also binds single-stranded telomeric repeat sequence in vitro; relocalizes to the cytosol in response to hypoxia; GBP2 has a paralog, HRB1, that arose from the whole genome duplication |
| SGF29 |
YCL010C |
SAGA-associated factor 29; Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes; HAT/Core module also contains Gcn5p, Ngg1p, and Ada2p; binds methylated histone H3K4; involved in transcriptional regulation through SAGA and TBP recruitment to target promoters and H3 acetylation; Belongs to the SGF29 family |
| ILV6 |
YCL009C |
Acetolactate synthase sm subunit, mitochondrial; Regulatory subunit of acetolactate synthase; acetolactate synthase catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria |
| STP22 |
YCL008C |
Suppressor protein STP22 of temperature-sensitive alpha-factor receptor and arginine permease; Component of the ESCRT-I complex; ESCRT-I is involved in ubiquitin-dependent sorting of proteins into the endosome; prevents polyubiquitination of the arrestin-related protein Rim8p, thereby directing its monoubiquitination by Rsp5p; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype;; Belongs to the ubiquitin-conjugating enzyme family. UEV subfamily |
| VMA9 |
YCL005W-A |
Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis |
| SNR43 |
YNCC0003C |
Unknown |
| LDB16 |
YCL005W |
Protein involved in lipid droplet (LD) assembly; forms a complex with Sei1p at ER-LD contact sites, stabilizing contact sites; ensures that LDs bud from the ER towards the cytosolic side of the membrane; null mutants have decreased net negative cell surface charge and localized accumulation of phosphatidic acid (PA) marker proteins; GFP-fusion protein expression is induced in response to MMS; null mutant can be complemented by the human seipin, BSCL2 |
| PGS1 |
YCL004W |
CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase; Phosphatidylglycerolphosphate synthase; catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis |
| YCL002C |
YCL002C |
Putative uncharacterized protein ycl002c; Putative protein of unknown function; YCL002C is not an essential gene |
| RER1 |
YCL001W |
Protein involved in retention of membrane proteins; including Sec12p, in the ER; localized to Golgi; functions as a retrieval receptor in returning membrane proteins to the ER |
| YCL001W-A |
YCL001W-A |
Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III; Belongs to the eukaryotic release factor 1 family. Pelota subfamily. Highly divergent |
| YCL001W-B |
YCL001W-B |
Putative protein of unknown function; present in a region duplicated between chromosomes XIV and III; YCL001W-B has a paralog, DOM34, that arose from the whole genome duplication; Belongs to the eukaryotic release factor 1 family. Pelota subfamily. Highly divergent |
| YCR001W |
YCR001W |
Uncharacterized protein YCR001W; Putative protein of unknown function; conserved among S. cerevisiae strains; YCR001W is not an essential gene |
| CDC10 |
YCR002C |
Cell division control protein 10; Component of the septin ring, required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells; N-terminus interacts with phosphatidylinositol-4,5-bisphosphate; protein abundance increases under DNA damage stress |
| MRPL32 |
YCR003W |
Mitochondrial 54s ribosomal protein yml32; Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress |
| YCP4 |
YCR004C |
Flavodoxin-like fold family protein; Flavoprotein-like protein YCP4; Protein of unknown function; has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies |
| CIT2 |
YCR005C |
Citrate synthase, peroxisomal isozyme involved in glyoxylate cycle; catalyzes condensation of acetyl coenzyme A and oxaloacetate to form citrate; expression is controlled by Rtg1p and Rtg2p transcription factors; SCF-Ucc1 regulates level of Cit2p to maintain citrate homeostasis; oxaloacetate-dependent positive feedback loop inhibits Cit2p ubiquitination; CIT2 has a paralog, CIT1, that arose from the whole genome duplication |
| YCR006C |
YCR006C |
Uncharacterized protein YCR006C; Putative protein of unknown function; conserved among S. cerevisiae strains; YCR006C is not an essential gene |
| SUF2 |
YNCC0004C |
Unknown |
| YCR007C |
YCR007C |
DUP240 protein YCR007C; Putative integral membrane protein; member of DUP240 gene family; SWAT-GFP and mCherry fusion proteins localize to the cell periphery and vacuole; YCR007C is not an essential gene |
| YNCC0005W |
YNCC0005W |
Unknown |
| SAT4 |
YCR008W |
Serine/threonine-protein kinase HAL4/SAT4; Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; overexpression affects the Fe-S and lipoamide containing proteins in the mitochondrion; required for lipoylation of Lat1p, Kgd2p and Gcv3p; partiy redundant with Hal5p; has similarity to Npr1p; localizes to the cytoplasm and mitochondrion |
| RVS161 |
YCR009C |
Reduced viability upon starvation protein 161; Amphiphysin-like lipid raft protein; N-BAR domain protein that interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress |
| ADY2 |
YCR010C |
Accumulation of dyads protein 2; Acetate transporter required for normal sporulation; phosphorylated in mitochondria; ADY2 has a paralog, ATO2, that arose from the whole genome duplication |
| ADP1 |
YCR011C |
Putative atp-dependent permease adp1; Putative ATP-dependent permease of the ABC transporter family; Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily |
| PGK1 |
YCR012W |
3-phosphoglycerate kinase; catalyzes transfer of high-energy phosphoryl groups from the acyl phosphate of 1,3-bisphosphoglycerate to ADP to produce ATP; key enzyme in glycolysis and gluconeogenesis |
| POL4 |
YCR014C |
DNA polymerase IV; undergoes pair-wise interactions with Dnl4p-Lif1p and Rad27p to mediate repair of DNA double-strand breaks by non-homologous end joining (NHEJ); homologous to mammalian DNA polymerase beta |
| CTO1 |
YCR015C |
UPF0655 protein YCR015C; Protein required for cold tolerance; involved in phosphate uptake; YCR015C is not an essential gene; Belongs to the UPF0655 family |
| SNR33 |
YNCC0006C |
Unknown |
| YCR016W |
YCR016W |
Uncharacterized protein YCR016W; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis |
| CWH43 |
YCR017C |
Protein CWH43; GPI lipid remodelase; responsible for introducing ceramides into GPI anchors having a C26:0 fatty acid in sn-2 of the glycerol moiety; can also use lyso-GPI protein anchors and various base resistant lipids as substrates; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is syntheticy lethal with pkc1 deletion; In the C-terminal section; belongs to the PGAP2IP family |
| SRD1 |
YCR018C |
Pre-rRNA-processing protein SRD1; Protein involved in the processing of pre-rRNA to mature rRNA; contains a C2/C2 zinc finger motif; srd1 mutation suppresses defects caused by the rrp1-1 mutation |
| YNCC0008W |
YNCC0008W |
Unknown |
| YNCC0009C |
YNCC0009C |
Unknown |
| MAK32 |
YCR019W |
Protein mak32; Protein necessary for stability of L-A dsRNA-containing particles |
| PET18 |
YCR020C |
Protein of unknown function; has weak similarity to proteins involved in thiamin metabolism; expression is induced in the absence of thiamin |
| MAK31 |
YCR020C-A |
N-alpha-acetyltransferase 38, NatC auxiliary subunit; Non-catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus; member of the Sm protein family |
| HTL1 |
YCR020W-B |
High temperature lethal protein 1; Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance |
| HSP30 |
YCR021C |
30 kDa heat shock protein; Negative regulator of the H(+)-ATPase Pma1p; stress-responsive protein; hydrophobic plasma membrane localized; induced by heat shock, ethanol treatment, weak organic acid, glucose limitation, and entry into stationary phase; Belongs to the archaeal/bacterial/fungal opsin family |
| YCR022C |
YCR022C |
Uncharacterized protein YCR022C; Putative protein of unknown function; conserved among S. cerevisiae strains; YCR022C is not an essential gene |
| YCR023C |
YCR023C |
Vacuolar membrane protein of unknown function; member of the multidrug resistance family; YCR023C is not an essential gene |
| SLM5 |
YCR024C |
Asparagine--tRNA ligase, mitochondrial; Mitochondrial asparaginyl-tRNA synthetase |
| YCR024C-B |
YCR024C-B |
Uncharacterized protein YCR024C-B; Putative protein of unknown function; identified by expression profiling and mass spectrometry |
| PMP1 |
YCR024C-A |
Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; sm single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specificy segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication |
| YCR025C |
YCR025C |
Uncharacterized protein YCR025C; Putative protein of unknown function; conserved across S. cerevisiae strains; YCR025C is not an essential gene |
| NPP1 |
YCR026C |
Ectonucleotide pyrophosphatase/phosphodiesterase 1; Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; involved in spore w assembly; NPP1 has a paralog, NPP2, that arose from the whole genome duplication, and an npp1 npp2 double mutant exhibits reduced dityrosine fluorescence relative to the single mutants |
| RHB1 |
YCR027C |
Rheb-like protein RHB1; Putative Rheb-related GTPase; involved in regulating canavanine resistance and arginine uptake; member of the Ras superfamily of G-proteins |
| YNCC0010C |
YNCC0010C |
Unknown |
| FEN2 |
YCR028C |
Pantothenate transporter FEN2; Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph; relocalizes from vacuole to cytoplasm upon DNA replication stress |
| RIM1 |
YCR028C-A |
ssDNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication; stimulates utilization by Mip1p DNA polymerase of RNA primers synthesized by Rpo41p |
| SYP1 |
YCR030C |
Suppressor of yeast profilin deletion; Negative regulator of WASP-Arp23 complex; involved in endocytic site formation; directly inhibits Las17p stimulation of Arp23 complex-mediated actin assembly in vitro; may regulate assembly and disassembly of the septin ring; colocalizes and interacts with septin subunits; potential role in actin cytoskeletal organization |
| SNR65 |
YNCC0011W |
Unknown |
| RPS14A |
YCR031C |
Protein component of the sm (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication |
| SNR189 |
YNCC0012C |
Unknown |
| BPH1 |
YCR032W |
Wd repeat and fyve domain-containing protein 3; Protein homologous to Chediak-Higashi syndrome and Beige proteins; both of which are implicated in disease syndromes in human and mouse, respectively, due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting |
| SNT1 |
YCR033W |
Probable DNA-binding protein SNT1; Subunit of the Set3C deacetylase complex; interacts directly with the Set3C subunit, Sif2p; putative DNA-binding protein; mutant has increased aneuploidy tolerance; relocalizes to the cytosol in response to hypoxia |
| ELO2 |
YCR034W |
Elongation of fatty acids protein 2; Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; ELO2 has a paralog, ELO1, that arose from the whole genome duplication; lethality of the elo2 elo3 double null mutation is functiony complemented by human ELOVL1 and weakly complemented by human ELOVL3 or ELOV7 |
| RRP43 |
YCR035C |
Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress |
| RBK1 |
YCR036W |
Putative ribokinase; Belongs to the carbohydrate kinase PfkB family. Ribokinase subfamily |
| PHO87 |
YCR037C |
Low-affinity inorganic phosphate (Pi) transporter; acts upstream of Pho81p in regulation of the PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication |
| YCL068C |
YCL068C |
Uncharacterized protein; Putative protein of unknown function |
| HMRA2 |
YCR096C |
Silenced copy of a2 at HMR; similarity to Alpha2p; required along with a1p for inhibiting expression of the HO endonuclease in a/alpha HO/HO diploid cells with an active mating-type interconversion system; Belongs to the TALE/M-ATYP homeobox family |
| MATALPHA1 |
YCR040W |
Silenced mating-type protein ALPHA1; Transcriptional co-activator that regulates mating-type-specific genes; targets the transcription factor Mcm1p to the promoters of alpha-specific genes; one of two genes encoded by the MATalpha mating type cassette |
| YCR041W |
YCR041W |
Uncharacterized protein YCR041W; Protein of unknown function; overexpression suppresses the high-frequency loss of mini-chromosomes, probably by increasing the rate of proper chromosome segregation; translated gene product of YCR041W, but not its transcript, is responsible for suppression; suppression ability of YCR041W is completely dependent on silencing protein Sir4p |
| TAF2 |
YCR042C |
Transcription initiation factor tfiid subunit 2; TFIID subunit (150 kDa); involved in RNA polymerase II transcription initiation |
| YCR043C |
YCR043C |
Uncharacterized protein YCR043C; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi apparatus; YCR043C is not an essential gene |
| PER1 |
YCR044C |
Post-gpi attachment to proteins factor 3; Protein of the endoplasmic reticulum; required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functiony complemented by human ortholog PERLD1 |
| RRT12 |
YCR045C |
Subtilase-type proteinase RRT12; Probable subtilisin-family protease; role in formation of the dityrosine layer of spore ws; localizes to the spore w and also the nuclear envelope and ER region in mature spores |
| IMG1 |
YCR046C |
54S ribosomal protein IMG1, mitochondrial; Mitochondrial ribosomal protein of the large subunit; required for respiration and for maintenance of the mitochondrial genome |
| BUD23 |
YCR047C |
Methyltransferase that methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; diploid mutant displays random budding pattern; functional homolog of human WBSCR22; Belongs to the class I-like SAM-binding methyltransferase superfamily. BUD23/WBSCR22 family |
| ARE1 |
YCR048W |
Sterol O-acyltransferase 1; Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the absence of oxygen; ARE1 has a paralog, ARE2, that arose from the whole genome duplication; Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily |
| YCR050C |
YCR050C |
Uncharacterized protein YCR050C; Non-essential protein of unknown function; deletion mutant is syntheticy sick or lethal with alpha-synuclein |
| YCR051W |
YCR051W |
Ankyrin repeat-containing protein YCR051W; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene |
| RSC6 |
YCR052W |
Component of the RSC chromatin remodeling complex; essential for mitotic growth; RSC6 has a paralog, SNF12, that arose from the whole genome duplication |
| THR4 |
YCR053W |
Threonine synthase; conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway |
| CTR86 |
YCR054C |
Copper transport protein 86; Essential protein of unknown function; with orthologs in Ashbya gossypii and Candida albicans; similar to human ATXN10, mutations in which cause spinocerebellar ataxia type 10; codon usage corresponds to that observed for yeast genes expressed at low levels; relative distribution to the nucleus increases upon DNA replication stress |
| PWP2 |
YCR057C |
Periodic tryptophan protein 2; Conserved 90S pre-ribosomal component; essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis |
| YIH1 |
YCR059C |
Protein IMPACT homolog; Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT; Belongs to the IMPACT family |
| TAH1 |
YCR060W |
TPR repeat-containing protein associated with Hsp90; Component of conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly of large protein complexes such as box C/D snoRNPs and RNA polymerase II; contains a single TPR domain with at least two TPR motifs; plays a role in determining prion variants |
| TVS1 |
YCR061W |
Uncharacterized membrane protein YCR061W; Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation |
| SUP61 |
YNCC0013W |
Unknown |
| BUD31 |
YCR063W |
Pre-mRNA-splicing factor BUD31; Component of the SF3b subcomplex of the U2 snRNP; increases efficiency of first and second step pre-mRNA splicing; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis |
| HCM1 |
YCR065W |
Forkhead transcription factor; drives S-phase activation of genes involved in chromosome segregation, spindle dynamics, budding; also activates genes involved in respiration, use of alternative energy sources (like proline), NAD synthesis, oxidative stress resistance; key factor in early adaptation to nutrient deficiency and diauxic shift; suppressor of calmodulin mutants with specific SPB assembly defects; ortholog of C. elegans lifespan regulator PHA-4 |
| RAD18 |
YCR066W |
Postreplication repair E3 ubiquitin-protein ligase RAD18; E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA |
| SED4 |
YCR067C |
Putative guanine nucleotide-exchange factor SED4; Integral ER membrane protein that stimulates Sar1p GTPase activity; involved in COPII vesicle budding through disassociation of coat proteins from membranes onto liposomes; binds Sec16p; SED4 has a paralog, SEC12, that arose from the whole genome duplication |
| ATG15 |
YCR068W |
Putative lipase ATG15; Phospholipase; preferentiy hydrolyses phosphatidylserine, with minor activity against cardiolipin and phosphatidylethanolamine; required for lysis of autophagic and CVT bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway; required for the maintenance of lipid droplet quantity after the diauxic shift; regulates lipolysis; expression regulated by Yap1p during autophagy; Belongs to the AB hydrolase superfamily. Lipase family |
| CPR4 |
YCR069W |
Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway; CPR4 has a paralog, CPR8, that arose from the whole genome duplication |
| IMG2 |
YCR071C |
54S ribosomal protein IMG2, mitochondrial; Mitochondrial ribosomal protein of the large subunit; conserved in metazoa, with similarity to human mitochondrial ribosomal protein MRPL49 |
| RSA4 |
YCR072C |
WD-repeat protein involved in ribosome biogenesis; may interact with ribosomes; required for maturation and efficient intra-nuclear transport or pre-60S ribosomal subunits, localizes to the nucleolus; Belongs to the NLE1/RSA4 family |
| SSK22 |
YCR073C |
Serine/threonine-protein kinase SSK22; MAP kinase kinase kinase of HOG1 mitogen-activated signaling pathway; functiony redundant with Ssk2p; interacts with and is activated by Ssk1p; phosphorylates Pbs2p; SSK22 has a paralog, SSK2, that arose from the whole genome duplication |
| SOL2 |
YCR073W-A |
6-phosphogluconolactonase-like protein 2; Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication |
| ERS1 |
YCR075C |
Protein involved in cystine transport; localizes to the vacuole, plasma membrane and endosome; similarity to human cystinosin, a H(+)-driven transporter involved in L-cystine export from lysosomes and implicated in the disease cystinosis; contains seven transmembrane domains; mutation is functiony complemented by human CTNS |
| EGO2 |
YCR075W-A |
Uncharacterized protein YCR075W-A; Component of the EGO and GSE complexes; identified by homology to Ashbya gossypii; YCR075W-A has a paralog, YNR034W-A, that arose from the whole genome duplication |
| FUB1 |
YCR076C |
Silencing boundary-establishment protein FUB1; Proteasome-binding protein; interacts physicy with multiple subunits of the 20S proteasome and geneticy with genes encoding 20S core particle and 19S regulatory particle subunits; exhibits boundary activity which blocks the propagation of heterochromatic silencing; contains a PI31 proteasome regulator domain and sequence similarity with human PSMF1, a proteasome inhibitor; not an essential gene |
| PAT1 |
YCR077C |
DNA topoisomerase 2-associated protein PAT1; Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; binds to mRNAs under glucose starvation, most often in the 3' UTR; functiony linked to Pab1p; forms cytoplasmic foci upon DNA replication stress; phosphorylation by PKA inhibits P body foci formation; Belongs to the PAT1 family |
| PTC6 |
YCR079W |
[Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 2, mitochondrial; Mitochondrial type 2C protein phosphatase (PP2C); has similarity to mammalian PP1Ks; involved in mitophagy; null mutant is sensitive to rapamycin and has decreased phosphorylation of the Pda1 subunit of pyruvate dehydrogenase |
| SRB8 |
YCR081W |
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression |
| AHC2 |
YCR082W |
Component of the ADA histone acetyltransferase complex; Ahc2p and Ahc1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ahc1p to the complex |
| TRX3 |
YCR083W |
Thioredoxin-3, mitochondrial; Mitochondrial thioredoxin; highly conserved oxidoreductase required to maintain the redox homeostasis of the cell, forms the mitochondrial thioredoxin system with Trr2p, redox state is maintained by both Trr2p and Glr1p |
| TUP1 |
YCR084C |
General transcriptional corepressor TUP1; General repressor of transcription; forms complex with Cyc8p, involved in the establishment of repressive chromatin structure through interactions with histones H3 and H4, appears to enhance expression of some genes |
| YCR085W |
YCR085W |
Uncharacterized protein YCR085W; Putative protein of unknown function; conserved among S. cerevisiae strains; YCR085W is not an essential gene |
| CSM1 |
YCR086W |
Monopolin complex subunit CSM1; Nucleolar protein that mediates homolog segregation during meiosis I; forms a complex with Lrs4p and then Mam1p at kinetochores; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation |
| YCR087C-A |
YCR087C-A |
Cell growth-regulating nucleolar protein; UPF0743 protein YCR087C-A; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene; Belongs to the UPF0743 family |
| ABP1 |
YCR088W |
Actin-binding protein of the cortical actin cytoskeleton; important for activation of the Arp2/3 complex that plays a key role actin in cytoskeleton organization; inhibits barbed-end actin filament elongation; phosphorylation within its Proline-Rich Regio, mediated by Cdc28p and Pho85p, protects Abp1p from proteolysis mediated by its own PEST sequences; mammalian homolog of HIP-55 (hematopoietic progenitor kinase 1 [HPK1]-interacting protein of 55 kDa) |
| FIG2 |
YCR089W |
Factor-induced gene 2 protein; Cell w adhesin, expressed specificy during mating; may be involved in maintenance of cell w integrity during mating; FIG2 has a paralog, AGA1, that arose from the whole genome duplication |
| YCR090C |
YCR090C |
UPF0587 protein YCR090C; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YCR090C is not an essential gene |
| KIN82 |
YCR091W |
Serine/threonine-protein kinase KIN82; Putative serine/threonine protein kinase; implicated in the regulation of phospholipid asymmetry through the activation of phospholipid translocases (flippases); involved in the phosphorylation of upstream inhibitory kinase Ypk1p along with Fpk1p; has a redundant role in the cellular response to mating pheromone; KIN82 has a paralog, FPK1, that arose from the whole genome duplication |
| MSH3 |
YCR092C |
Mismatch repair protein; forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability; Belongs to the DNA mismatch repair MutS family. MSH3 subfamily |
| CDC39 |
YCR093W |
General negative regulator of transcription subunit 1; Subunit of the CCR4-NOT1 core complex; this complex has multiple roles in the regulation of mRNA levels including regulation of transcription and destabilization of mRNA by deadenylation; basal transcription factor that increases initiation and elongation; activates the ATPase activity of Dhh1p, resulting in processing body disassembly |
| CDC50 |
YCR094W |
Cell division control protein 50; Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication |
| OCA4 |
YCR095C |
Protein oca4; Cytoplasmic protein required for replication of Brome mosaic virus; S. cerevisiae is a model system for studying replication of positive-strand RNA viruses in their natural hosts |
| YCR095W-A |
YCR095W-A |
Putative uncharacterized protein ycr095w-a; Putative protein of unknown function |
| HMLALPHA2 |
YCL067C |
Silenced copy of ALPHA2 at HML; homeobox-domain protein that associates with Mcm1p in haploid cells to repress a-specific gene expression and interacts with a1p in diploid cells to repress haploid-specific gene expression; Belongs to the TALE/M-ATYP homeobox family |
| HMRA1 |
YCR097W |
Silenced mating-type protein a1; Silenced copy of a1 at HMR; homeobox corepressor that interacts with Alpha2p to repress haploid-specific gene transcription in diploid cells |
| YNCC0014W |
YNCC0014W |
Unknown |
| GIT1 |
YCR098C |
Glycerophosphoinositol transporter 1; Plasma membrane permease; mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability; Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family |
| YCR099C |
YCR099C |
Uncharacterized protein YCR099C; Putative protein of unknown function |
| EMA35 |
YCR100C |
Uncharacterized protein YCR100C; Putative protein of unknown function |
| YCR101C |
YCR101C |
Uncharacterized protein YCR101C; Putative protein of unknown function; localizes to the membrane fraction; YCR101C is not an essential gene |
| YLR460C |
YLR460C |
Uncharacterized protein YLR460C; Member of the quinone oxidoreductase family; up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine |
| AAD4 |
YDL243C |
Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin; members of the AAD gene family comprise three pairs (AAD3 + AAD15, AAD6/AAD16 + AAD4, AAD10 + AAD14) whose two genes are more related to one another than to other members of the family |
| YDL242W |
YDL242W |
Uncharacterized protein YDL242W; Putative protein of unknown function; conserved across S. cerevisiae strains |
| YDL241W |
YDL241W |
Uncharacterized protein YDL241W; Putative protein of unknown function; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum; YDL241W is not an essential gene |
| LRG1 |
YDL240W |
Rho-GTPase-activating protein LRG1; GTPase-activating protein (GAP); contains Rho1p-specific GAP activity, interacting with activated forms of Rho1p; functions along with Sac7p as a negative regulator of the Pkc1p-mediated cell w integrity signaling pathway; negative regulator of cell w 1,3-beta-glucan biosynthesis; required for efficient cell fusion; contains a RhoGAP domain and three Lin-11-Isl1-Mec-3 (LIM) domains |
| ADY3 |
YDL239C |
Accumulates dyads protein 3; Protein required for spore w formation; subunit of the leading edge protein (LEP) complex (Ssp1-Ady3-Don1-Irc10) that forms a ring-like structure at the leading edge of the prospore membrane during meiosis II; mediates assembly of the LEP complex, formation of the ring-like structure via interaction with spindle pole body components and prospore membrane maturation; potentiy phosphorylated by Cdc28p; ADY3 has a paralog, CNM67, that arose from the whole |
| GUD1 |
YDL238C |
Guanine deaminase; a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentiy-growing cultures but expression is increased in post-diauxic and stationary-phase cultures |
| AIM6 |
YDL237W |
Altered inheritance of mitochondria protein 6; Protein of unknown function; required for respiratory growth; YDL237W is not an essential gene |
| PHO13 |
YDL236W |
4-nitrophenylphosphatase; Conserved phosphatase acting as a metabolite repair enzyme; shows specific dephosphorylating activity on two side-products of central carbohydrate metabolism, 2-phosphoglycolate and 4-phosphoerythronate; alkaline phosphatase specific for p-nitrophenyl phosphate; also has protein phosphatase activity; human ortholog PGP shows similar substrate specificity, deletion causes similar metabolite accumulation phenotypes, suggesting conserved role in eliminating glycolytic byproducts |
| YPD1 |
YDL235C |
Osmotic stress-responsive phosphorelay intermediate sensor protein; phosphorylated by the plasma membrane sensor Sln1p in response to osmotic stress and then in turn phosphorylates the response regulators Ssk1p in the cytosol and Skn7p in the nucleus; Belongs to the YPD1 family |
| GYP7 |
YDL234C |
GTPase-activating protein for yeast Rab family members; members include Ypt7p (most effective), Ypt1p, Ypt31p, and Ypt32p (in vitro); involved in vesicle mediated protein trafficking; contains a PH-like domain |
| MFG1 |
YDL233W |
Regulator of filamentous growth; interacts with FLO11 promoter and regulates FLO11 expression; binds to transcription factors Flo8p and Mss11p; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YDL233W is not an essential gene; Belongs to the MFG1 family |
| OST4 |
YDL232W |
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST4; Subunit of the oligosaccharyltransferase complex of the ER lumen; complex catalyzes protein asparagine-linked glycosylation; type I membrane protein required for incorporation of Ost3p or Ost6p into the OST complex |
| BRE4 |
YDL231C |
Protein bre4; Zinc finger protein containing five transmembrane domains; null mutant exhibits strongly fragmented vacuoles and sensitivity to brefeldin A, a drug which is known to affect intracellular transport |
| PTP1 |
YDL230W |
Tyrosine-protein phosphatase 1; Phosphotyrosine-specific protein phosphatase; dephosphorylates a broad range of substrates in vivo, including Fpr3p; localized to the cytoplasm and the mitochondria; proposed to be a negative regulator of filamentation; Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily |
| SSB1 |
YDL229W |
Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; functions with J-protein partner Zuo1p; may be involved in folding of newly-made polypeptide chains; member of the HSP70 family; interacts with phosphatase subunit Reg1p; SSB1 has a paralog, SSB2, that arose from the whole genome duplication; Belongs to the heat shock protein 70 family. Ssb-type Hsp70 subfamily |
| HO |
YDL227C |
Homothic switching endonuclease; Site-specific endonuclease; required for gene conversion at the MAT locus (homothic switching) through the generation of a ds DNA break; expression restricted to mother cells in late G1 as controlled by Swi4p-Swi6p, Swi5p, and Ash1p |
| GCS1 |
YDL226C |
ADP-ribosylation factor GTPase activating protein (ARF GAP); involved in ER-Golgi transport; required for prospore membrane formation; regulates phospholipase Spo14p; shares functional similarity with Glo3p; GCS1 has a paralog, SPS18, that arose from the whole genome duplication |
| SHS1 |
YDL225W |
Seventh homolog of septin 1; Component of the septin ring that is required for cytokinesis; present at the ends of rod-like septin hetero-oligomers; C-terminal extension is important for recruitment of Bni5p to the mother-bud neck, which in turn is required for Myo1p recruitment and cytokinesis; undergoes sumoylation and phosphorylation during mitosis; protein abundance increases in response to DNA replication stress |
| WHI4 |
YDL224C |
Protein WHI4; Putative RNA binding protein; regulates the cell size requirement for passage through Start and commitment to cell division; WHI4 has a paralog, WHI3, that arose from the whole genome duplication |
| HBT1 |
YDL223C |
Shmoo tip protein, substrate of Hub1p ubiquitin-like protein; mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis; HBT1 has a paralog, YNL195C, that arose from the whole genome duplication |
| FMP45 |
YDL222C |
SUR7 family protein FMP45; Integral membrane protein localized to mitochondria; required for sporulation and maintaining sphingolipid content; similar to SUR7; FMP45 has a paralog, YNL194C, that arose from the whole genome duplication |
| CDC13 |
YDL220C |
Cell division control protein 13; Single stranded DNA-binding protein found at TG1-3 telomere G-tails; key roles in regulation of telomerase, telomere end protection, conventional telomere replication; regulates telomere replication through recruitment of specific sub-complexes, essential function is telomere capping; forms homodimer via N-terminus; disruption of dimerization leads to short telomeres; autophagy and proteasome are involved in Cdc13p degradation; differentiy phosphorylated through cell cycle |
| DTD1 |
YDL219W |
D-Tyr-tRNA(Tyr) deacylase; functions in protein translation, may affect nonsense suppression via alteration of the protein synthesis machinery; ubiquitous among eukaryotes; Belongs to the DTD family |
| YDL218W |
YDL218W |
Putative protein of unknown function; YDL218W transcription is regulated by Azf1p and induced by starvation and aerobic conditions; expression also induced in cells treated with the mycotoxin patulin |
| TIM22 |
YDL217C |
Mitochondrial import inner membrane translocase subunit TIM22; Essential core component of the mitochondrial TIM22 complex; involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported; Belongs to the Tim17/Tim22/Tim23 family |
| RRI1 |
YDL216C |
Catalytic subunit of the COP9 signalosome (CSN) complex; acts as an isopeptidase in cleaving the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; metoendopeptidase involved in the adaptation to pheromone signaling; involved in modulation of genes controlling amino acid and lipid metabolism, and ergosterol biosynthesis; Belongs to the peptidase M67A family. CSN5 subfamily |
| GDH2 |
YDL215C |
NAD(+)-dependent glutamate dehydrogenase; degrades glutamate to ammonia and alpha-ketoglutarate; expression sensitive to nitrogen catabolite repression and intracellular ammonia levels; geneticy interacts with GDH3 by suppressing stress-induced apoptosis |
| PRR2 |
YDL214C |
Serine/threonine-protein kinase PRR2; Serine/threonine protein kinase; inhibits pheromone induced signing downstream of MAPK, possibly at the level of the Ste12p transcription factor; mutant has increased aneuploidy tolerance; PRR2 has a paralog, NPR1, that arose from the whole genome duplication |
| NOP6 |
YDL213C |
Nucleolar protein 6; rRNA-binding protein required for 40S ribosomal subunit biogenesis; contains an RNA recognition motif (RRM); hydrophilin essential to overcome the stress of the desiccation-rehydration process; NOP6 may be a fungal-specific gene as no homologs have been yet identified in higher eukaryotes |
| SHR3 |
YDL212W |
Secretory component protein SHR3; Endoplasmic reticulum packaging chaperone; required for incorporation of amino acid permeases into COPII coated vesicles for transport to the cell surface |
| YDL211C |
YDL211C |
Uncharacterized protein YDL211C; Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YDL211C has a paralog, TDA7, that arose from the whole genome duplication |
| UGA4 |
YDL210W |
GABA (gamma-aminobutyrate) permease; serves as a GABA transport protein involved in the utilization of GABA as a nitrogen source; catalyzes the transport of putrescine and delta-aminolevulinic acid (ALA); localized to the vacuolar membrane; Belongs to the amino acid-polyamine-organocation (APC) superfamily. Amino acid/choline transporter (ACT) (TC 2.A.3.4) family |
| CWC2 |
YDL209C |
Pre-mRNA-splicing factor CWC2; Member of the NineTeen Complex (NTC); this complex contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2; Belongs to the RRM CWC2 family |
| NHP2 |
YDL208W |
H/ACA ribonucleoprotein complex subunit 2; Protein related to mammalian high mobility group (HMG) proteins; nuclear protein; essential for function of H/ACA-type snoRNPs, which are involved in 18S rRNA processing; Belongs to the eukaryotic ribosomal protein eL8 family |
| GLE1 |
YDL207W |
Cytoplasmic nucleoporin required for polyadenylated mRNA export; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export; mediates translation initiation; required for efficient translation termination |
| YDL206W |
YDL206W |
Solute carrier family 24 (sodium/potassium/calcium exchanger), member 6; Putative protein of unknown function; YDL206W is not an essential protein |
| HEM3 |
YDL205C |
Porphobilinogen deaminase; catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme; human homolog HMBS can complement yeast mutant and ow growth of haploid null after sporulation of a heterozygous diploid |
| RTN2 |
YDL204W |
Reticulon-like protein 2; Reticulon protein; involved in nuclear pore assembly and maintenance of tubular ER morphology; promotes membrane curvature; regulates the ER asymmetry-induced inheritance block during ER stress; role in ER-derived peroxisomal biogenesis; interacts with Sec6p, Yip3p, and Sbh1p; less abundant than RTN1; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress |
| ACK1 |
YDL203C |
Activator of C kinase protein 1; Protein that functions in the cell w integrity pathway; functions upstream of Pkc1p; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria |
| MRPL11 |
YDL202W |
Mitochondrial 54s ribosomal protein yml11; Mitochondrial ribosomal protein of the large subunit; localizes to vacuole in response to H2O2 |
| TRM8 |
YDL201W |
Noncatalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p |
| MGT1 |
YDL200C |
Methylated-dna-[protein]-cysteine s-methyltransferase; DNA repair methyltransferase (6-O-methylguanine-DNA methylase); involved in protection against DNA alkylation damage; Belongs to the MGMT family |
| YDL199C |
YDL199C |
Putative metabolite transport protein YDL199C; Putative transporter; member of the sugar porter family; Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family |
| GGC1 |
YDL198C |
Mitochondrial GTP/GDP carrier protein 1; Mitochondrial GTP/GDP transporter; essential for mitochondrial genome maintenance; has a role in mitochondrial iron transport; member of the mitochondrial carrier family |
| ASF2 |
YDL197C |
Anti-silencing protein 2; Anti-silencing protein; causes derepression of silent loci when overexpressed |
| SEC31 |
YDL195W |
Protein transport protein SEC31; Component of the Sec13p-Sec31p complex of the COPII vesicle coat; COPII coat is required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance |
| SNF3 |
YDL194W |
Mfs transporter, sp family, sugar:h+ symporter; High-affinity glucose transporter SNF3; Plasma membrane low glucose sensor, regulates glucose transport; high affinity sensor that contains 12 predicted transmembrane segments and a long C-terminal tail required for induction of hexose transporters; also senses fructose and mannose; SNF3 has a paralog, RGT2, that arose from the whole genome duplication |
| NUS1 |
YDL193W |
Forms dehydrodolichyl diphosphate syntase complex with RER2 or SRT1; tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY, suggesting that Nus1p may be involved in protein trafficking; mutations in human homolog NUS1 have been implicated in congenital scoliosis, neurological impairment, refractory epilepsy, hearing deficit, and visual impairment; human cis-prenyltransferase complex complements yeast null mutant |
| ARF1 |
YDL192W |
ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication; arf1 arf2 double null mutation is complemented by human ARF1, ARF4, ARF5, or ARF6 |
| RPL35A |
YDL191W |
Ribosomal 60S subunit protein L35A; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35A has a paralog, RPL35B, that arose from the whole genome duplication |
| UFD2 |
YDL190C |
E4 ubiquitin-protein ligase UFD2; Ubiquitin chain assembly factor (E4); cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3 |
| RBS1 |
YDL189W |
RNA-binding suppressor of PAS kinase protein 1; Protein involved in assembly of the RNA polymerase III (Pol III) complex; high copy suppressor of Pol III assembly mutation and psk1 psk2 mutations that confer temperature-sensitivity for galactose utilization; physicy interacts with Pol III; proposed to bind single-stranded nucleic acids via its R3H domain |
| PPH22 |
YDL188C |
Catalytic subunit of protein phosphatase 2A (PP2A); functiony redundant with Pph21p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; protein abundance increases in response to DNA replication stress; dephosphorylates Tel1p/Mec1p-phosphorylated Cdc13p to promote telomerase release from telomeres at G2/M; PPH22 has a paralog, PPH21, that arose from the whole genome duplication |
| YDL186W |
YDL186W |
Uncharacterized protein ydl186w; Putative protein of unknown function; YDL186W is not an essential gene |
| VMA1 |
YDL185W |
Subunit A of the V1 peripheral membrane domain of V-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease; the V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase) has eight subunits; involved in methionine restriction extension of chronological lifespan in an autophagy-dependent manner; Belongs to the ATPase alpha/beta chains family |
| RPL41A |
YDL184C |
Ribosomal 60S subunit protein L41A; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41A has a paralog, RPL41B, that arose from the whole genome duplication |
| MRX19 |
YDL183C |
Uncharacterized protein YDL183C; Protein that may form an active mitochondrial KHE system; mitochondrial inner-membrane protein; non-essential gene; KHE system stands for K+/H+ exchanger system; To S.pombe SpAC23H3.12c |
| LYS20 |
YDL182W |
Homocitrate synthase isozyme and functions in DNA repair; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS20 has a paralog, LYS21, that arose from the whole genome duplication; Belongs to the alpha-IPM synthase/homocitrate synthase family |
| INH1 |
YDL181W |
ATPase inhibitor, mitochondrial; Protein that inhibits ATP hydrolysis by the F1F0-ATP synthase; inhibitory function is enhanced by stabilizing proteins Stf1p and Stf2p; has a calmodulin-binding motif and binds calmodulin in vitro; INH1 has a paralog, STF1, that arose from the whole genome duplication |
| YDL180W |
YDL180W |
Uncharacterized membrane protein YDL180W; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole |
| PCL9 |
YDL179W |
PHO85 cyclin-9; Cyclin; forms a functional kinase complex with Pho85p cyclin-dependent kinase (Cdk), expressed in late M/early G1 phase, activated by Swi5p; PCL9 has a paralog, PCL2, that arose from the whole genome duplication; Belongs to the cyclin family. PCL1,2 subfamily |
| DLD2 |
YDL178W |
D-2-hydroxyglutarate--pyruvate transhydrogenase DLD2; D-2-hydroxyglutarate dehydrogenase, and minor D-lactate dehydrogenase; mitochondrial matrix protein that oxidizes D-2-hydroxyglutarate (D-2HG), an oncometabolite, to alpha-ketoglutarate with a minor role in lactate catabolism; located in the mitochondrial matrix; Belongs to the FAD-binding oxidoreductase/transferase type 4 family |
| YDL177C |
YDL177C |
IMPACT family member YDL177C; Putative protein of unknown function; similar to the mouse IMPACT gene; YDL177C is not an essential gene |
| IPF1 |
YDL176W |
Uncharacterized protein YDL176W; Protein of unknown function; predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene |
| AIR2 |
YDL175C |
Protein AIR2; RNA-binding subunit of the TRAMP nuclear RNA surveillance complex; involved in nuclear RNA processing and degradation; involved in TRAMP complex assembly as a bridge between Mtr4p and Trf4p; stimulates the poly(A) polymerase activity of Pap2p in vitro; has 5 zinc knuckle motifs; AIR2 has a paralog, AIR1, that arose from the whole genome duplication; Air2p and Air1p have nonredundant roles in regulation of substrate specificity of the exosome |
| DLD1 |
YDL174C |
Major mitochondrial D-lactate dehydrogenase; oxidizes D-lactate to pyruvate, transcription is heme-dependent, repressed by glucose, and derepressed in ethanol or lactate; located in the mitochondrial inner membrane |
| PAR32 |
YDL173W |
Protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene |
| GLT1 |
YDL171C |
NAD(+)-dependent glutamate synthase (GOGAT); synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source; assembles into filaments as cells approach stationary phase and under cytosolic acidification and starvation conditions |
| UGA3 |
YDL170W |
Transcriptional activator for GABA-dependent induction of GABA genes; binds to DNA elements found in the promoters of target genes and increases their expression in the presence of GABA (gamma-aminobutyrate); zinc finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus; examples of GABA genes include UGA1, UGA2, and UGA4 |
| UGX2 |
YDL169C |
Protein ugx2; Protein of unknown function; transcript accumulates in response to any combination of stress conditions |
| SFA1 |
YDL168W |
Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress; Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily |
| NRP1 |
YDL167C |
Putative RNA binding protein of unknown function; localizes to stress granules induced by glucose deprivation; predicted to be involved in ribosome biogenesis |
| FAP7 |
YDL166C |
Adenylate kinase isoenzyme 6 homolog FAP7; Essential NTPase required for sm ribosome subunit synthesis; mediates processing of the 20S pre-rRNA at site D in the cytoplasm but associates only transiently with 43S preribosomes via Rps14p; complex with Rps14 is conserved between humans, yeast, and arches; may be the endonuclease for site D; depletion leads to accumulation of pre-40S ribosomes in 80S-like ribosomes; human TAF9 functiony complements the lethality of the null mutation |
| CDC36 |
YDL165W |
General negative regulator of transcription subunit 2; Component of the CCR4-NOT core complex, involved in mRNA decapping; this complex has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs through deadenylation; basal transcription factor |
| CDC9 |
YDL164C |
DNA ligase I found in nucleus and mitochondria; essential enzyme that joins Okazaki fragments during DNA replication; also acts in ribonucleotide excision repair, base excision repair, and recombination; DNA ligase I mutants trigger ubiquitination of PCNA at K107, facilitating Rad59p-mediated bypass of unligated Okazaki fragments; human homolog LIG1 can complement yeast cdc9 temperature-sensitive mutant at restrictive temperature; Belongs to the ATP-dependent DNA ligase family |
| ENT1 |
YDL161W |
Epsin-1; Epsin-like protein involved in endocytosis and actin patch assembly; functiony redundant with Ent2p; binds clathrin via a clathrin-binding domain motif at C-terminus; relocalizes from bud neck to cytoplasm upon DNA replication stress; ENT1 has a paralog, ENT2, that arose from the whole genome duplication |
| MHF2 |
YDL160C-A |
Component of the heterotetrameric MHF histone-fold complex; in humans the MHF complex interacts with both DNA and Mph1p ortholog FANCM to stabilize and remodel blocked replication forks and repair damaged DNA; mhf2 srs2 double mutants are MMS hypersensitive; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-X, also known as MHF2 |
| DHH1 |
YDL160C |
Cytoplasmic DEAD-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacting with both decapping and deadenylase complexes; role in translational repression, mRNA decay, and possibly mRNA export; interacts and cooperates with Ngr1p to promote specific mRNA decay; ATP- and RNA-bound form promotes processing body (PB) assembly, while ATPase stimulation by Not1p promotes PB disassembly; forms cytoplasmic foci on replication stress; Belongs to the DEAD box helicase family. DDX6/DHH1 subfamily |
| YDL159W-A |
YDL159W-A |
Uncharacterized protein YDL159W-A; Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species |
| STE7 |
YDL159W |
Serine/threonine-protein kinase STE7; Signal transducing MAP kinase kinase; involved in pheromone response where it phosphorylates Fus3p; involved in the pseudohyphal/invasive growth pathway where it phosphorylates of Kss1p; phosphorylated by Ste11p; degraded by ubiquitin pathway |
| YDL157C |
YDL157C |
Uncharacterized protein YDL157C, mitochondrial; Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; To S.pombe tam6 |
| CMR1 |
YDL156W |
DNA damage-binding protein CMR1; Nuclear protein with a role in protein quality control; localizes to the intranuclear quality control compartment (INQ) in response to proteasome inhibition or DNA replication stress; INQ likely sequesters proteins involved in DNA metabolism for degradation or re-folding; DNA-binding protein with preference for UV-damaged DNA; contains three WD domains (WD-40 repeat); human ortholog WDR76 also exhibits perinuclear localization under similar stress conditions |
| CLB3 |
YDL155W |
G2/mitotic-specific cyclin-3; B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; relative distribution to the nucleus increases upon DNA replication stress; CLB3 has a paralog, CLB4, that arose from the whole genome duplication |
| MSH5 |
YDL154W |
Protein of the MutS family; forms a dimer with Msh4p that facilitates crossovers between homologs during meiosis; msh5-Y823H mutation confers tolerance to DNA alkylating agents; homologs present in C. elegans and humans |
| SAS10 |
YDL153C |
Something about silencing protein 10; Subunit of U3-containing Sm Subunit (SSU) processome complex; involved in production of 18S rRNA and assembly of sm ribosomal subunit; disrupts silencing when overproduced; mutant has increased aneuploidy tolerance; essential gene |
| RPC53 |
YDL150W |
RNA polymerase III subunit C53; Belongs to the eukaryotic RPC4/POLR3D RNA polymerase subunit family |
| ATG9 |
YDL149W |
Autophagy-related protein 9; Transmembrane protein involved in forming Cvt and autophagic vesicles; cycles between the phagophore assembly site (PAS) and other cytosolic punctate structures, not found in autophagosomes; may be involved in membrane delivery to the PAS; Belongs to the ATG9 family |
| NOP14 |
YDL148C |
Nucleolar protein; forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the sm subunit processome complex, which is required for processing of pre-18S rRNA; Belongs to the NOP14 family |
| RPN5 |
YDL147W |
Subunit of the CSN and 26S proteasome lid complexes; similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein; the COP9 signalosome is also known as the CSN |
| LDB17 |
YDL146W |
Protein involved in the regulation of endocytosis; transiently recruited to actin cortical patches in a SLA1-dependent manner after late coat component assembly; GFP-fusion protein localizes to the periphery, cytoplasm, bud, and bud neck; Belongs to the LDB17 family |
| COP1 |
YDL145C |
Alpha subunit of COPI vesicle coatomer complex; complex surrounds transport vesicles in the early secretory pathway |
| YDL144C |
YDL144C |
Uncharacterized protein YDL144C; Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL144C is not an essential gene; protein abundance increases in response to DNA replication stress |
| CCT4 |
YDL143W |
T-complex protein 1 subunit delta; Subunit of the cytosolic chaperonin Cct ring complex; related to Tcp1p, required for the assembly of actin and tubulins in vivo |
| CRD1 |
YDL142C |
Cardiolipin synthase; produces cardiolipin, which is a phospholipid of the mitochondrial inner membrane that is required for normal mitochondrial membrane potential and function and for correct integration of membrane-multispanning proteins into the mitochondrial outer membrane; required to maintain tubular mitochondrial morphology and functions in mitochondrial fusion; also required for normal vacuolar ion homeostasis; Belongs to the CDP-alcohol phosphatidyltransferase class-I family |
| BPL1 |
YDL141W |
Biotin:apoprotein ligase; covalently modifies proteins with the addition of biotin, required for acetyl-CoA carboxylase (Acc1p) holoenzyme formation; comparative analysis suggests that a mitochondriy targeted form may result from translation starting at a non-canonical codon upstream of the annotated start codon; human homolog HLCS can complement yeast BPL1 mutant |
| RPO21 |
YDL140C |
RNA polymerase II largest subunit B220; part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime |
| SCM3 |
YDL139C |
Protein SCM3; Nonhistone component of centromeric chromatin; binds to histone H3 variant, Cse4p, and recruits it to centromeres; involved in the assembly and maintenance of Cse4-H4 at centromeres; required for kinetochore assembly and G2/M progression; may protect Cse4p from ubiquitination; homolog of mammalian HJURP |
| RGT2 |
YDL138W |
Mfs transporter, sp family, sugar:h+ symporter; High-affinity glucose transporter RGT2; Plasma membrane high glucose sensor that regulates glucose transport; low affinity sesnor that contains 12 predicted transmembrane segments and a long C-terminal tail required for hexose transporter induction; phosphorylation of the tail by Yck1p/Yck2p facilitates binding to the HXT co-repressors, Mth1p and Std1p; RGT2 has a paralog, SNF3, that arose from the whole genome duplication |
| ARF2 |
YDL137W |
ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; ARF2 has a paralog, ARF1, that arose from the whole genome duplication; arf1 arf2 double null mutation is complemented by human ARF1, ARF4, ARF5, or ARF6 |
| RPL35B |
YDL136W |
Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication |
| RDI1 |
YDL135C |
Rho GDP dissociation inhibitor; involved in the localization and regulation of Cdc42p and Rho1p; protein abundance increases in response to DNA replication stress |
| PPH21 |
YDL134C |
Catalytic subunit of protein phosphatase 2A (PP2A); functiony redundant with Pph22p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; forms nuclear foci upon DNA replication stress; PPH21 has a paralog, PPH22, that arose from the whole genome duplication |
| RPL41B |
YDL133C-A |
Ribosomal 60S subunit protein L41B; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41B has a paralog, RPL41A, that arose from the whole genome duplication |
| SRF1 |
YDL133W |
Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneurony in Alzheimer's patients |
| CDC53 |
YDL132W |
Cell division control protein 53; Cullin; structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation; human homolog CUL1 can complement yeast cdc53 null mutant |
| LYS21 |
YDL131W |
Homocitrate synthase, mitochondrial; Homocitrate synthase isozyme; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS21 has a paralog, LYS20, that arose from the whole genome duplication; Belongs to the alpha-IPM synthase/homocitrate synthase family |
| STF1 |
YDL130W-A |
ATPase-stabilizing factor 9 kDa, mitochondrial; Protein involved in regulation of the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p act as stabilizing factors that enhance inhibitory action of the Inh1p protein; protein abundance increases in response to DNA replication stress; STF1 has a paralog, INH1, that arose from the whole genome duplication |
| RPP1B |
YDL130W |
60S acidic ribosomal protein P1-beta; Ribosomal protein P1 beta; component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; free (non-ribosomal) P1 stimulates the phosphorylation of the eIF2 alpha subunit (Sui2p) by Gcn2p; accumulation is regulated by phosphorylation and interaction with the P2 stalk component |
| YDL129W |
YDL129W |
Uncharacterized protein YDL129W; Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; YDL129W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress |
| VCX1 |
YDL128W |
Vacuolar calcium ion transporter; Vacuolar membrane antiporter with Ca2+/H+ and K+/H+ exchange activity; involved in control of cytosolic Ca2+ and K+ concentrations; has similarity to sodium/calcium exchangers, including the bovine Na+/Ca2+,K+ antiporter |
| PCL2 |
YDL127W |
PHO85 cyclin-2; Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; localizes to sites of polarized cell growth; PCL2 has a paralog, PCL9, that arose from the whole genome duplication |
| CDC48 |
YDL126C |
Transitional endoplasmic reticulum atpase; Cell division control protein 48; AAA ATPase; subunit of polyUb-selective segregase complex involved in ERAD, INM-associated degradation (INMAD), mitotic spindle disassembly, macroautophagy, PMN, ribosome-associated degradation, ribophagy, homotypic ER membrane fusion, SCF complex disassembly, cell w integrity during heat stress, and telomerase regulation; mobilizes membrane-anchored transcription factors by regulated Ub/proteasome-dependent processing (RUP); human ortholog VCP complements a cdc48 mutant |
| HNT1 |
YDL125C |
Hit family protein 1; Adenosine 5'-monophosphoramidase; interacts physicy and geneticy with Kin28p, a CDK and TFIIK subunit, and geneticy with CAK1; member of histidine triad (HIT) superfamily of nucleotide-binding proteins; protein abundance increases in response to DNA replication stress; human homolog HINT1 can complement yeast hnt1 mutant |
| YDL124W |
YDL124W |
NADPH-dependent alpha-keto amide reductase; reduces aromatic alpha-keto amides, aliphatic alpha-keto esters, and aromatic alpha-keto esters; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress |
| SNA4 |
YDL123W |
Protein of unknown function; localized to the vacuolar outer membrane; predicted to be palmitoylated |
| UBP1 |
YDL122W |
Ubiquitin carboxyl-terminal hydrolase 1; Ubiquitin-specific protease; removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains |
| EXP1 |
YDL121C |
Uncharacterized protein YDL121C; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDL121C is not an essential protein |
| YFH1 |
YDL120W |
Frataxin homolog, mitochondrial; Mitochondrial matrix iron chaperone; oxidizes and stores iron; interacts with Isu1p to promote Fe-S cluster assembly; mutation results in multiple Fe/S-dependent enzyme deficiencies; human frataxin homolog FXN is mutated in Friedrich's ataxia; human FTL gene can complement yeast yfh1 null mutant |
| HEM25 |
YDL119C |
Solute carrier family 25 member 38 homolog; Mitochondrial glycine transporter; required for the transport of glycine into mitochondria for initiation of heme biosynthesis, with YMC1 acting as a secondary transporter; homolog of human SLC25A38, a mitochondrial glycine transporter associated with nonsyndromic autosomal recessive congenital sideroblastic anemia; human SLC25A38 can complement the heme deficiency associated with the null mutant; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; Belongs to the mitochondrial carrier (TC 2.A.29) family. SLC25A38 subfamily |
| CYK3 |
YDL117W |
SH3-domain protein located in the bud neck and cytokinetic actin ring; relocalizes from bud neck to nucleus upon DNA replication stress; activates the chitin synthase activity of Chs2p during cytokinesis; suppressor of growth and cytokinesis defects of chs2 phospho-mutants |
| NUP84 |
YDL116W |
Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP107 |
| IWR1 |
YDL115C |
RNA polymerase II nuclear localization protein IWR1; RNA polymerase II transport factor, conserved from yeast to humans; also has a role in transporting RNA polymerase III into the nucleus; interacts with most of the RNAP II subunits; nucleo-cytoplasmic shuttling protein; deletion causes hypersensitivity to K1 killer toxin; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress |
| YDL114W |
YDL114W |
Uncharacterized oxidoreductase YDL114W; Putative short-chain dehydrogenase/reductase; YDL114W is not an essential gene; Belongs to the short-chain dehydrogenases/reductases (SDR) family |
| ATG20 |
YDL113C |
Autophagy-related protein 20; Sorting nexin family member; required for the cytoplasm-to-vacuole targeting (Cvt) pathway and for endosomal sorting; has a Phox homology domain that binds phosphatidylinositol-3-phosphate; interacts with Snx4p; potential Cdc28p substrate |
| TRM3 |
YDL112W |
tRNA (guanosine(18)-2'-O)-methyltransferase; 2'-O-ribose methyltransferase; catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs; Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family |
| RRP42 |
YDL111C |
Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7) |
| TMA17 |
YDL110C |
Translation machinery-associated protein 17; ATPase dedicated chaperone that adapts proteasome assembly to stress; Tma17p is induced upon stress; interacts with Rpt6p to assist its pairing to Rpt3p and early steps in proteasome biogenesis; associates with ribosomes; heterozygous deletion demonstrated increases in chromosome instability in a rad9 deletion background; protein abundance is decreased upon intracellular iron depletion |
| YDL109C |
YDL109C |
Putative lipase; involved in lipid metabolism; not an essential gene; YDL109C has a paralog, ROG1, that arose from the whole genome duplication |
| KIN28 |
YDL108W |
Serine/threonine-protein kinase KIN28; Serine/threonine protein kinase, subunit of transcription factor TFIIH; involved in transcription initiation at RNA polymerase II promoters; phosphorylates Ser5 residue of the PolII C-terminal domain (CTD) at gene promoters; relocalizes to the cytosol in response to hypoxia |
| MSS2 |
YDL107W |
Protein MSS2, mitochondrial; Periphery bound inner membrane protein of the mitochondrial matrix; involved in membrane insertion of C-terminus of Cox2p, interacts geneticy and physicy with Cox18p |
| PHO2 |
YDL106C |
Regulatory protein PHO2; Homeobox transcription factor; regulatory targets include genes involved in phosphate metabolism; binds cooperatively with Pho4p to the PHO5 promoter; phosphorylation of Pho2p facilitates interaction with Pho4p; relocalizes to the cytosol in response to hypoxia |
| NSE4 |
YDL105W |
Non-structural maintenance of chromosome element 4; Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; Belongs to the NSE4 family |
| QRI7 |
YDL104C |
tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial; Protein involved in threonylcarbamoyl adenosine biosynthesis; Sua5p and Qri7p are necessary and sufficient for RNA t6A modification in vitro; highly conserved mitochondrial protein; essential for t6A modification of mitochondrial tRNAs that decode ANN codons; similar to Kae1p and E. coli YgjD, both of which are also required for tRNA t6A modification; when directed to the cytoplasm, complements the essential function of Kae1p in the KEOPS complex |
| QRI1 |
YDL103C |
Udp-n-acetylglucosamine/udp-n-acetylgalactosamine diphosphorylase; UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell w biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress; Belongs to the UDPGP type 1 family |
| POL3 |
YDL102W |
Dna-directed dna polymerase delta pol3; Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER) |
| DUN1 |
YDL101C |
DNA damage response protein kinase DUN1; Cell-cycle checkpoint S/T protein kinase; required for transient G2/M arrest after DNA damage, damage-induced transcription, and nuclear-to-cytoplasmic redistribution of Rnr2p-Rnr4p after genotoxic stress and iron deprivation; phosphorylates repair protein Rad55p, transcriptional repressor Sml1p, superoxide dismutase, and ribonucleotide reductase inhibitors Crt1p and Dif1p; functions in the Mec1p pathway to regulate dNTP pools and telomere length; postreplicative repair role |
| GET3 |
YDL100C |
ATPase GET3; Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; functions as a chaperone under ATP-depleted oxidative stress conditions; subunit of GET complex, involved in ATP dependent Golgi to ER trafficking and insertion of tail-anchored (TA) proteins into ER membrane under non-stress conditions; binds as dimer to transmembrane domain (TMD) cargo, shielding TMDs from aqueous solvent; protein abundance increases under DNA replication stress |
| BUG1 |
YDL099W |
Binder of USO1 and GRH1 protein 1; Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes |
| SNU23 |
YDL098C |
23 kDa U4/U6.U5 sm nuclear ribonucleoprotein component; Component of the U4/U6.U5 snRNP complex; involved in mRNA splicing via spliceosome |
| RPN6 |
YDL097C |
Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partiy rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress |
| PMT1 |
YDL095W |
Dolichyl-phosphate-mannose--protein mannosyltransferase 1; Protein O-mannosyltransferase of the ER membrane; transfers mannose from dolichyl phosphate-D-mannose to protein serine and threonine residues; 1 of 7 related proteins involved in O-glycosylation which is essential for cell w rigidity; involved in ER quality control; amino terminus faces cytoplasm, carboxyl terminus faces ER lumen |
| PMT5 |
YDL093W |
Putative dolichyl-phosphate-mannose-protein mannosyltransferase pmt5; Dolichyl-phosphate-mannose--protein mannosyltransferase 5; Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt3p, can instead interact with Pmt2p in some conditions; target for new antifungals; Belongs to the glycosyltransferase 39 family |
| SRP14 |
YDL092W |
Signal recognition particle (SRP) subunit; interacts with the RNA component of SRP to form the Alu domain, which is the region of SRP responsible for arrest of nascent chain elongation during membrane targeting; homolog of mammalian SRP14 |
| UBX3 |
YDL091C |
UBX domain-containing protein 3; Clathrin-coated vesicle component, regulator of endocytosis; copurifies with the DSC ubiquitin ligase complex; UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p; required for efficient clathrin-mediated endocytosis; ortholog of fission yeast Ucp10 |
| RAM1 |
YDL090C |
Beta subunit of the CAAX farnesyltransferase (FTase); this complex prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit |
| NUR1 |
YDL089W |
Nuclear rim protein 1; Protein involved in regulation of mitotic exit; dephosphorylation target of Cdc14p in anaphase, which promotes timely rDNA segregation and ows mitotic progression; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate; Belongs to the NUR1 family |
| ASM4 |
YDL088C |
FG-nucleoporin component of central core of nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport; induces membrane tubulation, which may contribute to nuclear pore assembly; ASM4 has a paralog, NUP53, that arose from the whole genome duplication |
| LUC7 |
YDL087C |
Essential protein associated with the U1 snRNP complex; splicing factor involved in recognition of 5' splice site; contains two zinc finger motifs; N-terminal zinc finger binds pre-mRNA; relocalizes to the cytosol in response to hypoxia; Belongs to the Luc7 family |
| YDL086W |
YDL086W |
Putative carboxymethylenebutenolidase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL086W is not an essential gene; Belongs to the dienelactone hydrolase family |
| YDL085C-A |
YDL085C-A |
SERF-like protein YDL085C-A; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; Belongs to the SERF family |
| NDE2 |
YDL085W |
External NADH-ubiquinone oxidoreductase 2, mitochondrial; Mitochondrial external NADH dehydrogenase; catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p are involved in providing the cytosolic NADH to the mitochondrial respiratory chain; NDE2 has a paralog, NDE1, that arose from the whole genome duplication |
| SUB2 |
YDL084W |
ATP-dependent RNA helicase SUB2; Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress |
| RPS16B |
YDL083C |
Protein component of the sm (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16B has a paralog, RPS16A, that arose from the whole genome duplication |
| RPL13A |
YDL082W |
Ribosomal 60S subunit protein L13A; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13A has a paralog, RPL13B, that arose from the whole genome duplication |
| RPP1A |
YDL081C |
60S acidic ribosomal protein P1-alpha; Ribosomal stalk protein P1 alpha; involved in the interaction between translational elongation factors and the ribosome; free (non-ribosomal) P1 stimulates the phosphorylation of the eIF2 alpha subunit (Sui2p) by Gcn2p; accumulation of P1 in the cytoplasm is regulated by phosphorylation and interaction with the P2 stalk component |
| THI3 |
YDL080C |
Thiamine metabolism regulatory protein THI3; Regulatory protein that binds Pdc2p and Thi2p transcription factors; activates thiamine biosynthesis transcription factors Pdc2p and Thi2p by binding to them, but releases and de-activates them upon binding to thiamine pyrophosphate (TPP), the end product of the pathway; has similarity to decarboxylases but enzymatic activity is not detected |
| MRK1 |
YDL079C |
Serine/threonine-protein kinase MRK1; Glycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation; MRK1 has a paralog, RIM11, that arose from the whole genome duplication |
| MDH3 |
YDL078C |
Peroxisomal malate dehydrogenase; catalyzes interconversion of malate and oxaloacetate; involved in the glyoxylate cycle |
| VAM6 |
YDL077C |
Vacuolar morphogenesis protein 6; Guanine nucleotide exchange factor for the GTPase Gtr1p; subunit of the HOPS endocytic tethering complex; vacuole membrane protein; functions as a Rab GTPase effector, interacting with both GTP- and GDP-bound conformations of Ypt7p; facilitates tethering and promotes membrane fusion events at the late endosome and vacuole; required for both membrane and protein trafficking; component of vacuole-mitochondrion contacts (vCLAMPs) important for lipid transfer between organelles |
| RXT3 |
YDL076C |
Transcriptional regulatory protein RXT3; Component of the Rpd3L histone deacetylase complex; involved in histone deacetylation; protein abundance increases in response to DNA replication stress; Belongs to the RXT3 family |
| RPL31A |
YDL075W |
Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication |
| SNR63 |
YNCD0002C |
Unknown |
| BRE1 |
YDL074C |
E3 ubiquitin ligase; forms heterodimer with Rad6p to regulate K63 polyubiquitination in response to oxidative stress and to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control; interacts with RNA-binding protein Npl3p, linking histone ubiquitination to mRNA processing; Bre1p-dependent histone ubiquitination promotes pre-mRNA splicing |
| AHK1 |
YDL073W |
UPF0592 protein YDL073W; Scaffold protein in the HKR1 sub-branch of the Hog1p-signaling pathway; physicy interacts with the cytoplasmic domain of Hkr1p, and with Sho1p, Pbs2p, and Ste11p; prevents cross-talk signaling from Hkr1p of the osmotic stress MAPK cascade to the Kss1p MAPK cascade; non-essential gene |
| YET3 |
YDL072C |
Endoplasmic reticulum transmembrane protein 3; Protein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein; protein abundance increases in response to DNA replication stress |
| BDF2 |
YDL070W |
Bromodomain-containing factor 2; Protein involved in transcription initiation; acts at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf1p; protein abundance increases in response to DNA replication stress; BDF2 has a paralog, BDF1, that arose from the whole genome duplication |
| CBS1 |
YDL069C |
Cytochrome b translational activator protein cbs1, mitochondrial; Mitochondrial translational activator of the COB mRNA; membrane protein that interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader |
| COX9 |
YDL067C |
Subunit VIIa of cytochrome c oxidase (Complex IV); Complex IV is the terminal member of the mitochondrial inner membrane electron transport chain |
| IDP1 |
YDL066W |
Mitochondrial NADP-specific isocitrate dehydrogenase; catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes |
| PEX19 |
YDL065C |
Chaperone and import receptor for newly-synthesized class I PMPs; binds peroxisomal membrane proteins (PMPs) in the cytoplasm and delivers them to the peroxisome for subsequent insertion into the peroxisomal membrane; interacts with Myo2p and contributes to peroxisome partitioning |
| UBC9 |
YDL064W |
SUMO-conjugating enzyme involved in the Smt3p conjugation pathway; nuclear protein required for S- and M-phase cyclin degradation and mitotic control; involved in proteolysis mediated by the anaphase-promoting complex cyclosome (APCC) |
| SYO1 |
YDL063C |
Synchronized import protein 1; Transport adaptor or symportin; facilitates synchronized nuclear coimport of the two 5S-rRNA binding proteins Rpl5p and Rpl11p; binds to nascent Rpl5p during translation; required for biogenesis of the large ribosomal subunit; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus |
| RPS29B |
YDL061C |
Protein component of the sm (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29B has a paralog, RPS29A, that arose from the whole genome duplication |
| TSR1 |
YDL060W |
Ribosome biogenesis protein TSR1; Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress; Belongs to the TRAFAC class translation factor GTPase superfamily. Bms1-like GTPase family. TSR1 subfamily |
| RAD59 |
YDL059C |
Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; regulates replication fork progression in DNA ligase I-deficient cells; paralog of Rad52p; Belongs to the RAD52 family |
| USO1 |
YDL058W |
Essential protein involved in vesicle-mediated ER to Golgi transport; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex |
| YDL057W |
YDL057W |
Putative uncharacterized protein ydl057w; Putative protein of unknown function; YDL057W is not an essential gene |
| MBP1 |
YDL056W |
Transcription factor mbp1; Transcription factor; involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes |
| PSA1 |
YDL055C |
GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase); synthesizes GDP-mannose from GTP and mannose-1-phosphate in cell w biosynthesis; required for normal cell w structure |
| YNCD0003W |
YNCD0003W |
Unknown |
| MCH1 |
YDL054C |
Probable transporter MCH1; Protein with similarity to mammalian monocarboxylate permeases; monocarboxylate permeases are involved in transport of monocarboxylic acids across the plasma membrane but mutant is not deficient in monocarboxylate transport |
| PBP4 |
YDL053C |
Pbp1p binding protein; interacts strongly with Pab1p-binding protein 1 (Pbp1p) in the yeast two-hybrid system; also interacts with Lsm12p in a copurification assay; relative distribution to the nucleus increases upon DNA replication stress |
| SLC1 |
YDL052C |
1-acyl-sn-glycerol-3-phosphate acyltransferase; catalyzes the acylation of lysophosphatidic acid to form phosphatidic acid, a key intermediate in lipid metabolism; enzymatic activity detected in lipid particles and microsomes |
| LHP1 |
YDL051W |
La protein homolog; RNA binding protein required for maturation of tRNA and U6 snRNA; acts as a molecular chaperone for RNAs transcribed by polymerase III; homologous to human La (SS-B) autoantigen |
| KNH1 |
YDL049C |
Protein with similarity to Kre9p; Kre9p is involved in cell w beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance |
| STP4 |
YDL048C |
Protein containing a Kruppel-type zinc-finger domain; similar to Stp1p, Stp2p; predicted transcription factor; relative distribution to the nucleus increases upon DNA replication stress; STP4 has a paralog, STP3, that arose from the whole genome duplication |
| SIT4 |
YDL047W |
Serine/threonine-protein phosphatase PP1-1; Ceramide-activated, type 2A-related serine-threonine phosphatase; functions in G1/S transition of mitotic cycle; controls lifespan, mitochondrial function, cell cycle progression by regulating HXK2 phosphorylation; regulator of COPII coat dephosphorylation; required for ER to Golgi traffic; interacts with Hrr25p kinase; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell w and actin cytoskeleton organization; similar to human PP6; Belongs to the PPP phosphatase family. PP-6 (PP-V) subfamily |
| NPC2 |
YDL046W |
Phosphatidylglycerol/phosphatidylinositol transfer protein; Sterol transport protein and functional homolog of human NPC2/He1; human NPC2 is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 disease involving retention of cholesterol in lysosomes; yeast NPC2 can complement mutations in human NPC2; Belongs to the NPC2 family |
| MRP10 |
YDL045W-A |
Mitochondrial ribosomal protein of the sm subunit; contains twin cysteine-x9-cysteine motifs; oxidized by Mia40p during import into mitochondria |
| FAD1 |
YDL045C |
Fmn adenylyltransferase; FAD synthase; Flavin adenine dinucleotide (FAD) synthetase; performs the second step in synthesis of FAD from riboflavin; mutation is functiony complemented by human FLAD1 |
| MTF2 |
YDL044C |
Mitochondrial transcription factor 2; Mitochondrial protein that interacts with mitochondrial RNA polymerase; interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription |
| PRP11 |
YDL043C |
Pre-mRNA-splicing factor PRP11; Subunit of the SF3a splicing factor complex; required for spliceosome assembly |
| SIR2 |
YDL042C |
Conserved NAD+ dependent histone deacetylase of the Sirtuin family; deacetylation targets are primarily nuclear proteins; required for telomere hypercluster formation in quiescent yeast cells; involved in regulation of lifespan; plays roles in silencing at HML, HMR, telomeres, and rDNA; negatively regulates initiation of DNA replication; functions as regulator of autophagy like mammalian homolog SIRT1, and also of mitophagy |
| NAT1 |
YDL040C |
Subunit of protein N-terminal acetyltransferase NatA; NatA comprised of Nat1p, Ard1p, and Nat5p; N-terminy acetylates many proteins to influence multiple processes such as cell cycle progression, heat-shock resistance, mating, sporulation, telomeric silencing and early stages of mitophagy; orthologous to human NAA15; expression of both human NAA10 and NAA15 functiony complements ard1 nat1 double mutant although single mutations are not complemented by their orthologs |
| PRM7 |
YDL039C |
Pheromone-regulated protein; predicted to have one transmembrane segment; promoter contains Gcn4p binding elements; in W303 strain one continuous open reading frame comprising of YDL037C, the intergenic region and YDL039C encodes the IMI1 |
| BSC1 |
YDL037C |
Bypass of stop codon protein 1; Protein of unconfirmed function; similar to cell surface flocculin Flo11p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; in W303 strain one continuous open reading frame comprising of YDL037C, the intergenic region and YDL039C encodes the gene IMI1 |
| PUS9 |
YDL036C |
Mitochondrial tRNA:pseudouridine synthase; catalyzes the formation of pseudouridine at position 32 in mitochondrial tRNAs; contains an N-terminal mitochondrial targeting sequence; PUS9 has a paralog, RIB2, that arose from the whole genome duplication |
| GPR1 |
YDL035C |
Plasma membrane G protein coupled receptor (GPCR); interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis |
| SLM3 |
YDL033C |
TRNA-5-taurinomethyluridine 2-sulfurtransferase; tRNA-specific 2-thiouridylase; responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human homolog TRMU is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF), and can complement yeast null mutant |
| DBP10 |
YDL031W |
Putative ATP-dependent RNA helicase of the DEAD-box protein family; constituent of 66S pre-ribosomal particles; essential protein involved in ribosome biogenesis; Belongs to the DEAD box helicase family. DDX54/DBP10 subfamily |
| PRP9 |
YDL030W |
Pre-mRNA-splicing factor PRP9; Subunit of the SF3a splicing factor complex; required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex; Belongs to the SF3A3 family |
| ARP2 |
YDL029W |
Actin-related protein 2; Essential component of the Arp2/3 complex; Arp2/3 is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity; required for efficient Golgi-to-ER trafficking in COPI mutants |
| MPS1 |
YDL028C |
Serine/threonine-protein kinase MPS1; Dual-specificity kinase; autophosphorylation required for function; required for spindle pole body (SPB) duplication and spindle checkpoint function; contributes to bi-orientation by promoting formation of force-generating kinetochore-microtubule attachments in meiosis I; substrates include SPB proteins Spc42p, Spc110p, and Spc98p, mitotic exit network protein Mob1p, kinetochore protein Cnn1p, and checkpoint protein Mad1p; substrate of APCC(Cdh1); similar to human Mps1p |
| MRX9 |
YDL027C |
MIOREX complex component 9; Protein that associates with mitochondrial ribosome; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL027C is not an essential gene |
| RTK1 |
YDL025C |
Probable serine/threonine-protein kinase RTK1; Putative protein kinase, potentiy phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress |
| DIA3 |
YDL024C |
Probable acid phosphatase DIA3; Protein of unknown function; involved in invasive and pseudohyphal growth |
| YDL022C-A |
YDL022C-A |
Uncharacterized protein YDL022C-A; Protein of unknown function; SWAT-GFP and mCherry fusion proteins localize to the cytosol; partiy overlaps the verified gene DIA3; identified by fungal homology and RT-PCR; mRNA identified as translated by ribosome profiling data |
| YNCD0004W |
YNCD0004W |
Unknown |
| GPD1 |
YDL022W |
NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases Ypk1p and Ypk2p, dephosphorylation increases catalytic activity; forms a heterodimer with Pnc1p to facilitate its peroxisomal import |
| GPM2 |
YDL021W |
Homolog of Gpm1p phosphoglycerate mutase; converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional; GPM2 has a paralog, GPM3, that arose from the whole genome duplication |
| RPN4 |
YDL020C |
Protein RPN4; Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptiony regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress |
| OSH2 |
YDL019C |
Member of an oxysterol-binding protein family with seven members; in S. cerevisiae, family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability; contains FFAT motif; interacts with ER anchor Scs2p at patches at the plasma membrane and at the nuclear envelope; regulated by sterol binding; OSH2 has a paralog, SWH1, that arose from the whole genome duplication |
| ERP3 |
YDL018C |
Protein erp3; Protein with similarity to Emp24p and Erv25p; member of the p24 family involved in ER to Golgi transport |
| CDC7 |
YDL017W |
Cell division control protein 7; DDK (Dbf4-dependent kinase) catalytic subunit; required for origin firing and replication fork progression in mitotic S phase through phosphorylation of Mcm2-7p complexes and Cdc45p; kinase activity correlates with cyclical DBF4 expression; required for pre-meiotic DNA replication, meiotic DSB formation, recruitment of monopolin complex to kinetochores during meiosis I, regulation of meiosis-specific Ndt80p; mutation complemented by human CDC7 and DBF4 co-expression; Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC7 subfamily |
| TSC13 |
YDL015C |
Trans-2-enoyl-CoA reductase (NADPH) TSC13; Very-long-chain enoyl-CoA reductase; Enoyl reductase; catalyzes last step in each cycle of very long chain fatty acid elongation; localizes to ER, highly enriched in a structure marking nuclear-vacuolar junctions; coimmunoprecipitates with elongases Elo2p and Elo3p; protein increases in abundance and relative distribution to ER foci increases upon DNA replication stress; human homolog TECR implicated in nonsyndromic mental retardation, can complement yeast mutant; Belongs to the steroid 5-alpha reductase family |
| NOP1 |
YDL014W |
rRNA 2'-O-methyltransferase fibrillarin; Histone glutamine methyltransferase, modifies H2A at Q105 in nucleolus; component of the sm subunit processome complex, which is required for processing of pre-18S rRNA; ortholog of mammalian fibrillarin; inviability of the null mutant is functiony complemented by human FBL |
| SLX5 |
YDL013W |
Subunit of the Slx5-Slx8 SUMO-targeted Ub ligase (STUbL) complex; role in Ub-mediated degradation of histone variant Cse4p preventing mislocalization to euchromatin; role in proteolysis of spindle positioning protein Kar9p, and DNA repair proteins Rad52p and Rad57p; forms SUMO-dependent nuclear foci, including DNA repair centers; contains a RING domain and two SIM motifs; associates with the centromere; required for maintenance of genome integrity like human ortholog RNF4 |
| YDL012C |
YDL012C |
Cysteine-rich and transmembrane domain-containing protein YDL012C; Tail-anchored plasma membrane protein with a conserved CYSTM module; possibly involved in response to stress; may contribute to non-homologous end-joining (NHEJ) based on ydl012c htz1 double null phenotype; YDL012C has a paralog, YBR016W, that arose from the whole genome duplication; Belongs to the CYSTM1 family |
| GRX6 |
YDL010W |
Cis-golgi localized monothiol glutaredoxin, binds Fe-S cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; GRX6 has a paralog, GRX7, that arose from the whole genome duplication |
| YDL009C |
YDL009C |
Uncharacterized protein YDL009C; Protein of unknown function; mRNA identified as translated by ribosome profiling data; SWAT-GFP and mCherry fusion proteins localize to the cytosol; partiy overlaps the verified ORF YDL010W; YDL009C is not an essential gene |
| APC11 |
YDL008W |
Catalytic core subunit, Anaphase-Promoting Complex/Cyclosome (APC/C); which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; contains a RING-H2 domain that is required for activity |
| YNCD0005C |
YNCD0005C |
Unknown |
| YDL007C-A |
YDL007C-A |
Putative uncharacterized protein ydl007c-a; Putative protein of unknown function |
| YNCD0006W |
YNCD0006W |
Unknown |
| RPT2 |
YDL007W |
ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle; N-myristoylation of Rpt2p at Gly2 is involved in regulating the proper intracellular distribution of proteasome activity by controlling the nuclear localization of the 26S proteasome |
| PTC1 |
YDL006W |
Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation; Belongs to the PP2C family |
| MED2 |
YDL005C |
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; relocalizes to the cytosol in response to hypoxia |
| ATP16 |
YDL004W |
Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; F1 translationy regulates ATP6 and ATP8 expression to achieve a balanced output of ATP synthase genes encoded in nucleus and mitochondria; phosphorylated |
| MCD1 |
YDL003W |
Sister chromatid cohesion protein 1; Essential alpha-kleisin subunit of the cohesin complex; required for sister chromatid cohesion in mitosis and meiosis; apoptosis induces cleavage and translocation of a C-terminal fragment to mitochondria; expression peaks in S phase |
| NHP10 |
YDL002C |
Non-histone protein 10; Non-essential INO80 chromatin remodeling complex subunit; preferentiy binds DNA ends, protecting them from exonucleatic cleavage; deletion affects telomere maintenance via recombination; related to mammalian high mobility group proteins |
| RMD1 |
YDL001W |
Sporulation protein rmd1; Required for sporulation where it is believed to have a role in meiotic nuclear division |
| NTH1 |
YDR001C |
Neutral trehalase, degrades trehalose; required for thermotolerance and may mediate resistance to other cellular stresses; phosphorylated and activated by Cdc28p at the G1/S phase transition to coordinately regulate carbohydrate metabolism and the cell cycle; inhibited by Dcs1p; NTH1 has a paralog, NTH2, that arose from the whole genome duplication; Belongs to the glycosyl hydrolase 37 family |
| YRB1 |
YDR002W |
Ran-specific GTPase-activating protein 1; Ran GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1 |
| RCR2 |
YDR003W |
Vacuolar protein; presumably functions within the endosomal-vacuolar trafficking pathway, affecting events that determine whether plasma membrane proteins are degraded or routed to the plasma membrane; RCR2 has a paralog, RCR1, that arose from the whole genome duplication |
| YDR003W-A |
YDR003W-A |
Uncharacterized protein YDR003W-A; Putative protein of unknown function; identified by expression profiling and mass spectrometry |
| RAD57 |
YDR004W |
Putative dna-dependent atpase rad57; DNA repair protein RAD57; Protein that stimulates strand exchange; stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p; Belongs to the RecA family |
| MAF1 |
YDR005C |
Highly conserved negative regulator of RNA polymerase III; involved in tRNA processing and stability; inhibits tRNA degradation via rapid tRNA decay (RTD) pathway; binds N-terminal domain of Rpc160p subunit of Pol III to prevent closed-complex formation; regulated by phosphorylation mediated by TORC1, protein kinase A, Sch9p, casein kinase 2; localizes to cytoplasm during vegetative growth and translocates to nucleus and nucleolus under stress conditions |
| SOK1 |
YDR006C |
Protein of unknown function; overexpression suppresses the growth defect of mutants lacking protein kinase A activity; involved in cAMP-mediated signaling; localized to the nucleus; similar to the mouse testis-specific protein PBS13 |
| TRP1 |
YDR007W |
Phosphoribosylanthranilate isomerase; catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA); enhances vegetative growth at low and high temperatures when used as an auxotrophic marker in strains such as W303 |
| GAL3 |
YDR009W |
Protein GAL3; Transcriptional regulator; involved in activation of the GAL genes in response to galactose; forms a complex with Gal80p to relieve Gal80p inhibition of Gal4p; binds galactose and ATP but does not have galactokinase activity; GAL3 has a paralog, GAL1, that arose from the whole genome duplication; Belongs to the GHMP kinase family. GalK subfamily |
| YDR010C |
YDR010C |
Uncharacterized protein YDR010C; Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data |
| SNQ2 |
YDR011W |
Protein SNQ2; Plasma membrane ATP-binding cassette (ABC) transporter; multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species |
| RPL4B |
YDR012W |
Ribosomal 60S subunit protein L4B; homologous to mammalian ribosomal protein L4 and bacterial L4; RPL4B has a paralog, RPL4A, that arose from the whole genome duplication |
| PSF1 |
YDR013W |
Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p); complex is localized to DNA replication origins and implicated in assembly of the DNA replication machinery |
| RAD61 |
YDR014W |
Protein RAD61; Subunit of a complex that inhibits sister chromatid cohesion; also negatively regulates chromosome condensation; inhibited by Eco1p-acetylated cohesin subunits Smc3p and Mcd1p; binds Smc3p ATPase head of cohesin; related to the human Wapl protein that controls the association of cohesin with chromatin |
| HED1 |
YDR014W-A |
Meiosis-specific protein; down-regulates Rad51p-mediated mitotic recombination when the meiotic recombination machinery is impaired; promotes synapsis and required for the normal morphogenesis of synaptonemal complex; prevents the recruitment of Rad54p to site-specific DNA double-strand breaks in vivo; early meiotic gene, transcribed specificy during meiotic prophase |
| DAD1 |
YDR016C |
Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis |
| KCS1 |
YDR017C |
Inositol-hexakisphosphate 5-kinase; Inositol hexakisphosphate and inositol heptakisphosphate kinase; generation of high energy inositol pyrophosphates by Kcs1p is required for many processes such as vacuolar biogenesis, stress response, RNA polymerase I-mediated rRNA transcription and telomere maintenance; inositol hexakisphosphate is also known as IP6; inositol heptakisphosphate is also known as IP7; Belongs to the inositol phosphokinase (IPK) family |
| YDR018C |
YDR018C |
Uncharacterized acyltransferase YDR018C; Probable membrane protein with three predicted transmembrane domains; similar to C. elegans F55A11.5 and maize 1-acyl-glycerol-3-phosphate acyltransferase; YDR018C has a paralog, CST26, that arose from the whole genome duplication |
| GCV1 |
YDR019C |
T subunit of the mitochondrial glycine decarboxylase complex; glycine decarboxylase is required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm |
| DAS2 |
YDR020C |
Putative uridine kinase DAS2; Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; weak similarity with uridine kinases and with phosphoribokinases; Belongs to the uridine kinase family |
| FAL1 |
YDR021W |
ATP-dependent RNA helicase FAL1; Nucleolar protein required for maturation of 18S rRNA; member of the eIF4A subfamily of DEAD-box ATP-dependent RNA helicases; 18S rRNA biogenesis defect of the null mutant is functiony complemented by human EIF4A3 |
| ATG31 |
YDR022C |
Autophagy-related protein 31; Autophagy-specific protein required for autophagosome formation; forms a complex with Atg17p and Atg29p that localizes other proteins to the pre-autophagosomal structure; constitutively phosphorylated, and phosphorylation of residue S174 is required for function; high-copy suppressor of CIK1 deletion |
| YNCD0007C |
YNCD0007C |
Unknown |
| SES1 |
YDR023W |
Serine--tRNA ligase, cytoplasmic; Cytosolic seryl-tRNA synthetase; class II aminoacyl-tRNA synthetase that aminoacylates tRNA(Ser), displays tRNA-dependent amino acid recognition which enhances discrimination of the serine substrate, interacts with peroxin Pex21p |
| RPS11A |
YDR025W |
Protein component of the sm (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; N-terminy propionylated in vivo; RPS11A has a paralog, RPS11B, that arose from the whole genome duplication |
| NSI1 |
YDR026C |
RNA polymerase I termination factor; binds to rDNA terminator element, required for efficient Pol I termination; required for rDNA silencing at NTS1; facilities association of Sir2p with NTS1, contributes to rDNA stability and cell longevity; interacts physicy with Fob1p and RENT subunits, Sir2p and Net1p; may interact with ribosomes, based on co-purification experiments; Myb-like DNA-binding protein; NSI1 has a paralog, REB1, that arose from the whole genome duplication |
| VPS54 |
YDR027C |
Vacuolar protein sorting-associated protein 54; Component of the GARP (Golgi-associated retrograde protein) complex; GARP is required for the recycling of proteins from endosomes to the late Golgi, and for mitosis after DNA damage induced checkpoint arrest; potentiy phosphorylated by Cdc28p; members of the GARP complex are Vps51p-Vps52p-Vps53p-Vps54p |
| REG1 |
YDR028C |
Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; REG1 has a paralog, REG2, that arose from the whole genome duplication |
| YDR029W |
YDR029W |
Uncharacterized protein YDR029W; Putative protein of unknown function; conserved among S. cerevisiae strains; YDR029W is not an essential gene |
| RAD28 |
YDR030C |
Radiation-sensitive protein 28; Protein involved in DNA repair; related to the human CSA protein that is involved in transcription-coupled repair nucleotide excision repair |
| MIX14 |
YDR031W |
Mitochondrial intermembrane space protein of unknown function; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress |
| PST2 |
YDR032C |
Protoplast secreted protein 2; Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication |
| MRH1 |
YDR033W |
Protein that localizes primarily to the plasma membrane; also found at the nuclear envelope; long-lived protein that is asymmetricy retained in the plasma membrane of mother cells; the authentic, non-tagged protein is detected in mitochondria in a phosphorylated state; null mutation confers sensitivity to acetic acid; Belongs to the archaeal/bacterial/fungal opsin family |
| LYS14 |
YDR034C |
Lysine biosynthesis regulatory protein LYS14; Transcriptional activator involved in regulating lysine biosynthesis; involved in the regulation of genes of the lysine biosynthesis pathway; requires 2-aminoadipate semialdehyde as co-inducer |
| YNCD0008W |
YNCD0008W |
Unknown |
| YNCD0009W |
YNCD0009W |
Unknown |
| YDR034W-B |
YDR034W-B |
Cysteine-rich and transmembrane domain-containing protein YDR034W-B; Predicted tail-anchored plasma membrane protein; contains conserved CYSTM module; related proteins in other organisms may be involved in response to stress; N- and C-terminal fusion proteins localize to the cell periphery; YDR034W-B has a paralog, YBR056W-A, that arose from the whole genome duplication; Belongs to the CYSTM1 family |
| ARO3 |
YDR035W |
Phospho-2-dehydro-3-deoxyheptonate aldolase, phenylalanine-inhibited; 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase; catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan |
| EHD3 |
YDR036C |
3-hydroxyisobutyryl-CoA hydrolase, mitochondrial; 3-hydroxyisobutyryl-CoA hydrolase; member of a family of enoyl-CoA hydratase/isomerases; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; phosphorylated; mutation affects fluid-phase endocytosis |
| KRS1 |
YDR037W |
Lysine--tRNA ligase, cytoplasmic; Lysyl-tRNA synthetase |
| ENA1 |
YDR040C |
Na(+)/li(+)-exporting p-type atpase ena1; P-type ATPase sodium pump; involved in Na+ and Li+ efflux to ow salt tolerance |
| RSM10 |
YDR041W |
Mitochondrial ribosomal protein of the sm subunit; has similarity to E. coli S10 ribosomal protein; essential for viability, unlike most other mitoribosomal proteins |
| YDR042C |
YDR042C |
Uncharacterized protein YDR042C; Putative protein of unknown function; expression is increased in ssu72-ts69 mutant |
| SNR47 |
YNCD0010C |
Unknown |
| NRG1 |
YDR043C |
Transcriptional regulator NRG1; Transcriptional repressor; recruits the Cyc8p-Tup1p complex to promoters; mediates glucose repression and negatively regulates a variety of processes including filamentous growth and alkaline pH response; activated in stochastic pulses of nuclear localization in response to low glucose |
| HEM13 |
YDR044W |
Oxygen-dependent coproporphyrinogen-III oxidase; Coproporphyrinogen III oxidase; oxygen-requiring enzyme that catalyzes sixth step in heme biosynthetic pathway; transcription is repressed by oxygen and heme (via Rox1p and Hap1p); human homolog CPOX can complement yeast mutant and ow growth of haploid null after sporulation of a heterozygous diploid; Belongs to the aerobic coproporphyrinogen-III oxidase family |
| RPC11 |
YDR045C |
RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS |
| BAP3 |
YDR046C |
Valine amino-acid permease; Amino acid permease; involved in uptake of cysteine, leucine, isoleucine and valine; BAP3 has a paralog, BAP2, that arose from the whole genome duplication |
| HEM12 |
YDR047W |
Uroporphyrinogen decarboxylase; catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda |
| VMS1 |
YDR049W |
Protein VMS1; Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondriy-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans; Belongs to the ANKZF1/VMS1 family |
| TPI1 |
YDR050C |
Triose phosphate isomerase, abundant glycolytic enzyme; mRNA half-life is regulated by iron availability; transcription is controlled by activators Reb1p, Gcr1p, and Rap1p through binding sites in the 5' non-coding region; inhibition of Tpi1p activity by PEP (phosphoenolpyruvate) stimulates redox metabolism in respiring cells; E104D mutation in human homolog TPI1 causes a rare autosomal disease; human TPI1 can complement yeast null mutant |
| DET1 |
YDR051C |
Acid phosphatase; involved in the non-vesicular transport of sterols in both directions between the endoplasmic reticulum and plasma membrane; deletion confers sensitivity to nickel; Belongs to the phosphoglycerate mutase family |
| DBF4 |
YDR052C |
Regulatory subunit of Cdc7p-Dbf4p kinase complex; required for Cdc7p kinase activity and initiation of DNA replication; phosphorylates the Mcm2-7 family of proteins; cell cycle regulated; relative distribution to the nucleus increases upon DNA replication stress; co-expression of human CDC7 and DBF4 complements single cdc7 or dbf4 null mutations or the cdc7 dbf4 double null mutation |
| CDC34 |
YDR054C |
Ubiquitin-conjugating enzyme (E2); catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation; protein abundance increases in response to DNA replication stress; human CDC34 functiony complements the thermosensitivity of the cdc34-2 mutant |
| PST1 |
YDR055W |
Cell w protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell w damage via disruption of FKS1; PST1 has a paralog, ECM33, that arose from the whole genome duplication; Belongs to the SPS2 family |
| EMC10 |
YDR056C |
Uncharacterized protein YDR056C; Putative protein of unknown function; subunit of evolutionarily conserved EMC (Endoplasmic Reticulum Membrane Complex) implicated in ERAD (ER-associated degradation) and proper assembly of multi-pass transmembrane (TM) proteins; EMC acts in yeast as an ER-mitochondria tether that interacts with outer membrane protein Tom5p of TOM (Translocase of the Mitochondrial Outer Membrane) complex; YDR056C is not an essential protein |
| YOS9 |
YDR057W |
Protein OS-9 homolog; ER quality-control lectin; integral subunit of the HRD ligase; participates in efficient ER retention of misfolded proteins by recognizing them and delivering them to Hrd1p; binds to glycans with terminal alpha-1,6 linked mannose on misfolded N-glycosylated proteins and participates in targeting proteins to ERAD; member of the OS-9 protein family |
| TGL2 |
YDR058C |
Lipase 2; Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli |
| YNCD0011W |
YNCD0011W |
Unknown |
| UBC5 |
YDR059C |
Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived, abnormal, or excess proteins, including histone H3; central component of the cellular stress response; expression is heat inducible; protein abundance increases in response to DNA replication stress; UBC5 has a paralog, UBC4, that arose from the whole genome duplication |
| MAK21 |
YDR060W |
Ribosome biogenesis protein MAK21; Constituent of 66S pre-ribosomal particles; required for large (60S) ribosomal subunit biogenesis; acts as part of a Mak21p-Noc2p-Rrp5p module that associates with nascent pre-rRNA during transcription and has a role in bigenesis of the large ribosomal subunit; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein |
| YDR061W |
YDR061W |
Protein with similarity to ABC transporter family members; lacks predicted membrane-spanning regions; transcriptiony activated by Yrm1p along with genes involved in multidrug resistance |
| LCB2 |
YDR062W |
Component of serine palmitoyltransferase; responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine; Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family |
| AIM7 |
YDR063W |
Protein that interacts with Arp2/3 complex; interacts with Arp2/3 complex to stimulate actin filament debranching and inhibit actin nucleation; has similarity to Cof1p and also to human glia maturation factor (GMF); null mutant displays elevated mitochondrial genome loss; Belongs to the actin-binding proteins ADF family. GMF subfamily |
| RPS13 |
YDR064W |
Protein component of the sm (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 |
| RRG1 |
YDR065W |
Protein of unknown function; required for vacuolar acidification and mitochondrial genome maintenance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; Belongs to the RRG1 family |
| RTR2 |
YDR066C |
RNA polymerase II subunit B1 CTD phosphatase RTR2; Protein of unknown function; exhibits genetic interactions with Rtr1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YDR066C is not an essential gene; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress; RTR2 has a paralog, RTR1, that arose from the whole genome duplication; Belongs to the RPAP2 family |
| OCA6 |
YDR067C |
Putative tyrosine-protein phosphatase OCA6; Cytoplasmic protein required for replication of Brome mosaic virus; S. cerevisiae is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT |
| DOS2 |
YDR068W |
Protein dos2; Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm |
| DOA4 |
YDR069C |
Ubiquitin hydrolase; deubiquitinates intralumenal vesicle (ILVs) cargo proteins; required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins destined for the vacuole; DOA4 has a paralog, UBP5, that arose from the whole genome duplication; Belongs to the peptidase C19 family |
| FMP16 |
YDR070C |
Protein FMP16, mitochondrial; Protein of unknown function; may be involved in responding to conditions of stress; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress |
| PAA1 |
YDR071C |
Polyamine N-acetyltransferase 1; Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication; Belongs to the acetyltransferase family. AANAT subfamily |
| IPT1 |
YDR072C |
Inositolphosphotransferase; involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin |
| SNF11 |
YDR073W |
Transcription regulatory protein SNF11; Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; interacts with a highly conserved 40-residue sequence of Snf2p; relocates to the cytosol under hypoxic conditions |
| TPS2 |
YDR074W |
Trehalose-phosphatase; Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in synthesis of the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress; In the N-terminal section; belongs to the glycosyltransferase 20 family |
| PPH3 |
YDR075W |
Catalytic subunit of protein phosphatase PP4 complex; Pph3p and Psy2p form active complex, Psy4p may provide substrate specificity; regulates recovery from the DNA damage checkpoint, the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair and efficient Non-Homologous End-Joining (NHEJ) pathway; involved in activation of Gln3p to eviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes; Belongs to the PPP phosphatase family. PP-4 (PP-X) subfamily |
| RAD55 |
YDR076W |
Putative dna-dependent atpase rad55; DNA repair protein RAD55; Protein that stimulates strand exchange; stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p; Belongs to the RecA family. RAD55 subfamily |
| SED1 |
YDR077W |
Cell w protein SED1; Major stress-induced structural GPI-cell w glycoprotein; associates with translating ribosomes, possible role in mitochondrial genome maintenance; ORF contains two distinct variable minisatellites; SED1 has a paralog, SPI1, that arose from the whole genome duplication |
| SHU2 |
YDR078C |
Suppressor of hydroxyurea sensitivity protein 2; Component of Shu complex (aka PCSS complex); Shu complex also includes Psy3, Csm2, Shu1, and promotes error-free DNA repair, Shu complex mediates inhibition of Srs2p function; promotes formation of Rad51p filaments; important for both mitotic and meiotic homologous recombination, and contains a conserved SWIM domain that is necessary for both |
| PET100 |
YDR079W |
Protein PET100, mitochondrial; Chaperone that facilitates the assembly of cytochrome c oxidase; integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme |
| TFB5 |
YDR079C-A |
Component of RNA polymerase II general transcription factor TFIIH; involved in transcription initiation and in nucleotide-excision repair; relocalizes to the cytosol in response to hypoxia; homolog of Chlamydomonas reinhardtii REX1-S protein involved in DNA repair; Belongs to the TFB5 family |
| VPS41 |
YDR080W |
Vacuolar protein sorting-associated protein 41; Subunit of the HOPS endocytic tethering complex; vacuole membrane protein that functions as a Rab GTPase effector, interacting specificy with the GTP-bound conformation of Ypt7p, facilitating tethering, docking and promoting membrane fusion events at the late endosome and vacuole; required for both membrane and protein trafficking; Yck3p-mediated phosphorylation regulates the organization of vacuolar fusion sites |
| PDC2 |
YDR081C |
Protein PDC2; Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions |
| STN1 |
YDR082W |
Protein STN1; Telomere end-binding and capping protein; plays a key role with Pol12p in linking telomerase action with completion of lagging strand synthesis, and in a regulatory step required for telomere capping; similar to human Stn1 |
| RRP8 |
YDR083W |
25S rRNA (adenine(645)-N(1))-methyltransferase; Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is syntheticy lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1; Belongs to the methyltransferase superfamily. RRP8 family |
| TVP23 |
YDR084C |
Integral membrane protein; localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; Belongs to the TVP23 family |
| AFR1 |
YDR085C |
Protein required for pheromone-induced projection (shmoo) formation; regulates septin architecture during mating; has an RVXF motif that mediates targeting of Glc7p to mating projections; interacts with Cdc12p; AFR1 has a paralog, YER158C, that arose from the whole genome duplication |
| SSS1 |
YDR086C |
Protein transport protein SSS1; Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p); this complex forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p; Belongs to the SecE/SEC61-gamma family |
| RRP1 |
YDR087C |
Ribosomal RNA-processing protein 1; Essential evolutionarily conserved nucleolar protein; necessary for biogenesis of 60S ribosomal subunits and for processing of pre-rRNAs to mature rRNA; associated with several distinct 66S pre-ribosomal particles; Belongs to the RRP1 family |
| SLU7 |
YDR088C |
Pre-mRNA-splicing factor SLU7; RNA splicing factor; required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain; Belongs to the SLU7 family |
| YNCD0013C |
YNCD0013C |
Unknown |
| VTC5 |
YDR089W |
Uncharacterized protein YDR089W; Novel subunit of the vacuolar transporter chaperone complex; vacuolar transmembrane protein that regulates biosynthesis of polyphosphate; deletion reduces and overexpression increases polyP accumulation; SPX domain (Syg1, Pho81, Xpr1)-containing protein involved in phosphate homeostasis; relocalizes from vacuole to cytoplasm upon DNA replication stress |
| ILT1 |
YDR090C |
Uncharacterized membrane protein YDR090C; Putative protein of unknown function |
| RLI1 |
YDR091C |
Translation initiation factor RLI1; Essential Fe-S protein; required for ribosome biogenesis, translation initiation/termination; facilitates binding of multifactor complex (MFC) of initiation factors to sm ribosomal subunit; Dom34-Hbs1 complex and Rli1p work in dissociating inactive ribosomes, thereby facilitating translation restart; forms complex with Lto1p and Yae1p; dependency on ROS-labile FeS clusters, activity in nuclear ribosomal-subunit export impaired by mild oxidative stress |
| UBC13 |
YDR092W |
E2 ubiquitin-conjugating enzyme; involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus |
| DNF2 |
YDR093W |
Phospholipid-transporting ATPase DNF2; Aminophospholipid translocase (flippase); type 4 P-type ATPase; involved in phospholipid translocation, contributing to endocytosis, protein transport, and cellular polarization; localizes primarily to the plasma membrane; localizes to the shmoo tip where it has a redundant role in the cellular response to mating pheromone; DNF2 has a paralog, DNF1, that arose from the whole genome duplication |
| GIS1 |
YDR096W |
Transcriptional activator/repressor GIS1; Histone demethylase and transcription factor; regulates genes during nutrient limitation; activity modulated by proteasome-mediated proteolysis; has JmjC and JmjN domain in N-terminus that interact, promoting stability and proper transcriptional activity; contains two transactivating domains downstream of Jmj domains and a C-terminal DNA binding domain; relocalizes to the cytosol in response to hypoxia; GIS1 has a paralog, RPH1, that arose from the whole genome duplication |
| MSH6 |
YDR097C |
Protein required for mismatch repair in mitosis and meiosis; forms a complex with Msh2p to repair both single-base & insertion-deletion mispairs; also involved in interstrand cross-link repair; potentiy phosphorylated by Cdc28p |
| GRX3 |
YDR098C |
Monothiol glutaredoxin-3; Glutathione-dependent oxidoreductase; hydroperoxide and superoxide-radical responsive; monothiol glutaredoxin subfamily member along with Grx4p and Grx5p; protects cells from oxidative damage; with Grx4p, binds to Aft1p in iron-replete conditions, promoting its dissociation from promoters; evidence exists indicating that the translation start site is not Met1 as currently annotated, but rather Met36; GRX3 has a paralog, GRX4, that arose from the whole genome duplication |
| YNCD0014C |
YNCD0014C |
Unknown |
| BMH2 |
YDR099W |
14-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress; abundance relative to Bmh1p increases during sporulation |
| TVP15 |
YDR100W |
Golgi apparatus membrane protein tvp15; Integral membrane protein; localized to late Golgi vesicles along with the v-SNARE Tlg2p |
| ARX1 |
YDR101C |
Probable metoprotease ARX1; Nuclear export factor for the ribosomal pre-60S subunit; shuttling factor which directly binds FG rich nucleoporins and facilities translocation through the nuclear pore complex; interacts directly with Alb1p; responsible for Tif6p recycling defects in the absence of Rei1; associated with the ribosomal export complex |
| YDR102C |
YDR102C |
Uncharacterized protein YDR102C; Putative protein of unknown function; conserved among S. cerevisiae strains; YDR102C is not an essential gene; homozygous diploid deletion strain exhibits high budding index |
| STE5 |
YDR103W |
Protein STE5; Pheromone-responsive MAPK scaffold protein; couples activation of the G-protein-coupled pheromone receptor to MAPK activation; intramolecular interaction of PH and VWA domains blocks activation of assembled signaling cascade components (Ste11p, Ste7p and Fus3p) under basal conditions; Gbeta-gamma (Ste4p-Ste18p)-dependent docking at the plasma membrane and binding of PI(4,5)P2 by the PH domain relieves autoinhibition, resulting in pheromone-dependent pathway activation |
| SPO71 |
YDR104C |
Sporulation-specific protein 71; Meiosis-specific protein required for prospore membrane morphogenesis; localizes to the prospore membrane (PSM) during sporulation; required for PSM elongation and closure; geneticy antagonistic to SPO1; recruits Vps13p to the PSM during sporulation; interacts and functions cooperatively with Spo73p; mutants have defects in the PSM, aberrant spore w formation and reduced PtdIns-phosphate pools in the PSM; contains three PH-like domains |
| TMS1 |
YDR105C |
Serine incorporator 1/3; Vacuolar membrane protein of unknown function; is conserved in mammals; predicted to contain eleven transmembrane helices; interacts with Pdr5p, a protein involved in multidrug resistance |
| ARP10 |
YDR106W |
Actin-like protein ARP10; Component of the dynactin complex; localized to the pointed end of the Arp1p filament; may regulate membrane association of the complex; Belongs to the actin family. ARP10 subfamily |
| YNCD0015C |
YNCD0015C |
Unknown |
| TMN2 |
YDR107C |
Transmembrane 9 superfamily member 2; Protein with a role in cellular adhesion and filamentous growth; similar to Tmn3p; member of the evolutionarily conserved Transmembrane Nine family of proteins with nine membrane-spanning segments; TMN2 has a paralog, EMP70, that arose from the whole genome duplication |
| TRS85 |
YDR108W |
Trafficking protein particle complex III-specific subunit 85; Component of transport protein particle (TRAPP) complex III; TRAPPIII is a multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating endosome-Golgi traffic and required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role |
| YDR109C |
YDR109C |
Uncharacterized sugar kinase YDR109C; Putative kinase; Belongs to the FGGY kinase family |
| FOB1 |
YDR110W |
Nucleolar protein that binds the rDNA replication fork barrier site; required for replication fork blocking, recombinational hotspot activity, condensin recruitment to replication fork barrier (RFB), and rDNA repeat segregation; related to retroviral integrases |
| ALT2 |
YDR111C |
Probable alanine aminotransferase; Catalyticy inactive alanine transaminase; expression is repressed in the presence of alanine and repression is mediated by Nrg1p; ALT2 has a paralog, ALT1, that arose from the whole genome duplication; Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily |
| PDS1 |
YDR113C |
Securin; inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; Belongs to the securin family |
| YDR114C |
YDR114C |
Uncharacterized protein YDR114C; Putative protein of unknown function; deletion mutant exhibits poor growth at elevated pH and calcium |
| MRX14 |
YDR115W |
Putative mitochondrial ribosomal protein of the large subunit; similar to E. coli L34 ribosomal protein; required for respiratory growth, as are most mitochondrial ribosomal proteins; protein increases in abundance and relocalizes to the plasma membrane upon DNA replication stress |
| MRPL1 |
YDR116C |
Mitochondrial 54s ribosomal protein mrpl1; Mitochondrial ribosomal protein of the large subunit |
| TMA64 |
YDR117C |
Translation machinery-associated protein 64; Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; in mammals the corresponding protein, eIF2D, has been shown to possess translation initiation factor activity |
| APC4 |
YDR118W |
Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to the nucleus increases upon DNA replication stress |
| VBA4 |
YDR119W |
Protein of unknown function; proposed role as a basic amino acid permease based on phylogeny; GFP-fusion protein localizes to vacuolar membrane; physical interaction with Atg27p suggests a possible role in autophagy; non-essential gene |
| COX26 |
YDR119W-A |
Uncharacterized protein YDR119W-A; Stabilizes or regulates formation of respiratory chain supercomplexes composed of Complex III (ubiquinol-cytochrome c reductase) and Complex IV (cytochrome c oxidase) |
| TRM1 |
YDR120C |
tRNA (guanine(26)-N(2))-dimethyltransferase, mitochondrial; tRNA methyltransferase; two forms of protein are made by alternative translation starts; localizes to both nucleus and mitochondrion to produce modified base N2,N2-dimethylguanosine in tRNAs in both compartments; nuclear Trm1p is evenly distributed around inner nuclear membrane in WT, but mislocalizes as puncta near ER-nucleus junctions in spindle pole body (SPB) mutants; both Trm1p inner nuclear membrane targeting and maintenance depend upon SPB |
| DPB4 |
YDR121W |
Subunit of DNA pol epsilon and of ISW2 chromatin accessibility complex; involved in both chromosomal DNA replication and inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; interacts with extranucleosomal DNA and acts as anchor point for ISW2 complex that retains its position on DNA during nucleosome mobilization |
| KIN1 |
YDR122W |
Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; KIN1 has a paralog, KIN2, that arose from the whole genome duplication |
| INO2 |
YDR123C |
Protein INO2; Transcription factor; component of the heteromeric Ino2p/Ino4p basic helix-loop-helix transcription activator that binds inositol/choline-responsive elements (ICREs), required for derepression of phospholipid biosynthetic genes in response to inositol depletion; involved in diauxic shift |
| YDR124W |
YDR124W |
Uncharacterized protein YDR124W; Putative protein of unknown function; non-essential gene; expression is strongly induced by alpha factor |
| ECM18 |
YDR125C |
Protein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication; Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily |
| SWF1 |
YDR126W |
Palmitoyltransferase that acts on transmembrane proteins; including the SNAREs Snc1p, Syn8p, Tlg1p and likely SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion |
| ARO1 |
YDR127W |
Pentafunctional arom protein; catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids; In the C-terminal section; belongs to the shikimate dehydrogenase family |
| MTC5 |
YDR128W |
Maintenance of telomere capping protein 5; Subunit of SEACAT, a subcomplex of the SEA complex; Mtc1p, along with Rtc1p and Sea4p, redundantly inhibit the TORC1 inhibitory role of the Iml1p/SEACIT (Iml1p-Npr2p-Npr3p) subcomplex, a GAP for GTPase Gtr1p (EGOC subunit) in response to amino acid limitation, thereby resulting in activation of TORC1 signaling; SEA is a coatomer-related complex that associates dynamicy with the vacuole; relative distribution to the vacuolar membrane decreases upon DNA replication stress |
| SAC6 |
YDR129C |
Plastin-1; Fimbrin, actin-bundling protein; cooperates with Scp1p in organization and maintenance of the actin cytoskeleton; phosphorylated by Cdc28p/Clb2p in metaphase on T103, to regulate conformation, and modulate actin filament binding affinity and actin cable dynamics; relocalizes from the plasma membrane to the cytoplasm upon DNA replication stress; human homologs PLS3 and LCP1 implicated in spinocerebellar ataxia type 2 (SCA2) can each complement yeast null mutant |
| FIN1 |
YDR130C |
Filament protein FIN1; Spindle pole body-related intermediate filament protein; forms cell cycle-specific filaments between spindle pole bodies in dividing cells; localizes to poles and microtubules of spindle during anaphase and contributes to spindle stability; involved in Glc7p localization and regulation; relative distribution to the nucleus increases upon DNA replication stress |
| YDR131C |
YDR131C |
Uncharacterized protein; F-box protein subunit of SCF ubiquitin ligase complex; substrate-specific adaptor subunit that recruits substrates to a core ubiquitination complex |
| MRX16 |
YDR132C |
Uncharacterized protein YDR132C; Protein of unknown function; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress; YDR132C has a paralog, YLR108C, that arose from the whole genome duplication |
| YCF1 |
YDR135C |
Metal resistance protein YCF1; Vacuolar glutathione S-conjugate transporter; ABC-C transporter of the ATP-binding cassette family; required for vacuole fusion; forms stable complexes with vacuole fusion machinery; regulates Vam7p recruitment to vacuoles; role in detoxifying metals (Cd, Hg, As); transports GSSG that is not immediately reduced in cytosol to vacuole; transports unconjugated bilirubin, selenodigluthatione, oxidized glutathione; similar to human cystic fibrosis protein CFTR |
| RGP1 |
YDR137W |
Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p); this complex catalyzes nucleotide exchange on Ypt6p; Belongs to the RGP1 family |
| HPR1 |
YDR138W |
Subunit of THO/TREX complexes; this complex couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p |
| RUB1 |
YDR139C |
NEDD8-like protein RUB1; Ubiquitin-like protein with similarity to mammalian NEDD8; conjugation (neddylation) substrates include the cullins Cdc53p, Rtt101p, and Cul3p; activated by Ula1p and Uba3p (E1 enzyme pair); conjugation mediated by Ubc12p (E2 enzyme) |
| MTQ2 |
YDR140W |
eRF1 methyltransferase catalytic subunit MTQ2; S-adenosylmethionine-dependent methyltransferase; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC; member of the seven beta-strand family |
| DOP1 |
YDR141C |
Protein dopey; Golgi-localized, leucine-zipper domain containing protein; involved in endosome to Golgi transport, organization of the ER, establishing cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies |
| PEX7 |
YDR142C |
Peroxisomal signal receptor for peroxisomal matrix proteins; recognizes the N-terminal nonapeptide signal (PTS2); WD repeat protein; defects in human homolog cause lethal rhizomelic chondrodysplasia punctata (RCDP) |
| SAN1 |
YDR143C |
Ubiquitin-protein ligase; involved in proteasome-dependent degradation of aberrant nuclear proteins; targets substrates with regions of exposed hydrophobicity containing 5 or more contiguous hydrophobic residues; contains intrinsicy disordered regions that contribute to substrate recognition; prefers a window of exposed hydrophobicity that causes a particular level of protein insolubility, suggesting that San1p evolved to target highly aggregation-prone proteins |
| MKC7 |
YDR144C |
Aspartic proteinase MKC7; GPI-anchored aspartyl protease; member of the yapsin family of proteases involved in cell w growth and maintenance; shares functions with Yap3p and Kex2p; MKC7 has a paralog, YPS1, that arose from the whole genome duplication |
| TAF12 |
YDR145W |
Subunit (61/68 kDa) of TFIID and SAGA complexes; involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A; overexpression of the human ortholog, TAF12, an oncogene involved in the formation of choroid plexus carcinomas, results in dosage chromosomal instability (dCIN) in a human cell line similar to the dCIN observed in yeast overexpressors |
| SWI5 |
YDR146C |
Transcriptional factor SWI5; Transcription factor that recruits Mediator and Swi/Snf complexes; activates transcription of genes expressed at the M/G1 phase boundary and in G1 phase; required for expression of the HO gene controlling mating type switching; localization to nucleus occurs during G1 and appears to be regulated by phosphorylation by Cdc28p kinase; SWI5 has a paralog, ACE2, that arose from the whole genome duplication |
| EKI1 |
YDR147W |
Ethanolamine kinase; primarily responsible for phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; exhibits some choline kinase activity, thus contributing to phosphatidylcholine synthesis via the CDP-choline pathway; EKI1 has a paralog, CKI1, that arose from the whole genome duplication |
| KGD2 |
YDR148C |
Dihydrolipoyl transsuccinylase; component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes the oxidative decarboxylation of alpha-ketoglutarate to succinyl-CoA in the TCA cycle; phosphorylated |
| NUM1 |
YDR150W |
Protein required for nuclear migration; component of the mitochondria-ER-cortex-ancor (MECA); required for the association of mitochondria with the cell cortex and for accurate distribution of mitochondrial network; interacts with Mdm36p to link the ER and mitochondria at the cortex; localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex |
| CTH1 |
YDR151C |
mRNA decay factor CTH1; Member of the CCCH zinc finger family; similar to mammalian Tis11 protein, which activates transcription and also has a role in mRNA degradation; may function with Tis11p in iron homeostasis; CTH1 has a paralog, TIS11, that arose from the whole genome duplication |
| GIR2 |
YDR152W |
Protein GIR2; Highly-acidic RWD domain-containing cytoplasmic protein; forms a highly conserved complex with Rbg2p that is responsible for efficient cell growth under amino acid starvation and binds translational activator Gcn1p in dose-dependent manner according to stress level; associates with translating ribosomes; intrinsicy unstructured protein whose stability is enhanced upon binding Rbg2p; Belongs to the RWDD1/GIR2 family |
| ENT5 |
YDR153C |
Epsin-5; Protein containing an N-terminal epsin-like domain; involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p, clathrin adaptor complex AP-1, and clathrin |
| CPR1 |
YDR155C |
Cytoplasmic peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds the drug cyclosporin A; N-terminy propionylated in vivo; protein abundance increases in response to DNA replication stress; Belongs to the cyclophilin-type PPIase family. PPIase A subfamily |
| RPA14 |
YDR156W |
Dna-directed rna polymerase i subunit rpa14; RNA polymerase I subunit A14 |
| YDR157W |
YDR157W |
Uncharacterized protein YDR157W; Putative protein of unknown function; conserved across S. cerevisiae strains |
| HOM2 |
YDR158W |
Aspartic beta semi-aldehyde dehydrogenase; catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis; Belongs to the aspartate-semialdehyde dehydrogenase family |
| SAC3 |
YDR159W |
Nuclear mRNA export protein SAC3; mRNA export factor; required for biogenesis of the sm ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) |
| SSY1 |
YDR160W |
SPS-sensor component SSY1; Component of the SPS plasma membrane amino acid sensor system; senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes; other members are Ssy1p, Ptr3p, and Ssy5p |
| ACL4 |
YDR161W |
Specific assembly chaperone for ribosomal protein Rpl4p; binds to an evolutionarily conserved surface extension of nascent Rpl4p and chaperones Rpl4p until its assembly into the pre-ribosome; transcriptiony co-regulated with rRNA and ribosome biosynthesis genes; Belongs to the ACL4 family |
| NBP2 |
YDR162C |
NAP1-binding protein 2; Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell w integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) |
| CWC15 |
YDR163W |
Pre-mRNA-splicing factor CWC15; Non-essential protein involved in pre-mRNA splicing; component of a complex containing Cef1p; has similarity to S. pombe Cwf15p; Belongs to the CWC15 family |
| SEC1 |
YDR164C |
Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function; interacts directly with essential exocyst subunit Sec6p |
| TRM82 |
YDR165W |
Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p; relocalizes to the cytosol in response to hypoxia; mutation in human ortholog WDR4 causes microcephalic primordial dwarfism |
| SEC5 |
YDR166C |
Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip; Belongs to the SEC5 family |
| TAF10 |
YDR167W |
Transcription initiation factor tfiid subunit 10; Subunit (145 kDa) of TFIID and SAGA complexes; involved in RNA polymerase II transcription initiation and in chromatin modification |
| CDC37 |
YDR168W |
Essential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycle; stabilizes protein kinase nascent chains and participates along with Hsp90p in their folding; Belongs to the CDC37 family |
| STB3 |
YDR169C |
Protein STB3; Ribosomal RNA processing element (RRPE)-binding protein; involved in the glucose-induced transition from quiescence to growth; restricted to nucleus in quiescent cells, released into cytoplasm after glucose repletion; binds Sin3p; relative distribution to the nucleus increases upon DNA replication stress |
| YDR169C-A |
YDR169C-A |
Uncharacterized protein YDR169C-A; Putative protein of unknown function; identified by fungal homology and RT-PCR |
| SEC7 |
YDR170C |
Protein transport protein SEC7; Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors; involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles |
| YNCD0016C |
YNCD0016C |
Unknown |
| HSP42 |
YDR171W |
Sm heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress unfolded protein aggregation; involved in cytoskeleton reorganization after heat shock; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress |
| SUP35 |
YDR172W |
Eukaryotic peptide chain release factor GTP-binding subunit; Translation termination factor eRF3; has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that modifies cellular fitness, alters translational fidelity by affecting reading frame selection, and results in a nonsense suppressor phenotype; many stress-response genes are repressed in the presence of [PSI(+)] |
| ARG82 |
YDR173C |
Inositol polyphosphate multikinase (IPMK); sequentiy phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes |
| HMO1 |
YDR174W |
Transcriptional regulator hmo1; Chromatin associated high mobility group (HMG) family member; involved in compacting, bending, bridging and looping DNA; rDNA-binding component that regulates transcription from RNA polymerase I promoters; regulates start site selection of ribosomal protein genes via RNA polymerase II promoters; role in genome maintenance; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase; relocalizes to the cytosol in response to hypoxia |
| RSM24 |
YDR175C |
Mitochondrial 37s ribosomal protein rsm24; Mitochondrial ribosomal protein of the sm subunit |
| NGG1 |
YDR176W |
Subunit of chromatin modifying histone acetyltransferase complexes; member of the ADA complex, the SAGA complex, and the SLIK complex; transcriptional regulator involved in glucose repression of Gal4p-regulated genes |
| UBC1 |
YDR177W |
Ubiquitin-conjugating enzyme; key E2 partner with Ubc4p for the anaphase-promoting complex (APC); mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress key E2 partner with Ubc4p for the anaphase-promoting complex (APC) |
| SDH4 |
YDR178W |
Succinate dehydrogenase [ubiquinone] cytochrome b sm subunit, mitochondrial; Membrane anchor subunit of succinate dehydrogenase (SDH); involved in coupling the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; has similarity to human SDH subunit D (SDHD), which is implicated in paraganglioma |
| CSN9 |
YDR179C |
Subunit of the Cop9 signalosome; Cop9 signalosome is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling |
| NVJ3 |
YDR179W-A |
Uncharacterized protein YDR179W-A; Protein with a potential role in tethering ER and vacuoles; localizes to nucleus-vacuole junctions in an Mdm1p-dependent manner; contains a lipid-binding PXA domain |
| SCC2 |
YDR180W |
Sister chromatid cohesion protein 2; Subunit of cohesin loading factor (Scc2p-Scc4p); a complex required for loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; promotes gene expression program that supports translational fidelity; evolutionarily-conserved adherin; relocalizes to cytosol in response to hypoxia; human disorder Cornelia de Lange syndrome is caused by mutations in NIPBL, the human ortholog of SCC2 |
| SAS4 |
YDR181C |
Something about silencing protein 4; Subunit of the SAS complex (Sas2p, Sas4p, Sas5p); acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p |
| CDC1 |
YDR182W |
Cell division control protein 1; Putative mannose-ethanolamine phosphate phosphodiesterase; involved in GPI-anchor remodeling prior to the attachment of cell w proteins to beta 1,3-glucan, removing ethanolamine phosphate from the first mannose of GPI anchors; mutants display elevated Ca2+-dependent signaling resulting in secondary actin polarization and Golgi inheritance defects; enzyme is Mn2+-dependent; mutants have cell division cycle defect and fragile cell ws; Belongs to the metophosphoesterase superfamily. MPPE1 family |
| YDR182W-A |
YDR182W-A |
Uncharacterized protein YDR182W-A; Putative protein of unknown function; identified by fungal homology and RT-PCR |
| PLP1 |
YDR183W |
Phosducin-like protein 1; Protein that interacts with CCT (chaperonin containing TCP-1) complex; has a role in actin and tubulin folding; has weak similarity to phosducins, which are G-protein regulators; Belongs to the phosducin family |
| ATC1 |
YDR184C |
Nuclear protein; possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; relative distribution to the nucleus decreases upon DNA replication stress |
| UPS3 |
YDR185C |
Mitochondrial protein of unknown function; similar to Ups1p and Ups2p which are involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null is viable but interacts syntheticy with ups1 and ups2 mutations; UPS3 has a paralog, UPS2, that arose from the whole genome duplication |
| SND1 |
YDR186C |
Protein involved in SRP-independent targeting of substrates to the ER; component of an alternative ER targeting pathway that has partial functional redundancy with the GET pathway; preference for substrates with downstream transmembrane domains; interacts with Env10p/Snd2p and Pho88p/Snd3p; can compensate for loss of SRP; may interact with ribosomes, based on co-purification experiments; GFP-fusion protein localizes to the cytoplasm |
| CCT6 |
YDR188W |
T-complex protein 1 subunit zeta; Subunit of the cytosolic chaperonin Cct ring complex; related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif |
| SLY1 |
YDR189W |
Hydrophilic protein involved in ER/Golgi vesicle trafficking; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex |
| RVB1 |
YDR190C |
RuvB-like protein 1; ATP-dependent DNA helicase, also known as pontin; member of the AAA+ and RuvB-like protein families; similar to Rvb2p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly |
| HST4 |
YDR191W |
NAD-dependent histone deacetylase HST4; NAD(+)-dependent protein deacetylase; deacetylation targets are primarily mitochondrial proteins; involved along with Hst3p in silencing at telomeres, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism; accumulates in mitochondria in response to biotin starvation and may link biotin metabolism with energy homeostasis; member of the Sir2 family and may be the functional equivalent of human SIRT3 |
| NUP42 |
YDR192C |
FG-nucleoporin component of central core of the nuclear pore complex; also part of the nuclear pore complex (NPC) cytoplasmic filaments; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; interacts with Gle1p |
| MSS116 |
YDR194C |
ATP-dependent RNA helicase MSS116, mitochondrial; Mitochondrial transcription elongation factor; DEAD-box protein; required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing; promotes RNA folding by stabilizing an early assembly intermediate |
| YDR194W-A |
YDR194W-A |
Uncharacterized protein YDR194W-A; Putative protein of unknown function; identified by fungal homology and RT-PCR |
| REF2 |
YDR195W |
RNA end formation protein 2; RNA-binding protein; involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes; putative regulatory subunit of type 1 protein phosphatase Glc7p, required for actomyosin ring formation, and for timely dephosphorylation and release of Bnr1p from the division site; relocalizes to the cytosol in response to hypoxia |
| CAB5 |
YDR196C |
Dephospho-CoA kinase CAB5; Subunit of the CoA-Synthesizing Protein Complex (CoA-SPC); subunits of this complex are: Cab2p, Cab3p, Cab4p, Cab5p, Sis2p and Vhs3p; probable dephospho-CoA kinase (DPCK) that catalyzes the last step in coenzyme A biosynthesis; null mutant lethality is complemented by human homolog DCAKD and by E. coli coaE (encoding DPCK); detected in purified mitochondria in high-throughput studies; also localized to lipid droplets |
| CBS2 |
YDR197W |
Cytochrome b translational activator protein cbs2, mitochondrial; Mitochondrial translational activator of the COB mRNA; interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader |
| RKM2 |
YDR198C |
Ribosomal lysine n-methyltransferase 2; Ribosomal protein lysine methyltransferase; responsible for trimethylation of the lysine residue at position 3 of Rpl12Ap and Rpl12Bp |
| VPS64 |
YDR200C |
Vacuolar protein sorting-associated protein 64; Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance; VPS64 has a paralog, FAR10, that arose from the whole genome duplication |
| SPC19 |
YDR201W |
Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body |
| RAV2 |
YDR202C |
Regulator of V-ATPase in vacuolar membrane protein 2; Subunit of RAVE complex (Rav1p, Rav2p, Skp1p); the RAVE complex associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme |
| COQ4 |
YDR204W |
Protein with a role in ubiquinone (Coenzyme Q) biosynthesis; possibly functioning in stabilization of Coq7p; located on matrix face of mitochondrial inner membrane; component of a mitochondrial ubiquinone-synthesizing complex; human homolog COQ4 can complement yeast coq4 null mutant |
| MSC2 |
YDR205W |
Solute carrier family 30 (zinc transporter), member 5/7; Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids |
| EBS1 |
YDR206W |
Protein involved in translation inhibition and nonsense-mediated decay; interacts with cap binding protein Cdc33p and with Nam7p; localizes to P-bodies upon glucose starvation; mRNA abundance regulated by mRNA decay factors; EBS1 has a paralog, EST1, that arose from the whole genome duplication |
| UME6 |
YDR207C |
Transcriptional regulatory protein UME6; Rpd3L histone deacetylase complex subunit; key transcriptional regulator of early meiotic genes; involved in chromatin remodeling and transcriptional repression via DNA looping; binds URS1 upstream regulatory sequence, represses transcription by recruiting conserved histone deacetylase Rpd3p (through co-repressor Sin3p) and chromatin-remodeling factor Isw2p; couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms compl |
| MSS4 |
YDR208W |
Phosphatidylinositol-4-phosphate 5-kinase; involved in actin cytoskeleton organization and cell morphogenesis; multicopy suppressor of stt4 mutation |
| YDR209C |
YDR209C |
Uncharacterized protein YDR209C; Putative protein of unknown function; conserved among S. cerevisiae strains; YDR209C is not an essential gene; partiy overlaps uncharacterized gene YDR210W |
| YDR210W |
YDR210W |
Cysteine-rich and transmembrane domain-containing protein YDR210W; Predicted tail-anchored plasma membrane protein; contains a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; Belongs to the CYSTM1 family |
| YNCD0017W |
YNCD0017W |
Unknown |
| GCD6 |
YDR211W |
Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress; Belongs to the eIF-2B gamma/epsilon subunits family |
| TCP1 |
YDR212W |
Alpha subunit of chaperonin-containing T-complex; complex mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein |
| UPC2 |
YDR213W |
Sterol uptake control protein 2; Sterol regulatory element binding protein; induces sterol biosynthetic genes, upon sterol depletion; acts as a sterol sensor, binding ergosterol in sterol rich conditions; relocates from intracellular membranes to perinuclear foci upon sterol depletion; redundant activator of filamentation with ECM22, up-regulating the expression of filamentous growth genes; contains a Zn[2]-Cys[6] binuclear cluster; UPC2 has a paralog, ECM22, that arose from the whole genome duplication |
| AHA1 |
YDR214W |
Hsp90 co-chaperone AHA1; Co-chaperone that binds Hsp82p and activates its ATPase activity; plays a role in determining prion variants; similar to Hch1p; expression is regulated by stresses such as heat shock; protein abundance increases in response to DNA replication stress; Belongs to the AHA1 family |
| YDR215C |
YDR215C |
Uncharacterized protein YDR215C; Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein |
| ADR1 |
YDR216W |
Regulatory protein ADR1; Carbon source-responsive zinc-finger transcription factor; required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization |
| RAD9 |
YDR217C |
DNA damage-dependent checkpoint protein; required for cell-cycle arrest in G1/S, intra-S, and G2/M, plays a role in postreplication repair (PRR) pathway; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; multiple cyclin dependent kinase consensus sites and the C-terminal BRCT domain contribute to DNA damage checkpoint activation; Rad9p Chk1 Activating Domain (CAD) is phosphorylated at multiple sites by Cdc28p/Clb2p |
| SPR28 |
YDR218C |
Sporulation-regulated protein 28; Sporulation-specific homolog of the CDC3/10/11/12 family of genes; meiotic septin expressed at high levels during meiotic divisions and ascospore formation; the yeast CDC3/10/11/12 family is a family of bud neck microfilament genes; Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin- like GTPase superfamily. Septin GTPase family |
| MFB1 |
YDR219C |
Mitochondria-associated F-box protein; involved in maintenance of normal mitochondrial morphology; interacts with Skp1p through the F-box motif; preferentiy localizes to the mother cell during budding |
| GTB1 |
YDR221W |
Glucosidase II beta subunit, forms a complex with alpha subunit Rot2p; involved in removal of two glucose residues from N-linked glycans during glycoprotein biogenesis in the ER; relocalizes from ER to cytoplasm upon DNA replication stress |
| YDR222W |
YDR222W |
SVF1-like protein YDR222W; Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YDR222W has a paralog, YLR225C, that arose from the whole genome duplication; Belongs to the SVF1 family |
| CRF1 |
YDR223W |
Transcription factor CRF1; Transcriptional corepressor; involved in repression of ribosomal protein (RP) gene transcription via the TOR signaling pathway which promotes accumulation of Crf1p in the nucleus; role in repression of RP genes varies by strain; CRF1 has a paralog, IFH1, that arose from the whole genome duplication |
| HTB1 |
YDR224C |
Histone H2B; core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates reassembly after DNA replication, transcriptional activation, meiotic DSB formation and H3 methylation |
| HTA1 |
YDR225W |
Histone H2A; core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p; N-terminy propionylated in vivo |
| ADK1 |
YDR226W |
Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress; mutations affecting Adk1p catalytic activity deregulate expression of phosphate utilization genes PHO5 and PHO84; human homolog AK1 can complement yeast adk1 mutant |
| SIR4 |
YDR227W |
Regulatory protein SIR4; SIR protein involved in assembly of silent chromatin domains; silent information regulator (SIR) along with SIR2 and SIR3; involved in assembly of silent chromatin domains at telomeres and the silent mating-type loci; some eles of SIR4 prolong lifespan; required for telomere hypercluster formation in quiescent yeast cells |
| PCF11 |
YDR228C |
Protein PCF11; mRNA 3' end processing factor; essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping; relocalizes to the cytosol in response to hypoxia |
| IVY1 |
YDR229W |
Protein IVY1; Phospholipid-binding protein that interacts with both Ypt7p and Vps33p; may partiy counteract the action of Vps33p and vice versa, localizes to the rim of the vacuole as cells approach stationary phase |
| COX20 |
YDR231C |
Mitochondrial inner membrane protein; required for proteolytic processing of Cox2p and its assembly into cytochrome c oxidase; Belongs to the COX20 family |
| HEM1 |
YDR232W |
5-aminolevulinate synthase, mitochondrial; 5-aminolevulinate synthase; catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p; has a pyridoxal phosphate cofactor whose insertion is mediated by Mcx1p; Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family |
| RTN1 |
YDR233C |
Reticulon-like protein 1; Reticulon protein; involved in nuclear pore assembly and maintenance of tubular ER morphology; promotes membrane curvature; regulates the ER asymmetry-induced inheritance block during ER stress; role in ER-derived peroxisomal biogenesis; increases tubular ER when overexpressed; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; member of the RTNLA subfamily |
| LYS4 |
YDR234W |
Homoaconitase, mitochondrial; Homoaconitase; catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway |
| PRP42 |
YDR235W |
U1 sm nuclear ribonucleoprotein component PRP42; U1 snRNP protein involved in splicing; required for U1 snRNP biogenesis; contains multiple tetriatricopeptide repeats |
| FMN1 |
YDR236C |
Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane; human RFK functiony complements the lethality of the null mutation |
| MRPL7 |
YDR237W |
Mitochondrial 54s ribosomal protein yml7/yml5; Mitochondrial ribosomal protein of the large subunit; MRPL7 produces both YmL5 and YmL7, which are two different modified forms of the same protein |
| SEC26 |
YDR238C |
Coatomer subunit beta; Essential beta-coat protein of the COPI coatomer; involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) |
| YDR239C |
YDR239C |
Uncharacterized protein YDR239C; Protein of unknown function; may interact with ribosomes, based on co-purification experiments |
| SNU56 |
YDR240C |
56 kDa U1 sm nuclear ribonucleoprotein component; Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex |
| SUP2 |
YNCD0018W |
Unknown |
| AMD2 |
YDR242W |
Probable amidase; Putative amidase |
| PRP28 |
YDR243C |
Pre-mRNA-splicing ATP-dependent RNA helicase PRP28; RNA binding protein; involved in RNA isomerization at the 5' splice site and for exchange of U6 for U1 snRNA at the 5' splice site; similar to the RNA helicases of the DEAD-box family |
| PEX5 |
YDR244W |
Peroxisomal membrane signal receptor for peroxisomal matrix proteins; receptor for the C-terminal tripeptide signal sequence (PTS1) of peroxisomal matrix proteins; required for peroxisomal matrix protein import; also proposed to have PTS1-receptor independent functions |
| MNN10 |
YDR245W |
Probable alpha-1,6-mannosyltransferase MNN10; Subunit of a Golgi mannosyltransferase complex; complex mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family; other members of the complex are Anp1p, Mnn9p, Mnn11p, and Hoc1p; Belongs to the glycosyltransferase 34 family |
| TRS23 |
YDR246W |
Core component of transport protein particle (TRAPP) complexes I-III; TRAPP complexes are related multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating ER-Golgi traffic (TRAPPI), intra-Golgi traffic (TRAPPII), endosome-Golgi traffic (TRAPPII and III) and autophagy (TRAPPIII); human homolog is TRAPPC4 |
| YDR246W-A |
YDR246W-A |
Uncharacterized protein YDR246W-A; Putative protein of unknown function; identified by fungal homology and RT-PCR |
| VHS1 |
YDR247W |
Serine/threonine-protein kinase VHS1; Cytoplasmic serine/threonine protein kinase; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; VHS1 has a paralog, SKS1, that arose from the whole genome duplication |
| YDR248C |
YDR248C |
Putative gluconokinase; sequence similarity to bacterial and human gluconokinase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; upregulated by deletion of the RNAP-II associated factor, PAF1 |
| YDR249C |
YDR249C |
Uncharacterized protein YDR249C; Putative protein of unknown function |
| PAM1 |
YDR251W |
Essential protein of unknown function; exhibits variable expression during colony morphogenesis; overexpression permits survival without protein phosphatase 2A, inhibits growth, and induces a filamentous phenotype; PAM1 has a paralog, SVL3, that arose from the whole genome duplication |
| BTT1 |
YDR252W |
Heterotrimeric nascent polypeptide-associated complex beta3 subunit; complex binds ribosomes via its beta-subunits in close proximity to nascent polypeptides; interacts with Caf130p of the CCR4-NOT complex; similar to human BTF3; BTT1 has a paralog, EGD1, that arose from the whole genome duplication |
| MET32 |
YDR253C |
Transcriptional regulator MET32; Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; targets strong transcriptional activator Met4p to promoters of sulfur metabolic genes; feedforward loop exists in the regulation of genes controlled by Met4p and Met32p; lack of such a loop for MET31 may account for the differential actions of Met32p and Met31p; MET32 has a paralog, MET31, that arose from the whole genome duplication |
| CHL4 |
YDR254W |
Central kinetochore subunit CHL4; Outer kinetochore protein required for chromosome stability; involved in new kinetochore assembly and sister chromatid cohesion; forms a stable complex with Iml3p; peripheral component of the Ctf19 kinetochore complex that interacts with Ctf19p, Ctf3p, and Mif2p; required for the spindle assembly checkpoint; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-N and fission yeast mis15 |
| RMD5 |
YDR255C |
E3 ubiquitin-protein transferase RMND5; Sporulation protein RMD5; Component of GID Complex that confers ubiquitin ligase (U3) activity; necessary for polyubiquitination and degradation of the gluconeogenic enzyme fructose-1,6-bisphosphatase; forms dimer with Fyv10p that is then recruited to GID Complex by Gid8p; also required for sporulation; conserved protein that has a degenerate RING finger domain |
| CTA1 |
YDR256C |
Catalase A; breaks down hydrogen peroxide in the peroxisomal matrix formed by acyl-CoA oxidase (Pox1p) during fatty acid beta-oxidation; Belongs to the catalase family |
| RKM4 |
YDR257C |
Ribosomal lysine methyltransferase; specific for monomethylation of Rpl42ap and Rpl42bp (lysine 55); nuclear SET-domain containing protein |
| HSP78 |
YDR258C |
Atp-dependent clp protease atp-binding subunit clpb; Heat shock protein 78, mitochondrial; Oligomeric mitochondrial matrix chaperone; cooperates with Ssc1p in mitochondrial thermotolerance after heat shock; able to prevent the aggregation of misfolded proteins as well as resolubilize protein aggregates |
| YAP6 |
YDR259C |
Basic leucine zipper (bZIP) transcription factor; physicy interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; overexpression increases sodium and lithium tolerance; computational analysis suggests a role in regulation of expression of genes involved in carbohydrate metabolism; YAP6 has a paralog, CIN5, that arose from the whole genome duplication |
| SWM1 |
YDR260C |
Subunit of the anaphase-promoting complex (APC); APC is an E3 ubiquitin ligase that regulates the metaphase-anaphase transition and exit from mitosis; required for activation of the daughter-specific gene expression and spore w maturation; Belongs to the APC13 family |
| EXG2 |
YDR261C |
Glucan 1,3-beta-glucosidase 2; Exo-1,3-beta-glucanase; involved in cell w beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor |
| YNCD0019C |
YNCD0019C |
Unknown |
| YDR170W-A |
YDR170W-A |
Retrotransposon TYA Gag gene; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag; YDR170W-A is part of a mutant retrotransposon; distribution in the cytoplasm becomes irregular rather than punctate upon DNA replication stress |
| YDR262W |
YDR262W |
FAS1 domain-containing protein YDR262W; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole and is induced in response to the DNA-damaging agent MMS; gene expression increases in response to Zymoliase treatment |
| DIN7 |
YDR263C |
DNA damage-inducible protein DIN7; Mitochondrial nuclease functioning in DNA repair and replication; modulates the stability of the mitochondrial genome, induced by exposure to mutagens, also induced during meiosis at a time nearly coincident with commitment to recombination; DIN7 has a paralog, EXO1, that arose from the whole genome duplication; Belongs to the XPG/RAD2 endonuclease family |
| AKR1 |
YDR264C |
Palmitoyltransferase AKR1; Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication; any of several human homologs encoding DHHC-type zinc fingers (ZDHHC) can complement temperature sensitivity of yeast akr1 null mutant; Belongs to the DHHC palmitoyltransferase family. AKR/ZDHHC17 subfamily |
| PEX10 |
YDR265W |
Peroxisome biogenesis factor 10; Peroxisomal membrane E3 ubiquitin ligase; required for for Ubc4p-dependent Pex5p ubiquitination and peroxisomal matrix protein import; contains zinc-binding RING domain; mutations in human homolog cause various peroxisomal disorders |
| HEL2 |
YDR266C |
RING finger ubiquitin ligase (E3); involved in ubiquitination and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; computational analysis suggests a role as a transcription factor |
| CIA1 |
YDR267C |
Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at late step of Fe-S cluster assembly; forms CIA targeting complex with Cia2p and Met18p that directs Fe-S cluster incorporation into subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, telomere maintenance; contains WD40 repeats; human homolog CIAO1 can complement yeast cia1 null mutant |
| MSW1 |
YDR268W |
Tryptophan--tRNA ligase, mitochondrial; Mitochondrial tryptophanyl-tRNA synthetase; Belongs to the class-I aminoacyl-tRNA synthetase family |
| CCC2 |
YDR270W |
Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; similar to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism; human homologs ATP7A and ATP7B both complement yeast null mutant |
| GLO2 |
YDR272W |
Hydroxyacylglutathione hydrolase, cytoplasmic isozyme; Cytoplasmic glyoxalase II; catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate; GLO2 has a paralog, GLO4, that arose from the whole genome duplication |
| DON1 |
YDR273W |
Donuts protein 1; Meiosis-specific component of the spindle pole body; subunit of the leading edge protein (LEP) complex (Ssp1-Ady3-Don1-Irc10) that forms a ring-like structure at the leading edge of the prospore membrane (PSM) during meiosis II; required for PSM growth and closure; DON1 has a paralog, CUE5, that arose from the whole genome |
| YDR274C |
YDR274C |
Uncharacterized protein YDR274C; Putative protein of unknown function; conserved among S. cerevisiae strains; YDR274C is not an essential gene |
| BSC2 |
YDR275W |
Bypass of stop codon protein 2; Protein of unknown function; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; null mutant displays increased translation rate and increased readthrough of premature stop codons; BSC2 has a paralog, IRC23, that arose from the whole genome duplication |
| PMP3 |
YDR276C |
Sm plasma membrane protein; confers resistance to amphotericin B and is a potential target of this common antifungal drug; related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature; not essential for viability; deletion causes hyperpolarization of the plasma membrane potential |
| MTH1 |
YDR277C |
Protein MTH1; Negative regulator of the glucose-sensing signal transduction pathway; required for repression of transcription by Rgt1p; interacts with Rgt1p and the Snf3p and Rgt2p glucose sensors; phosphorylated by Yck1p, triggering Mth1p degradation; MTH1 has a paralog, STD1, that arose from the whole genome duplication |
| YDR278C |
YDR278C |
Uncharacterized protein YDR278C; Putative protein of unknown function; conserved among S. cerevisiae strains; YDR278C is not an essential gene |
| YNCD0021C |
YNCD0021C |
Unknown |
| RNH202 |
YDR279W |
Ribonuclease H2 subunit; required for RNase H2 activity; role in ribonucleotide excision repair; related to human AGS2 that causes Aicardi-Goutieres syndrome |
| RRP45 |
YDR280W |
Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress |
| PHM6 |
YDR281C |
Phosphate metabolism protein 6; Protein of unknown function; expression is regulated by phosphate levels |
| MRX10 |
YDR282C |
Required for meiotic nuclear division protein 1; MIOREX complex component 10; Mitochondrial inner membrane protein of unknown function; associates with mitochondrial ribosome; localizes to the inner membrane with the C terminus facing the intermembrane space; ortholog of human RMND1, mutation in which is implicated in infantile encephaloneuromyopathy and defective mitochondrial translation |
| GCN2 |
YDR283C |
eIF-2-alpha kinase GCN2; Protein kinase; phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control |
| DPP1 |
YDR284C |
Diacylglycerol pyrophosphate (DGPP) phosphatase; zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism |
| ZIP1 |
YDR285W |
Transverse filament protein of the synaptonemal complex; required for normal levels of meiotic recombination and pairing between homologous chromosome during meiosis; required for meiotic recombination between non-elc sites; potential Cdc28p substrate |
| MGP12 |
YDR286C |
Glutaredoxin-like protein YDR286C; Putative protein of unknown function; predicted to have thiol-disulfide oxidoreductase active site; Belongs to the glutaredoxin family. YDR286C subfamily |
| INM2 |
YDR287W |
Inositol monophosphatase; involved in biosynthesis of inositol; enzymatic activity requires magnesium ions and is inhibited by lithium and sodium ions; inm1 inm2 double mutant lacks inositol auxotrophy |
| NSE3 |
YDR288W |
Non-structural maintenance of chromosome element 3; Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress |
| RTT103 |
YDR289C |
Regulator of Ty1 transposition protein 103; Protein involved in transcription termination by RNA polymerase II; interacts with exonuclease Rat1p and Rai1p; has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition; Belongs to the UPF0400 (RTT103) family |
| HRQ1 |
YDR291W |
ATP-dependent helicase HRQ1; 3'-5' DNA helicase that has DNA strand annealing activity; helicase activity is stimulated by fork structure and 3'-tail length of substrates; acts with Rad4p in nucleotide-excision repair; belongs to the widely conserved RecQ family of proteins which are involved in maintaining genomic integrity; similar to the human RecQ4p implicated in Rothmund-Thomson syndrome (RTS) |
| SRP101 |
YDR292C |
Signal recognition particle (SRP) receptor alpha subunit; contain GTPase domains; involved in SRP-dependent protein targeting; interacts with the beta subunit, Srp102p |
| SSD1 |
YDR293C |
Protein SSD1; Translational repressor with a role in polar growth and w integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function; Belongs to the RNR ribonuclease family |
| DPL1 |
YDR294C |
Sphinganine-1-phosphate aldolase DPL1; Dihydrosphingosine phosphate lyase; regulates intracellular levels of sphingolipid long-chain base phosphates (LCBPs), degrades phosphorylated long chain bases, prefers C16 dihydrosphingosine-l-phosphate as a substrate |
| HDA2 |
YDR295C |
Subunit of the HDA1 histone deacetylase complex; possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex contains Hda1p homodimer and an Hda2p-Hda3p heterodimer; involved in telomere maintenance; relocalizes to the cytosol in response to hypoxia |
| MHR1 |
YDR296W |
Mitochondrial ribosomal protein of the large subunit; also involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress |
| SUR2 |
YDR297W |
Sphingolipid C4-hydroxylase SUR2; Sphinganine C4-hydroxylase; catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis; Belongs to the sterol desaturase family |
| ATP5 |
YDR298C |
Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated; Belongs to the ATPase delta chain family |
| BFR2 |
YDR299W |
Protein BFR2; Component of the SSU and 90S preribosomes; involved in pre-18S rRNA processing; binds to U3 snoRNA and Mpp10p; multicopy suppressor of sensitivity to Brefeldin A; expression is induced during lag phase and also by cold shock |
| PRO1 |
YDR300C |
Glutamate 5-kinase; Gamma-glutamyl kinase; catalyzes the first step in proline biosynthesis; required for nitrogen starvation-induced ribophagy but not for nonselective autophagy; PRO1 has a paralog, YHR033W, that arose from the whole genome duplication; Belongs to the glutamate 5-kinase family |
| CFT1 |
YDR301W |
Protein CFT1; RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF |
| GPI11 |
YDR302W |
Glycosylphosphatidylinositol anchor biosynthesis protein 11; ER membrane protein involved in a late step of GPI anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated glycosylphosphatidylinositol (GPI) intermediate; human PIG-Fp is a functional homolog |
| RSC3 |
YDR303C |
Chromatin structure-remodeling complex protein RSC3; Component of the RSC chromatin remodeling complex; essential gene required for maintenance of proper ploidy and regulation of ribosomal protein genes and the cell w/stress response; RSC3 has a paralog, RSC30, that arose from the whole genome duplication |
| CPR5 |
YDR304C |
Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the ER; catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; transcriptiony induced in response to unfolded proteins in the ER; CPR5 has a paralog, CPR2, that arose from the whole genome duplication |
| HNT2 |
YDR305C |
Bis(5'-adenosyl)-triphosphatase; Dinucleoside triphosphate hydrolase; has similarity to the tumor suppressor FHIT and belongs to the histidine triad (HIT) superfamily of nucleotide-binding proteins |
| PFU1 |
YDR306C |
F-box protein ydr306c; F-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain |
| YNCD0022C |
YNCD0022C |
Unknown |
| PMT7 |
YDR307W |
Probable dolichyl-phosphate-mannose--protein mannosyltransferase 7; Putative protein mannosyltransferase similar to Pmt1p; has a potential role in protein O-glycosylation; Belongs to the glycosyltransferase 39 family |
| SRB7 |
YDR308C |
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; target of the global repressor Tup1p |
| GIC2 |
YDR309C |
GTPase-interacting component 2; Redundant rho-like GTPase Cdc42p effector; involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain and with PI(4,5)P2 via a polybasic region; GIC2 has a paralog, GIC1, that arose from the whole genome duplication |
| SUM1 |
YDR310C |
Suppressor of mar1-1 protein; Transcriptional repressor that regulates middle-sporulation genes; required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint |
| TFB1 |
YDR311W |
Subunit of TFIIH and nucleotide excision repair factor 3 complexes; required for nucleotide excision repair, target for transcriptional activators; relocalizes to the cytosol in response to hypoxia |
| SSF2 |
YDR312W |
Ribosome biogenesis protein SSF2; Protein required for ribosomal large subunit maturation; functiony redundant with Ssf1p; member of the Brix family; SSF2 has a paralog, SSF1, that arose from the whole genome duplication |
| PIB1 |
YDR313C |
E3 ubiquitin-protein ligase PIB1; RING-type ubiquitin ligase of the endosomal and vacuolar membranes; binds phosphatidylinositol(3)-phosphate; contains a FYVE finger domain |
| RAD34 |
YDR314C |
Dna repair protein rad34; Protein involved in nucleotide excision repair (NER); homologous to RAD4 |
| IPK1 |
YDR315C |
Inositol 1,3,4,5,6-pentakisphosphate 2-kinase; nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable; human IPPK can complement ipk1 null mutant |
| OMS1 |
YDR316W |
Methyltransferase OMS1, mitochondrial; Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif and is predicted to be an RNA methyltransferase; multicopy suppressor of respiratory defects caused by OXA1 mutations |
| YNCD0023C |
YNCD0023C |
Unknown |
| HIM1 |
YDR317W |
Protein him1; Protein of unknown function involved in DNA repair |
| MCM21 |
YDR318W |
Central kinetochore subunit MCM21; Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; modified by sumoylation; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2 |
| YFT2 |
YDR319C |
FIT family protein YFT2; Protein required for normal ER membrane biosynthesis; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens |
| SWA2 |
YDR320C |
Auxilin-like clathrin uncoating factor SWA2; Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles |
| DAD4 |
YDR320C-A |
Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis |
| ASP1 |
YDR321W |
Cytosolic L-asparaginase, involved in asparagine catabolism; catalyzes hydrolysis of L-asparagine to aspartic acid and ammonia; important enzyme for the treatment of acute lymphoblastic leukemia; has low glutaminase activity and dependence; synthesized constitutively |
| MRPL35 |
YDR322W |
Mitochondrial 54s ribosomal protein yml35; Mitochondrial ribosomal protein of the large subunit; Belongs to the phosphatidylethanolamine-binding protein family. Mitochondrion-specific ribosomal protein mL38 subfamily |
| TIM11 |
YDR322C-A |
Subunit e of mitochondrial F1F0-ATPase; ATPase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; essential for the dimeric and oligomeric state of ATP synthase, which in turn determines the shape of inner membrane cristae |
| PEP7 |
YDR323C |
Adaptor protein involved in vesicle-mediated vacuolar protein sorting; multivalent adaptor protein; facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance |
| UTP4 |
YDR324C |
U3 sm nucleolar RNA-associated protein 4; Subunit of U3-containing 90S preribosome and SSU processome complexes; involved in production of 18S rRNA and assembly of sm ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript; Sm Subunit processome is also known as SSU processome |
| YCG1 |
YDR325W |
Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding by the complex; required for tRNA genes clustering at the nucleolus; required for replication slow zone breakage following Mec1p inactivation; transcription is cell cycle regulated, peaking in mitosis and declining in G1; protein is constitutively degraded by the proteasome; rate limiting for condensin recruitment to chromatin |
| YSP2 |
YDR326C |
Membrane-anchored lipid-binding protein YSP2; Sterol-binding protein; has a probable role in retrograde transport of sterols from the plasma membrane to the ER; contains two StART-like domains that bind sterols and a GRAM domain; co-localizes to puncta in the cortical ER with Sip3p; one of six StART-like domain-containing proteins in yeast that may be involved in sterol transfer between intracellular membranes; conserved across eukaryotes |
| SKP1 |
YDR328C |
S-phase kinase-associated protein 1; Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress |
| PEX3 |
YDR329C |
Peroxisomal biogenesis factor 3; Peroxisomal membrane protein (PMP); required for proper localization and stability of PMPs; anchors peroxisome retention factor Inp1p at the peroxisomal membrane; interacts with Pex19p |
| UBX5 |
YDR330W |
Ubx domain-containing protein 5; UBX domain-containing protein that interacts with Cdc48p; ubiquitin regulatory X is also known as UBX |
| GPI8 |
YDR331W |
GPI-anchor transamidase; ER membrane glycoprotein subunit of the GPI transamidase complex; adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog |
| IRC3 |
YDR332W |
Putative ATP-dependent helicase IRC3; Double-stranded DNA-dependent helicase of the DExH/D-box family; required for maintenance of the mitochondrial (mt) genome; null mutant accumulates double-stranded breaks in mt DNA; localizes to the mt matrix; Belongs to the helicase family. IRC3 subfamily |
| RQC1 |
YDR333C |
Component of the ribosome quality control complex (RQC); RQC (Rqc1p-Rkr1p-Tae2p-Cdc48p-Npl4p-Ufd1p) is a ribosome-bound complex required for the degradation of polypeptides arising from sted translation; required along with Rkr1p for recruitment of the Cdc48p-Npl4p-Ufd1p AAA ATPase complex to the RQC; Belongs to the TCF25 family |
| SWR1 |
YDR334W |
Helicase SWR1; Swi2/Snf2-related ATPase; structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; relocalizes to the cytosol in response to hypoxia; chronological aging factor that mediates lifespan extension by dietary restriction; Belongs to the SNF2/RAD54 helicase family. SWR1 subfamily |
| MSN5 |
YDR335W |
Protein MSN5; Karyopherin; involved in nuclear import and export of proteins, including import of replication protein A and export of Far1p and transcription factors Swi5p, Swi6p, Msn2p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm; exportin-5 homolog |
| MRX8 |
YDR336W |
MIOREX complex component 8; Protein that associates with mitochondrial ribosome; sumoylated under stress conditions in a genome wide study; YDR336W is not an essential gene |
| MRPS28 |
YDR337W |
Mitochondrial 37s ribosomal protein mrps28; Mitochondrial ribosomal protein of the sm subunit |
| YDR338C |
YDR338C |
Uncharacterized transporter YDR338C; Putative protein of unknown function; member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily |
| FCF1 |
YDR339C |
rRNA-processing protein FCF1; Putative PINc domain nuclease; required for early cleavages of 35S pre-rRNA and maturation of 18S rRNA; component of the SSU (sm subunit) processome involved in 40S ribosomal subunit biogenesis; copurifies with Faf1p |
| YNCD0024C |
YNCD0024C |
Unknown |
| YDR341C |
YDR341C |
Arginine--tRNA ligase, cytoplasmic; Arginyl-tRNA synthetase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDR341C has a paralog, MSR1, that arose from the whole genome duplication |
| HXT6 |
YDR343C |
High-affinity glucose transporter; member of the major facilitator superfamily, nearly identical to Hxt7p, expressed at high basal levels relative to other HXTs, repression of expression by high glucose requires SNF3; HXT6 has a paralog, HXT1, that arose from the whole genome duplication |
| YDR344C |
YDR344C |
Uncharacterized protein YDR344C; Putative protein of unknown function; conserved among S. cerevisiae strains |
| HXT3 |
YDR345C |
Mfs transporter, sp family, sugar:h+ symporter; Low affinity glucose transporter of the major facilitator superfamily; expression is induced in low or high glucose conditions; HXT3 has a paralog, HXT5, that arose from the whole genome duplication |
| SVF1 |
YDR346C |
Survival factor 1; Protein with a potential role in cell survival pathways; required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance; Belongs to the SVF1 family |
| MRP1 |
YDR347W |
37S ribosomal protein MRP1, mitochondrial; Mitochondrial ribosomal protein of the sm subunit; MRP1 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator, and with PET123, encoding a sm subunit mitochondrial ribosomal protein |
| PAL1 |
YDR348C |
Protein of unknown function thought to be involved in endocytosis; physicy interacts with Ede1p and is found at endocytic sites at cell periphery during early stages of endocytosis; green fluorescent protein (GFP)-fusion protein localizes to bud neck; potential Cdc28p substrate; similar to S. pombe Pal1 protein; relocalizes from bud neck to cytoplasm upon DNA replication stress; PAL1 has a paralog, YHR097C, that arose from the whole genome duplication |
| YPS7 |
YDR349C |
Aspartic proteinase yapsin-7; Putative GPI-anchored aspartic protease; member of the yapsin family of proteases involved in cell w growth and maintenance; located in the cytoplasm and endoplasmic reticulum; Belongs to the peptidase A1 family |
| EMT1 |
YNCD0025W |
Unknown |
| ATP22 |
YDR350C |
Specific translational activator for the mitochondrial ATP6 mRNA; Atp6p encodes a subunit of F1F0 ATP synthase; localized to the mitochondrial inner membrane |
| SBE2 |
YDR351W |
Protein required for bud growth; involved in transport of cell w components from the Golgi to the cell surface; SBE2 has a paralog, SBE22, that arose from the whole genome duplication |
| YPQ2 |
YDR352W |
Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; mutant phenotype is functiony complemented by rat PQLC2 vacuolar transporter; Belongs to the laat-1 family |
| TRR1 |
YDR353W |
Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication; Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family |
| TRP4 |
YDR354W |
Anthranilate phosphoribosyl transferase; transferase of the tryptophan biosynthetic pathway; catalyzes the phosphoribosylation of anthranilate; subject to the general control system of amino acid biosynthesis |
| SPC110 |
YDR356W |
Inner plaque spindle pole body (SPB) component; ortholog of human kendrin; gamma-tubulin sm complex (gamma-TuSC) receptor that interacts with Spc98p to recruit the complex to the nuclear side of the SPB, connecting nuclear microtubules to the SPB; promotes gamma-TuSC assembly and oligomerization to initiate microtubule nucleation; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner |
| CNL1 |
YDR357C |
Subunit of the BLOC-1 complex involved in endosomal maturation; interacts with Msb3p; null mutant is sensitive to drug inducing secretion of vacuolar cargo; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; Belongs to the BLOC1S4 family |
| GGA1 |
YDR358W |
ADP-ribosylation factor-binding protein GGA1; Golgi-localized protein with homology to gamma-adaptin; interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi; GGA1 has a paralog, GGA2, that arose from the whole genome duplication |
| EAF1 |
YDR359C |
Chromatin modification-related protein EAF1; Component of the NuA4 histone acetyltransferase complex; acts as a platform for assembly of NuA4 subunits into the native complex; required for initiation of pre-meiotic DNA replication, likely due to its requirement for expression of IME1; Belongs to the EAF1 family |
| BCP1 |
YDR361C |
Essential protein involved in nuclear export of Mss4p; Mss4p is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport; Belongs to the BCP1 family |
| TFC6 |
YDR362C |
Subunit of RNA polymerase III transcription initiation factor complex; one of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 |
| ESC2 |
YDR363W |
Sumo-like domain protein; prevents accumulation of toxic intermediates during replication-associated recombinational repair; roles in silencing, lifespan, chromatid cohesion and the intra-S-phase DNA damage checkpoint; RENi family member |
| YNCD0026W |
YNCD0026W |
Unknown |
| SEM1 |
YDR363W-A |
26S proteasome complex subunit SEM1; 19S proteasome regulatory particle lid subcomplex component; role in Ub-dependent proteolysis and proteasome stability; involved in TREX-2 mediated mRNA export, and in the prevention of transcription-associated genome instability; ubiquitinated by Nedd4-like E3-ligase, Rsp5p; human ortholog DSS1, a BRCA1 binding protein implicated in cancer, complements the yeast null; drives trinucleotide repeat expansion; protein abundance increases in response to DNA replication stress |
| CDC40 |
YDR364C |
Pre-mRNA-processing factor 17; Pre-mRNA splicing factor; important for catalytic step II of pre-mRNA splicing and plays a role in cell cycle progression, particularly at the G1/S phase transition; required for DNA synthesis during mitosis and meiosis; has WD repeats; thermosensitivity of the cdc40 null mutant is functiony complemented by a chimeric construct containing the N-terminal 156 amino acids of yeast Cdc40p fused to the C-terminal two thirds (297 amino acids) of human CDC40 |
| ESF1 |
YDR365C |
Pre-rRNA-processing protein ESF1; Nucleolar protein involved in pre-rRNA processing; depletion causes severely decreased 18S rRNA levels |
| MOR1 |
YDR366C |
Uncharacterized protein YDR366C; Putative protein of unknown function |
| KEI1 |
YDR367W |
Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationy processed by Kex2p; KEI1 is an essential gene |
| YPR1 |
YDR368W |
Putative reductase 1; NADPH-dependent aldo-keto reductase; reduces multiple substrates including 2-methylbutyraldehyde and D,L-glyceraldehyde, expression is induced by osmotic and oxidative stress; functiony redundant with other aldo-keto reductases; protein abundance increases in response to DNA replication stress; YPR1 has a paralog, GCY1, that arose from the whole genome duplication; human homolog AKR1B1 can complement yeast null mutant |
| XRS2 |
YDR369C |
Dna repair protein xrs2; Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling |
| DXO1 |
YDR370C |
Decapping and exoribonuclease protein 1; mRNA 5'-end-capping quality-control protein; has distributive, 5'-3' exoRNase activity; similar to Rai1p;; Belongs to the DXO/Dom3Z family |
| CTS2 |
YDR371W |
Sporulation-specific chitinase 2; Putative chitinase; functiony complements A. gossypii cts2 mutant sporulation defect |
| VPS74 |
YDR372C |
Vacuolar protein sorting-associated protein 74; Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33; Belongs to the GOLPH3/VPS74 family |
| FRQ1 |
YDR373W |
N-myristoylated calcium-binding protein; may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily; human NCS1 functiony complements the heat sensitivity of a frq1 ts mutant |
| PHO92 |
YDR374C |
Methylated RNA-binding protein 1; Posttranscriptional regulator of phosphate metabolism; facilitates PHO4 mRNA degradation by interacting with Pop2p; regulates PHO4 mRNA stability by binding to PHO4's 3'UTR in a phosphate-dependent manner; contains highly conserved YTH (YT521-B Homology) domain that exhibits RNA-binding activity; human homolog YTHDF2 can complement yeast null mutant |
| MUS81 |
YDR386W |
Subunit of structure-specific Mms4p-Mus81p endonuclease; cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; promotes template switching during break-induced replication (BIR), causing non-reciprocal translocations (NRTs); helix-hairpin-helix protein; phosphorylation of non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates function of Mms4p-Mus81p; Belongs to the XPF family |
| CIN10 |
YDR387C |
Mfs transporter, sp family, solute carrier family 2 (myo-inositol transporter), member 13; Probable metabolite transport protein YDR387C; Putative transporter; member of the sugar porter family; non-essential gene; overexpression results in elevated colony sectoring, an indicator of chromosomal instability |
| RVS167 |
YDR388W |
Reduced viability upon starvation protein 167; Calmodulin-binding actin-associated protein; roles in endocytic membrane tabulation and constriction, and exocytosis; N-BAR domain protein that interacts with Rvs161p to regulate actin cytoskeleton, endocytosis, and viability following starvation or osmotic stress; recruited to bud tips by Gyl1p and Gyp5p during polarized growth; homolog of mammalian amphiphysin |
| SAC7 |
YDR389W |
GTPase-activating protein SAC7; GTPase activating protein (GAP) for Rho1p; regulator of a Tor2p-mediated, Rho1p GTPase switch that controls organization of the actin cytoskeleton; negative regulator of the RHO1-PKC1-MAPK cell integrity (CWI) and membrane fluidity homeostasis signaling pathways; potential Cdc28p substrate; SAC7 has a paralog, BAG7, that arose from the whole genome duplication |
| UBA2 |
YDR390C |
Ubiquitin-activating enzyme E1-like; Subunit of heterodimeric nuclear SUMO activating enzyme E1 with Aos1p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability |
| SUF3 |
YNCD0027C |
Unknown |
| YDR391C |
YDR391C |
Uncharacterized protein YDR391C; Putative protein of unknown function; possibly involved in zinc homeostasis; Bdf1p-dependent transcription induced by salt stress; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus |
| SPT3 |
YDR392W |
Protein SPT3; Subunit of the SAGA and SAGA-like transcriptional regulatory complexes; interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters; relocalizes to the cytosol in response to hypoxia |
| SHE9 |
YDR393W |
Sensitive to high expression protein 9, mitochondrial; Protein required for normal mitochondrial morphology; mitochondrial inner membrane protein; may be involved in fission of the inner membrane; forms a homo-oligomeric complex; Belongs to the SHE9 family |
| RPT3 |
YDR394W |
ATPase of the 19S regulatory particle of the 26S proteasome; one of ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B |
| SXM1 |
YDR395W |
Importin beta SMX1; Nuclear transport factor (karyopherin); involved in protein transport between the cytoplasm and nucleoplasm; similar to Nmd5p, Cse1p, Lph2p, and the human cellular apoptosis susceptibility protein, CAS1; Belongs to the importin beta family |
| NCB2 |
YDR397C |
Subunit of a heterodimeric NC2 transcription regulator complex; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2beta; complex also includes Bur6p |
| UTP5 |
YDR398W |
U3 sm nucleolar RNA-associated protein 5; Subunit of U3-containing Sm Subunit (SSU) processome complex; involved in production of 18S rRNA and assembly of sm ribosomal subunit; Belongs to the UTP5 family |
| HPT1 |
YDR399W |
Dimeric hypoxanthine-guanine phosphoribosyltransferase; catalyzes the transfer of the phosphoribosyl portion of 5-phosphoribosyl-alpha-1-pyrophosphate to a purine base (either guanine or hypoxanthine) to form pyrophosphate and a purine nucleotide (either guanosine monophosphate or inosine monophosphate); mutations in the human homolog HPRT1 can cause Lesch-Nyhan syndrome and Kelley-Seegmiller syndrome |
| URH1 |
YDR400W |
Uridine nucleosidase (uridine-cytidine N-ribohydrolase); cleaves N-glycosidic bonds in nucleosides; involved in the pyrimidine salvage and nicotinamide riboside salvage pathways; Belongs to the IUNH family |
| DIT2 |
YDR402C |
Cytochrome P450-DIT2; N-formyltyrosine oxidase; sporulation-specific microsomal enzyme involved in the production of N,N-bisformyl dityrosine required for spore w maturation, homologous to cytochrome P-450s; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum and vacuole respectively |
| DIT1 |
YDR403W |
Sporulation-specific enzyme required for spore w maturation; involved in the production of a soluble LL-dityrosine-containing precursor of the spore w; transcripts accumulate at the time of prospore enclosure |
| RPB7 |
YDR404C |
RNA polymerase II subunit B16; forms dissociable heterodimer with Rpb4p; Rpb4/7 dissociates from RNAPII as Ser2 CTD phosphorylation increases; Rpb4/7 regulates cellular lifespan via mRNA decay process; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex, export of mRNA to cytoplasm under stress conditions; also involved in translation initiation |
| MRP20 |
YDR405W |
Mitochondrial 54s ribosomal protein yml41; Mitochondrial ribosomal protein of the large subunit |
| PDR15 |
YDR406W |
ATP-dependent permease PDR15; Plasma membrane ATP binding cassette (ABC) transporter; multidrug transporter and general stress response factor implicated in cellular detoxification; regulated by Pdr1p, Pdr3p and Pdr8p; promoter contains a PDR responsive element; PDR15 has a paralog, PDR5, that arose from the whole genome duplication; Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily |
| TRS120 |
YDR407C |
Trafficking protein particle complex II-specific subunit 120; Component of transport protein particle (TRAPP) complex II; TRAPPII is a multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating intra-Golgi and endosome-Golgi traffic; Belongs to the TRS120 family |
| ADE8 |
YDR408C |
Phosphoribosylglycinamide formyltransferase; Phosphoribosyl-glycinamide transformylase; catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway |
| SIZ1 |
YDR409W |
SUMO E3 ligase; promotes attachment of sm ubiquitin-related modifier sumo (Smt3p) to primarily cytoplasmic proteins; regulates Rsp5p ubiquitin ligase activity and is in turn itself regulated by Rsp5p; required for sumoylation of septins and histone H3 variant Cse4p, a prerequisite for STUbL-mediated Ub-dependent degradation; localizes to the septin ring; acts as an adapter between E2, Ubc9p and substrates; tends to compensate for survival of DNA damage in absence of Nfi1p |
| STE14 |
YDR410C |
Protein-S-isoprenylcysteine O-methyltransferase; Farnesyl cysteine-carboxyl methyltransferase; mediates the carboxyl methylation step during C-terminal CAAX motif processing of a-factor and RAS proteins in the endoplasmic reticulum, localizes to the ER membrane; Belongs to the class VI-like SAM-binding methyltransferase superfamily. Isoprenylcysteine carboxyl methyltransferase family |
| DFM1 |
YDR411C |
DER1-like family member protein 1; Endoplasmic reticulum (ER) localized protein; involved in ER-associated protein degradation (ERAD), ER stress, and homeostasis; interacts with components of ERAD-L and ERAD-C and Cdc48p; derlin-like family member similar to Der1p; Belongs to the derlin family |
| RRP17 |
YDR412W |
Ribosomal RNA-processing protein 17; Component of the pre-60S pre-ribosomal particle; required for cell viability under standard (aerobic) conditions but not under anaerobic conditions; exonuclease required for 5' end processing of pre-60S ribosomal RNA |
| ERD1 |
YDR414C |
Protein ERD1; Predicted membrane protein required for lumenal ER protein retention; mutants secrete the endogenous ER protein, BiP (Kar2p) |
| YDR415C |
YDR415C |
Probable aminopeptidase YDR415C; Putative aminopeptidase; SWAT-GFP fusion protein localizes to the endoplasmic reticulum and vacuole, while mCherry fusion localizes to the vacuole |
| SYF1 |
YDR416W |
Pre-mRNA-splicing factor SYF1; Member of the NineTeen Complex (NTC); that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; null mutant has splicing defect and arrests in G2/M; relocalizes to the cytosol in response to hypoxia; homologs in human and C. elegans |
| RPL12B |
YDR418W |
Ribosomal 60S subunit protein L12B; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12B has a paralog, RPL12A, that arose from the whole genome duplication |
| RAD30 |
YDR419W |
DNA polymerase eta; involved in translesion synthesis during post-replication repair; catalyzes the synthesis of DNA opposite cyclobutane pyrimidine dimers and other lesions; involved in formation of post-replicative damage-induced genome-wide cohesion; may also have a role in protection against mitochondrial mutagenesis; mutations in human pol eta are responsible for XPV |
| YNCD0028W |
YNCD0028W |
Unknown |
| HKR1 |
YDR420W |
Signaling mucin HKR1; Mucin family member that functions as an osmosensor in the HOG pathway; large, highly glycosylated protein that functions redundantly with Msb2p in Sho1p-mediated osmostresss induction of the HOG signaling pathway; cytoplasmic domain interacts with Ahk1p a scaffold protein that prevents cross talk with the Kss1p MAPK of the filamentous growth pathway; mutant displays defects in beta-1,3 glucan synthesis and bud site selection; Belongs to the HKR1/MSB2 family |
| ARO80 |
YDR421W |
Transcriptional activator aro80; Zinc finger transcriptional activator of the Zn2Cys6 family; activates transcription of aromatic amino acid catabolic genes in the presence of aromatic amino acids |
| SIP1 |
YDR422C |
Alternate beta-subunit of the Snf1p kinase complex; may confer substrate specificity; vacuolar protein containing KIS (Kinase-Interacting Sequence) and ASC (Association with Snf1 kinase Complex) domains involved in protein interactions |
| CAD1 |
YDR423C |
AP-1-like basic leucine zipper (bZIP) transcriptional activator; involved in stress responses, iron metabolism, and pleiotropic drug resistance; controls a set of genes involved in stabilizing proteins; binds consensus sequence TTACTAA; CAD1 has a paralog, YAP1, that arose from the whole genome duplication |
| DYN2 |
YDR424C |
Cytoplasmic light chain dynein, microtubule motor protein; required for intracellular transport and cell division; involved in mitotic spindle positioning; forms complex with dynein intermediate chain Pac11p that promotes Dyn1p homodimerization, potentiates motor processivity; Dyn2p-Pac11p complex important for interaction of dynein motor complex with dynactin complex; acts as molecular glue to dimerize, stabilize Nup82-Nsp1-Nup159 complex module of cytoplasmic pore filaments |
| SNX41 |
YDR425W |
Sorting nexin; involved in the retrieval of late-Golgi SNAREs from the post-Golgi endosome to the trans-Golgi network; interacts with Snx4p |
| RPN9 |
YDR427W |
Non-ATPase regulatory subunit of the 26S proteasome; similar to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects; protein abundance increases in response to DNA replication stress; relocalizes to the cytosol in response to hypoxia |
| BNA7 |
YDR428C |
Formylkynurenine formamidase; involved in the de novo biosynthesis of NAD from tryptophan via kynurenine |
| TIF35 |
YDR429C |
eIF3g subunit of the eukaryotic translation initiation factor 3 (eIF3); subunit of the core complex of eIF3; is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation; eIF3 is also involved in programmed stop codon readthrough |
| CYM1 |
YDR430C |
Mitochondrial presequence protease; Lysine-specific metoprotease of the pitrilysin family; metoprotease of the intermembrane space; degrades proteins and presequence peptides cleaved from imported proteins; required for normal mitochondrial morphology |
| NPL3 |
YDR432W |
Transcription initiation factor tfiid subunit 15; Nucleolar protein 3; RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; represses translation initiation by binding eIF4G; required for pre-mRNA splicing; interacts with E3 ubiquitin ligase Bre1p, linking histone ubiquitination to mRNA processing; may have role in telomere maintenance; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in cytoplasm; protein abundance increases in response to DNA replication stress |
| GPI17 |
YDR434W |
GPI transamidase component GPI17; Transmembrane protein; subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-S homolog; Belongs to the PIGS family |
| PPM1 |
YDR435C |
Carboxyl methyltransferase; methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits; required for methionine to inhibit autophagy and promote growth |
| PPZ2 |
YDR436W |
Serine/threonine-protein phosphatase PP-Z2; Serine/threonine protein phosphatase Z, isoform of Ppz1p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance |
| GPI19 |
YDR437W |
Subunit of GPI-GlcNAc transferase involved in synthesis of GlcNAc-PI; N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI) is the first intermediate in glycosylphosphatidylinositol (GPI) anchor synthesis; shares similarity with mammalian PIG-P; Belongs to the GPI19 family |
| THI74 |
YDR438W |
Mitochondrial transporter repressible by thiamine; THI74 has a paralog, YML018C, that arose from the whole genome duplication; shows sequence homology to human gene SLC35F3, a thiamine transporter implicated in hypertension |
| LRS4 |
YDR439W |
Monopolin complex subunit LRS4; Nucleolar protein that forms a complex with Csm1p; and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation |
| DOT1 |
YDR440W |
Histone-lysine N-methyltransferase, H3 lysine-79 specific; Nucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response |
| APT2 |
YDR441C |
Potential adenine phosphoribosyltransferase; encodes a protein with similarity to adenine phosphoribosyltransferase, but artificiy expressed protein exhibits no enzymatic activity; APT2 has a paralog, APT1, that arose from the whole genome duplication |
| SSN2 |
YDR443C |
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation |
| YDR444W |
YDR444W |
Putative lipase YDR444W; Putative hydrolase acting on ester bonds; Belongs to the putative lipase ROG1 family |
| YNCD0029C |
YNCD0029C |
Unknown |
| ECM11 |
YDR446W |
Meiosis-specific protein; component of the Synaptonemal Complex (SC) along with Gmc2p; required for efficient crossover formation and for the efficient loading of the SC transverse filament protein, Zip1p; is SUMOlytaed in a Gmc2p manner, and SUMOylation is required for its function in meiosis; GFP fusion protein is present in discrete clusters in the nucleus throughout mitosis; may be involved in maintaining chromatin structure |
| RPS17B |
YDR447C |
Ribosomal protein 51 (rp51) of the sm (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17B has a paralog, RPS17A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress |
| ADA2 |
YDR448W |
Chromatin-binding transcription regulator ada2; Transcriptional adapter 2; Transcription coactivator; component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes |
| UTP6 |
YDR449C |
Snorna-binding rrna-processing protein utp6; Nucleolar protein; component of the sm subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA |
| RPS18A |
YDR450W |
Protein component of the sm (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18A has a paralog, RPS18B, that arose from the whole genome duplication; protein increases in abundance and relocalizes from cytoplasm to nuclear foci upon DNA replication stress |
| YHP1 |
YDR451C |
Homeobox protein YHP1; Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication |
| PPN1 |
YDR452W |
Endopolyphosphatase; Dual endo- and exopolyphosphatase with a role in phosphate metabolism; acts as both an endopolyphosphatase cleaving long chains of polyphosphate distributively to generate shorter polymer chains and as an exopolyphosphatase catalyzing the hydrolysis of terminal phosphate from polyphosphate; localizes to the vacuole, nucleus and cytosol; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress |
| TSA2 |
YDR453C |
Peroxiredoxin TSA2; Stress inducible cytoplasmic thioredoxin peroxidase; cooperates with Tsa1p in the removal of reactive oxygen, nitrogen and sulfur species using thioredoxin as hydrogen donor; deletion enhances the mutator phenotype of tsa1 mutants; protein abundance increases in response to DNA replication stress; TSA2 has a paralog, TSA1, that arose from the whole genome duplication; Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily |
| GUK1 |
YDR454C |
Guanylate kinase; converts GMP to GDP; required for growth and mannose outer chain elongation of cell w N-linked glycoproteins |
| NHX1 |
YDR456W |
Solute carrier family 9 (sodium/hydrogen exchanger), member 6/7; Endosomal/prevacuolar sodium/hydrogen exchanger; Na+/H+ and K+/H+ exchanger; required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion; ortholog of human NHE9, which is linked to autism; Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family |
| TOM1 |
YDR457W |
E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase; Belongs to the UPL family. TOM1/PTR1 subfamily |
| HEH2 |
YDR458C |
Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle; HEH2 has a paralog, SRC1, that arose from the whole genome duplication |
| PFA5 |
YDR459C |
Palmitoyltransferase with autoacylation activity; likely functions in pathway(s) outside Ras; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; Belongs to the DHHC palmitoyltransferase family. PFA5 subfamily |
| TFB3 |
YDR460W |
Subunit of TFIIH and nucleotide excision repair factor 3 complexes; involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit |
| MFA1 |
YDR461W |
Mating pheromone a-factor; made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA2 |
| CMI8 |
YDR461C-A |
Uncharacterized protein YDR461C-A; Putative protein of unknown function |
| MRPL28 |
YDR462W |
Mitochondrial 54s ribosomal protein yml28; Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress |
| STP1 |
YDR463W |
Transcription factor; contains a N-terminal regulatory motif (RI) that acts as a cytoplasmic retention determinant and as an Asi dependent degron in the nucleus; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication |
| SPP41 |
YDR464W |
Protein of unknown function; involved in negative regulation of expression of spliceosome components PRP4 and PRP3; relocalizes to the cytosol in response to hypoxia |
| RMT2 |
YDR465C |
Arginine N5 methyltransferase; methylates ribosomal protein Rpl12 (L12) on Arg67; relative distribution to the nucleus increases upon DNA replication stress |
| PKH3 |
YDR466W |
3-phosphoinositide dependent protein kinase-1; Serine/threonine-protein kinase PKH3; Protein kinase with similarity to mammalian PDK1 and yeast Pkh1p/Phk2p; yeast Pkh1p and Pkh2p are two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant |
| TLG1 |
YDR468C |
Essential t-SNARE that mediates fusion of vesicles with the late Golgi; forms a complex with Tlg2p and Vti1p; mediates fusion of endosome-derived vesicles with the late Golgi; binds the docking complex VFT (Vps fifty-three) through interaction with Vps51p; Belongs to the syntaxin family |
| SDC1 |
YDR469W |
COMPASS component SDC1; Subunit of the COMPASS (Set1C) complex; COMPASS methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30 |
| UGO1 |
YDR470C |
Outer membrane component of the mitochondrial fusion machinery; binds to Fzo1p and Mgm1p to link these two GTPases during mitochondrial fusion; involved in fusion of both the outer and inner membranes; facilitates dimerization of Fzo1p during fusion; import into the outer membrane is mediated by Tom70p and Mim1p; has similarity to carrier proteins but likely not a transporter; similar to human SLC25A46 implicated in optic atroprophy spectrum disorder |
| RPL27B |
YDR471W |
Ribosomal 60S subunit protein L27B; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27B has a paralog, RPL27A, that arose from the whole genome duplication |
| SNR13 |
YNCD0030W |
Unknown |
| TRS31 |
YDR472W |
Trafficking protein particle complex subunit 31; Core component of transport protein particle (TRAPP) complexes I-III; TRAPP complexes are related multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating ER-Golgi traffic (TRAPPI), intra-Golgi traffic (TRAPPII), endosome-Golgi traffic (TRAPPII and III) and autophagy (TRAPPIII) |
| PRP3 |
YDR473C |
U4/U6 sm nuclear ribonucleoprotein PRP3; Splicing factor; component of the U4/U6-U5 snRNP complex |
| JIP4 |
YDR475C |
Uncharacterized protein JIP4; Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence; JIP4 has a paralog, YOR019W, that arose from the whole genome duplication |
| YDR476C |
YDR476C |
Uncharacterized protein YDR476C; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene |
| SNF1 |
YDR477W |
AMP-activated S/T protein kinase; forms a complex with Snf4p and members of the Sip1p/Sip2p/Gal83p family; required for transcription of glucose-repressed genes, thermotolerance, sporulation, and peroxisome biogenesis; regulates nucleocytoplasmic shuttling of Hxk2p; regulates filamentous growth and acts as a non-canonical GEF, activating Arf3p during invasive growth; SUMOylation by Mms21p inhibits its function and targets Snf1p for destruction via the Slx5-Slx8 Ub ligase |
| SNM1 |
YDR478W |
Ribonuclease MRP complex subunit; ribonuclease (RNase) MRP cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP |
| PEX29 |
YDR479C |
ER-resident protein involved in peroxisomal biogenesis; ER-localized protein that associates with peroxisomes; interacts with Pex30p and reticulons Rtn1p and Yop1p to regulate peroxisome biogenesis from the ER; role in peroxisomal-destined vesicular flow from the ER; regulates peroxisomal size, number and distribution; Pex28p and Pex29p may act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress |
| DIG2 |
YDR480W |
Down-regulator of invasive growth 2; MAP kinase-responsive inhibitor of the Ste12p transcription factor; involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p; DIG2 has a paralog, DIG1, that arose from the whole genome duplication |
| PHO8 |
YDR481C |
Repressible vacuolar alkaline phosphatase; regulated by levels of Pi and by Pho4p, Pho9p, Pho80p, Pho81p and Pho85p; dephosphorylates phosphotyrosyl peptides; contributes to NAD+ metabolism by producing nicotinamide riboside from NMN |
| CWC21 |
YDR482C |
Pre-mRNA-splicing factor CWC21; Protein involved in RNA splicing by the spliceosome; component of a complex containing Cef1p; interacts geneticy with ISY1 and BUD13; may bind RNA; has similarity to S. pombe Cwf21p; Belongs to the CWC21 family |
| KRE2 |
YDR483W |
Glycolipid 2-alpha-mannosyltransferase; Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication |
| VPS52 |
YDR484W |
Vacuolar protein sorting-associated protein 52; Component of the GARP (Golgi-associated retrograde protein) complex; GARP is required for the recycling of proteins from endosomes to the late Golgi, and for mitosis after DNA damage induced checkpoint arrest; involved in localization of actin and chitin; members of the GARP complex are Vps51p-Vps52p-Vps53p-Vps54p |
| VPS72 |
YDR485C |
Vacuolar protein sorting-associated protein 72; Htz1p-binding component of the SWR1 complex; exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; may function as a lock that prevents removal of H2AZ from nucleosomes; required for vacuolar protein sorting |
| VPS60 |
YDR486C |
Protein involved in late endosome to vacuole transport; cytoplasmic and vacuolar membrane protein; required for normal filament maturation during pseudohyphal growth; may function in targeting cargo proteins for degradation; interacts with Vta1p; Belongs to the SNF7 family |
| RIB3 |
YDR487C |
3,4-dihydroxy-2-butanone-4-phosphate synthase RIB3; 3,4-dihydroxy-2-butanone-4-phosphate synthase (DHBP synthase); required for riboflavin biosynthesis from ribulose-5-phosphate, also has an unrelated function in mitochondrial respiration |
| PAC11 |
YDR488C |
WD repeat-containing protein PAC11; Dynein intermediate chain, microtubule motor protein; required for intracellular transport and cell division; acts in cytoplasmic dynein pathway; forms complex with dynein light chain Dyn2p that promotes Dyn1p homodimerization and potentiates motor processivity; Dyn2p-Pac11p complex is also important for interaction of dynein motor complex with dynactin complex; forms cortical cytoplasmic microtubule capture site with Num1p; essential in the absence of CIN8 |
| SLD5 |
YDR489W |
Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p); complex is localized to DNA replication origins and implicated in assembly of the DNA replication machinery; Belongs to the GINS4/SLD5 family |
| PKH1 |
YDR490C |
3-phosphoinositide dependent protein kinase-1; Serine/threonine-protein kinase PKH1; Serine/threonine protein kinase; involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell w integrity; contains a PH-like domain; redundant with Pkh2p; PKH1 has a paralog, PKH2, that arose from the whole genome duplication; Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDPK1 subfamily |
| IZH1 |
YDR492W |
ADIPOR-like receptor IZH1; Membrane protein involved in zinc ion homeostasis; member of the four-protein IZH family; transcription is regulated directly by Zap1p, expression induced by zinc deficiency and fatty acids; deletion increases sensitivity to elevated zinc; IZH1 has a paralog, IZH4, that arose from the whole genome duplication |
| MZM1 |
YDR493W |
Mitochondrial zinc maintenance protein 1, mitochondrial; Protein required for assembly of the cytochrome bc(1) complex; acts as a chaperone for Rip1p and facilitates its insertion into the complex at a late stage of assembly; localized to the mitochondrial matrix; null mutant exhibits a respiratory growth defect and reduced mitochondrial zinc levels, which is characteristic of mutations affecting bc(1) complex assembly; member of the LYR protein family; human LYRM7 is a functional ortholog |
| RSM28 |
YDR494W |
37S ribosomal protein RSM28, mitochondrial; Mitochondrial ribosomal protein of the sm subunit; genetic interactions suggest a possible role in promoting translation initiation |
| VPS3 |
YDR495C |
Vacuolar protein sorting-associated protein 3; Component of CORVET membrane tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase |
| PUF6 |
YDR496C |
Pumilio-homology domain protein; binds the 3' UTR of ASH1 mRNA and represses its translation, resulting in proper asymmetric localization of ASH1 mRNA; required at post-transcriptional step for efficient retrotransposition; absence results in decreased Ty1 Gag:GFP protein levels; co-sediments with the 60S ribosomal subunit and is required for its biogenesis; Belongs to the PUF6 family |
| ITR1 |
YDR497C |
Myo-inositol transporter; member of the sugar transporter superfamily; expression is repressed by inositol and choline via Opi1p and derepressed via Ino2p and Ino4p; relative distribution to the vacuole increases upon DNA replication stress; ITR1 has a paralog, ITR2, that arose from the whole genome duplication |
| SEC20 |
YDR498C |
Membrane glycoprotein v-SNARE; involved in retrograde transport from the Golgi to the endoplasmic reticulum (ER); required for N- and O-glycosylation in the Golgi but not in the ER and for efficient nuclear fusion during mating; mediates Sey1p-independent homotypic ER fusion; interacts with the Dsl1p complex through Tip20p; Belongs to the SEC20 family |
| LCD1 |
YDR499W |
Essential protein required for the DNA integrity checkpoint pathways; interacts physicy with Mec1p; putative homolog of S. pombe Rad26 and human ATRIP; forms nuclear foci upon DNA replication stress |
| RPL37B |
YDR500C |
Ribosomal 60S subunit protein L37B; required for processing of 27SB pre-rRNA and formation of stable 66S assembly intermediates; protein abundance increases in response to DNA replication stress; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37B has a paralog, RPL37A, that arose from the whole genome duplication |
| PLM2 |
YDR501W |
Protein PLM2; Putative transcription factor, contains Forkhead Associated domain; found associated with chromatin; target of SBF transcription factor; induced in response to DNA damaging agents and deletion of telomerase; PLM2 has a paralog, TOS4, that arose from the whole genome duplication; Belongs to the PLM2/TOS4 family |
| SAM2 |
YDR502C |
S-adenosylmethionine synthase 2; S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; comparative analysis suggests that a mitochondriy targeted form may result from translation starting at a non-canonical codon upstream of the annotated start codon; Belongs to the AdoMet synthase family |
| LPP1 |
YDR503C |
Lipid phosphate phosphatase; catalyzes Mg(2+)-independent dephosphorylation of phosphatidic acid (PA), lysophosphatidic acid, and diacylglycerol pyrophosphate; involved in control of the cellular levels of phosphatidylinositol and PA |
| SPG3 |
YDR504C |
Stationary phase protein 3; Protein required for high temperature survival during stationary phase; not required for growth on nonfermentable carbon sources; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum |
| PSP1 |
YDR505C |
Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication |
| GMC1 |
YDR506C |
Putative multicopper oxidase GMC1; Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; possible membrane-localized protein; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum and vacuole respectively |
| YNCD0031C |
YNCD0031C |
Unknown |
| GIN4 |
YDR507C |
Serine/threonine-protein kinase GIN4; Protein kinase involved in bud growth and assembly of the septin ring; proposed to have kinase-dependent and kinase-independent activities; undergoes autophosphorylation; similar to Hsl1p; GIN4 has a paralog, KCC4, that arose from the whole genome duplication; Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NIM1 subfamily |
| GNP1 |
YDR508C |
High-affinity glutamine permease; also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids; GNP1 has a paralog, AGP1, that arose from the whole genome duplication |
| SMT3 |
YDR510W |
Sm ubiquitin-related modifier; Ubiquitin-like protein of the SUMO family; conjugated to lysine residues of target proteins; associates with transcriptiony active genes; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; human homolog SUMO1 can complement yeast null mutant |
| SDH7 |
YDR511W |
Mitochondrial protein involved in assembly of succinate dehydrogenase; has a role in maturation of the Sdh2p subunit; localized to the mitochondrial intermembrane space; required for acetate utilization and gluconeogenesis; mutation in Drosophila ortholog SDHAF3 causes reduced succinate dehydrogenase activity and neuronal and muscular dysfunction; member of the LYR protein family |
| EMI1 |
YDR512C |
Early meiotic induction protein 1; Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1, also required for sporulation; contains twin cysteine-x9-cysteine motifs; deletion affects mitochondrial morphology |
| GRX2 |
YDR513W |
Glutaredoxin-2, mitochondrial; Cytoplasmic glutaredoxin; thioltransferase, glutathione-dependent disulfide oxidoreductase involved in maintaining redox state of target proteins, also exhibits glutathione peroxidase activity, expression induced in response to stress; GRX2 has two in-frame start codons resulting in a shorter isoform that is retained in the cytosol and a longer form translocated to the mitochondrial matrix; GRX2 has a paralog, GRX1, that arose from the whole genome duplication |
| YDR514C |
YDR514C |
Uncharacterized protein YDR514C; Protein of unknown function that localizes to mitochondria; overexpression affects endocytic protein trafficking; YDR514C has a paralog, GFD2, that arose from the whole genome duplication |
| SLF1 |
YDR515W |
RNA binding protein that associates with polysomes; may be involved in regulating mRNA translation; involved in the copper-dependent mineralization of copper sulfide complexes on cell surface in cells cultured in copper salts; SLF1 has a paralog, SRO9, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress |
| EMI2 |
YDR516C |
Putative glucokinase-2; Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress |
| GRH1 |
YDR517W |
GRASP65 homolog protein 1; Acetylated cis-Golgi protein, involved in ER to Golgi transport; homolog of human GRASP65; forms a complex with the coiled-coil protein Bug1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes; protein abundance increases in response to DNA replication stress |
| EUG1 |
YDR518W |
Protein disulfide-isomerase EUG1; Protein disulfide isomerase of the endoplasmic reticulum lumen; EUG1 has a paralog, PDI1, that arose from the whole genome duplication; function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER |
| FPR2 |
YDR519W |
Membrane-bound peptidyl-prolyl cis-trans isomerase (PPIase); binds to the drugs FK506 and rapamycin; expression pattern suggests possible involvement in ER protein trafficking; relocalizes from nucleus to vacuole upon DNA replication stress; mutation is functiony complemented by human FKBP2 |
| URC2 |
YDR520C |
Uracil catabolism protein 2; Putative Zn(II)2Cys6 motif containing transcription factor; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; similar to S. kluyveri Urc2p involved in uracil catabolism |
| SPS2 |
YDR522C |
Protein expressed during sporulation; SPS2 has a paralog, SPS22, that arose from the whole genome duplication; redundant with Sps22p for organization of the beta-glucan layer of the spore w; S. pombe ortholog is a spore w component |
| SPS1 |
YDR523C |
Sporulation-specific protein 1; Putative protein serine/threonine kinase; localizes to the nucleus and cytoplasm; required for efficient spore packaging, prospore membrane development and closure and localization of enzymes involved in spore w synthesis; interacts with and required for Ssp1p phosphorylation and turnover; member of the GCKIII subfamily of STE20 kinases; multiply phosphorylated on S/T residues; interacts with 14-3-3 proteins, Bmh1p and Bmh2p; expressed at the end of meiosis |
| AGE1 |
YDR524C |
ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector; involved in the secretory and endocytic pathways; contains C2C2H2 cysteine/histidine motif |
| YDR524W-C |
YDR524W-C |
Uncharacterized protein YDR524W-C; Putative protein of unknown function; sm ORF identified by SAGE; deletion strains are moderately sensitive to the radiomimetic drug bleomycin |
| YDR524C-B |
YDR524C-B |
Uncharacterized protein YDR524C-B; Putative protein of unknown function; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum; YDR524C-B has a paralog, YCL048W-A, that arose from the whole genome duplication |
| API2 |
YDR525W |
Uncharacterized protein API2; Putative protein of unknown function; conserved among S. cerevisiae strains; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology |
| SNA2 |
YDR525W-A |
Protein of unknown function; has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; Belongs to the UPF0057 (PMP3) family |
| RBA50 |
YDR527W |
Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1 |
| SNR84 |
YNCD0032C |
Unknown |
| HLR1 |
YDR528W |
Protein involved in regulation of cell w composition and integrity; also involved in cell w response to osmotic stress; overproduction suppresses a lysis sensitive PKC mutation; HLR1 has a paralog, LRE1, that arose from the whole genome duplication |
| QCR7 |
YDR529C |
Subunit 7 of ubiquinol cytochrome-c reductase (Complex III); Complex III is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly; Belongs to the UQCRB/QCR7 family |
| APA2 |
YDR530C |
Sulfate adenylyltransferase (adp) / atp adenylyltransferase; Diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase II; AP4A phosphorylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; APA2 has a paralog, APA1, that arose from the whole genome duplication |
| CAB1 |
YDR531W |
Pantothenate kinase, ATP:D-pantothenate 4'-phosphotransferase; catalyzes the first committed step in the universal biosynthetic pathway for synthesis of coenzyme A (CoA); transcriptiony regulated by Upc2p via a sterol response element |
| KRE28 |
YDR532C |
Cytoplasmic fmr1 interacting protein; Spindle pole body component KRE28; Subunit of a kinetochore-microtubule binding complex; complex bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components; complex also includes Spc105p; modified by sumoylation |
| HSP31 |
YDR533C |
Glutathione-independent glyoxalase HSP31; Methylglyoxalase that converts methylglyoxal to D-lactate; involved in oxidative stress resistance, diauxic shift, and stationary phase survival; has similarity to E. coli Hsp31 and C. albicans Glx3p; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease and cancer; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress; Belongs to the peptidase C56 family. HSP31-like subfamily |
| FIT1 |
YDR534C |
Facilitator of iron transport 1; Mannoprotein that is incorporated into the cell w; incorporated via a glycosylphosphatidylinositol (GPI) anchor; involved in the retention of siderophore-iron in the cell w |
| STL1 |
YDR536W |
Sugar transporter STL1; Glycerol proton symporter of the plasma membrane; subject to glucose-induced inactivation, strongly but transiently induced when cells are subjected to osmotic shock |
| PAD1 |
YDR538W |
Flavin prenyltransferase PAD1, mitochondrial; Phenylacrylic acid decarboxylase; confers resistance to cinnamic acid, decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives; also has mRNA binding activity; homolog of E. coli UbiX; co-overproduction of Pad1p and Fdc1p greatly increases cinnamic acid decarboxylase activity |
| FDC1 |
YDR539W |
Ferulic acid decarboxylase, also active on p-coumaric acid; essential for decarboxylation of aromatic carboxylic acids to corresponding vinyl derivatives; co-overproduction of Pad1p and Fdc1p greatly increases cinnamic acid decarboxylase activity; structure implicates Glu285 as the general base in the nonoxidative decarboxylation reaction catalyzed by Fdc1p; homolog of E. coli UbiD; GFP-fusion protein localizes to cytoplasm |
| IRC4 |
YDR540C |
Uncharacterized protein IRC4; Protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus |
| YDR541C |
YDR541C |
Putative uncharacterized oxidoreductase YDR541C; Aldehyde reductase; substrates include both aromatic and aliphatic aldehydes; uses NADPH as cofactor; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum and vacuole respectively; Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily |
| MAL11 |
YGR289C |
General alpha-glucoside permease; High-affinity maltose transporter (alpha-glucoside transporter); inducible; encoded in the MAL1 complex locus; broad substrate specificity that includes maltotriose; required for isomaltose utilization |
| YCR108C |
YCR108C |
Uncharacterized protein YCR108C; Putative protein of unknown function; identified by fungal homology and RT-PCR |
| YEL073C |
YEL073C |
Uncharacterized protein YEL073C; Putative protein of unknown function; located adjacent to ARS503 and the telomere on the left arm of chromosome V; regulated by inositol/choline |
| RMD6 |
YEL072W |
Sporulation protein rmd6; Required for sporulation. Required for meiotic nuclear division |
| DLD3 |
YEL071W |
D-2-hydroxyglutarate--pyruvate transhydrogenase DLD3; 2-hydroxyglutarate transhydrogenase, and minor D-lactate dehydrogenase; converts D-2-hydroxyglutarate (D-2HG), an oncometabolite, to alpha-ketoglutarate in the presence of FAD, with concomitant reduction of pyruvate to D-lactate; minor lactate dehydrogenase activity; component of the retrograde regulon that consists of genes whose expression are stimulated by damage to mitochondria and reduced in cells grown with glutamate as the sole nitrogen source; located in the cytoplasm |
| DSF1 |
YEL070W |
Mannitol dehydrogenase; deletion suppresses mutation of mpt5; DSF1 has a paralog, MAN2, that arose from a segmental duplication |
| HXT13 |
YEL069C |
Mfs transporter, sp family, sugar:h+ symporter; Hexose transporter HXT13; Putative transmembrane polyol transporter; supports growth on and uptake of mannitol and sorbitol with moderate affinity when overexpressed in a strain deleted for hexose family members; minor hexose transport activity when overexpressed in a similar strain; induced by non-fermentable carbon sources; induced in low glucose, repressed in high glucose; HXT13 has a paralog, HXT17, that arose from a segmental duplication |
| YEL068C |
YEL068C |
Uncharacterized protein YEL068C; Protein of unknown function; expressed at both mRNA and protein levels; SWAT-GFP and seamless-GFP fusion proteins localize to the endoplasmic reticulum and mCherry fusion protein localizes to the vacuole |
| YEL067C |
YEL067C |
Uncharacterized protein YEL067C; Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies |
| HPA3 |
YEL066W |
D-amino-acid N-acetyltransferase HPA3; D-Amino acid N-acetyltransferase that detoxifies D-amino acids; catalyzes N-acetylation of D-amino acids through ordered bi-bi mechanism in which acetyl-CoA is first substrate bound and CoA is last product liberated; acetylates histones and polyamines, also autoacetylates |
| SIT1 |
YEL065W |
Mfs transporter, sit family, siderophore-iron:h+ symporter; Siderophore iron transporter 1; Ferrioxamine B transporter; member of the ARN family of transporters that specificy recognize siderophore-iron chelates; transcription is induced during iron deprivation and diauxic shift; potentiy phosphorylated by Cdc28p |
| AVT2 |
YEL064C |
Vacuolar amino acid transporter 2; Putative transporter; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters |
| CAN1 |
YEL063C |
Yeast amino acid transporter; Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication |
| NPR2 |
YEL062W |
Nitrogen permease regulator 2; Subunit of the Iml1p/SEACIT complex; SEACIT (Iml1p-Npr2p-Npr3p) is a subcomplex of the SEA complex, a coatomer-related complex that associates dynamicy with the vacuole; Npr2p may have a structural or regulatory role, supporting Iml1p function as a GAP for the Rag family GTPase Gtr1p, and resulting in inhibition of TORC1 signaling in response to amino acid deprivation; SEACIT is required for non-nitrogen-starvation-induced autophagy; homolog of human tumor suppressor NPRL2 |
| CIN8 |
YEL061C |
Kinesin-like protein CIN8; Kinesin motor protein; involved in mitotic spindle assembly and chromosome segregation |
| PRB1 |
YEL060C |
Cerevisin; Vacuolar proteinase B (yscB) with H3 N-terminal endopeptidase activity; serine protease of the subtilisin family; involved in protein degradation in the vacuole and required for full protein degradation during sporulation; activity inhibited by Pbi2p; protein abundance increases in response to DNA replication stress; PRB1 has a paralog, YSP3, that arose from the whole genome duplication |
| SOM1 |
YEL059C-A |
Protein SOM1, mitochondrial; Subunit of the mitochondrial inner membrane peptidase (IMP); IMP is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates; contains twin cysteine-x9-cysteine motifs |
| HHY1 |
YEL059W |
Hypersensitivity to hygromycin-B protein 1; Dubious open reading frame unlikely to encode a functional protein; mutant is hypersensitive to hygromycin B indicative of defects in vacuolar trafficking |
| PCM1 |
YEL058W |
Phosphoacetylglucosamine mutase pcm1; Essential N-acetylglucosamine-phosphate mutase; converts GlcNAc-6-P to GlcNAc-1-P, which is a precursor for the biosynthesis of chitin and for the formation of N-glycosylated mannoproteins and glycosylphosphatidylinositol anchors |
| SDD1 |
YEL057C |
Uncharacterized protein YEL057C; Protein of unknown function; overproduction suppresses lethality due to expression of the dominant PET9 ele AAC2-A128P; may have a role in telomere maintenance; target of UME6 regulation |
| HAT2 |
YEL056W |
Subunit of the Hat1p-Hat2p histone acetyltransferase complex; required for high affinity binding of the complex to free histone H4, thereby enhancing Hat1p activity; similar to human RbAp46 and 48; has a role in telomeric silencing |
| POL5 |
YEL055C |
DNA Polymerase phi; has sequence similarity to the human MybBP1A and weak sequence similarity to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA |
| SNR80 |
YNCE0001C |
Unknown |
| RPL12A |
YEL054C |
Ribosomal 60S subunit protein L12A; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12A has a paralog, RPL12B, that arose from the whole genome duplication |
| MAK10 |
YEL053C |
N-alpha-acetyltransferase, 35 NatC auxiliary subunit; Non-catalytic subunit of the NatC N-terminal acetyltransferase; required for replication of dsRNA virus; expression is glucose-repressible; human NatC ortholog, Naa35, requires co-expression of the human catalytic subunit, Naa30, to functiony complement the null ele; Belongs to the MAK10 family |
| AFG1 |
YEL052W |
Peroxisome-assembly atpase; Protein that may act as a chaperone for cytochrome c oxidase subunits; conserved protein; may act as a chaperone in the degradation of misfolded or unassembled cytochrome c oxidase subunits; localized to matrix face of the mitochondrial inner membrane; member of the AAA family but lacks a protease domain |
| VMA8 |
YEL051W |
Subunit D of the V1 peripheral membrane domain of V-ATPase; part of the electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis; the V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase) has eight subunits |
| RML2 |
YEL050C |
Mitochondrial ribosomal protein of the large subunit (L2); has similarity to E. coli L2 ribosomal protein; mutant ele (fat21) causes inability to utilize oleate, and induce oleic acid oxidation; may interfere with activity of the Adr1p transcription factor |
| SNR67 |
YNCE0002W |
Unknown |
| SNR53 |
YNCE0003W |
Unknown |
| PAU2 |
YEL049W |
Seripauperin-2; Member of the seripauperin multigene family; encoded mainly in subtelomeric region; active during alcoholic fermentation; regulated by anaerobiosis; negatively regulated by oxygen; repressed by heme |
| TCA17 |
YEL048C |
TRAPP-associated protein TCA17; Component of transport protein particle (TRAPP) complex II; TRAPPII is a multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating intra-Golgi and endosome-Golgi traffic; promotes association of TRAPPII-specific subunits with the TRAPP core complex; sedlin related; human Sedlin mutations cause SEDT, a skeletal disorder |
| FRD1 |
YEL047C |
Soluble fumarate reductase; required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to Arxula adeninovorans fumarate reductase; protein abundance increases in response to DNA replication stress; FRD1 has a paralog, OSM1, that arose from the whole genome duplication; Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily |
| GLY1 |
YEL046C |
Low specificity L-threonine aldolase; Threonine aldolase; catalyzes the cleavage of L-o-threonine and L-threonine to glycine; involved in glycine biosynthesis |
| IES6 |
YEL044W |
Chromatin-remodeling complex subunit IES6; Component of the INO80 chromatin remodeling complex; critical for INO80 function; involved in regulation of chromosome segregation and maintenance of normal centromeric chromatin structure; human ortholog INO80C is a member of the human INO80 complex; implicated in DNA repair based on genetic interactions with RAD52 epistasis genes; Belongs to the IES6 family |
| GTA1 |
YEL043W |
Uncharacterized protein YEL043W; Predicted cytoskeleton protein involved in intracellular signaling; based on quantitative analysis of protein-protein interaction maps; may interact with ribosomes, based on co-purification studies; contains fibronectin type III domain fold |
| GDA1 |
YEL042W |
Guanosine-diphosphatase; Guanosine diphosphatase located in the Golgi; involved in the transport of GDP-mannose into the Golgi lumen, converting GDP to GMP after mannose is transferred to substrates; null mutants are defective in sporulation and pre-meiotic S phase entry; orthologous to human ENTPD6, a meiosis-associated non-obstructive azoospermia (NOA) related gene identified in GWAS studies; Belongs to the GDA1/CD39 NTPase family |
| YEF1 |
YEL041W |
ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication |
| UTR2 |
YEL040W |
Probable glycosidase CRH2; Chitin transglycosylase; functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell w; similar to and functiony redundant with Crh1; glycosylphosphatidylinositol (GPI)-anchored protein localized to bud neck |
| CYC7 |
YEL039C |
Cytochrome c isoform 2, expressed under hypoxic conditions; also known as iso-2-cytochrome c; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; protein abundance increases in response to DNA replication stress; CYC7 has a paralog, CYC1, that arose from the whole genome duplication |
| UTR4 |
YEL038W |
Enolase-phosphatase E1; Protein with sequence similarity to acireductone synthases; involved in methionine salvage; found in both the cytoplasm and nucleus; Belongs to the HAD-like hydrolase superfamily. MasA/MtnC family |
| RAD23 |
YEL037C |
UV excision repair protein RAD23; Protein with ubiquitin-like N terminus; subunit of Nuclear Excision Repair Factor 2 (NEF2) with Rad4p that binds damaged DNA; enhances protein deglycosylation activity of Png1p; also involved, with Rad4p, in ubiquitylated protein turnover; Rad4p-Rad23p heterodimer binds to promoters of DNA damage response genes to repress their transcription in the absence of DNA damage |
| ANP1 |
YEL036C |
Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol |
| UTR5 |
YEL035C |
Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions |
| HYP2 |
YEL034W |
Eukaryotic translation initiation factor 5A-1; Translation elongation factor eIF-5A; required for translation of proteins containing polyproline stretches, including Bni1p, and this leads to a requirement for mating projection formation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication; human EIF5A complements the inviability of the yeast hyp2 anb1 double null mutant |
| MTC7 |
YEL033W |
Maintenance of telomere capping protein 7; Protein of unknown function; predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant |
| YNCE0005W |
YNCE0005W |
Unknown |
| MCM3 |
YEL032W |
DNA replication licensing factor MCM3; Protein involved in DNA replication; component of the Mcm2-7 hexameric helicase complex that binds chromatin as a part of the pre-replicative complex |
| SPF1 |
YEL031W |
Manganese-transporting ATPase 1; P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 |
| ECM10 |
YEL030W |
Heat shock protein of the Hsp70 family; localized in mitochondrial nucleoids, plays a role in protein translocation, interacts with Mge1p in an ATP-dependent manner; overexpression induces extensive mitochondrial DNA aggregations; ECM10 has a paralog, SSC1, that arose from the whole genome duplication |
| BUD16 |
YEL029C |
Putative pyridoxal kinase; a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) |
| YEL028W |
YEL028W |
Uncharacterized protein YEL028W; Putative protein of unknown function; conserved among S. cerevisiae strains; YEL028C is not an essential gene |
| IMT4 |
YNCE0006W |
Unknown |
| VMA3 |
YEL027W |
V-type proton ATPase subunit c; Proteolipid subunit c of the V0 domain of vacuolar H(+)-ATPase; dicyclohexylcarbodiimide binding subunit; required for vacuolar acidification and important for copper and iron metal ion homeostasis; Belongs to the V-ATPase proteolipid subunit family |
| SNU13 |
YEL026W |
13 kDa ribonucleoprotein-associated protein; RNA binding protein; part of U3 snoRNP involved in rRNA processing, part of U4/U6-U5 tri-snRNP involved in mRNA splicing, similar to human 15.5K protein; Belongs to the eukaryotic ribosomal protein eL8 family |
| YEL025C |
YEL025C |
Uncharacterized protein YEL025C; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus |
| RIP1 |
YEL024W |
Ubiquinol-cytochrome-c reductase; a Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex; transfers electrons from ubiquinol to cytochrome c1 during respiration; during import, Rip1p is first imported into the mitochondrial matrix where it is processed, acquires its Fe-S cluster, and is folded, then is translocated into the inner membrane by the action of a homo-oligomer of Bcs1p, and finy is delivered by Bcs1p to Complex III for assembly |
| YEL023C |
YEL023C |
Uncharacterized protein YEL023C; Putative protein of unknown function; expression is increased greatly during sporulation; YEL023C is not an essential gene |
| GEA2 |
YEL022W |
Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication |
| URA3 |
YEL021W |
Orotidine-5'-phosphate (OMP) decarboxylase; catalyzes the sixth enzymatic step in the de novo biosynthesis of pyrimidines, converting OMP into uridine monophosphate (UMP); converts 5-FOA into 5-fluorouracil, a toxic compound |
| TIM9 |
YEL020W-A |
Mitochondrial import inner membrane translocase subunit TIM9; Essential protein of the mitochondrial intermembrane space; forms a complex with Tim10p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane |
| RPR1 |
YNCE0007C |
Unknown |
| PXP1 |
YEL020C |
Putative 2-hydroxyacyl-CoA lyase; Peroxisomal matrix protein; well-conserved in fungi; contains tripartite homology domain of thiamine pyrophosphate (TPP) enzymes; targeted to peroxisomes by Pex5p; contains low sequence identity with Pdc1p; mRNA identified as translated by ribosome profiling data |
| MMS21 |
YEL019C |
Highly conserved SUMO E3 ligase subunit of SMC5-SMC6 complex; required for anchoring dsDNA breaks to the nuclear periphery; SMC5-SMC6 plays a key role in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; required for efficient sister chromatid cohesion; mutants are sensitive to MMS, show increased spontaneous mutation and mitotic recombination; SUMOylates and inhibits Snf1p function; supports nucleolar function; Belongs to the NSE2 family |
| EAF5 |
YEL018W |
Chromatin modification-related protein EAF5; Non-essential subunit of the NuA4 acetyltransferase complex; Esa1p-associated factor; relocalizes to the cytosol in response to hypoxia |
| PMP2 |
YEL017C-A |
Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication |
| GTT3 |
YEL017W |
Glutathione transferase 3; Protein of unknown function may be involved in glutathione metabolism; function suggested by computational analysis of large-scale protein-protein interaction data; N- and C-terminal fusion proteins localize to the cell periphery |
| NPP2 |
YEL016C |
Ectonucleotide pyrophosphatase/phosphodiesterase 2; Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp1p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; involved in spore w assembly; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum; NPP2 has a paralog, NPP1, that arose from the whole genome duplication; npp1 npp2 double mutant exhibits reduced dityrosine fluorescence relative to single mutants |
| EDC3 |
YEL015W |
Enhancer of mRNA-decapping protein 3; Non-essential conserved protein with a role in mRNA decapping; specificy affects the function of the decapping enzyme Dcp1p; mediates decay of the RPS28B mRNA via binding to both Rps28Bp (or Rps28Ap) and the RPS28B mRNA; mediates decay of the YRA1 mRNA by a different, translation-independent mechanism; localizes to cytoplasmic mRNA processing bodies; forms cytoplasmic foci upon DNA replication stress |
| YEL014C |
YEL014C |
Uncharacterized protein YEL014C; Putative protein of unknown function; conserved among S. cerevisiae strains |
| VAC8 |
YEL013W |
Vacuolar protein 8; Phosphorylated and palmitoylated vacuolar membrane protein; interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions |
| YNCE0008C |
YNCE0008C |
Unknown |
| UBC8 |
YEL012W |
Ubiquitin-conjugating enzyme that regulates gluconeogenesis; negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro |
| GLC3 |
YEL011W |
Glycogen branching enzyme, involved in glycogen accumulation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress; glycogen accumulation defect of the null mutant is functiony complemented by human GBE1, which is associated with glycogen storage disease; Belongs to the glycosyl hydrolase 13 family. GlgB subfamily |
| YNCE0009C |
YNCE0009C |
Unknown |
| YEL009C-A |
YEL009C-A |
Uncharacterized protein YEL009C-A; Putative protein of unknown function; conserved among S. cerevisiae strains; overlaps ORF YEL010W |
| YNCE0010W |
YNCE0010W |
Unknown |
| GCN4 |
YEL009C |
General control protein GCN4; bZIP transcriptional activator of amino acid biosynthetic genes; activator responds to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels; Belongs to the bZIP family. GCN4 subfamily |
| YEL008W |
YEL008W |
Uncharacterized protein YEL008W; Putative protein of unknown function; conserved among S. cerevisiae strains; YEL008W is not an essential gene; predicted to be involved in metabolism |
| MIT1 |
YEL007W |
Gti1/Pac2 family transcription factor; Transcriptional regulator of pseudohyphal growth; protein with sequence similarity to S. pombe gti1+ (gluconate transport inducer 1) and C. albicans Wor1 |
| YEA6 |
YEL006W |
Mitochondrial nicotinamide adenine dinucleotide transporter 2; Putative mitochondrial NAD+ transporter; member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog; YEA6 has a paralog, YIA6, that arose from the whole genome duplication |
| VAB2 |
YEL005C |
Subunit of the BLOC-1 complex involved in endosomal maturation; interacts with Vps21p-GFP; has potential role in vacuolar function, as suggested by its ability to bind Vac8p; likely member of; Vab2p-GFP-fusion localizes to cytoplasm in punctate pattern |
| YEA4 |
YEL004W |
Solute carrier family 35 (udp-xylose/udp-n-acetylglucosamine transporter), member b4; UDP-N-acetylglucosamine transporter YEA4; Uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) transporter; required for cell w chitin synthesis; localized to the ER |
| GIM4 |
YEL003W |
Prefoldin subunit 2; Subunit of the heterohexameric cochaperone prefoldin complex; complex binds specificy to cytosolic chaperonin and transfers target proteins to it |
| WBP1 |
YEL002C |
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1; Beta subunit of the oligosaccharyl transferase glycoprotein complex; required for N-linked glycosylation of proteins in the endoplasmic reticulum; human homolog DDOST can complement yeast growth defect during down-regulation of yeast gene; Belongs to the DDOST 48 kDa subunit family |
| IRC22 |
YEL001C |
Increased recombination centers protein 22; Protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; YEL001C is non-essential; null mutant displays increased levels of spontaneous Rad52p foci; Belongs to the IRC22 family |
| MNN1 |
YER001W |
Alpha-1,3-mannosyltransferase; integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 family; Belongs to the MNN1/MNT family |
| NOP16 |
YER002W |
Nucleolar protein 16; Constituent of 66S pre-ribosomal particles; involved in 60S ribosomal subunit biogenesis; Belongs to the NOP16 family |
| PMI40 |
YER003C |
Mannose-6-phosphate isomerase; catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation |
| FMP52 |
YER004W |
Protein FMP52, mitochondrial; Protein of unknown function; localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation; Belongs to the FMP52 family |
| YND1 |
YER005W |
Apyrase with wide substrate specificity; helps prevent inhibition of glycosylation by hydrolyzing nucleoside tri- and diphosphates that inhibit glycotransferases; partiy redundant with Gda1p; mediates adenovirus E4orf4-induced toxicity |
| NUG1 |
YER006W |
Nuclear GTP-binding protein NUG1; GTPase that associates with nuclear 60S pre-ribosomes; required for export of 60S ribosomal subunits from the nucleus |
| PAC2 |
YER007W |
Protein PAC2; Microtubule effector required for tubulin heterodimer formation; binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl |
| TMA20 |
YER007C-A |
Translation machinery-associated protein 20; Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; interacts with Tma22p; null mutant exhibits translation defects; has homology to human oncogene MCT-1; protein abundance increases in response to DNA replication stress; Belongs to the TMA20 family |
| SNR14 |
YNCE0011C |
Unknown |
| SEC3 |
YER008C |
Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress; Belongs to the SEC3 family |
| NTF2 |
YER009W |
Nuclear transport factor 2; Nuclear envelope protein; interacts with GDP-bound Gsp1p and with proteins of the nuclear pore to transport Gsp1p into the nucleus where it is an essential player in nucleocytoplasmic transport |
| YER010C |
YER010C |
Bifunctional HMG aldolase/oxaloacetate decarboxylase; requires divalent metal ions for activity; competitively inhibited by oxalate; forms a ring-shaped homotrimer; similar to members of the prokaryotic RraA family of class II (divalent metal ion dependent) pyruvate aldolases from the meta cleavage pathways of protocatechuate and gate |
| TIR1 |
YER011W |
Cold shock-induced protein TIR1; Cell w mannoprotein; expression is downregulated at acidic pH and induced by cold shock and anaerobiosis; abundance is increased in cells cultured without shaking; member of the Srp1p/Tip1p family of serine-alanine-rich proteins; Belongs to the SRP1/TIP1 family |
| YNCE0012W |
YNCE0012W |
Unknown |
| PRE1 |
YER012W |
Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle; Belongs to the peptidase T1B family |
| PRP22 |
YER013W |
DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase; associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes; required for proofreading the exon ligation reaction |
| HEM14 |
YER014W |
Protoporphyrinogen oxidase; a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides |
| BUD25 |
YER014C-A |
Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern |
| FAA2 |
YER015W |
Long-chain-fatty-acid--CoA ligase 2; Medium chain fatty acyl-CoA synthetase; activates imported fatty acids; accepts a wide range of fatty acid chain lengths with a preference for medium chains, C9:0-C13:0; localized to the peroxisome; comparative analysis suggests that a mitochondriy targeted form may result from translation starting at a non-canonical codon upstream of the annotated start codon |
| BIM1 |
YER016W |
Microtubule-associated protein, rp/eb family; Protein BIM1; Microtubule plus end-tracking protein; together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormy; homolog of human end binding protein 1 (EB1); Belongs to the MAPRE family |
| AFG3 |
YER017C |
Mitochondrial respiratory chain complexes assembly protein AFG3; Mitochondrial inner membrane m-AAA protease component; mediates degradation of misfolded or unassembled proteins; also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging; expression of human homolog AFG3L2 can complement yeast yta12 afg3 double mutant; In the N-terminal section; belongs to the AAA ATPase family |
| SPC25 |
YER018C |
Kinetochore protein SPC25; Component of the kinetochore-associated Ndc80 complex; involved in chromosome segregation, spindle checkpoint activity, and kinetochore clustering; evolutionarily conserved; other members include Ndc80p, Nuf2p, Spc24p, and Spc25p |
| ISC1 |
YER019W |
Inositol phosphosphingolipid phospholipase C; mitochondrial membrane localized; hydrolyzes complex sphingolipids to produce ceramide; activates genes required for non-fermentable carbon source metabolism during diauxic shift; activated by phosphatidylserine, cardiolipin, and phosphatidylglycerol; mediates Na+ and Li+ halotolerance; ortholog of mammalian neutral sphingomyelinase type 2 |
| SBH2 |
YER019C-A |
Arf family guanine nucleotide exchange factor sbh2; Ssh1p-Sss1p-Sbh2p complex component; involved in protein translocation into the endoplasmic reticulum; SBH2 has a paralog, SBH1, that arose from the whole genome duplication; Belongs to the SEC61-beta family |
| GPA2 |
YER020W |
Guanine nucleotide-binding protein alpha-2 subunit; Nucleotide binding alpha subunit of the heterotrimeric G protein; interacts with the receptor Gpr1p, has signaling role in response to nutrients; required for the recruitment of Ras-GTP at the plasma membrane and in the nucleus |
| RPN3 |
YER021W |
Essential non-ATPase regulatory subunit of the 26S proteasome lid; similar to the p58 subunit of the human 26S proteasome; temperature-sensitive eles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control |
| SRB4 |
YER022W |
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for basal RNA polymerase II transcription; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination |
| PRO3 |
YER023W |
Delta 1-pyrroline-5-carboxylate reductase; catalyzes the last step in proline biosynthesis |
| YAT2 |
YER024W |
Carnitine O-acetyltransferase YAT2; Carnitine acetyltransferase; has similarity to Yat1p, which is a carnitine acetyltransferase associated with the mitochondrial outer membrane |
| GCD11 |
YER025W |
Gamma subunit of the translation initiation factor eIF2; involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met; mutations in human ortholog cause X-linked intellectual disability (XLID); Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EIF2G subfamily |
| YNCE0013W |
YNCE0013W |
Unknown |
| CHO1 |
YER026C |
CDP-diacylglycerol--serine O-phosphatidyltransferase; Phosphatidylserine synthase; functions in phospholipid biosynthesis; catalyzes the reaction CDP-diaclyglycerol + L-serine = CMP + L-1-phosphatidylserine, transcriptiony repressed by myo-inositol and choline |
| GAL83 |
YER027C |
One of three possible beta-subunits of the Snf1 kinase complex; ows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; necessary and sufficient for phosphorylation of the Mig2p transcription factor in response to alkaline stress; functiony redundant with SIP1 and SIP2 for the phosphorylation of Mig1p in response to glucose deprivation; contains a glycogen-binding domain |
| MIG3 |
YER028C |
Transcription corepressor MIG3; Transcriptional regulator; partiy nonfunctional in S288C strains but has a major role in catabolite repression and ethanol response in some other strains; involved in response to toxic agents; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, owing induction of damage response genesenvironment; Belongs to the creA/MIG C2H2-type zinc-finger protein family |
| SMB1 |
YER029C |
Sm nuclear ribonucleoprotein-associated protein B; Core Sm protein Sm B; part of heteroheptameric complex (with Smd1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm B and Sm B'; Belongs to the snRNP SmB/SmN family |
| CHZ1 |
YER030W |
Histone chaperone for Htz1p/H2A-H2B dimer; required for the stabilization of the Chz1p-Htz1-H2B complex; has overlapping function with Nap1p; null mutant displays weak sensitivity to MMS and benomyl; contains a highly conserved CHZ motif; protein abundance increases in response to DNA replication stress |
| YPT31 |
YER031C |
GTP-binding protein YPT31/YPT8; Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication; localizes to the transitional and late Golgi |
| FIR1 |
YER032W |
Factor interacting with REF2; Protein involved in 3' mRNA processing; interacts with Ref2p; APCC(Cdh1) substrate; potential Cdc28p substrate |
| ZRG8 |
YER033C |
Zinc-regulated protein 8; Protein of unknown function; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; GFP-fusion protein is localized to the cytoplasm; transcription induced under conditions of zinc deficiency |
| YER034W |
YER034W |
Uncharacterized protein YER034W; Protein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress |
| EDC2 |
YER035W |
Enhancer of mRNA-decapping protein 2; RNA-binding protein that directly activates mRNA decapping; binds mRNA substrate and enhances activity of decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein increases in abundance and relocalizes to nucleolus and to nuclear foci upon DNA replication stress; EDC2 has a paralog, EDC1, that arose from the whole genome duplication |
| ARB1 |
YER036C |
ABC transporter ATP-binding protein ARB1; ATPase of the ATP-binding cassette (ABC) family; involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; shuttles from nucleus to cytoplasm, physicy interacts with Tif6p, Lsg1p; human homolog ABCF2 can complement yeast ARB1 mutant; Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily |
| PHM8 |
YER037W |
Phosphate metabolism protein 8; Lysophosphatidic acid (LPA) phosphatase, nucleotidase; principle and physiological nucleotidase working on GMP, UMP and CMP; involved in LPA hydrolysis in response to phosphate starvation and ribose salvage pathway; phosphatase activity is soluble and Mg2+ dependent; expression is induced by low phosphate levels and by inactivation of Pho85p; repressed by Gcn4p under normal conditions; PHM8 has a paralog, SDT1, that arose from the whole genome duplication |
| KRE29 |
YER038C |
DNA repair protein KRE29; Subunit of the SMC5-SMC6 complex; this complex is involved in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance |
| FMP49 |
YER038W-A |
Fmp49p; Mitochondrial protein of unknown function; almost completely overlaps ORF HVG1/YER039C |
| HVG1 |
YER039C |
Probable GDP-mannose transporter 2; Protein of unknown function; HVG1 has a paralog, VRG4, that arose from the whole genome duplication |
| YER039C-A |
YER039C-A |
Putative protein of unknown function; YER039C-A is not an essential gene; Belongs to the TPT transporter family. SLC35D subfamily |
| GLN3 |
YER040W |
Nitrogen regulatory protein GLN3; Transcriptional activator of genes regulated by nitrogen catabolite repression; localization and activity regulated by quality of nitrogen source and Ure2p |
| YEN1 |
YER041W |
Holliday junction resolvase; promotes template switching during break-induced replication (BIR), causing non-reciprocal translocations (NRTs); localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1; similar to S. cerevisiae endonuclease Rth1p |
| MXR1 |
YER042W |
Methionine-S-sulfoxide reductase; involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR2; involved in the regulation of lifespan; reduced activity of human homolog implicated in Alzheimer disease |
| SAH1 |
YER043C |
Adenosylhomocysteinase; S-adenosyl-L-homocysteine hydrolase; catabolizes S-adenosyl-L-homocysteine which is formed after donation of the activated methyl group of S-adenosyl-L-methionine (AdoMet) to an acceptor; regulates cellular lipid homoeostasis by regulating phosphatidylcholine(PC)synthesis and triacylglycerol (TG) levels |
| ERG28 |
YER044C |
Ergosterol biosynthetic protein 28; Endoplasmic reticulum membrane protein; may facilitate protein-protein interactions between the Erg26p dehydrogenase and the Erg27p 3-ketoreductase and/or tether these enzymes to the ER, also interacts with Erg6p |
| MEI4 |
YER044C-A |
Meiosis-specific protein involved in forming DSBs; involved in double-strand break (DSBs) formation during meiotic recombination; required for chromosome synapsis and production of viable spores |
| ACA1 |
YER045C |
ATF/CREB activator 1; ATF/CREB family basic leucine zipper (bZIP) transcription factor; binds as a homodimer to the ATF/CREB consensus sequence TGACGTCA; important for carbon source utilization; target genes include GRE2 and COS8; ACA1 has a paralog, CST6, that arose from the whole genome duplication |
| SPO73 |
YER046W |
Sporulation-specific protein 73; Meiosis-specific protein required for prospore membrane morphogenesis; required for the proper shape of the prospore membrane (PSM) and for spore w formation; functions cooperatively with SPO71 in PSM elongation; physicy interacts with Spo71p; geneticy antagonistic to SPO1, similar to SPO71; localizes to the PSM; required for spore w formation during sporulation; dispensable for both nuclear divisions during meiosis; dysferlin domain-only protein |
| SAP1 |
YER047C |
Protein SAP1; Putative ATPase of the AAA family; interacts with the Sin1p transcriptional repressor in the two-hybrid system |
| CAJ1 |
YER048C |
Protein CAJ1; Nuclear type II J heat shock protein of the E. coli dnaJ family; contains a leucine zipper-like motif, binds to non-native substrates for presentation to Ssa3p, may function during protein translocation, assembly and disassembly |
| YNCE0014W |
YNCE0014W |
Unknown |
| ISD11 |
YER048W-A |
Protein ISD11; Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis; exclusive to eukaryotes, implicated as eukaryotic supplement to the bacterium-derived Fe-S cluster (ISC) assembly apparatus; involved in regulation of iron metabolism; member of the LYR protein family |
| TPA1 |
YER049W |
Prolyl 3,4-dihydroxylase TPA1; Fe(II)/2-oxoglutarate-dependent dioxygenase family member; catalyzes the repair of methyl-base lesions in both ss and dsDNA by oxidative demethylation; Poly(rA)-binding protein involved in mRNA poly(A) tail length and mRNA stability; role in translation termination efficiency; interacts with Sup45p (eRF1), Sup35p (eRF3) and Pab1p; similar to human prolyl 4-hydroxylase OGFOD1; binds Fe(II) and 2-oxoglutarate |
| RSM18 |
YER050C |
37S ribosomal protein RSM18, mitochondrial; Mitochondrial ribosomal protein of the sm subunit; has similarity to E. coli S18 ribosomal protein |
| JHD1 |
YER051W |
JmjC domain family histone demethylase specific for H3-K36; similar to proteins found in human, mouse, drosophila, X. laevis, C. elegans, and S. pombe; Belongs to the JHDM1 histone demethylase family |
| HOM3 |
YER052C |
Aspartokinase; Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis; Belongs to the aspartokinase family |
| PIC2 |
YER053C |
Solute carrier family 25 (mitochondrial phosphate transporter), member 3; Mitochondrial copper and phosphate carrier; imports copper and inorganic phosphate into mitochondria; functiony redundant with Mir1p but less abundant than Mir1p under normal conditions; expression is induced at high temperature |
| YER053C-A |
YER053C-A |
Uncharacterized protein YER053C-A; Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress |
| GIP2 |
YER054C |
GLC7-interacting protein 2; Putative regulatory subunit of protein phosphatase Glc7p; involved in glycogen metabolism; contains a conserved motif (GVNK motif) that is also found in Gac1p, Pig1p, and Pig2p; GIP2 has a paralog, PIG2, that arose from the whole genome duplication |
| HIS1 |
YER055C |
ATP phosphoribosyltransferase; a hexameric enzyme, catalyzes the first step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control |
| FCY2 |
YER056C |
Purine-cytosine permease; mediates purine (adenine, guanine, and hypoxanthine) and cytosine accumulation; relative distribution to the vacuole increases upon DNA replication stress |
| RPL34A |
YER056C-A |
Ribosomal 60S subunit protein L34A; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34A has a paralog, RPL34B, that arose from the whole genome duplication |
| HMF1 |
YER057C |
Protein HMF1; Member of the p14.5 protein family; functiony complements Mmf1p function when targeted to mitochondria; heat shock inducible; high-dosage growth inhibitor; forms a homotrimer in vitro; HMF1 has a paralog, MMF1, that arose from the whole genome duplication; Belongs to the RutC family |
| PET117 |
YER058W |
Protein PET117, mitochondrial; Protein required for assembly of cytochrome c oxidase |
| PCL6 |
YER059W |
Pho85p cyclin of the Pho80p subfamily; forms the major Glc8p kinase together with Pcl7p and Pho85p; involved in the control of glycogen storage by Pho85p; stabilized by Elongin C binding; PCL6 has a paralog, PCL7, that arose from the whole genome duplication |
| FCY21 |
YER060W |
Purine-cytosine permease fcy21; Putative purine-cytosine permease; very similar to Fcy2p but cannot substitute for its function |
| FCY22 |
YER060W-A |
Purine-cytosine permease fcy22; Putative purine-cytosine permease; very similar to Fcy2p but cannot substitute for its function |
| CEM1 |
YER061C |
3-oxoacyl-[acyl-carrier-protein] synthase homolog; Mitochondrial beta-keto-acyl synthase; possible role in fatty acid synthesis; required for mitochondrial respiration; human homolog OXSM can complement yeast cem1 null mutant; Belongs to the beta-ketoacyl-ACP synthases family |
| GPP2 |
YER062C |
Glycerol-1-phosphate phosphohydrolase 2; DL-glycerol-3-phosphate phosphatase involved in glycerol biosynthesis; also known as glycerol-1-phosphatase; induced in response to hyperosmotic or oxidative stress, and during diauxic shift; GPP2 has a paralog, GPP1, that arose from the whole genome duplication; Belongs to the HAD-like hydrolase superfamily. DOG/GPP family |
| THO1 |
YER063W |
Sap domain-containing ribonucleoprotein; Protein THO1; Conserved nuclear RNA-binding protein; specificy binds to transcribed chromatin in a THO- and RNA-dependent manner, geneticy interacts with shuttling hnRNP NAB2; overproduction suppresses transcriptional defect caused by hpr1 mutation |
| VHR2 |
YER064C |
Transcription factor VHR2; Non-essential nuclear protein; null mutation has global effects on transcription; VHR2 has a paralog, VHR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress |
| ICL1 |
YER065C |
Isocitrate lyase; catalyzes the formation of succinate and glyoxylate from isocitrate, a key reaction of the glyoxylate cycle; expression of ICL1 is induced by growth on ethanol and repressed by growth on glucose |
| SUP19 |
YNCE0015C |
Unknown |
| RRT13 |
YER066W |
Regulator of rDNA transcription protein 13; Putative protein of unknown function; non-essential gene identified in a screen for mutants with decreased levels of rDNA transcription |
| RGI1 |
YER067W |
Respiratory growth induced protein 1; Protein of unknown function; involved in energy metabolism under respiratory conditions; protein abundance is increased upon intracellular iron depletion or in response to DNA replication stress; RGI1 has a paralog, RGI2, that arose from the whole genome duplication |
| MOT2 |
YER068W |
General negative regulator of transcription subunit 4; Ubiquitin-protein ligase subunit of the CCR4-NOT complex; with Ubc4p, ubiquitinates nascent polypeptide-associated complex subunits and histone demethyase Jhd2p; CCR4-NOT has roles in transcription regulation, mRNA degradation, and post-transcriptional modifications; regulates levels of DNA Polymerase-{alpha} to promote efficient and accurate DNA replication |
| ARG5,6 |
YER069W |
Protein ARG5,6, mitochondrial; Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine |
| RNR1 |
YER070W |
Ribonucleoside-diphosphate reductase large chain 1; Major isoform of large subunit of ribonucleotide-diphosphate reductase; the RNR complex catalyzes rate-limiting step in dNTP synthesis, regulated by DNA replication and DNA damage checkpoint pathways via localization of sm subunits; relative distribution to the nucleus increases upon DNA replication stress; RNR1 has a paralog, RNR3, that arose from the whole genome duplication |
| TDA2 |
YER071C |
Topoisomerase I damage affected protein 2; Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; null mutant is sensitive to expression of the top1-T722A ele |
| VTC1 |
YER072W |
Regulatory subunit of the vacuolar transporter chaperone (VTC) complex; VTC complex is involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion; also has mRNA binding activity; protein abundance increases in response to DNA replication stress |
| ALD5 |
YER073W |
Mitochondrial aldehyde dehydrogenase; involved in regulation or biosynthesis of electron transport chain components and acetate formation; activated by K+; utilizes NADP+ as the preferred coenzyme; constitutively expressed |
| RPS24A |
YER074W |
Protein component of the sm (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication |
| YOS1 |
YER074W-A |
Integral membrane protein required for ER to Golgi transport; localized to the Golgi, the ER, and COPII vesicles; interacts with Yip1p and Yif1p |
| PTP3 |
YER075C |
Tyrosine-protein phosphatase 3; Phosphotyrosine-specific protein phosphatase; involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the cytoplasm |
| YNCE0016C |
YNCE0016C |
Unknown |
| YER076C |
YER076C |
Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization; To yeast killer toxin KHR |
| MRX1 |
YER077C |
MIOREX complex component 1; Protein that associates with mitochondrial ribosome; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport |
| ICP55 |
YER078C |
Intermediate cleaving peptidase 55; Mitochondrial aminopeptidase; cleaves the N termini of at least 38 imported proteins after cleavage by the mitochondrial processing peptidase (MPP), thereby increasing their stability; member of the aminopeptidase P family |
| YER078W-A |
YER078W-A |
Uncharacterized protein YER078W-A; Putative protein of unknown function; identified by fungal homology and RT-PCR |
| YER079W |
YER079W |
Uncharacterized protein YER079W; Putative protein of unknown function |
| AIM9 |
YER080W |
Altered inheritance of mitochondria protein 9, mitochondrial; Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss |
| SRG1 |
YNCE0017W |
Unknown |
| SER3 |
YER081W |
D-3-phosphoglycerate dehydrogenase 1; 3-phosphoglycerate dehydrogenase and alpha-ketoglutarate reductase; 3PG dehydrogenase that catalyzes the first step in serine and glycine biosynthesis; also functions as an alpha-ketoglutarate reductase, converting alpha-ketoglutarate to D-2-hydroxyglutarate (D-2HG); localizes to the cytoplasm; SER3 has a paralog, SER33, that arose from the whole genome duplication |
| UTP7 |
YER082C |
U3 sm nucleolar RNA-associated protein 7; Nucleolar protein; component of the sm subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA |
| GET2 |
YER083C |
Golgi to ER traffic protein 2; Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division; Belongs to the GET2 family |
| YER084W |
YER084W |
Uncharacterized protein YER084W; Protein of unknown function; expressed at both mRNA and protein levels |
| YER085C |
YER085C |
Uncharacterized protein YER085C; Putative protein of unknown function |
| ILV1 |
YER086W |
Threonine dehydratase, mitochondrial; Threonine deaminase, catalyzes first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation; Belongs to the serine/threonine dehydratase family |
| AIM10 |
YER087W |
Probable proline--tRNA ligase, mitochondrial; Protein with similarity to tRNA synthetases; non-tagged protein is detected in purified mitochondria; null mutant is viable and displays elevated frequency of mitochondrial genome loss |
| SBH1 |
YER087C-B |
Arf family guanine nucleotide exchange factor sbh1; Protein transport protein SBH1; Beta subunit of Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; cotranslationy N-acetylated by NatA; SBH1 has a paralog, SBH2, that arose from the whole genome duplication; Belongs to the SEC61-beta family |
| DOT6 |
YER088C |
Transcriptional regulatory protein DOT6; Protein involved in rRNA and ribosome biogenesis; activated in stochastic pulses of nuclear localization; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; relative distribution to the nucleus increases upon DNA replication stress; Belongs to the DOT6 family |
| PTC2 |
YER089C |
Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p to limit maximal osmostress induced kinase activity; dephosphorylates Ire1p to downregulate the unfolded protein response; dephosphorylates Cdc28p; inactivates the DNA damage checkpoint; PTC2 has a paralog, PTC3, that arose from the whole genome duplication |
| TRP2 |
YER090W |
Anthranilate synthase component i; Anthranilate synthase; catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p |
| MET6 |
YER091C |
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase; Cobalamin-independent methionine synthase; involved in methionine biosynthesis and regeneration; requires a minimum of two glutamates on the methyltetrahydrofolate substrate, similar to bacterial metE homologs |
| IES5 |
YER092W |
Ino eighty subunit 5; Non-essential INO80 chromatin remodeling complex subunit; deletion affects telomere maintenance via recombination |
| TSC11 |
YER093C |
Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p); TORC2 is a membrane-associated complex that regulates actin cytoskeletal dynamics during polarized growth and cell w integrity; involved in sphingolipid metabolism; contains a RasGEFN domain; Belongs to the RICTOR family |
| AIM11 |
YER093C-A |
Protein of unknown function; null mutant is viable but shows increased loss of mitochondrial genome and synthetic interaction with prohibitin (phb1); contains an intron; SWAT-GFP and mCherry fusion proteins localize to the mitochondria; YER093C-A has a paralog, YBL059W, that arose from the whole genome duplication |
| PUP3 |
YER094C |
Beta 3 subunit of the 20S proteasome; involved in ubiquitin-dependent catabolism; human homolog is subunit C10 |
| RAD51 |
YER095W |
DNA repair protein RAD51; Strand exchange protein; forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein |
| SHC1 |
YER096W |
Protein SHC1; Sporulation-specific activator of Chs3p (chitin synthase III); required for the synthesis of the chitosan layer of ascospores; transcriptiony induced at alkaline pH; SHC1 has a paralog, SKT5, that arose from the whole genome duplication |
| YNCE0018W |
YNCE0018W |
Unknown |
| UBP9 |
YER098W |
Ubiquitin carboxyl-terminal hydrolase 9/13; Ubiquitin-specific protease that cleaves ubiquitin-protein fusions; UBP9 has a paralog, UBP13, that arose from the whole genome duplication; Belongs to the peptidase C19 family |
| PRS2 |
YER099C |
Ribose-phosphate pyrophosphokinase 2; 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP; which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS2 has a paralog, PRS4, that arose from the whole genome duplication |
| UBC6 |
YER100W |
Ubiquitin-conjugating enzyme involved in ERAD; located at the cytosolic side of the ER membrane; tail region contains a transmembrane segment at the C-terminus; substrate of the ubiquitin-proteasome pathway; ER-associated protein degradation is also known as ERAD |
| AST2 |
YER101C |
Protein AST2; Lipid raft associated protein; overexpression restores Pma1p localization to lipid rafts which is required for targeting of Pma1p to the plasma membrane; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST2 has a paralog, AST1, that arose from the whole genome duplication |
| RPS8B |
YER102W |
Protein component of the sm (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8B has a paralog, RPS8A, that arose from the whole genome duplication |
| SSA4 |
YER103W |
Heat shock protein that is highly induced upon stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the HSP70 family; cytoplasmic protein that concentrates in nuclei upon starvation; SSA4 has a paralog, SSA3, that arose from the whole genome duplication |
| RTT105 |
YER104W |
Regulator of ty1 transposition protein 105; Protein with a role in regulation of Ty1 transposition |
| NUP157 |
YER105C |
Nucleoporin NUP157; Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and tethering of DNA to the nuclear periphery; both Nup170p and NUP157p are similar to human Nup155p; NUP157 has a paralog, NUP170, that arose from the whole genome duplication; Belongs to the non-repetitive/WGA-negative nucleoporin family |
| MAM1 |
YER106W |
Monopolin complex subunit MAM1; Monopolin; meiosis-specific kinetochore-associated protein involved in monopolar attachment of sister kinetochores to the meiotic spindle; subunit of monopolin, a complex that prevents biorientation of sister kinetochores to ensure homolog biorientation during meiosis I; regulates the conformation, enzyme kinetics and substrate specificity of the Dsn1p kinase, Hrr1p; expressed only during the first meiotic division |
| GLE2 |
YER107C |
Nucleoporin GLE2; RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 |
| KAP123 |
YER110C |
Importin subunit beta-4; Karyopherin beta; mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1 |
| SWI4 |
YER111C |
Regulatory protein SWI4; DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; Slt2p-independent regulator of cold growth; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p |
| LSM4 |
YER112W |
U6 snRNA-associated Sm-like protein LSm4; Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress |
| TMN3 |
YER113C |
Transmembrane 9 superfamily member 3; Protein with a role in cellular adhesion and filamentous growth; similar to Emp70p and Tmn2p; member of Transmembrane Nine family with 9 transmembrane segments; localizes to Golgi; induced by 8-methoxypsoralen plus UVA irradiation |
| BOI2 |
YER114C |
Protein implicated in polar growth, functiony redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; BOI2 has a paralog, BOI1, that arose from the whole genome duplication |
| SPR6 |
YER115C |
Protein of unknown function; expressed during sporulation; not required for sporulation, but gene exhibits genetic interactions with other genes required for sporulation |
| SLX8 |
YER116C |
Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; role in proteolysis of spindle positioning protein Kar9, DNA repair proteins Rad52p and Rad57p; stimulated by SUMO-modified substrates; contains a C-terminal RING domain; forms nuclear foci upon DNA replication stress; required for maintenance of genome integrity like human ortholog RNF |
| RPL23B |
YER117W |
Ribosomal 60S subunit protein L23B; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23B has a paralog, RPL23A, that arose from the whole genome duplication |
| SHO1 |
YER118C |
High osmolarity signaling protein SHO1; Transmembrane osmosensor for filamentous growth and HOG pathways; involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol (HOG) response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p |
| AVT6 |
YER119C |
Vacuolar amino acid transporter 6; Vacuolar aspartate and glutamate exporter; member of a family of seven genes (AVT1-7) related to vesicular GABA-glycine transporters; involved in compartmentalizing acidic amino acids in response to nitrogen starvation; AVT6 has a paralog, AVT5, that arose from the whole genome duplication |
| SCS2 |
YER120W |
Integral ER membrane protein, regulates phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to plasma membrane (PM) and regulate PI4P levels by controlling access of Sac1p phosphatase to substrate PI4P in the PM; interacts with FFAT motifs in Opi1p, Swh1p, Osh2p, and Osh3p; involved in telomeric silencing; VAP homolog; SCS2 has a paralog, SCS22, that arose from the whole genome duplication; Belongs to the VAMP-associated protein (VAP) (TC 9.B.17) family |
| YER121W |
YER121W |
Uncharacterized protein YER121W; Putative protein of unknown function; may be involved in phosphatase regulation and/or generation of precursor metabolites and energy |
| GLO3 |
YER122C |
ADP-ribosylation factor GTPase activating protein (ARF GAP); involved in ER-Golgi transport; shares functional similarity with Gcs1p |
| YCK3 |
YER123W |
Casein kinase I homolog 3; Palmitoylated vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway |
| DSE1 |
YER124C |
Protein DSE1; Daughter cell-specific protein; may regulate cross-talk between the mating and filamentation pathways; deletion affects cell separation after division and sensitivity to alpha-factor and drugs affecting the cell w; relocalizes from bud neck to cytoplasm upon DNA replication stress; Belongs to the WD repeat DSE1 family |
| RSP5 |
YER125W |
NEDD4 family E3 ubiquitin ligase; regulates processes including: MVB sorting, the heat shock response, transcription, endocytosis and ribosome stability; ubiquitinates Sec23p, Sna3p, Ste4p, Nfi1p, Rpo21p and Sem1p; autoubiquitinates; deubiquitinated by Ubp2p; regulated by SUMO ligase Siz1p, in turn regulates Siz1p SUMO ligase activity; required for efficient Golgi-to-ER trafficking in COPI mutants; mutant tolerates aneuploidy; human homolog implicated in Liddle syndrome; Belongs to the RSP5/NEDD4 family |
| NSA2 |
YER126C |
Ribosome biogenesis protein NSA2; Protein constituent of 66S pre-ribosomal particles; contributes to processing of the 27S pre-rRNA; recruited by ribosomal proteins L17, L35, and L37 to assembling ribosomes after 27SB pre-rRNA is generated, immediately preceding removal of ITS2 |
| LCP5 |
YER127W |
U3 sm nucleolar ribonucleoprotein protein LCP5; Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus |
| VFA1 |
YER128W |
VPS4-associated protein 1; Protein that interacts with Vps4p and has a role in vacuolar sorting; stimulates the ATPase activity of Vps4; localizes to endosomes in a Vps4-dependent manner; overexpression causes canavanine sensitivity and confers a partial class D vacuole morphology |
| SAK1 |
YER129W |
Upstream serine/threonine kinase for the SNF1 complex; plays a role in pseudohyphal groth; partiy redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome; SAK1 has a paralog, TOS3, that arose from the whole genome duplication; Belongs to the protein kinase superfamily. Ser/Thr protein kinase family |
| COM2 |
YER130C |
Zinc finger protein YER130C; Transcription factor that binds IME1 Upstream Activation Signal (UAS)ru; COM2 transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; may bind the IME1 promoter under growth conditions to negatively regulate its transcription in the absence of a positive regulator that binds more effectively; repressor activity may depend on phosphorylation by PKA; C. albicans homolog (MNL1) plays a role in adaptation to stress |
| RPS26B |
YER131W |
Protein component of the sm (40S) ribosomal subunit; homologous to mammalian ribosomal protein S26, no bacterial homolog; RPS26B has a paralog, RPS26A, that arose from the whole genome duplication; human homolog can partiy complement an RPS26A, RPS26B double null mutant; mutations in the human gene are associated with Diamond-Blackfan anemia |
| SNR4 |
YNCE0019W |
Unknown |
| PMD1 |
YER132C |
Protein with an N-terminal kelch-like domain; putative negative regulator of early meiotic gene expression; required, with Mds3p, for growth under alkaline conditions; PMD1 has a paralog, MDS3, that arose from the whole genome duplication |
| SNR52 |
YNCE0020C |
Unknown |
| GLC7 |
YER133W |
Serine/threonine-protein phosphatase PP1-2; Type 1 S/T protein phosphatase (PP1) catalytic subunit; involved in glycogen metabolism, sporulation and mitotic progression; interacts with multiple regulatory subunits; regulates actomyosin ring formation; subunit of CPF; recruited to mating projections by Afr1p interaction; regulates nucleocytoplasmic shuttling of Hxk2p; import into the nucleus is inhibited during spindle assembly checkpoint arrest; involved in dephosphorylating Rps6a/b and Bnr1p; Belongs to the PPP phosphatase family. PP-1 subfamily |
| YNCE0021C |
YNCE0021C |
Unknown |
| YNCE0022C |
YNCE0022C |
Unknown |
| YER134C |
YER134C |
Magnesium-dependent acid phosphatase; member of the haloacid dehalogenase superfamily; non-essential gene; Belongs to the HAD-like hydrolase superfamily |
| YER135C |
YER135C |
Uncharacterized protein YER135C; Putative protein of unknown function; conserved among S. cerevisiae strains; YER135C is not an essential gene |
| YNCE0023W |
YNCE0023W |
Unknown |
| GDI1 |
YER136W |
Rab GDP-dissociation inhibitor; GDP dissociation inhibitor; regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins |
| YER137C |
YER137C |
Uncharacterized protein YEL137C; Putative protein of unknown function |
| SCR1 |
YNCE0024W |
Unknown |
| YNCE0025C |
YNCE0025C |
Unknown |
| RTR1 |
YER139C |
RNA polymerase II subunit B1 CTD phosphatase RTR1; CTD phosphatase; dephosphorylates S5-P in the C-terminal domain of Rpo21p; has a cysteine-rich motif required for function and conserved in eukaryotes; shuttles between the nucleus and cytoplasm; RTR1 has a paralog, RTR2, that arose from the whole genome duplication |
| EMP65 |
YER140W |
Endoplasmic reticulum membrane protein 65; Integral membrane protein of the ER; forms an ER-membrane associated protein complex with Slp1p; identified along with SLP1 in a screen for mutants defective in the unfolded protein response (UPR); proposed to function in the folding of integral membrane proteins; interacts geneticy with MPS3; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; Belongs to the TAPT1 family |
| COX15 |
YER141W |
Cytochrome c oxidase assembly protein COX15; Protein required for the hydroxylation of heme O to form heme A; heme A is an essential prosthetic group for cytochrome c oxidase |
| MAG1 |
YER142C |
3-methyl-adenine DNA glycosylase; involved in protecting DNA against alkylating agents; initiates base excision repair by removing damaged bases to create abasic sites that are subsequently repaired; protein abundance increases in response to DNA replication stress; Belongs to the alkylbase DNA glycosidase AlkA family |
| DDI1 |
YER143W |
DNA damage-inducible v-SNARE binding protein; role in suppression of protein secretion; may play a role in S-phase checkpoint control; has ubiquitin-associated (UBA), ubiquitin-like (UBL), and retroviral-like proteinase (RVP) domains |
| UBP5 |
YER144C |
Putative ubiquitin-specific protease; concentrates at the bud neck; UBP5 has a paralog, DOA4, that arose from the whole genome duplication; Belongs to the peptidase C19 family |
| FTR1 |
YER145C |
High affinity iron permease; involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; protein abundance increases in response to DNA replication stress; Belongs to the oxidase-dependent Fe transporter (OFeT) (TC 9.A.10.1) family |
| YER145C-A |
YER145C-A |
Uncharacterized protein YER145C-A; Putative protein of unknown function; conserved among S. cerevisiae strains; overlaps verified ORF LSM5/YER146W |
| LSM5 |
YER146W |
U6 snRNA-associated Sm-like protein LSm5; Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA |
| SCC4 |
YER147C |
MAU2 chromatid cohesion factor homolog; Subunit of cohesin loading factor (Scc2p-Scc4p); complex is required for the loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during double-strand break repair via phosphorylated histone H2AX |
| SPT15 |
YER148W |
TATA-binding protein (TBP); general transcription factor that interacts with other factors to form the preinitiation complex at promoters; essential for viability, highly conserved; yeast gene can complement mutations in human homolog TBP |
| PEA2 |
YER149C |
Protein PEA2; Coiled-coil 12S polarisome subunit; required for polarity establishment, apical bud growth, shmoo formation, filamentous differentiation; involved in Bni1p localization at sites of polarized growth, controlling polarized assembly of actin cables; role in apical growth affects diploid-specific bipolar bud site selection; retains Slt2p at bud tip to regulate ER inheritance; role in Ca2+ influx, cell fusion; S288C ele encoding Leu409 rather than Met linked with non-invasion |
| SPI1 |
YER150W |
GPI-anchored cell w protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; SPI1 has a paralog, SED1, that arose from the whole genome duplication; Belongs to the SED1 family |
| YNCE0026W |
YNCE0026W |
Unknown |
| UBP3 |
YER151C |
Ubiquitin carboxyl-terminal hydrolase 3; Ubiquitin-specific protease involved in transport and osmotic response; negatively regulates Ras/PKA signaling; interacts with Bre5p to coregulate anterograde, retrograde transport between ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; role in ribophagy; cleaves ubiquitin fusions but not polyubiquitin; protein abundance increases in response to DNA replication stress |
| YER152C |
YER152C |
Uncharacterized protein YER152C; Protein with 2-aminoadipate transaminase activity; shares amino acid similarity with the aminotransferases Aro8p and Aro9p; YER152C is not an essential gene |
| PET122 |
YER153C |
Protein PET122, mitochondrial; Mitochondrial translational activator specific for the COX3 mRNA; acts together with Pet54p and Pet494p; located in the mitochondrial inner membrane |
| OXA1 |
YER154W |
Mitochondrial inner membrane insertase; mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane; also has a role in insertion of carrier proteins into the inner membrane; acts as a voltage-gated ion channel, activated by substrate peptides; interacts with mitochondrial ribosomes; conserved from bacteria to animals |
| BEM2 |
YER155C |
GTPase-activating protein BEM2/IPL2; Rho GTPase activating protein (RhoGAP); involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence; potential GAP for Rho4p |
| MYG1 |
YER156C |
UPF0160 protein YER156C; Putative protein of unknown function; interacts with Hsp82p and copurifies with Ipl1p; expression is copper responsive and downregulated in strains deleted for MAC1, a copper-responsive transcription factor; similarity to mammalian MYG1 |
| COG3 |
YER157W |
Conserved oligomeric golgi complex subunit 3; Essential component of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments |
| YNCE0027C |
YNCE0027C |
Unknown |
| YER158C |
YER158C |
Uncharacterized protein YER158C; Protein of unknown function; potentiy phosphorylated by Cdc28p; YER158C has a paralog, AFR1, that arose from the whole genome duplication |
| BUR6 |
YER159C |
Subunit of a heterodimeric NC2 transcription regulator complex; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha; complex also includes Ncb2p; bur6 ncb2 double mutation is functiony complemented by coexpression of human DRAP1 and DR1, although the single bur6 mutation is not complemented by its ortholog DRAP1 |
| YNCE0028C |
YNCE0028C |
Unknown |
| SPT2 |
YER161C |
Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components; relocalizes to the cytosol in response to hypoxia; similar to mammalian HMG1 proteins |
| RAD4 |
YER162C |
Xeroderma pigmentosum group c-complementing protein; Protein that recognizes and binds damaged DNA (with Rad23p) during NER; subunit of Nuclear Excision Repair Factor 2 (NEF2); also involved, with Rad23p, in turnover of ubiquitylated proteins; Rad4p-Rad23p heterodimer binds to promoters of DNA damage response genes to repress their transcription in the absence of DNA damage; NER stands for nucleotide excision repair; Belongs to the XPC family |
| GCG1 |
YER163C |
Glutathione-specific gamma-glutamylcyclotransferase; Gamma-glutamyl cyclotransferase; cleaves the gamma-glutamyl bond of glutathione to yield 5-oxoproline and a Cys-Gly dipeptide; similar to mammalian pro-apoptotic protein ChaC1; expression of mouse ChaC1 in yeast increases apoptosis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; periodicy expressed during the metabolic cycle |
| CHD1 |
YER164W |
Chromo domain-containing protein 1; Chromatin remodeler that regulates various aspects of transcription; acts in in conjunction with Isw1b to regulate chromatin structure and maintain chromatin integrity during transcription elongation by RNAP II by preventing trans-histone exchange over coding regions; contains a chromo domain, a helicase domain and a DNA-binding domain; component of both the SAGA and SLIK complexes |
| PAB1 |
YER165W |
Polyadenylate-binding protein, cytoplasmic and nuclear; Poly(A) binding protein; part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G; stimulates, but is not required for the deadenylation activity of the Pan2p-Pan3p poly(A)-ribonuclease complex; Belongs to the polyadenylate-binding protein type-1 family |
| DNF1 |
YER166W |
Phospholipid-transporting ATPase DNF1; Aminophospholipid translocase (flippase); type 4 P-type ATPase; involved in phospholipid translocation, contributing to endocytosis, protein transport, and cellular polarization; localizes primarily to the plasma membrane; localizes to the shmoo tip where it has a redundant role in the cellular response to mating pheromone; DNF1 has a paralog, DNF2, that arose from the whole genome duplication |
| BCK2 |
YER167W |
Protein BCK2; Serine/threonine-rich protein involved in PKC1 signaling pathway; protein kinase C (PKC1) signaling pathway controls cell integrity; overproduction suppresses pkc1 mutations |
| CCA1 |
YER168C |
CCA tRNA nucleotidyltransferase, mitochondrial; ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites; human homolog TRNT1 complements yeast null mutant |
| RPH1 |
YER169W |
DNA damage-responsive transcriptional repressor RPH1; JmjC domain-containing histone demethylase; targets tri- and dimethylated H3K36; associates with actively transcribed regions and promotes elongation; repressor of autophagy-related genes in nutrient-replete conditions; damage-responsive repressor of PHR1; phosphorylated by the Rad53p-dependent DNA damage checkpoint pathway and by a Rim1p-mediated event during starvation; target of stress-induced hormesis; RPH1 has a paralog, GIS1, that arose from the whole genome duplication |
| ADK2 |
YER170W |
GTP:AMP phosphotransferase, mitochondrial; Mitochondrial adenylate kinase; catalyzes the reversible synthesis of GTP and AMP from GDP and ADP; may serve as a back-up for synthesizing GTP or ADP depending on metabolic conditions; 3' sequence of ADK2 varies with strain background |
| RAD3 |
YER171W |
5' to 3' DNA helicase; involved in nucleotide excision repair and transcription; subunit of RNA polII initiation factor TFIIH and of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPD protein; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress; Belongs to the helicase family. RAD3/XPD subfamily |
| BRR2 |
YER172C |
Pre-mRNA-splicing helicase BRR2; RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis; homologous to human U5-200kD |
| RAD24 |
YER173W |
Checkpoint protein; involved in the activation of the DNA damage and meiotic pachytene checkpoints; subunit of a clamp loader that loads Rad17p-Mec3p-Ddc1p onto DNA; homolog of human and S. pombe Rad17 protein |
| GRX4 |
YER174C |
Monothiol glutaredoxin-4; Glutathione-dependent oxidoreductase; hydroperoxide and superoxide-radical responsive; monothiol glutaredoxin subfamily member along with Grx3p and Grx5p; protects cells from oxidative damage; with Grx3p, binds to Aft1p in iron-replete conditions, promoting its dissociation from promoters; mutant has increased aneuploidy tolerance; transcription regulated by Yap5p; GRX4 has a paralog, GRX3, that arose from the whole genome duplication |
| TMT1 |
YER175C |
Trans-aconitate methyltransferase; cytosolic enzyme that catalyzes the methyl esterification of 3-isopropylmalate, an intermediate of the leucine biosynthetic pathway, and trans-aconitate, which inhibits the citric acid cycle; Belongs to the methyltransferase superfamily. Tam family |
| YER175W-A |
YER175W-A |
Uncharacterized protein YER175W-A; Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching |
| ECM32 |
YER176W |
Putative ATP-dependent RNA helicase ECM32; DNA dependent ATPase/DNA helicase; helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes |
| BMH1 |
YER177W |
14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of exocytosis, vesicle transport, Ras/MAPK and rapamycin-sensitive signaling, aggresome formation, spindle position checkpoint; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress; antiapoptotic gene similar to human 14-3-3; BMH1 has a paralog, BMH2, that arose from whole genome duplication |
| PDA1 |
YER178W |
E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex; catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA; phosphorylated; regulated by glucose; PDH complex is concentrated in spots within the mitochondrial matrix, often near the ERMES complex and near peroxisomes |
| DMC1 |
YER179W |
Meiotic recombination protein DMC1; Meiosis-specific recombinase required for double-strand break repair; also required for pairing between homologous chromosomes; required for the normal morphogenesis of synaptonemal complex; homolog of Rad51p and the bacterial RecA protein; binds ssDNA and dsDNA, forms helical filaments; stimulated by Rdh54p; Belongs to the RecA family. DMC1 subfamily |
| ISC10 |
YER180C |
Meiosis-specific protein ISC10; Protein required for sporulation; transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells |
| SLO1 |
YER180C-A |
SCOCO-like protein 1; Protein interacting with Arl3p; Arl3p is a GTPase of the Ras superfamily involved in vesicle-tethering at the Golgi; putative ortholog of human SCOCO |
| YNCE0029C |
YNCE0029C |
Unknown |
| YER181C |
YER181C |
Mitochondrial protein of unknown function; conserved among S. cerevisiae strains; extensively overlaps a Ty1 LTR; YER181C is not an essential gene |
| FMP10 |
YER182W |
Uncharacterized mitochondrial membrane protein FMP10; Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; Belongs to the FMP10 family |
| FAU1 |
YER183C |
5-formyltetrahydrofolate cyclo-ligase; 5,10-methenyltetrahydrofolate synthetase; involved in folic acid biosynthesis |
| TOG1 |
YER184C |
Uncharacterized transcriptional regulatory protein YER184C; Transcriptional activator of oleate genes; regulates genes involved in fatty acid utilization; zinc cluster protein; deletion confers sensitivity to Calcufluor white, and prevents growth on glycerol or lactate as sole carbon source |
| PUG1 |
YER185W |
Protoporphyrin uptake protein 1; Plasma membrane protein involved in protoprophyrin and heme transport; roles in the uptake of protoprophyrin IX and the efflux of heme; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of proteins |
| YER186C |
YER186C |
Uncharacterized protein YER186C; Putative protein of unknown function |
| YER187W |
YER187W |
Uncharacterized protein YER187W; Putative protein of unknown function; induced in respiratory-deficient cells; To yeast killer toxin KHS and to YGL262w |
| YER188W |
YER188W |
Uncharacterized protein YER188W; Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; large-scale analyses show mRNA expression increases under anaerobic conditions and two-hybrid interactions with Sst2p |
| YER188C-A |
YER188C-A |
UPF0320 protein YER188C-A; Putative protein of unknown function; Belongs to the UPF0320 family |
| YLR464W |
YLR464W |
Putative protein of unknown function; intron is predicted but not detected experimenty; YLR464W overlaps the verified gene YRF1-4/YLR466W and two dubious ORFs YLR463C and YLR465C; Belongs to the helicase family. Yeast subtelomeric Y' repeat subfamily |
| AAD15 |
YOL165C |
Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role; AAD15 has a paralog, AAD3, that arose from a segmental duplication; members of the AAD gene family comprise three pairs (AAD3 + AAD15, AAD6/AAD16 + AAD4, AAD10 + AAD14) whose two genes are more related to one another than to other members of the family |
| AGP3 |
YFL055W |
Low-affinity amino acid permease; may act to supply the cell with amino acids as nitrogen source in nitrogen-poor conditions; transcription is induced under conditions of sulfur limitation; plays a role in regulating Ty1 transposition |
| AQY3 |
YFL054C |
Aquaglyceroporin related protein, other eukaryote; Uncharacterized membrane protein YFL054C; Putative channel-like protein; similar to Fps1p; mediates passive diffusion of glycerol in the presence of ethanol; Belongs to the MIP/aquaporin (TC 1.A.8) family |
| DAK2 |
YFL053W |
Triose/dihydroxyacetone kinase / fad-amp lyase (cyclizing); Dihydroxyacetone kinase; required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation; Belongs to the dihydroxyacetone kinase (DAK) family |
| ZNF1 |
YFL052W |
Uncharacterized transcriptional regulatory protein YFL052W; Zinc cluster transcription factor that regulates respiratory growth; binds to promoters of genes involved in respiration, gluconeogenesis, and the glyoxylate shunt; required for normal carbon source utilization and stress response |
| YFL051C |
YFL051C |
Uncharacterized membrane protein YFL051C; Putative protein of unknown function; SWAT-GFP fusion protein localizes to the cell periphery while mCherry fusion protein localizes to both the cell periphery and vacuole; YFL051C is not an essential gene |
| ALR2 |
YFL050C |
Magnesium transporter ALR2; Probable Mg(2+) transporter; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions; plays a role in regulating Ty1 transposition |
| SWP82 |
YFL049W |
SWI/SNF global transcription activator complex subunit SWP82; Member of the SWI/SNF chromatin remodeling complex; has an as yet unidentified role in the complex; has identifiable counterparts in closely related yeast species; abundantly expressed in many growth conditions; paralog of Npl6p; relocates to the cytosol under hypoxic conditions; Belongs to the RSC7/SWP82 family. SWP82 subfamily |
| EMP47 |
YFL048C |
Lectin, mannose-binding 1; Protein EMP47; Integral membrane component of ER-derived COPII-coated vesicles; functionS in ER to Golgi transport; EMP47 has a paralog, EMP46, that arose from the whole genome duplication |
| RGD2 |
YFL047W |
Rho-GTPase-activating protein RGD2; GTPase-activating protein (RhoGAP) for Cdc42p and Rho5p; relocalizes from bud neck to cytoplasm upon DNA replication stress |
| FMP32 |
YFL046W |
Protein FMP32, mitochondrial; Putative assembly factor for cytochrome c oxidase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; has similarity to human MCUR1/CCDC90A |
| SEC53 |
YFL045C |
Phosphomannomutase; involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen; Belongs to the eukaryotic PMM family |
| OTU1 |
YFL044C |
Ubiquitin thioesterase OTU1; Deubiquitylation enzyme that binds to the chaperone-ATPase Cdc48p; may contribute to regulation of protein degradation by deubiquitylating substrates that have been ubiquitylated by Ufd2p; member of the Ovarian Tumor (OTU) family; protein abundance increases in response to DNA replication stress |
| LAM5 |
YFL042C |
Membrane-anchored lipid-binding protein LAM5; Putative sterol transfer protein; one of six StART-like domain-containing proteins in yeast that may be involved in sterol transfer between intracellular membranes; conserved across eukaryotes; has both GRAM and StART-like (VASt) domains; localizes to membrane contact sites throughout the cell, including nucleus-vacuole junctions and ER-mitochondrial contact sites |
| YFL041W-A |
YFL041W-A |
Uncharacterized protein YFL041W-A; Putative protein of unknown function; identified by fungal homology and RT-PCR |
| FET5 |
YFL041W |
Iron transport multicopper oxidase FET5; Multicopper oxidase; integral membrane protein with similarity to Fet3p; may have a role in iron transport |
| YFL040W |
YFL040W |
Probable metabolite transport protein YFL040W; Putative transporter; member of the sugar porter family; YFL040W is not an essential gene; may have a role in intracellular sterol transport |
| ACT1 |
YFL039C |
Actin, other eukaryote; Actin; structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions |
| YPT1 |
YFL038C |
GTP-binding protein YPT1; Rab family GTPase; involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by type II protein geranylgeranyltransferase (Bet2p-Bet4p); binds to unspliced HAC1 mRNA; regulates the unfolded protein response (UPR) by promoting the decay of HAC1 RNA; localizes to the early Golgi, the transitional Golgi and ER membranes, pre-autophagosomal structures, and cytoplasmic vesicles |
| TUB2 |
YFL037W |
Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria |
| RUF21 |
YNCF0001C |
Unknown |
| RPO41 |
YFL036W |
Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation; Rpo41p also synthesizes RNA primers for mitochondrial DNA replication |
| MOB2 |
YFL034C-B |
Activator of Cbk1p kinase; component of the RAM signaling network that regulates cellular polarity and morphogenesis; activation of Cbk1p facilitates the Ace2p-dependent daughter cell-specific transcription of genes involved in cell separation; similar to Mob1p |
| RPL22B |
YFL034C-A |
Ribosomal 60S subunit protein L22A; required for translation of long 5' UTR of IME1 mRNA and meiotic entry; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22B has a paralog, RPL22A, that arose from the whole genome duplication |
| MIL1 |
YFL034W |
Uncharacterized membrane protein YFL034W; Predicted lipase; binds variant medium clathrin chain Apm2p and contributes to its membrane recruitment; putative integral membrane protein that interacts with Rpp0p component of ribosomal stalk |
| RIM15 |
YFL033C |
Serine/threonine-protein kinase RIM15; Protein kinase involved in cell proliferation in response to nutrients; glucose-repressible; involved in signal transduction during cell proliferation in response to nutrients, specificy the establishment of stationary phase; identified as a regulator of IME2; phosphorylates Igo1p and Igo2p; substrate of Pho80p-Pho85p kinase |
| HAC1 |
YFL031W |
Transcriptional activator HAC1; Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; two functional forms of Hac1p are produced; translation initiation is repressed under non-stress conditions; protein abundance increases in response to DNA replication stress |
| AGX1 |
YFL030W |
Alanine-glyoxylate transaminase / serine-glyoxylate transaminase / serine-pyruvate transaminase; Alanine--glyoxylate aminotransferase 1; Alanine:glyoxylate aminotransferase (AGT); catalyzes the synthesis of glycine from glyoxylate, which is one of three pathways for glycine biosynthesis in yeast; similar to mammalian and plant alanine:glyoxylate aminotransferases; human homolog AGXT complements yeast null mutant; Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family |
| CAK1 |
YFL029C |
Serine/threonine-protein kinase CAK1; Cyclin-dependent kinase-activating kinase; required for passage through the cell cycle; phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases; Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily |
| CAF16 |
YFL028C |
CCR4-associated factor 16; Part of evolutionarily-conserved CCR4-NOT regulatory complex; contains single ABC-type ATPase domain but no transmembrane domain; interacts with several subunits of Mediator; Belongs to the ABC transporter superfamily |
| GYP8 |
YFL027C |
GTPase-activating protein for yeast Rab family members; Ypt1p is the preferred in vitro substrate but also acts on Sec4p, Ypt31p and Ypt32p; involved in the regulation of ER to Golgi vesicle transport |
| STE2 |
YFL026W |
Receptor for alpha-factor pheromone; seven transmembrane-domain GPCR that interacts with both pheromone and a heterotrimeric G protein to initiate the signaling response that leads to mating between haploid a and alpha cells; Belongs to the G-protein coupled receptor 4 family |
| BST1 |
YFL025C |
GPI inositol deacylase of the endoplasmic reticulum (ER); negatively regulates COPII vesicle formation; prevents production of vesicles with defective subunits; required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules; functional ortholog of human PGAP1, mutation of which is associated with intellectual disability and encephalopathy |
| EPL1 |
YFL024C |
Enhancer of polycomb-like protein 1; Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb; coding sequence contains length polymorphisms in different strains |
| BUD27 |
YFL023W |
Bud site selection protein 27; Unconventional prefoldin protein involved in translation initiation; required for correct assembly of RNAP I, II, and III in an Rpb5p-dependent manner; shuttles between nucleus and cytoplasm; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP |
| FRS2 |
YFL022C |
Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase; forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar; Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 2 subfamily |
| GAT1 |
YFL021W |
Transcriptional regulatory protein GAT1; Transcriptional activator of nitrogen catabolite repression genes; contains a GATA-1-type zinc finger DNA-binding motif; activity and localization regulated by nitrogen limitation and Ure2p; different translational starts produce two major and two minor isoforms that are differentiy regulated and localized |
| PAU5 |
YFL020C |
Seripauperin-5; Member of the seripauperin multigene family; encoded mainly in subtelomeric regions; induced during alcoholic fermentation; induced by low temperature and also by anaerobic conditions; negatively regulated by oxygen and repressed by heme |
| YFL019C |
YFL019C |
Uncharacterized protein YFL019C; Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YFL019C is not an essential gene |
| SUF9 |
YNCF0002W |
Unknown |
| LPD1 |
YFL018C |
Dihydrolipoyl dehydrogenase, mitochondrial; Dihydrolipoamide dehydrogenase; the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multi-enzyme complexes; PDH complex is concentrated in spots within the mitochondrial matrix, often near the ERMES complex and near peroxisomes; LPD1 has a paralog, IRC15, that arose from the whole genome duplication |
| SMX2 |
YFL017W-A |
Sm nuclear ribonucleoprotein G; Core Sm protein Sm G; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx3p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm G |
| GNA1 |
YFL017C |
Glucosamine-6-phosphate acetyltransferase; evolutionarily conserved; required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA; Belongs to the acetyltransferase family. GNA1 subfamily |
| MDJ1 |
YFL016C |
DnaJ homolog 1, mitochondrial; Co-chaperone that stimulates HSP70 protein Ssc1p ATPase activity; involved in protein folding/refolding in the mitochodrial matrix; required for proteolysis of misfolded proteins; member of the HSP40 (DnaJ) family of chaperones |
| YFL015C |
YFL015C |
Uncharacterized protein YFL015C; Putative protein of unknown function; conserved across S. cerevisiae strains; partiy overlaps dubious ORF YFL015W-A; YFL015C is not an essential gene |
| HSP12 |
YFL014W |
12 kDa heat shock protein; Plasma membrane protein involved in maintaining membrane organization; involved in maintaining organization during stress conditions; induced by heat shock, oxidative stress, osmostress, stationary phase, glucose depletion, oleate and alcohol; protein abundance increased in response to DNA replication stress and dietary restriction; regulated by the HOG and Ras-Pka pathways; required for dietary restriction-induced lifespan extension |
| IES1 |
YFL013C |
Ino eighty subunit 1; Subunit of the INO80 chromatin remodeling complex; relocalizes to the cytosol in response to hypoxia |
| YFL012W |
YFL012W |
Uncharacterized protein YFL012W; Putative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin |
| HXT10 |
YFL011W |
Mfs transporter, sp family, sugar:h+ symporter; Putative hexose transporter; expressed at low levels and expression is repressed by glucose |
| AUA1 |
YFL010W-A |
Protein required for the negative regulation by ammonia of Gap1p; Gap1p is a general amino acid permease |
| WWM1 |
YFL010C |
WW domain containing protein of unknown function; binds to Mca1p, a caspase-related protease that regulates H2O2-induced apoptosis; overexpression causes G1 phase growth arrest and clonal death that is suppressed by overexpression of MCA1 |
| CDC4 |
YFL009W |
Cell division control protein 4; F-box protein required for both the G1/S and G2/M phase transitions; modular substrate specificity factor which associates with core SCF (Cdc53p, Skp1p and Hrt1p/Rbx1p) to form the SCFCdc4 complex; SCFCdc4 acts as a ubiquitin-protein ligase directing ubiquitination of cyclin-dependent kinase (CDK) phosphorylated substrates, such as: Sic1p, Far1p, Cdc6p, Clb6p, and Cln3p |
| SMC1 |
YFL008W |
Structural maintenance of chromosomes protein 1; Subunit of the multiprotein cohesin complex; essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure; Belongs to the SMC family. SMC1 subfamily |
| BLM10 |
YFL007W |
Proteasome activator; binds the core proteasome (CP) and stimulates proteasome-mediated protein degradation by inducing gate opening; required for sequestering CP into proteasome storage granule (PSG) during quiescent phase and for nuclear import of CP in proliferating cells; required for resistance to bleomycin, may be involved in protecting against oxidative damage; similar to mammalian PA200 |
| SEC4 |
YFL005W |
Ras-related protein SEC4; Rab family GTPase; essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane |
| RUF20 |
YNCF0003C |
Unknown |
| VTC2 |
YFL004W |
Regulatory subunit of the vacuolar transporter chaperone (VTC) complex; involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion; VTC2 has a paralog, VTC3, that arose from the whole genome duplication; Belongs to the VTC2/3 family |
| MSH4 |
YFL003C |
MutS protein homolog 4; Protein involved in meiotic recombination; required for normal levels of crossing over, colocalizes with Zip2p to discrete foci on meiotic chromosomes, has homology to bacterial MutS protein; Belongs to the DNA mismatch repair MutS family |
| YNCF0004C |
YNCF0004C |
Unknown |
| SPB4 |
YFL002C |
Putative ATP-dependent RNA helicase; nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients; Belongs to the DEAD box helicase family. DDX55/SPB4 subfamily |
| DEG1 |
YFL001W |
tRNA:pseudouridine synthase; introduces pseudouridines at position 38 or 39 in tRNA; also responsible for pseudouracil modification of some mRNAs; important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability |
| LOC1 |
YFR001W |
60S ribosomal subunit assembly/export protein LOC1; Nuclear protein involved in asymmetric localization of ASH1 mRNA; binds double-stranded RNA in vitro; constituent of 66S pre-ribosomal particles; required at post-transcriptional step for efficient retrotransposition; absence results in decreased Ty1 Gag:GFP protein levels; relocalizes from nucleus to cytoplasm upon DNA replication stress |
| NIC96 |
YFR002W |
Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) |
| YPI1 |
YFR003C |
Regulatory subunit of the type I protein phosphatase (PP1) Glc7p; Glc7p participates in the regulation of a variety of metabolic processes including mitosis and glycogen metabolism; in vitro evidence suggests Ypi1p is an inhibitor of Glc7p while in vivo evidence suggests it is an activator; overproduction causes decreased cellular content of glycogen; partial depletion causes lithium sensitivity, while overproduction confers lithium-tolerance |
| RPN11 |
YFR004W |
Ubiquitin carboxyl-terminal hydrolase RPN11; Metoprotease subunit of 19S regulatory particle; part of 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress |
| SAD1 |
YFR005C |
U4/U6.U5 tri-snRNP-associated protein 2; Pre-mRNA-splicing factor SAD1; Conserved zinc-finger domain protein involved in pre-mRNA splicing; critical for splicing of nearly intron-containing genes; required for assembly of U4 snRNA into the U4/U6 particle |
| YFR006W |
YFR006W |
Uncharacterized peptidase YFR006W; Putative X-Pro aminopeptidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YFR006W is not an essential gene |
| YNCF0005C |
YNCF0005C |
Unknown |
| YFH7 |
YFR007W |
ATP-dependent kinase YFH7; Putative kinase with similarity to the PRK/URK/PANK kinase subfamily; the PRK/URK/PANK subfamily of P-loop kinases is also known as phosphoribulokinase/uridine kinase/bacterial pantothenate kinase; Belongs to the YFH7 family |
| FAR7 |
YFR008W |
Factor arrest protein 7; Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress |
| GCN20 |
YFR009W |
Protein GCN20; Positive regulator of the Gcn2p kinase activity; forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA; Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily |
| UBP6 |
YFR010W |
Ubiquitin-specific protease; situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains en bloc, rather than from the distal tip of the chain; negatively regulates degradation of ubiquitinated proteins by the proteasome; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance; human homolog UBP14 complements yeast null mutant |
| MIC19 |
YFR011C |
Component of the MICOS complex; MICOS (formerly MINOS or MitOS) is a mitochondrial inner membrane complex that extends into the intermembrane space and has a role in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane; Mic19p is peripheral to the inner membrane and may connect Mic60p with the Mic10p-Mic12p-Mic27p subcomplex; both yeast and human Mic19p become oxidized, and oxidation may regulate MICOS |
| SUP11 |
YNCF0007W |
Unknown |
| DCV1 |
YFR012W |
Protein of unknown function; deletion mutant shows strong genetic interaction with cdc28-as1 mutant in the presence of 1-NM-PP1; DCV1 has a paralog, YOL019W, that arose from the whole genome duplication |
| YFR012W-A |
YFR012W-A |
Uncharacterized protein YFR012W-A; Putative protein of unknown function; identified by homology |
| IOC3 |
YFR013W |
ISWI one complex protein 3; Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; promotes nucleosome shifts in the 5 prime direction; IOC3 has a paralog, ESC8, that arose from the whole genome duplication |
| CMK1 |
YFR014C |
Calmodulin-dependent protein kinase; may play a role in stress response, many Ca++/calmodulin dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK1 has a paralog, CMK2, that arose from the whole genome duplication; Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily |
| GSY1 |
YFR015C |
Glycogen [starch] synthase isoform 1; Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, environmental stress, and entry into stationary phase; GSY1 has a paralog, GSY2, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress |
| AIP5 |
YFR016C |
Uncharacterized protein YFR016C; Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and bud; interacts with Spa2p; YFR016C is not an essential gene |
| IGD1 |
YFR017C |
Cytoplasmic protein that inhibits Gdb1p glycogen debranching activity; required for normal intracellular accumulation of glycogen; phosphorylated in vivo; expression increases during wine fermentation; protein abundance increases in response to DNA replication stress; IGD1 has a paralog, YOL024W, that arose from the whole genome duplication |
| YFR018C |
YFR018C |
Glutaminyl-peptide cyclotransferase; Uncharacterized protein YFR018C; Putative protein of unknown function; SWAT-GFP and seamless GFP fusion proteins localize to the endoplasmic reticulum and mCherry fusion protein localizes to the vacuole |
| FAB1 |
YFR019W |
1-phosphatidylinositol 3-phosphate 5-kinase FAB1; 1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis |
| YNCF0009C |
YNCF0009C |
Unknown |
| CSS2 |
YFR020W |
Uncharacterized protein YFR020W; Protein of unknown function, secreted when constitutively expressed; SWAT-GFP fusion protein localizes to the endoplasmic reticulum (ER) and extracellular region, while mCherry fusion protein localizes to the ER and vacuole; mRNA identified as translated by ribosome profiling data; CSS2 is a non-essential gene |
| ATG18 |
YFR021W |
Autophagy-related protein 18; Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein; relocalizes from vacuole to cytoplasm upon DNA replication stress; has 4 mammalian homologs WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood |
| ROG3 |
YFR022W |
Arrestin-related trafficking adapter 4/5/7; Protein ROG3; Alpha-arrestin involved in ubiquitin-dependent endocytosis; contributes to desensitization of agonist-occupied alpha-factor receptor Ste2p by Rsp5p-independent internalization; PPXY motif-mediated binding of the ubiquitin ligase Rsp5p is not required for adaptation; mutation suppresses the temperature sensitivity of an mck1 rim11 double mutant; SWAT-GFP and mCherry fusion proteins localize to the cytosol; ROG3 has a paralog, ROD1, that arose from the whole genome duplication |
| RUF22 |
YNCF0010C |
Unknown |
| PES4 |
YFR023W |
Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication |
| LSB3 |
YFR024C-A |
Protein containing a C-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein abundance increases in response to DNA replication stress; LSB3 has a paralog, YSC84, that arose from the whole genome duplication |
| HIS2 |
YFR025C |
Histidinol-phosphatase; Histidinolphosphatase; catalyzes the eighth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control |
| YNCF0011C |
YNCF0011C |
Unknown |
| ULI1 |
YFR026C |
Protein of unknown function; involved in and induced by the endoplasmic reticulum unfolded protein response (UPR); SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum |
| ECO1 |
YFR027W |
N-acetyltransferase ECO1; Acetyltransferase; required for establishment of sister chromatid cohesion; acetylates Mps3p to regulate nuclear organization; modifies Smc3p at replication forks and Mcd1p in response to dsDNA breaks; phosphorylated by three kinases (Cdc28p, Cdc7p, Mck1p) to generate pair of phosphates spaced precisely for recognition by ubiquitin ligase SCF-Cdc4; mutations in human homolog ESCO2 cause Roberts syndrome; relative distribution to nucleus increases upon DNA replication stress |
| CDC14 |
YFR028C |
Tyrosine-protein phosphatase CDC14; Protein phosphatase required for mitotic exit; required for rDNA segregation, cytokinesis, meiosis I spindle disassembly, environmental stress response; held in nucleolus by Cdc55p in early meiosis, liberated by FEAR and Mitotic Exit Network in anaphase, enabling it to effect a decrease in CDK/B-cyclin activity and mitotic exit; sequestered in metaphase II, released upon entry into anaphase II; human homolog CDC14A can complement thermosensitivity of yeast cdc14-1 mutant |
| SUP6 |
YNCF0012C |
Unknown |
| PTR3 |
YFR029W |
SPS-sensor component PTR3; Component of the SPS plasma membrane amino acid sensor system; senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes; other members are Ssy1p, Ptr3p, and Ssy5p |
| MET10 |
YFR030W |
Sulfite reductase [NADPH] flavoprotein component; Subunit alpha of assimilatory sulfite reductase; complex converts sulfite into sulfide |
| SMC2 |
YFR031C |
Structural maintenance of chromosomes protein 2; Subunit of the condensin complex; condensin reorganizes chromosomes during both mitosis and meiosis; essential SMC chromosomal ATPase family member that forms a subcomplex with Smc2p that has ATP-hydrolyzing and DNA-binding activity, but other condensin subunits are required for chromatin binding; required for clustering of tRNA genes at the nucleolus |
| RPL2A |
YFR031C-A |
Ribosomal 60S subunit protein L2A; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2A has a paralog, RPL2B, that arose from the whole genome duplication |
| RUF23 |
YNCF0013W |
Unknown |
| RRT5 |
YFR032C |
Regulator of rDNA transcription protein 5; Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; expressed at high levels during sporulation |
| RPL29 |
YFR032C-A |
Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and sm ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog; Belongs to the eukaryotic ribosomal protein eL29 family |
| MIN10 |
YFR032C-B |
Uncharacterized protein YFR032C-B; Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching |
| QCR6 |
YFR033C |
Subunit 6 of the ubiquinol cytochrome-c reductase complex; the complex, also known as the cytochrome bc(1) complex or Complex III, is a component of the mitochondrial inner membrane electron transport chain; highly acidic protein; required for maturation of cytochrome c1; may be loosely associated with the complex since it is easily released into the intermembrane space; Belongs to the UQCRH/QCR6 family |
| PHO4 |
YFR034C |
Phosphate system positive regulatory protein PHO4; Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p; function is regulated by phosphorylation at multiple sites and by phosphate availability |
| YFR035C |
YFR035C |
Uncharacterized protein YFR035C; Putative protein of unknown function; deletion mutant exhibits synthetic phenotype with alpha-synuclein |
| YNCF0014C |
YNCF0014C |
Unknown |
| CDC26 |
YFR036W |
Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; relocalizes to the cytosol in response to hypoxia; Belongs to the CDC26 family |
| RSC8 |
YFR037C |
Chromatin structure-remodeling complex protein RSC8; Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters |
| IRC5 |
YFR038W |
Uncharacterized ATP-dependent helicase IRC5; Putative ATPase containing the DEAD/H helicase-related sequence motif; null mutant displays increased levels of spontaneous Rad52p foci; SWAT-GFP and mCherry fusion proteins localize to the nucleus |
| OSW7 |
YFR039C |
Protein involved in outer spore w assembly; likely involved directly in dityrosine layer assembly; may be involved in response to high salt and changes in carbon source; SWAT-GFP, seamless-GFP and mCherry fusion proteins localize to the endoplasmic reticulum; deletion mutant has decreased spore survival in Drosophila feces; OSW7 has a paralog, SHE10, that arose from the whole genome duplication; paralogs are redundant for spore w dityrosine assembly |
| SAP155 |
YFR040W |
SIT4-associating protein SAP155; Protein required for function of the Sit4p protein phosphatase; forms a complex with Sit4p; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p; protein abundance increases in response to DNA replication stress; SAP155 has a paralog, SAP4, that arose from the whole genome duplication; Belongs to the SAPS family |
| ERJ5 |
YFR041C |
ER-localized J domain-containing protein 5; Type I membrane protein with a J domain; required to preserve the folding capacity of the endoplasmic reticulum; loss of the non-essential ERJ5 gene leads to a constitutively induced unfolded protein response |
| KEG1 |
YFR042W |
Beta-1,6-glucan synthesis-associated protein KEG1; Integral membrane protein of the ER; physicy interacts with Kre6p; has a role in the synthesis of beta-1,6-glucan in the cell w; required for cell viability |
| IRC6 |
YFR043C |
Increased recombination centers protein 6; Clathrin coat accessory factor; involved in clathrin-mediated vesicle trafficking; may function to link the AP-1 clathrin adaptor complex with the Rab GTPase Ypt31p; has structural similarity to G-proteins; mouse homolog Aagab (p34) functiony complements irc6 null mutation; null mutant displays increased levels of spontaneous Rad52p foci |
| DUG1 |
YFR044C |
Cys-Gly metodipeptidase DUG1; Cys-Gly meto-di-peptidase; forms a complex with Dug2p and Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p); human homolog CNDP2 can complement yeast dug1 mutant |
| MRX20 |
YFR045W |
Solute carrier family 25 (mitochondrial citrate transporter), member 1; Uncharacterized mitochondrial carrier YFR045W; Putative mitochondrial transport protein; null mutant is viable, exhibits decreased levels of chitin and normal resistance to calcofluor white |
| CNN1 |
YFR046C |
Kinetochore-associated protein CNN1; Kinetochore protein; associated with the essential kinetochore proteins Nnf1p and Spc24p; phosphorylated by Clb5-Cdk1, Mps1p, Ipl1p and to a lesser extent by Clb2-Cdk1; localizes to the lower region of the Ndc80 complex during anaphase and regulates KMN activity by inhibiting the Mtw1 and Spc105 complexes from binding to the Ndc80 complex; similar to metazoan CENP-T |
| BNA6 |
YFR047C |
Nicotinate-nucleotide pyrophosphorylase [carboxylating]; Quinolinate phosphoribosyl transferase; required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p; Belongs to the NadC/ModD family |
| RMD8 |
YFR048W |
Sporulation protein rmd8; Cytosolic protein required for sporulation |
| YMR31 |
YFR049W |
37S ribosomal protein YMR-31, mitochondrial; Subunit of the mitochondrial alpha-ketoglutarate dehydrogenase; recruits E3 subunit (Lpd1p) to the E1-E2 (Kgd1p, Kgd2p) core; has similarity to human mitochondrial ribosomal protein MRP-S36 |
| PRE4 |
YFR050C |
Proteasome core particle subunit beta 7; Beta 7 subunit of the 20S proteasome; Belongs to the peptidase T1B family |
| RET2 |
YFR051C |
Delta subunit of the coatomer complex (COPI); COPI coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER; Belongs to the adaptor complexes medium subunit family. Delta-COP subfamily |
| RPN12 |
YFR052W |
Subunit of the 19S regulatory particle of the 26S proteasome lid; syntheticy lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physicy interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress |
| HXK1 |
YFR053C |
Hexokinase-1; Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication |
| YFR054C |
YFR054C |
Uncharacterized protein YFR054C; Putative protein of unknown function; conserved among S. cerevisiae strains |
| IRC7 |
YFR055W |
Putative cystathionine beta-lyase; Beta-lyase involved in the production of thiols; null mutant displays increased levels of spontaneous Rad52p foci; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner and by copper levels in a Mac1-dependent manner; Belongs to the trans-sulfuration enzymes family |
| PAU1 |
YJL223C |
Seripauperin-14; Member of the seripauperin multigene family; encoded mainly in subtelomeric regions; SWAT-GFP and mCherry fusion proteins localize to the vacuole; active during alcoholic fermentation; regulated by anaerobiosis, negatively regulated by oxygen; repressed by heme; identical to Pau14p; Belongs to the SRP1/TIP1 family. Seripauperin subfamily |
| COS12 |
YGL263W |
Endosomal protein involved in turnover of plasma membrane proteins; member of the DUP380 subfamily of conserved, often subtelomeric COS genes; required for the multivesicular vesicle body sorting pathway that internalizes plasma membrane proteins for degradation; Cos proteins provide ubiquitin in trans for nonubiquitinated cargo proteins |
| YGL262W |
YGL262W |
Putative protein of unknown function; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein; YGL262W is not an essential gene; To yeast YER187w |
| PAU14 |
YIL176C |
Protein of unknown function; member of the seripauperin multigene family encoded mainly in subtelomeric regions; identical to Pau1p; Belongs to the SRP1/TIP1 family. Seripauperin subfamily |
| PAU9 |
YBL108C-A |
Protein of unknown function; member of the seripauperin multigene family encoded mainly in subtelomeric regions; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum and vacuole respectively |
| YGL258W-A |
YGL258W-A |
Uncharacterized protein YGL258W-A; Putative protein of unknown function |
| VEL1 |
YGL258W |
Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has a paralog, YOR387C, that arose from a single-locus duplication |
| MNT2 |
YGL257C |
Alpha-1,3-mannosyltransferase MNT2; Mannosyltransferase; involved in adding the 4th and 5th mannose residues of O-linked glycans; Belongs to the MNN1/MNT family |
| ADH4 |
YGL256W |
Alcohol dehydrogenase isoenzyme type IV; dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency |
| ZRT1 |
YGL255W |
Solute carrier family 39 (zinc transporter), member 1/2/3; Zinc-regulated transporter 1; High-affinity zinc transporter of the plasma membrane; responsible for the majority of zinc uptake; transcription is induced under low-zinc conditions by the Zap1p transcription factor; Belongs to the ZIP transporter (TC 2.A.5) family |
| FZF1 |
YGL254W |
Zinc finger protein FZF1; Transcription factor involved in sulfite metabolism; sole identified regulatory target is SSU1; overexpression suppresses sulfite-sensitivity of many unrelated mutants due to hyperactivation of SSU1, contains five zinc fingers; protein abundance increases in response to DNA replication stress |
| HXK2 |
YGL253W |
Hexokinase-2; Hexokinase isoenzyme 2; phosphorylates glucose in cytosol; predominant hexokinase during growth on glucose; represses expression of HXK1, GLK1, induces expression of its own gene; antiapoptotic; phosphorylation/dephosphorylation at Ser14 by kinase Snf1p, phosphatase Glc7p-Reg1p regulates nucleocytoplasmic shuttling of Hxk2p; functions downstream of Sit4p in control of cell cycle, mitochondrial function, oxidative stress resistance, chronological lifespan; has paralog HXK1 |
| RTG2 |
YGL252C |
Retrograde regulation protein 2; Sensor of mitochondrial dysfunction; regulates the subcellular location of Rtg1p and Rtg3p, transcriptional activators of the retrograde (RTG) and TOR pathways; Rtg2p is inhibited by the phosphorylated form of Mks1p |
| HFM1 |
YGL251C |
Meiosis specific DNA helicase; involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity |
| RMR1 |
YGL250W |
Protein required for meiotic recombination and gene conversion; null mutant displays reduced PIS1 expression and growth defects on non-fermentable carbon sources and minimal media; GFP-fusion protein localizes to both cytoplasm and nucleus; Belongs to the RMR1 family |
| ZIP2 |
YGL249W |
Meiosis-specific protein; involved in normal synaptonemal complex formation and pairing between homologous chromosomes during meiosis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress; Belongs to the ZIP2 family |
| RME3 |
YNCG0001C |
Unknown |
| PDE1 |
YGL248W |
3',5'-cyclic-nucleotide phosphodiesterase 1; Low-affinity cyclic AMP phosphodiesterase; controls glucose and intracellular acidification-induced cAMP signaling, target of the cAMP-protein kinase A (PKA) pathway; glucose induces transcription and inhibits translation |
| BRR6 |
YGL247W |
Nucleus export protein BRR6; Essential nuclear envelope integral membrane protein; interacts and functions with Apq12p and Brl1p in lipid homeostasis; mutants are defective in nuclear pore complex biogenesis, nuclear envelope morphology, mRNA nuclear export, and are sensitive to sterol biosynthesis inhibitors and membrane fluidizing agents; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism; homologous to Brl1p; Belongs to the BRL1/BRR6 family |
| RAI1 |
YGL246C |
Nuclear protein with decapping endonuclease activity; targets mRNAs with unmethylated 7-methylguanosine cap structures and 5'-triphosphates; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; relocalizes to the cytosol in response to hypoxia; homologous to human DOM3Z; Belongs to the DXO/Dom3Z family |
| GUS1 |
YGL245W |
Glutamate--tRNA ligase, cytoplasmic; Glutamyl-tRNA synthetase (GluRS); forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; complex formation increases the catalytic efficiency of both tRNA synthetases and ensures their correct localization to the cytoplasm; protein abundance increases in response to DNA replication stress; Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 2 subfamily |
| RTF1 |
YGL244W |
RNA polymerase-associated protein RTF1; Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay |
| TAD1 |
YGL243W |
tRNA-specific adenosine deaminase; deaminates adenosine-37 to inosine in tRNA-Ala; Belongs to the ADAT1 family |
| YGL242C |
YGL242C |
Ankyrin repeat-containing protein YGL242C; Protein of unknown function; N-terminy propionylated in vivo; deletion mutant is viable |
| KAP114 |
YGL241W |
Importin subunit beta-5; Karyopherin, responsible for nuclear import of specific proteins; cargoes include Spt15p, Sua7p, histones H2A and H2B, and Nap1p; amino terminus shows similarity to those of other importins, particularly Cse1p; localization is primarily nuclear; function is regulated by sumoylation; protein abundance increases in response to DNA replication stress |
| DOC1 |
YGL240W |
Anaphase-promoting complex subunit DOC1; Processivity factor; required for the ubiquitination activity of the anaphase promoting complex (APC), mediates the activity of the APC by contributing to substrate recognition; involved in cyclin proteolysis; contains a conserved DOC1 homology domain; Belongs to the APC10 family |
| CSE1 |
YGL238W |
Importin alpha re-exporter; Nuclear envelope protein that acts as a recycling factor; mediates the nuclear export of Srp1p (importin alpha) back to the cytoplasm after its import substrates have been released into the nucleoplasm, thereby owing the participation of Srp1p in multiple rounds of nuclear import; required for accurate chromosome segregation; homolog of metazoan CAS and human CSE1L, overexpression of which is implicated in cancer progression |
| HAP2 |
YGL237C |
Nuclear transcription factor y, alpha; Transcriptional activator HAP2; Subunit of the Hap2p/3p/4p/5p CCAAT-binding complex; complex is heme-activated and glucose-repressed; complex is a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding; respiratory defect of the null mutant is functiony complemented by human NFYA |
| MTO1 |
YGL236C |
Mitochondrial protein; forms heterodimer complex with Mss1p that performs 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; required for respiration in paromomycin-resistant 15S rRNA mutants; human homolog MTO1 can complement yeast null mutant; Belongs to the MnmG family |
| YGL235W |
YGL235W |
Uncharacterized protein YGL235W; Putative protein of unknown function; potential Cdc28p substrate; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 |
| ADE5,7 |
YGL234W |
Phosphoribosylamine--glycine ligase / phosphoribosylformylglycinamidine cyclo-ligase; Bifunctional purine biosynthetic protein ADE5,7; Enzyme of the 'de novo' purine nucleotide biosynthetic pathway; contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities; In the C-terminal section; belongs to the AIR synthase family |
| SEC15 |
YGL233W |
Essential 113 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; interacts with and functions as a downstream effector of active, GTP-bound Sec4p, a Rab family GTPase |
| TAN1 |
YGL232W |
Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress |
| EMC4 |
YGL231C |
Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4 and fly CG11137; mutation is functiony complemented by human EMC4 |
| YGL230C |
YGL230C |
Uncharacterized protein YGL230C; Putative protein of unknown function; non-essential gene |
| SAP4 |
YGL229C |
SIT4-associating protein SAP4; Protein required for function of the Sit4p protein phosphatase; member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p; SAP4 has a paralog, SAP155, that arose from the whole genome duplication |
| SHE10 |
YGL228W |
Protein involved in outer spore w assembly; likely involved directly in dityrosine layer assembly; putative GPI-anchored protein; overexpression causes growth arrest;; SWAT-GFP, seamless-GFP and mCherry fusion proteins localize to the endoplasmic reticulum; SHE10 has a paralog, OSW7/YFR039C, that arose from the whole genome duplication; paralogs are redundant for spore w dityrosine assembly |
| VID30 |
YGL227W |
Vacuolar import and degradation protein 30; Central component of GID Complex, involved in FBPase degradation; interacts strongly with Gid8p to serve as a scaffold for other GID Complex subunits; contains SPRY domain and 3 domains that are also found in Gid8p - LisH, CTLH, and CRA; required for association of Vid vesicles and actin patches in vacuole import and degradation pathway; shifts the balance of nitrogen metabolism toward glutamate production; localizes to the nucleus and the cytoplasm |
| OST5 |
YGL226C-A |
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST5; Zeta subunit of the oligosaccharyltransferase complex of the ER lumen; complex catalyzes asparagine-linked glycosylation of newly synthesized proteins; Belongs to the OST5 family |
| MTC3 |
YGL226W |
Maintenance of telomere capping protein 3, mitochondrial; Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is syntheticy sick with cdc13-1 |
| YNCG0002C |
YNCG0002C |
Unknown |
| VRG4 |
YGL225W |
Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi; VRG4 has a paralog, HVG1, that arose from the whole genome duplication; Belongs to the TPT transporter family. SLC35D subfamily |
| SDT1 |
YGL224C |
Suppressor of disruption of TFIIS; Pyrimidine nucleotidase; responsible for production of nicotinamide riboside and nicotinic acid riboside; overexpression suppresses the 6-AU sensitivity of transcription elongation factor S-II, as well as resistance to other pyrimidine derivatives; SDT1 has a paralog, PHM8, that arose from the whole genome duplication |
| COG1 |
YGL223C |
Essential component of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments |
| EDC1 |
YGL222C |
Enhancer of mRNA-decapping protein 1; RNA-binding protein that activates mRNA decapping directly; binds to mRNA substrate and enhances activity of decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress; EDC1 has a paralog, EDC2, that arose from the whole genome duplication |
| NIF3 |
YGL221C |
NGG1-interacting factor 3; Protein of unknown function; similar to Listeria monocytogenes major sigma factor (rpoD gene product); the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; Belongs to the GTP cyclohydrolase I type 2/NIF3 family |
| BOL2 |
YGL220W |
BolA-like protein 2; Cytosolic protein involved in repression of iron regulon transcription; forms an iron-independent complex with Fra1p, Grx3p, and Grx4p; null mutant fails to repress the iron regulon and is sensitive to nickel; sequence similarity to human BOLA family member, BOLA2; Belongs to the BolA/IbaG family |
| MDM34 |
YGL219C |
Mitochondrial distribution and morphology protein 34; Mitochondrial component of the ERMES complex; links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth; required for mitophagy; ERMES complex is often co-localized with peroxisomes and with concentrated areas of pyruvate dehydrogenase |
| KIP3 |
YGL216W |
Kinesin-like protein KIP3; Kinesin-related antiparel sliding motor protein; involved in mitotic spindle positioning; sliding activity promotes bipolar spindle assembly and maintenance of genome stability; inhibits spindle elongation, destabilizing late anaphase spindle microtubules that polymerize beyond the midzone; Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin II subfamily |
| CLG1 |
YGL215W |
PHO85 cyclin CLG1; Cyclin-like protein that interacts with Pho85p; has sequence similarity to G1 cyclins PCL1 and PCL2 |
| SKI8 |
YGL213C |
Antiviral protein SKI8; Ski complex component and WD-repeat protein; mediates 3'-5' RNA degradation by the cytoplasmic exosome; also required for meiotic double-strand break recombination; null mutants have superkiller phenotype |
| VAM7 |
YGL212W |
Regulator of vacuolar morphogenesis; Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress |
| NCS6 |
YGL211W |
Cytoplasmic tRNA 2-thiolation protein 1; Protein required for uridine thiolation of Gln, Lys, and Glu tRNAs; required for the thiolation of uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae |
| YPT32 |
YGL210W |
GTP-binding protein YPT32/YPT11; Rab family GTPase involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; protein abundance increases in response to DNA replication stress; YPT32 has a paralog, YPT31, that arose from the whole genome duplication |
| MIG2 |
YGL209W |
Regulatory protein MIG2; Zinc finger transcriptional repressor; cooperates with Mig1p in glucose-induced gene repression; under low glucose conditions relocalizes to mitochondrion, where it interacts with Ups1p, antagonizes mitochondrial fission factor Dnm1p, indicative of a role in mitochondrial fusion or regulating morphology; regulates filamentous growth in response to glucose depletion; activated in stochastic pulses of nuclear localization in response to low glucose |
| SIP2 |
YGL208W |
One of three beta subunits of the Snf1 kinase complex; involved in the response to glucose starvation; null mutants exhibit accelerated aging; N-myristoylprotein localized to the cytoplasm and the plasma membrane; SIP2 has a paralog, GAL83, that arose from the whole genome duplication |
| SPT16 |
YGL207W |
Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; specificy required for diauxic shift-induced H2B deposition onto rDNA genes; mutations cause reduced nucleosome occupancy over highly transcribed regions; coregulates transcription with Mot1p through preinitiation complex assembly and nucleosome organization |
| CHC1 |
YGL206C |
Clathrin heavy chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; the clathrin triskelion is a trimeric molecule composed of three heavy chains that radiate from a vertex and three light chains which bind noncovalently near the vertex of the triskelion; the light chain (CLC1) is thought to regulate function |
| POX1 |
YGL205W |
Fatty-acyl coenzyme A oxidase; involved in the fatty acid beta-oxidation pathway; localized to the peroxisomal matrix |
| YNCG0003C |
YNCG0003C |
Unknown |
| YGL204C |
YGL204C |
Uncharacterized protein YGL204C; Protein of unknown function; mRNA identified as translated by ribosome profiling data; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum |
| KEX1 |
YGL203C |
Pheromone-processing carboxypeptidase KEX1; Cell death protease essential for hypochlorite-induced apoptosis; involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins |
| YNCG0004W |
YNCG0004W |
Unknown |
| ARO8 |
YGL202W |
Bifunctional 2-aminoadipate transaminase/aromatic-amino-acid:2-oxoglutarate transaminase; Aromatic/aminoadipate aminotransferase 1; Aromatic aminotransferase I; expression is regulated by general control of amino acid biosynthesis |
| MCM6 |
YGL201C |
DNA replication licensing factor MCM6; Protein involved in DNA replication; component of the Mcm2-7 hexameric helicase complex that binds chromatin as a part of the pre-replicative complex; forms a subcomplex with Mcm4p and Mcm7p |
| YNCG0005W |
YNCG0005W |
Unknown |
| EMP24 |
YGL200C |
Endosomal protein P24B; Component of the p24 complex; role in misfolded protein quality control; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles |
| YIP4 |
YGL198W |
Protein that interacts with Rab GTPases; localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport; Belongs to the YIP1 family |
| MDS3 |
YGL197W |
Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain; MDS3 has a paralog, PMD1, that arose from the whole genome duplication |
| DSD1 |
YGL196W |
D-serine dehydratase (aka D-serine ammonia-lyase); converts D-serine to pyruvate and ammonia by a reaction dependent on pyridoxal 5'-phosphate and zinc; may play a role in D-serine detoxification; L-serine is not a substrate |
| GCN1 |
YGL195W |
eIF-2-alpha kinase activator GCN1; Positive regulator of the Gcn2p kinase activity; forms a complex with Gcn20p; proposed to stimulate Gcn2p activation by an uncharged tRNA; Belongs to the GCN1 family |
| YGL194C-A |
YGL194C-A |
Uncharacterized protein YGL194C-A; Putative protein of unknown function; identified based on comparisons of the genome sequences of six Saccharomyces species; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum |
| HOS2 |
YGL194C |
Histone deacetylase and subunit of Set3 and Rpd3L complexes; required for gene activation via specific deacetylation of lysines in H3 and H4 histone tails; subunit of the Set3 complex, a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; co-localizes with Cmr1p in nuclear foci in response to DNA damage by MMS |
| RME2 |
YNCG0006C |
Unknown |
| YGL193C |
YGL193C |
Uncharacterized protein YGL193C; Haploid-specific gene repressed by a1-alpha2; turned off in sir3 null strains, absence enhances the sensitivity of rad52-327 cells to campothecin almost 100-fold |
| IME4 |
YGL192W |
N6-adenosine-methyltransferase IME4; mRNA N6-adenosine methyltransferase required for entry into meiosis; mediates N6-adenosine methylation of bulk mRNA during the induction of sporulation which includes the meiotic regulators IME1, IME2 and IME4 itself; repressed in haploids via production of antisense IME4 transcripts; transcribed in diploid cells where antisense transcription is repressed; orthologous to human METTL3 (MT-A70); Belongs to the MT-A70-like family |
| COX13 |
YGL191W |
Subunit VIa of cytochrome c oxidase; present in a subclass of cytochrome c oxidase complexes that may have a role in mimimizing generation of reactive oxygen species; not essential for cytochrome c oxidase activity but may modulate activity in response to ATP; required for assembly of Rcf2p into cytochrome c oxidase - cytochrome bc1 supercomplexes |
| CDC55 |
YGL190C |
Regulatory subunit B of protein phosphatase 2A (PP2A); Zds1p/2p-dependent localization to cytoplasm promotes mitotic entry; localization to nucleus prevents mitotic exit; required for correct nuclear division, chromosome segregation during achiasmate meiosis; maintains nucleolar sequestration of Cdc14p during early meiosis; limits formation of PP2A-Rts1p holocomplexes to ensure timely dissolution of sister chromosome cohesion; homolog of mammalian B55 |
| RPS26A |
YGL189C |
Protein component of the sm (40S) ribosomal subunit; homologous to mammalian ribosomal protein S26, no bacterial homolog; RPS26A has a paralog, RPS26B, that arose from the whole genome duplication; human homolog can partiy complement an RPS26A, RPS26B double null mutant; mutations in the human gene are associated with Diamond-Blackfan anemia |
| YGL188C-A |
YGL188C-A |
Uncharacterized protein YGL188C-A; Putative protein of unknown function |
| COX4 |
YGL187C |
Subunit IV of cytochrome c oxidase; the terminal member of the mitochondrial inner membrane electron transport chain; precursor N-terminal 25 residues are cleaved during mitochondrial import; phosphorylated; spermidine enhances translation |
| TPN1 |
YGL186C |
Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p |
| YGL185C |
YGL185C |
Putative 2-hydroxyacid dehydrogenase ygl185c; Putative protein with sequence similar to hydroxyacid dehydrogenases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm |
| STR3 |
YGL184C |
Peroxisomal cystathionine beta-lyase; converts cystathionine into homocysteine; may be redox regulated by Gto1p; involved in the release of the aromatic thiol 3-mercaptohexanol during wine fermentation; Belongs to the trans-sulfuration enzymes family |
| MND1 |
YGL183C |
Protein required for recombination and meiotic nuclear division; forms a complex with Hop2p, which is involved in chromosome pairing and repair of meiotic double-strand breaks |
| GTS1 |
YGL181W |
Protein involved in Arf3p regulation and in transcription regulation; localizes to the nucleus and to endocytic patches; contains an N-terminal Zn-finger and ArfGAP homology domain, a C-terminal glutamine-rich region, and a UBA (ubiquitin associated) domain; gts1 mutations affect budding, cell size, heat tolerance, sporulation, life span, ultradian rhythms, endocytosis; expression oscillates in a pattern similar to metabolic oscillations |
| ATG1 |
YGL180W |
Serine/threonine-protein kinase ATG1; Protein serine/threonine kinase; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; structury required for phagophore assembly site formation; during autophagy forms a complex with Atg13p and Atg17p; essential for cell cycle progression from G2/M to G1 under nitrogen starvation |
| TOS3 |
YGL179C |
Serine/threonine-protein kinase TOS3; Protein kinase; related to and functiony redundant with Elm1p and Sak1p for the phosphorylation and activation of Snf1p; functiony orthologous to LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome; TOS3 has a paralog, SAK1, that arose from the whole genome duplication |
| MPT5 |
YGL178W |
Suppressor protein MPT5; mRNA-binding protein of the PUF family; binds to specific mRNAs, often in the 3' UTR; has broad specificity and binds to more than 1000 mRNAs (16% of the transcriptome); recruits the CCR4-NOT deadenylase complex to mRNAs along with Dhh1p and Dcp1p to promote deadenylation, decapping, and decay; also interacts with the Caf20p translational initiation repressor, affecting its mRNA target specificity |
| YGL176C |
YGL176C |
Uncharacterized protein YGL176C; Putative protein of unknown function; deletion mutant is viable and has no detectable phenotype |
| SAE2 |
YGL175C |
DNA endonuclease SAE2; Endonuclease required for telomere elongation; required for telomeric 5' C-rich strand resection; involved in ds-break repair and processing hairpin DNA structures with the MRX complex; function requires sumoylation and phosphorylation; exists as inactive oligomers that are transiently released into smer active units by phosphorylation; DNA damage triggers Sae2p removal, so active Sae2p is present only transiently; sequence and functional similarity with human CtIP/RBBP8; Belongs to the COM1/SAE2/CtIP family |
| BUD13 |
YGL174W |
Pre-mRNA-splicing factor CWC26; Subunit of the RES complex; RES complex is required for nuclear pre-mRNA retention and splicing; involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern due to a specific defect in MATa1 pre-mRNA splicing which leads to haploid gene expression in diploids; Belongs to the CWC26 family |
| XRN1 |
YGL173C |
5'-3' exoribonuclease 1; Evolutionarily-conserved 5'-3' exonuclease; component of cytoplasmic processing (P) bodies involved in mRNA decay; also enters the nucleus and positively regulates transcription initiation and elongation; plays a role in microtubule-mediated processes, filamentous growth, ribosomal RNA maturation, and telomere maintenance; activated by the scavenger decapping enzyme Dcs1p |
| NUP49 |
YGL172W |
FG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of the nuclear pore complex (NPC) permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) |
| ROK1 |
YGL171W |
ATP-dependent RNA helicase ROK1; RNA-dependent ATPase; involved in pre-rRNA processing at sites A0, A1, and A2, and in control of cell cycle progression; contains two upstream open reading frames (uORFs) in 5' untranslated region which regulate translation; Belongs to the DEAD box helicase family. DDX52/ROK1 subfamily |
| SPO74 |
YGL170C |
Sporulation-specific protein 74; Component of the meiotic outer plaque of the spindle pole body; involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation |
| YNCG0007C |
YNCG0007C |
Unknown |
| SUA5 |
YGL169W |
Threonylcarbamoyl-AMP synthase; Protein involved in threonylcarbamoyl adenosine biosynthesis; Sua5p and Qri7p are necessary and sufficient for RNA t6A modification in vitro; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family; binds single-stranded telomeric DNA and null mutant has abnormal telomere length |
| HUR1 |
YGL168W |
Putative uncharacterized protein HUR1; Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene |
| PMR1 |
YGL167C |
Calcium-transporting ATPase 1; High affinity Ca2+/Mn2+ P-type ATPase; required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting, processing; D53A mutant (Mn2+ transporting) is rapamycin sensitive, Q783A mutant (Ca2+ transporting) is rapamycin resistant; Mn2+ transport into Golgi lumen required for rapamycin sensitivity; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease; human ATP2C1 can complement yeast null mutant; Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family |
| CUP2 |
YGL166W |
Transcriptional activator protein CUP2; Copper-binding transcription factor; activates transcription of the metothionein genes CUP1-1 and CUP1-2 in response to elevated copper concentrations; required for regulation of copper genes in response to DNA-damaging reagents; CUP2 has a paralog, HAA1, that arose from the whole genome duplication |
| YRB30 |
YGL164C |
Ran-specific GTPase-activating protein 30; RanGTP-binding protein; inhibits RanGAP1 (Rna1p)-mediated GTP hydrolysis of RanGTP (Gsp1p); shares similarity to proteins in other fungi but not in higher eukaryotes |
| RAD54 |
YGL163C |
DNA repair and recombination protein RAD54; DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family of DNA translocases; forms nuclear foci upon DNA replication stress |
| SUT1 |
YGL162W |
Sterol uptake protein 1; Zn(II)2Cys6 family transcription factor; positively regulates sterol uptake genes under anaerobic conditions; involved in hypoxic gene expression; represses filamentation-inducing genes during vegetative growth; positively regulates mating with SUT2 by repressing expression of genes that act as mating inhibitors; repressed by STE12; relocalizes from the nucleus to the cytoplasm upon DNA replication stress; SUT1 has a paralog, SUT2, that arose from the whole genome duplication |
| YIP5 |
YGL161C |
Protein that interacts with Rab GTPases; localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport |
| AIM14 |
YGL160W |
Probable metoreductase AIM14; NADPH oxidase localized to the perinuclear ER; produces superoxide from NADPH; overexpression causes MCA1 dependent apoptosis; likely involved in superoxide-mediated regulation of the actin cytoskeleton; member of a conserved superfamily of NADPH oxidases (NOX enzymes); has similarity to iron/copper reductases (FRE1-8), particularly Fre8p; Belongs to the ferric reductase (FRE) family. AIM14 subfamily |
| YGL159W |
YGL159W |
Uncharacterized protein YGL159W; Putative protein of unknown function; deletion mutant has no detectable phenotype |
| YNCG0008W |
YNCG0008W |
Unknown |
| RCK1 |
YGL158W |
Serine/threonine-protein kinase RCK1; Protein kinase involved in oxidative stress response; promotes pseudohyphal growth via activation of Ubp3p phosphorylation; identified as suppressor of S. pombe cell cycle checkpoint mutations; RCK1 has a paralog, RCK2, that arose from the whole genome duplication |
| ARI1 |
YGL157W |
Carbonyl reductase (nadph-dependent) ari1; NADPH-dependent aldehyde reductase; utilizes aromatic and alophatic aldehyde substrates; member of the short-chain dehydrogenase/reductase superfamily |
| AMS1 |
YGL156W |
Alpha-mannosidase; Vacuolar alpha mannosidase; involved in free oligosaccharide (fOS) degradation; delivered to the vacuole in a novel pathway separate from the secretory pathway |
| CDC43 |
YGL155W |
Beta subunit of geranylgeranyltransferase type I; subunit of the Ram2p-Cdc43p heterodimer that catalyzes the geranylgeranylation of the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis |
| LYS5 |
YGL154C |
L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase; Phosphopantetheinyl transferase involved in lysine biosynthesis; converts inactive apo-form of Lys2p (alpha-aminoadipate reductase) into catalyticy active holo-form by posttranslational addition of phosphopantetheine; Belongs to the P-Pant transferase superfamily. AcpS family |
| PEX14 |
YGL153W |
Peroxisomal membrane protein PEX14; Central component of the peroxisomal importomer complex; peroxisomal protein import machinery docking complex component; interacts with both PTS1 (Pex5p) and PTS2 (Pex7p) peroxisomal matrix protein signal recognition factors and membrane receptor Pex13p |
| NUT1 |
YGL151W |
Mediator of rna polymerase ii transcription subunit 5; Component of the RNA polymerase II mediator complex; mediator is required for transcriptional activation and also has a role in basal transcription |
| INO80 |
YGL150C |
Putative DNA helicase INO80; ATPase and nucleosome spacing factor; subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; promotes nucleosome shifts in the 3 prime direction; has a role in modulating stress gene transcription |
| YGL149W |
YGL149W |
Uncharacterized protein YGL149W; Putative protein of unknown function; conserved among S. cerevisiae strains; YGL149W is not an essential gene |
| ARO2 |
YGL148W |
Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress |
| RPL9A |
YGL147C |
Ribosomal 60S subunit protein L9A; homologous to mammalian ribosomal protein L9 and bacterial L6; RPL9A has a paralog, RPL9B, that arose from a single-locus duplication |
| RRT6 |
YGL146C |
Regulator of rDNA transcription protein 6; Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; contains two putative transmembrane spans, but no significant homology to other known proteins |
| TIP20 |
YGL145W |
Peripheral membrane protein required for COPI vesicle fusion to the ER; mediates Sey1p-independent homotypic ER fusion; prohibits back-fusion of COPII vesicles with the ER; forms a tethering complex with Sec39p and Dsl1p that interacts with ER SNAREs Sec20p and Use1p |
| ROG1 |
YGL144C |
Putative lipase ROG1; Lipase with specificity for monoacylglycerol; preferred substrate is 1-oleoylglycerol; null mutation affects lipid droplet morphology and overexpression causes increased accumulation of reactive oxygen species |
| MRF1 |
YGL143C |
Mitochondrial translation release factor; involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability |
| GPI10 |
YGL142C |
GPI mannosyltransferase 3; Integral membrane protein involved in GPI anchor synthesis; putative alpha 1,2 mannosyltransferase required for addition of the third mannose onto the glycosylphosphatidylinositol (GPI) core structure; human PIG-Bp is a functional homolog |
| HUL5 |
YGL141W |
Probable E3 ubiquitin-protein ligase HUL5; Multiubiquitin chain assembly factor (E4); proteasome processivity factor that elongates polyUb chains on substrates, opposing Ubp6p, a branched polyubiquitin protease; required for retrograde transport of misfolded proteins during ERAD; required for ubiquitination of a subset of cytosolic misfolded proteins upon heat shock |
| YGL140C |
YGL140C |
Uncharacterized membrane protein YGL140C; Putative protein of unknown function; non-essential gene; contains multiple predicted transmembrane domains |
| FLC3 |
YGL139W |
Putative flavin carrier protein 3; Putative FAD transporter, similar to Flc1p and Flc2p; localized to the ER; FLC3 has a paralog, FLC1, that arose from the whole genome duplication |
| YGL138C |
YGL138C |
Uncharacterized protein YGL138C; Putative protein of unknown function; has no significant sequence similarity to any known protein |
| SEC27 |
YGL137W |
Coatomer subunit beta; Essential beta'-coat protein of the COPI coatomer; involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP |
| MRM2 |
YGL136C |
Mitochondrial 2' O-ribose methyltransferase; required for methylation of U(2791) in 21S rRNA; MRM2 deletion confers thermosensitive respiration and loss of mitochondrial DNA; has similarity to Spb1p and Trm7p, and to E. coli FtsJ/RrmJ; Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family |
| RPL1A |
YPL220W |
Ribosomal 60S subunit protein L1A; N-terminy acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1A has a paralog, RPL1B, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal |
| PCL10 |
YGL134W |
Pho85p cyclin; recruits, activates, and targets Pho85p cyclin-dependent protein kinase to its substrate; PCL10 has a paralog, PCL8, that arose from the whole genome duplication |
| ITC1 |
YGL133W |
Imitation switch two complex protein 1; Subunit of ATP-dependent Isw2p-Itc1p chromatin remodeling complex; required for repression of a-specific genes, repression of early meiotic genes during mitotic growth, and repression of INO1; similar to mammalian Acf1p, the regulatory subunit of the mammalian ATP-utilizing chromatin assembly and modifying factor (ACF) complex; ITC1 has a paralog, YPL216W, that arose from the whole genome duplication |
| SNT2 |
YGL131C |
Subunit of Snt2C complex, RING finger ubiquitin ligase (E3); physicy associates with Ecm5p and Rpd3p; along with Ecm5p, recruits Rpd3p to sm number of promoters; colocalizes with Ecm5p, independently of Rpd3p, to promoters of stress response genes upon oxidative stress; involved in ubiquitination, degradation of excess histones; interacts with Ubc4p; role in regulating genes encoding amine transporters; relocalizes from nucleus to cytoplasm upon DNA replication stress |
| CEG1 |
YGL130W |
mRNA-capping enzyme subunit alpha; Guanylyltransferase involved in mRNA 5' capping; subunit of mRNA capping enzyme, which is a heterotetramer composed of two molecules of Ceg1p and a homodimer of Cet1p, the mRNA 5'-triphosphatase subunit; nuclear import of Ceg1p requires interaction with Cet1p; mammalian capping enzyme is a single bifunctional polypeptide; human homolog RNGTT can complement yeast ceg1 null mutant |
| RSM23 |
YGL129C |
Mitochondrial ribosomal protein of the sm subunit; has similarity to mammalian apoptosis mediator proteins; null mutation prevents induction of apoptosis by overproduction of metacaspase Mca1p |
| CWC23 |
YGL128C |
Pre-mRNA-splicing factor CWC23; Component of a complex containing Cef1p; putatively involved in pre-mRNA splicing; has similarity to E. coli DnaJ and other DnaJ-like proteins and to S. pombe Cwf23p |
| SOH1 |
YGL127C |
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits |
| SCS3 |
YGL126W |
FIT family protein SCS3; Protein required for inositol prototrophy; required for normal ER membrane biosynthesis; ortholog of the FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; disputed role in the synthesis of inositol phospholipids from inositol |
| MET13 |
YGL125W |
Methylenetetrahydrofolate reductase (nad(p)h) met13; Major isozyme of methylenetetrahydrofolate reductase; catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the methionine biosynthesis pathway |
| MON1 |
YGL124C |
Vacuolar fusion protein MON1; Subunit of a heterodimeric guanine nucleotide exchange factor (GEF); subunit of the Mon1-Ccz1 GEF complex which stimulates nucleotide exchange and activation of Ypt7p, a Rab family GTPase involved in membrane tethering and fusion events at the late endosome and vacuole; GEF activity is stimulated by membrane association and anionic phospholipids; role in localizing Ypt7p to the vacuolar membrane; required for autophagy, the CVT pathway and mitophagy; potential Cdc28 substrate; Belongs to the MON1/SAND family |
| RPS2 |
YGL123W |
Protein component of the sm (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 |
| NAB2 |
YGL122C |
Nuclear polyadenylated RNA-binding protein; required for nuclear mRNA export and poly(A) tail length control; stimulates RNA polymerase III transcription by enhancing TFIIIB binding to promoters; protects mRNA against decay by the nuclear exosome in a poly(A)-tail-dependent manner; involved in forming export-competent mRNPs in the nucleus; autoregulates mRNA levels; NLS binds Kap104p; protein abundance increases under DNA replication stress; related to human hnRNPs; Belongs to the NAB2 family |
| GPG1 |
YGL121C |
Proposed gamma subunit of the heterotrimeric G protein; interacts with the receptor Gpr1p; involved in regulation of pseudohyphal growth; requires Gpb1p or Gpb2p to interact with Gpa2p; overproduction causes prion curing |
| PRP43 |
YGL120C |
Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP43; RNA helicase in the DEAH-box family; functions in both RNA polymerase I and polymerase II transcript metabolism; catalyzes removal of U2, U5, and U6 snRNPs from the postsplicing lariat-intron ribonucleoprotein complex; required for efficient biogenesis of both sm- and large-subunit rRNAs; acts with Sqs1p to promote 20S to 18S rRNA processing catalyzed by endonuclease Nob1p; Belongs to the DEAD box helicase family. DEAH subfamily. DDX15/PRP43 sub-subfamily |
| COQ8 |
YGL119W |
Atypical kinase COQ8, mitochondrial; ATPase required for ubiquinone biosynthesis and respiratory growth; maintains levels of CoQ biosynthetic proteins; binds to CoQ biosynthesis intermediates; UbiB protein kinase-like family member that lacks canonical protein kinase activity; similar to prokaryotic proteins involved in ubiquinone biosynthesis; human homolog ADCK3 complements a coq8 null, is associated with CoQ and respiratory-chain deficiencies, and is mutated in autosomal-recessive cerebellar ataxia type 2 |
| YNCG0009C |
YNCG0009C |
Unknown |
| YGL118C |
YGL118C |
Uncharacterized protein YGL118C; Putative protein of unknown function; conserved among S. cerevisiae strains; YGL118C is not an essential gene |
| YGL117W |
YGL117W |
Uncharacterized protein YGL117W; Putative protein of unknown function |
| CDC20 |
YGL116W |
Ubiquitin-protein transferase activating protein cdc20; Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress; Belongs to the WD repeat CDC20/Fizzy family |
| SNF4 |
YGL115W |
Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress |
| YGL114W |
YGL114W |
Putative oligopeptide transporter ygl114w; Putative protein of unknown function; predicted member of the oligopeptide transporter (OPT) family of membrane transporters |
| SLD3 |
YGL113W |
Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress; homologous to the human Treslin/Ticrr protein |
| TAF6 |
YGL112C |
Subunit (60 kDa) of TFIID and SAGA complexes; involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4; relocalizes to the cytosol in response to hypoxia |
| NSA1 |
YGL111W |
Ribosome biogenesis protein NSA1; Constituent of 66S pre-ribosomal particles; involved in 60S ribosomal subunit biogenesis |
| CUE3 |
YGL110C |
Cue domain-containing protein 3; Protein of unknown function; has a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination |
| YGL108C |
YGL108C |
Uncharacterized protein YGL108C; Protein of unknown function, predicted to be palmitoylated; SWAT-GFP, seamless-GFP and mCherry C-terminal fusion proteins localize to the cytosol, while N-terminal GFP fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress; To S.pombe new13 |
| RMD9 |
YGL107C |
Mitochondrial protein required for respiratory growth; mutant phenotype and genetic interactions suggest a role in delivering mt mRNAs to ribosomes; located on matrix face of the inner membrane and loosely associated with mitoribosomes; RMD9 has a paralog, YBR238C, that arose from the whole genome duplication |
| MLC1 |
YGL106W |
Essential light chain for Myo1p; light chain for Myo2p; stabilizes Myo2p by binding to the neck region; interacts with Myo1p, Iqg1p, and Myo2p to coordinate formation and contraction of the actomyosin ring with targeted membrane deposition |
| ARC1 |
YGL105W |
tRNA-aminoacylation cofactor ARC1; Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases; involved in tRNA delivery, stimulating catalysis, and ensuring localization; also binds quadruplex nucleic acids; protein abundance increases in response to DNA replication stress; methionyl-tRNA synthetase is Mes1p; glutamyl-tRNA synthetase is Gus1p |
| VPS73 |
YGL104C |
Mitochondrial protein; mutation affects vacuolar protein sorting; putative transporter; member of the sugar porter family; VPS73 has a paralog, YBR241C, that arose from the whole genome duplication |
| RPL28 |
YGL103W |
Ribosomal 60S subunit protein L28; homologous to mammalian ribosomal protein L27A and bacterial L15; may have peptidyl transferase activity; can mutate to cycloheximide resistance |
| YGK1 |
YGL101W |
HD domain-containing protein YGL101W; Protein of unknown function; non-essential gene; interacts with the DNA helicase Hpr5p; YGL101W has a paralog, YBR242W, that arose from the whole genome duplication |
| SEH1 |
YGL100W |
Nucleoporin SEH1; Subunit of the Nup84 nuclear pore and SEACAT subcomplexes; involved in nucleocytoplasmic transport and NPC biogenesis in the nuclear pore subcomplex; subunit of SEACAT, a subcomplex of the SEA complex that inhibits the TORC1 inhibitory role of SEACIT (Iml1p-Npr2p-Npr3p), a GAP for Gtr1p in response to amino acid limitation, thereby resulting in activation of TORC1 signaling; SEA is a coatomer-related complex that associates dynamicy with the vacuole; human SEH1 homolog |
| LSG1 |
YGL099W |
Large subunit GTPase 1; Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm; Belongs to the TRAFAC class YlqF/YawG GTPase family. LSG1 subfamily |
| SNR82 |
YNCG0010W |
Unknown |
| USE1 |
YGL098W |
Essential SNARE protein localized to the ER; involved in retrograde traffic from the Golgi to the ER and Sey1p-independent homotypic ER fusion; required for efficient nuclear fusion during mating; forms a complex with the SNAREs Sec22p, Sec20p and Ufe1p |
| YNCG0011W |
YNCG0011W |
Unknown |
| SRM1 |
YGL097W |
Nucleotide exchange factor for Gsp1p; localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentiy phosphorylated by Cdc28p; human homolog of the RAN GEF, RCC1, can complement a temperature sensitive point mutant |
| TOS8 |
YGL096W |
Homeobox protein TOS8; Homeodomain-containing protein and putative transcription factor; found associated with chromatin; target of SBF transcription factor; induced during meiosis and under cell-damaging conditions; TOS8 has a paralog, CUP9, that arose from the whole genome duplication |
| SOE1 |
YNCG0012W |
Unknown |
| VPS45 |
YGL095C |
Protein of the Sec1p/Munc-18 family; essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment |
| PAN2 |
YGL094C |
Catalytic subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex; complex acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes; Belongs to the peptidase C19 family. PAN2 subfamily |
| SPC105 |
YGL093W |
Spindle pole body component SPC105; Subunit of a kinetochore-microtubule binding complex; complex bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components; complex also includes Kre28p; modified by sumoylation |
| NUP145 |
YGL092W |
Nucleoporin NUP145; Essential protein with distinct roles in two nuclear pore subcomplexes; catalyzes its own proteolytic cleavage in vivo to generate a C-terminal fragment that is a structural component of the Nup84p subcomplex (with roles in NPC biogenesis and localization of genes to the nuclear periphery), and an N-terminal fragment that is one of several FG-nucleoporins within the NPC central core directly responsible for nucleocytoplasmic transport; homologous to human NUP98 |
| NBP35 |
YGL091C |
Cytosolic Fe-S cluster assembly factor NBP35; Essential cytoplasmic iron-sulfur cluster binding protein; forms a complex with Cfd1p that is involved in iron-sulfur protein assembly in the cytosol; similar to P-loop NTPases |
| LIF1 |
YGL090W |
Ligase-interacting factor 1; Component of the DNA ligase IV complex; this complex mediates nonhomologous end joining in DNA double-strand break repair; physicy interacts with Dnl4p and Nej1p; homologous to mammalian XRCC4 protein |
| MF(ALPHA)2 |
YGL089C |
Mating pheromone alpha-factor, made by alpha cells; interacts with mating type a cells to induce cell cycle arrest and other responses leading to mating; also encoded by MF(ALPHA)1, which is more highly expressed; binds copper(II) ions |
| YGL088W |
YGL088W |
Uncharacterized protein YGL088W; Putative protein of unknown function; conserved across S. cerevisiae strains; partiy overlaps snR10, a snoRNA required for preRNA processing |
| SNR10 |
YNCG0013W |
Unknown |
| MMS2 |
YGL087C |
Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress |
| MAD1 |
YGL086W |
Coiled-coil protein involved in spindle-assembly checkpoint; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon spindle assembly checkpoint arrest; phosphorylated by Mps1p upon checkpoint activation which leads to inhibition of anaphase promoting complex activity; forms a complex with Mad2p; gene dosage imbalance between MAD1 and MAD2 leads to chromosome instability; Belongs to the MAD1 family |
| LCL3 |
YGL085W |
Probable endonuclease LCL3; Putative protein of unknown function; mutant has long chronological lifespan; has homology to Staphylococcus aureus nuclease; GFP-fusion protein localizes to mitochondria; is induced in response to the DNA-damaging agent MMS |
| GUP1 |
YGL084C |
Glycerol uptake protein 1; Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; role in misfolded protein quality control; proposed to be involved in glycerol transport; homolog of the mammalian Hedgehog pathway modulator HHATL; GUP1 has a paralog, GUP2, that arose from the whole genome duplication |
| SCY1 |
YGL083W |
Protein kinase-like protein SCY1; Putative kinase; suppressor of GTPase mutant; similar to bovine rhodopsin kinase; may have a role in intracellular sterol transport |
| YGL082W |
YGL082W |
Ubiquitin carboxyl-terminal hydrolase mindy-1/2; Uncharacterized protein YGL082W; Putative protein of unknown function; predicted prenylation/proteolysis target of Afc1p and Rce1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; not an essential gene; YGL082W has a paralog, YPL191C, that arose from the whole genome duplication |
| YGL081W |
YGL081W |
Uncharacterized protein YGL081W; Putative protein of unknown function; non-essential gene; interacts geneticy with CHS5, a gene involved in chitin biosynthesis |
| MPC1 |
YGL080W |
Highly conserved subunit of mitochondrial pyruvate carrier (MPC); MPC is a mitochondrial inner membrane complex that mediates pyruvate uptake and comprises Mpc1p and Mpc2p during fermentative growth, or Mcp1p and Mpc3p during respiratory growth; null mutant displays slow growth that is complemented by expression of human or mouse ortholog; mutation in human ortholog MPC1 is associated with lactic acidosis and hyperpyruvatemia |
| KXD1 |
YGL079W |
Subunit of the BLOC-1 complex involved in endosomal maturation; null mutant is sensitive to drug inducing secretion of vacuolar cargo; GFP-fusion protein localizes to the endosome; Belongs to the KXD1 family |
| DBP3 |
YGL078C |
ATP-dependent RNA helicase DBP3; RNA-Dependent ATPase, member of DExD/H-box family; involved in cleavage of site A3 within the ITS1 spacer during rRNA processing; not essential for growth, but deletion causes severe slow-growth phenotype |
| HNM1 |
YGL077C |
Plasma membrane transporter for choline, ethanolamine, and carnitine; involved in the uptake of nitrogen mustard and the uptake of glycine betaine during hypersaline stress; co-regulated with phospholipid biosynthetic genes and negatively regulated by choline and myo-inositol |
| RPL7A |
YGL076C |
Ribosomal 60S subunit protein L7A; required for processing of 27SA3 pre-rRNA to 27SB pre-rRNA during assembly of large ribosomal subunit; depletion leads to a turnover of pre-rRNA; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); binds to Domain II of 25S and 5.8S rRNAs; homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7A has a paralog, RPL7B, that arose from the whole genome duplication |
| SNR39 |
YNCG0014C |
Unknown |
| SNR39B |
YNCG0015C |
Unknown |
| MPS2 |
YGL075C |
Monopolar spindle protein 2; Essential membrane protein localized at nuclear envelope and SPBs; required for insertion of the newly duplicated spindle pole body into the nuclear envelope; potentiy phosphorylated by Cdc28p; MPS2 has a paralog, CSM4, that arose from the whole genome duplication |
| HSF1 |
YGL073W |
Trimeric heat shock transcription factor; activates multiple genes in response to highly diverse stresses; recognizes variable heat shock elements (HSEs) consisting of inverted NGAAN repeats; monitors translational status of cell through an RQC (Ribosomal Quality Control)-mediated translation-stress signal; involved in diauxic shift; posttranslationy regulated; human homolog HSF1 with linker region mutations can complement yeast hsf1 mutant; Belongs to the HSF family |
| AFT1 |
YGL071W |
Iron-regulated transcriptional activator AFT1; Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions localization is regulated by Grx3p, Grx4p, and Fra2p, and promoter binding is negatively regulated via Grx3p-Grx4p binding; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress |
| RPB9 |
YGL070C |
RNA polymerase II subunit B12.6; contacts DNA; mutations affect transcription start site selection and fidelity of transcription |
| MNP1 |
YGL068W |
Mitochondrial ribosomal protein of the large subunit; has similarity to E. coli L7/L12 and human MRPL7 ribosomal proteins; associates with the mitochondrial nucleoid; required for normal respiratory growth |
| NPY1 |
YGL067W |
NADH diphosphatase (pyrophosphatase); hydrolyzes the pyrophosphate linkage in NADH and related nucleotides; localizes to peroxisomes; nudix hydrolase family member |
| SGF73 |
YGL066W |
SAGA-associated factor 73; Subunit of DUBm module of SAGA and SLIK; has roles in anchoring deubiquitination module (DUBm) into SAGA and SLIK complexes, maintaining organization and ubiquitin-binding conformation of Ubp8p, thereby contributing to over DUBm activity; involved in preinitiation complex assembly at promoters; relocalizes to cytosol under hypoxia; human homolog ATXN7 implicated in spinocerebellar ataxia, and can complement yeast null mutant |
| ALG2 |
YGL065C |
GDP-Man:Man(1)GlcNAc(2)-PP-dolichol alpha-1,3-mannosyltransferase; Alpha-1,3/1,6-mannosyltransferase ALG2; Mannosyltransferase in the N-linked glycosylation pathway; catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol; human ALG2 complements the temperature sensitivity and dolichol-linked oligosaccharide biosynthesis defect of the alg2-1 mutant, but mutant form from a patient with CDG-Ii fails to complement; Belongs to the glycosyltransfe [...] |
| MRH4 |
YGL064C |
Mitochondrial ATP-dependent RNA helicase of the DEAD-box family; required for assembly of the large subunit of mitochondrial ribosomes; binds to the large subunit rRNA, 21S_rRNA; localizes to the matrix face of the mitochondrial inner membrane and associates with the large subunit precursor and with mature ribosomes |
| PUS2 |
YGL063W |
Mitochondrial tRNA:pseudouridine synthase; acts at positions 27 and 28, but not at position 72; efficiently and rapidly targeted to mitochondria, specificy dedicated to mitochondrial tRNA modification; mutation also affects pseudouridylation of some nuclear-encoded mRNAs; PUS2 has a paralog, PUS1, that arose from the whole genome duplication |
| PYC1 |
YGL062W |
Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentiy regulated than isoform Pyc2p; mutations in the human homolog are associated with lactic acidosis; PYC1 has a paralog, PYC2, that arose from the whole genome duplication |
| DUO1 |
YGL061C |
Essential subunit of the Dam1 complex (aka DASH complex); cooperates with Dam1p to connect the DASH complex with microtubules (MT); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis |
| YBP2 |
YGL060W |
YAP1-binding protein 2; Central kinetochore associated protein; mediates mitotic progression; interacts with several central kinetochore proteins and centromeric histone Cse4p; role in resistance to oxidative stress; similar to Slk19p; YBP2 has a paralog, YBP1, that arose from the whole genome duplication |
| PKP2 |
YGL059W |
Mitochondrial protein kinase; negatively regulates activity of the pyruvate dehydrogenase complex by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp1p and phosphatases Ptc5p and Ptc6p; relocalizes from mitochondrion to cytoplasm upon DNA replication stress |
| RAD6 |
YGL058W |
E2 ubiquitin-conjugating protein RAD6; Ubiquitin-conjugating enzyme (E2); involved in postreplication repair as a heterodimer with Rad18p, regulation of K63 polyubiquitination in response to oxidative stress, DSBR and checkpoint control as a heterodimer with Bre1p, ubiquitin-mediated N-end rule protein degradation as a heterodimer with Ubr1p, ERAD with Ubr1p in the absence of canonical ER membrane ligases, and Rpn4p turnover as part of proteasome homeostasis, in complex with Ubr2p and Mub1p |
| GEP7 |
YGL057C |
Genetic interactor of prohibitin 7, mitochondrial; Protein of unknown function; null mutant exhibits a respiratory growth defect and synthetic interactions with prohibitin (phb1) and gem1; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies |
| SDS23 |
YGL056C |
Protein involved in cell separation during budding; one of two S. cerevisiae homologs (Sds23p and Sds24p) of the S. pombe Sds23 protein, which is implicated in APC/cyclosome regulation; SDS23 has a paralog, SDS24, that arose from the whole genome duplication |
| OLE1 |
YGL055W |
Stearoyl-coa desaturase (delta-9 desaturase); Acyl-CoA desaturase 1; Delta(9) fatty acid desaturase; required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria |
| ERV14 |
YGL054C |
ER-derived vesicles protein ERV14; COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p and Nha1p antiporter to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication |
| YNCG0016C |
YNCG0016C |
Unknown |
| YNCG0017W |
YNCG0017W |
Unknown |
| TYW3 |
YGL050W |
tRNA wybutosine-synthesizing protein 3; tRNA methyltransferase required for synthesis of wybutosine; a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions; Belongs to the TYW3 family |
| TIF4632 |
YGL049C |
Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); associates with the poly(A)-binding protein Pab1p, also interacts with eIF4A (Tif1p); TIF4632 has a paralog, TIF4631, that arose from the whole genome duplication |
| RPT6 |
YGL048C |
ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress |
| ALG13 |
YGL047W |
UDP-N-acetylglucosamine transferase subunit ALG13; Catalytic component of UDP-GlcNAc transferase; required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases; protein abundance increases in response to DNA replication stress; both human homologs ALG13 and ALG14 are required to complement yeast alg13 mutant |
| YNCG0018C |
YNCG0018C |
Unknown |
| RIM8 |
YGL045W |
pH-response regulator protein palF/RIM8; Protein involved in proteolytic activation of Rim101p; part of response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family |
| RNA15 |
YGL044C |
mRNA 3'-end-processing protein RNA15; Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping and maintenance of genome stability |
| DST1 |
YGL043W |
General transcription elongation factor TFIIS; enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts sted at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress; Belongs to the TFS-II family |
| YGL041C-B |
YGL041C-B |
Uncharacterized protein YGL041C-B; Putative protein of unknown function; identified by fungal homology and RT-PCR; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum |
| DPC13 |
YGL041W-A |
Uncharacterized protein YGL041W-A, mitochondrial; Putative protein of unknown function; conserved in fungi; identified by expression profiling and mass spectrometry |
| HEM2 |
YGL040C |
Delta-aminolevulinic acid dehydratase; Aminolevulinate dehydratase; a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus; human homolog ALAD can complement yeast hem2 mutant |
| SUP54 |
YNCG0019W |
Unknown |
| YGL039W |
YGL039W |
Putative uncharacterized oxidoreductase YGL039W; Aldehyde reductase; reduces aliphatic aldehyde substrates using NADH as cofactor; shown to reduce carbonyl compounds to chiral alcohols |
| OCH1 |
YGL038C |
Initiation-specific alpha-1,6-mannosyltransferase; Mannosyltransferase of the cis-Golgi apparatus; initiates the polymannose outer chain elongation of N-linked oligosaccharides of glycoproteins; Belongs to the glycosyltransferase 32 family |
| PNC1 |
YGL037C |
Nicotinamidase that converts nicotinamide to nicotinic acid; part of the NAD(+) salvage pathway; required for life span extension by calorie restriction; lacks a peroxisomal targeting signal but is imported into peroxisomes via binding to Gpd1p; PNC1 expression responds to known stimuli that extend replicative life span; protein increases in abundance and relative distribution to cytoplasmic foci decreases upon DNA replication stress |
| YGL036W |
YGL036W |
Uncharacterized protein YGL036W; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YGL036W is not an essential gene |
| MIG1 |
YGL035C |
Regulatory protein MIG1; Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase; regulates filamentous growth along with Mig2p in response to glucose depletion; activated in stochastic pulses of nuclear localization, shuttling between cytosol and nucleus depending on external glucose levels and its phosphorylation state; Belongs to the creA/MIG C2H2-type zinc-finger protein family |
| YGL034C |
YGL034C |
Uncharacterized protein YGL034C; Putative protein of unknown function; conserved among S. cerevisiae strains; YGL034C is not an essential gene |
| HOP2 |
YGL033W |
26S proteasome regulatory subunit, ATPase 3, interacting protein; Homologous-pairing protein 2; Meiosis-specific protein that localizes to chromosomes; prevents synapsis between nonhomologous chromosomes and ensures synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair; heterodimer of Hop2p-Mnd1p stimulates the Dmc1p-mediated strand invasion |
| AGA2 |
YGL032C |
A-agglutinin-binding subunit; Adhesion subunit of a-agglutinin of a-cells; C-terminal sequence acts as a ligand for alpha-agglutinin (Sag1p) during agglutination, modified with O-linked oligomannosyl chains, linked to anchorage subunit Aga1p via two disulfide bonds |
| RPL24A |
YGL031C |
Ribosomal 60S subunit protein L24A; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24A has a paralog, RPL24B, that arose from the whole genome duplication |
| RPL30 |
YGL030W |
Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog |
| CGR1 |
YGL029W |
rRNA-processing protein CGR1; Protein involved in nucleolar integrity and processing of pre-rRNA; has a role in processing rRNA for the 60S ribosome subunit; transcript is induced in response to cytotoxic stress but not genotoxic stress; relocalizes from nucleus to nucleolus upon DNA replication stress |
| YNCG0020C |
YNCG0020C |
Unknown |
| SCW11 |
YGL028C |
Probable family 17 glucosidase SCW11; Cell w protein with similarity to glucanases; may play a role in conjugation during mating based on its regulation by Ste12p |
| CWH41 |
YGL027C |
Processing alpha glucosidase I; ER type II integral membrane N-glycoprotein involved in assembly of cell w beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress; Belongs to the glycosyl hydrolase 63 family |
| TRP5 |
YGL026C |
Tryptophan synthase; catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis; In the N-terminal section; belongs to the TrpA family |
| PGD1 |
YGL025C |
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor |
| PIB2 |
YGL023C |
Protein of unknown function; contains FYVE domain; similar to Fab1 and Vps27 |
| STT3 |
YGL022W |
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3; Subunit of the oligosaccharyltransferase complex of the ER lumen; complex catalyzes asparagine-linked glycosylation of newly synthesized proteins; forms a subcomplex with Ost3p and Ost4p and is directly involved in catalysis |
| ALK1 |
YGL021W |
Serine/threonine-protein kinase Haspin homolog ALK1; Protein kinase; along with its paralog, ALK2, required for proper spindle positioning and nuclear segregation following mitotic arrest, proper organization of cell polarity factors in mitosis, proper localization of formins and polarity factors, and survival in cells that activate spindle assembly checkpoint; phosphorylated in response to DNA damage; ALK1 has a paralog, ALK2, that arose from the whole genome duplication; similar to mammalian haspins |
| GET1 |
YGL020C |
Golgi to ER traffic protein 1; Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance |
| CKB1 |
YGL019W |
Beta regulatory subunit of casein kinase 2 (CK2); a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and RNA polymerases |
| JAC1 |
YGL018C |
J-type co-chaperone JAC1, mitochondrial; Specialized J-protein that functions in Fe-S cluster biogenesis; functions with Hsp70 in Fe-S cluster biogenesis in mitochondria; involved in iron metabolism; contains a J domain typical to J-type chaperones; localizes to the mitochondrial matrix |
| ATE1 |
YGL017W |
Arginyl-tRNA--protein transferase 1; Arginyl-tRNA-protein transferase; catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway; Belongs to the R-transferase family |
| KAP122 |
YGL016W |
Importin beta-like protein KAP122; Karyopherin beta; responsible for import of the Toa1p-Toa2p complex into the nucleus; binds to nucleoporins Nup1p and Nup2p; may play a role in regulation of pleiotropic drug resistance |
| BIL2 |
YGL015C |
Uncharacterized protein YGL015C; Putative protein of unknown function; null mutants accumulate cargo in the Golgi |
| PUF4 |
YGL014W |
Pumilio homology domain family member 4; Member of the PUF protein family; PUF family is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentiy binds mRNAs encoding nucleolar ribosomal RNA-processing factors |
| PDR1 |
YGL013C |
Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; relocalizes to the cytosol in response to hypoxia; PDR1 has a paralog, PDR3, that arose from the whole genome duplication |
| ERG4 |
YGL012W |
C-24(28) sterol reductase; catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol; Belongs to the ERG4/ERG24 family |
| SCL1 |
YGL011C |
Alpha 1 subunit of the 20S proteasome; involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria; Belongs to the peptidase T1A family |
| MPO1 |
YGL010W |
Uncharacterized endoplasmic reticulum membrane protein YGL010W; Protein involved in metabolism of phytosphingosine; not an essential gene |
| LEU1 |
YGL009C |
3-isopropylmalate dehydratase; Isopropylmalate isomerase; catalyzes the second step in the leucine biosynthesis pathway; Belongs to the aconitase/IPM isomerase family |
| PMA1 |
YGL008C |
Plasma membrane P2-type H+-ATPase; pumps protons out of cell; major regulator of cytoplasmic pH and plasma membrane potential; long-lived protein asymmetricy distributed at plasma membrane between mother cells and buds; accumulates at high levels in mother cells during aging, buds emerge with very low levels of Pma1p, newborn cells have low levels of Pma1p; Hsp30p plays a role in Pma1p regulation; interactions with Std1p appear to propagate [GAR+] |
| YGL007C-A |
YGL007C-A |
Uncharacterized protein YGL007C-A; Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; deletion exhibits slow-growth phenotype; computationy predicted to have a role in cell budding |
| BRP1 |
YGL007W |
Uncharacterized protein BRP1; Putative protein of unknown function; conserved among S. cerevisiae strains; located in the upstream region of PMA1; deletion leads to polyamine resistance due to downregulation of PMA1 |
| YGL006W-A |
YGL006W-A |
Uncharacterized protein YGL006W-A; Putative protein of unknown function; identified by SAGE |
| PMC1 |
YGL006W |
Vacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ions; prevents growth inhibition by activation of calcineurin in the presence of elevated concentrations of calcium; similar to mammalian PMCA1a; Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family |
| COG7 |
YGL005C |
Conserved oligomeric golgi complex subunit 7; Component of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments |
| RPN14 |
YGL004C |
26S proteasome regulatory subunit RPN14; Evolutionarily conserved 19S regulatory particle assembly-chaperone; proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasome regulatory particle (RP); null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; interacts with Rpt5p |
| CDH1 |
YGL003C |
Activator of anaphase-promoting complex/cyclosome (APC/C); antagonist of the spindle assembly checkpoint; directs ubiquitination of cyclins resulting in mitotic exit; targets the APC/C to specific substrates including: Cdc20p, Ase1p, Cin8p, Fin1p and Clb5p; partiy active in metaphase, and fully active in anaphase; cell-cycle regulated; Belongs to the WD repeat CDC20/Fizzy family |
| ERP6 |
YGL002W |
Protein ERP6; Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; ERP6 has a paralog, ERP1, that arose from the whole genome duplication |
| ERG26 |
YGL001C |
Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating; C-3 sterol dehydrogenase; catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; human homolog NSDHL implicated in CK syndrome, and can complement yeast null mutant; molecular target of natural product and antifungal compound FR171456; Belongs to the 3-beta-HSD family |
| EFM5 |
YGR001C |
Protein-lysine N-methyltransferase EFM5; S-adenosylmethionine-dependent lysine methyltransferase; involved in the trimethylation of eEF1A (Tef1p/Tef2p) at lysine 79; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; required for replication of Brome mosaic virus in budding yeast; expresses a circular RNA; originy misclassified as a N-6-adenine specific DNA methyltransferase based on sequence similarity; both Efm5p and human ortholog N6AMT2 can methylate eEF1a from either species in vitro |
| SWC4 |
YGR002C |
SWR1-complex protein 4; Component of the Swr1p complex that incorporates Htz1p into chromatin; component of the NuA4 histone acetyltransferase complex |
| CUL3 |
YGR003W |
Cullin-3; Ubiquitin-protein ligase; forms a complex with Elc1p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; cullin family member with similarity to Cdc53p and human CUL3; Belongs to the cullin family |
| PEX31 |
YGR004W |
Peroxisomal integral membrane protein; involved in negative regulation of peroxisome size; partiy functiony redundant with Pex30p and Pex32p; probably acts at a step downstream of steps mediated by Pex28p and Pex29p; PEX31 has a paralog, PEX30, that arose from the whole genome duplication; Belongs to the PEX28-32 family. PEX30/31 subfamily |
| TFG2 |
YGR005C |
TFIIF (Transcription Factor II) middle subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP30; Belongs to the TFIIF beta subunit family |
| PRP18 |
YGR006W |
Pre-mRNA-splicing factor 18; Splicing factor and component of snRNP U5; factor involved in the positioning of the 3' splice site during the second catalytic step of splicing; interacts with Slu7p |
| ECT1 |
YGR007W |
Ethanolamine-phosphate cytidylyltransferase; catalyzes the second step of phosphatidylethanolamine biosynthesis; involved in the maintenance of plasma membrane; similar to mammalian CTP: phosphocholine cytidylyl-transferases; inability of the null mutant to synthesize phosphatidylethanolamine and phosphatidylcholine from ethanolamine is functiony complemented by human PCYT2 |
| STF2 |
YGR008C |
ATPase-stabilizing factor 15 kDa protein; Protein involved in resistance to desiccation stress; Stf2p exhibits antioxidant properties, and its overexpression prevents ROS accumulation and apoptosis; binds to F0 sector of mitochondrial F1F0 ATPase in vitro and may modulate the inhibitory action of Inh1p and Stf1p; protein abundance increases in response to DNA replication stress; STF2 has a paralog, TMA10, that arose from the whole genome duplication |
| SEC9 |
YGR009C |
t-SNARE protein required for secretory vesicle-plasma membrane fusion; similar to but not functiony redundant with Spo20p; interacts non-exocyst bound Sec6p; SNAP-25 homolog |
| NMA2 |
YGR010W |
Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in de novo and salvage synthesis of NAD(+); homolog of human NMNAT; NMA2 has a paralog, NMA1, that arose from the whole genome duplication |
| MCY1 |
YGR012W |
Putative cysteine synthase; localized to the mitochondrial outer membrane |
| SNU71 |
YGR013W |
U1 sm nuclear ribonucleoprotein component SNU71; Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; Belongs to the SNU71 family |
| MSB2 |
YGR014W |
Signaling mucin MSB2; Mucin family member involved in various signaling pathways; functions as osmosensor in the Sho1p-mediated HOG pathway; functions in Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; processed into secreted and cell-associated forms by aspartyl protease Yps1p; potential Cdc28p substrate |
| EAT1 |
YGR015C |
Uncharacterized abhydrolase domain-containing protein YGR015C; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion |
| YGR016W |
YGR016W |
Uncharacterized membrane protein YGR016W; Putative protein of unknown function |
| YGR017W |
YGR017W |
Pyridoxamine 5'-phosphate oxidase; Uncharacterized protein YGR017W; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm |
| YGR018C |
YGR018C |
Uncharacterized protein YGR018C; Protein of unknown function; mRNA identified as translated by ribosome profiling data; partiy overlaps the uncharacterized ORF YGR017W |
| UGA1 |
YGR019W |
4-aminobutyrate aminotransferase; Gamma-aminobutyrate (GABA) transaminase; also known as 4-aminobutyrate aminotransferase; involved in the 4-aminobutyrate and glutamate degradation pathways; required for normal oxidative stress tolerance and nitrogen utilization; protein abundance increases in response to DNA replication stress; Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family |
| VMA7 |
YGR020C |
Subunit F of the V1 peripheral membrane domain of V-ATPase; part of the electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane; the V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase) has eight subunits |
| DPC29 |
YGR021W |
Probable transcriptional regulatory protein HAH1; Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies |
| MTL1 |
YGR023W |
Protein MTL1; Putative plasma membrane sensor; involved in cell integrity signaling and stress response during glucose starvation and oxidative stress; has structural and functional similarity to Mid2p; MTL1 has a paralog, MID2, that arose from the whole genome duplication |
| THG1 |
YGR024C |
tRNAHis guanylyltransferase; adds a guanosine residue to the 5' end of tRNAH is after transcription and RNase P cleavage; can also catalyze reverse (3'-5') polymerization with certain substrates in a template-dependent reaction; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes |
| YGR025W |
YGR025W |
Uncharacterized protein YGR025W; Putative protein of unknown function; conserved across S. cerevisiae strains |
| YGR026W |
YGR026W |
Uncharacterized membrane protein YGR026W; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery |
| RPS25A |
YGR027C |
Protein component of the sm (40S) ribosomal subunit; homologous to mammalian ribosomal protein S25, no bacterial homolog; RPS25A has a paralog, RPS25B, that arose from the whole genome duplication |
| YNCG0022W |
YNCG0022W |
Unknown |
| MSP1 |
YGR028W |
Protein MSP1; Highly-conserved N-terminy anchored AAA-ATPase; distributed in the mitochondrial outer membrane and peroxisomes; involved in mitochondrial protein sorting; functions as an extraction engine in local organelle surveillance to remove and initiate degradation of mistargeted proteins, ensuring fidelity of organelle-specific localization of tail-anchored proteins; contains an N-terminal transmembrane domain and C-terminal cytoplasmic ATPase domain |
| ERV1 |
YGR029W |
Flavin-linked sulfhydryl oxidase of the mitochondrial IMS; N-terminus is an intrinsicy disordered domain that in the cytosol helps target Erv1p to mitochondria, and in the intermembrane space oxidizes Mia40p as part of a disulfide relay system that promotes intermembrane space retention of imported proteins; functional ortholog of human GFER (ALR); human GFER carrying N-terminal 21 amino acids of Erv1p functiony complements the lethality of the erv1 null mutation |
| SNR46 |
YNCG0024W |
Unknown |
| POP6 |
YGR030C |
Ribonucleases P/MRP protein subunit POP6; Subunit of RNase MRP, nuclear RNase P and telomerase; forms a soluble heterodimer with Pop7p that binds P3 domain of RNase MRP and RNase P RNAs; RNase MRP cleaves pre-rRNA, nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs, while telomerase replenishes telomeric DNA; relocalizes to the cytosol in response to hypoxia |
| IMO32 |
YGR031W |
Abhydrolase domain-containing protein IMO32; Conserved mitochondrial protein of unknown function; processed by both mitochondrial processing peptidase and mitochondrial octapeptidyl aminopeptidase; gene contains the nested antisense gene NAG1; Belongs to the AB hydrolase superfamily |
| NAG1 |
YGR031C-A |
Protein involved in yeast cell w biogenesis; localizes to the cell periphery; production of Nag1p is dependent upon the presence of Slt2p and Rlm1p; gene is nested within and antisense to IMO32 |
| GSC2 |
YGR032W |
Catalytic subunit of 1,3-beta-glucan synthase; involved in formation of the inner layer of the spore w; activity positively regulated by Rho1p and negatively by Smk1p; GSC2 has a paralog, FKS1, that arose from the whole genome duplication; Belongs to the glycosyltransferase 48 family |
| TIM21 |
YGR033C |
Mitochondrial import inner membrane translocase subunit TIM21; Nonessential component of the TIM23 complex; interacts with the Translocase of the Outer Mitochondrial membrane (TOM complex) and with respiratory enzymes; may regulate the Translocase of the Inner Mitochondrial membrane (TIM23 complex) activity |
| RPL26B |
YGR034W |
Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; non-essential even when paralog is also deleted; deletion has minimal affections on ribosome biosynthesis; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication |
| YGR035C |
YGR035C |
Uncharacterized protein YGR035C; Putative protein of unknown function, potential Cdc28p substrate; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; YGR035C has a paralog, YLR346C, that arose from the whole genome duplication |
| YGR035W-A |
YGR035W-A |
Uncharacterized protein YGR035W-A; Putative protein of unknown function |
| CAX4 |
YGR036C |
Dolichyldiphosphatase; Dolichyl pyrophosphate (Dol-P-P) phosphatase; has a luminy oriented active site in the ER; cleaves the anhydride linkage in Dol-P-P; required for Dol-P-P-linked oligosaccharide intermediate synthesis and protein N-glycosylation |
| ACB1 |
YGR037C |
Diazepam-binding inhibitor (gaba receptor modulator, acyl-coa-binding protein); Acyl-CoA-binding protein; transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress |
| ORM1 |
YGR038W |
Protein that mediates sphingolipid homeostasis; evolutionarily conserved, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; ORM1 has a paralog, ORM2, that arose from the whole genome duplication |
| YNCG0025C |
YNCG0025C |
Unknown |
| YGR039W |
YGR039W |
Uncharacterized protein YGR039W; Putative protein of unknown function; conserved among S. cerevisiae strains; YGR039W is not an essential gene |
| KSS1 |
YGR040W |
Mitogen-activated protein kinase (MAPK); involved in signal transduction pathways that control filamentous growth and pheromone response; regulates septum assembly, and may directly phosphorylate Bni4p; the KSS1 gene is nonfunctional in S288C strains and functional in W303 strains |
| BUD9 |
YGR041W |
Protein involved in bud-site selection; mutant has increased aneuploidy tolerance; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the distal pole; BUD9 has a paralog, BUD8, that arose from the whole genome duplication; To yeast BUD8 |
| MTE1 |
YGR042W |
Uncharacterized protein YGR042W; Protein of unknown function; involved in maintenance of proper telomere length; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; forms nuclear foci upon DNA replication stress |
| NQM1 |
YGR043C |
Transaldolase of unknown function; transcription is repressed by Mot1p and induced by alpha-factor and during diauxic shift; NQM1 has a paralog, TAL1, that arose from the whole genome duplication |
| RME1 |
YGR044C |
Zinc finger protein involved in control of meiosis; prevents meiosis by repressing IME1 expression and promotes mitosis by activating CLN2 expression; directly repressed by a1-alpha2 regulator; mediates cell type control of sporulation; relocalizes from nucleus to cytoplasm upon DNA replication stress |
| YGR045C |
YGR045C |
Uncharacterized protein YGR045C; Putative protein of unknown function; conserved across S. cerevisiae strains |
| TAM41 |
YGR046W |
Mitochondrial phosphatidate cytidylyltransferase (CDP-DAG synthase); required for cardiolipin biosynthesis; viability of null mutant is strain-dependent; mRNA is targeted to the bud; mutant displays defect in mitochondrial protein import, likely due to altered membrane lipid composition; Belongs to the TAM41 family |
| TFC4 |
YGR047C |
Subunit of RNA polymerase III transcription initiation factor complex; one of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA domain of TFIIIC that binds BoxA DNA promoter sites of tRNA and similar genes; has TPR motifs; human homolog is TFIIIC-102 |
| UFD1 |
YGR048W |
Ubiquitin fusion degradation protein 1; Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transcription factors by regulated Ub/proteasome-dependent processing (RUP) |
| SCM4 |
YGR049W |
Protein SCM4; Mitochondrial outer membrane protein of unknown function; predicted to have 4 transmembrane segments; import is mediated by Tom70p and Mim1p; interacts geneticy with a cdc4 mutation; SCM4 has a paralog, ATG33, that arose from the whole genome duplication |
| YGR050C |
YGR050C |
Uncharacterized protein YGR050C; Protein of unknown function; mRNA identified as translated by ribosome profiling data |
| FMP48 |
YGR052W |
Probable serine/threonine-protein kinase FMP48; Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation |
| MCO32 |
YGR053C |
Uncharacterized protein YGR053C; Putative protein of unknown function |
| YGR054W |
YGR054W |
Eukaryotic initiation factor eIF2A; associates specificy with both 40S subunits and 80 S ribosomes, and interacts geneticy with both eIF5b and eIF4E; homologous to mammalian eIF2A |
| MUP1 |
YGR055W |
High affinity methionine permease; integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake |
| RSC1 |
YGR056W |
Chromatin structure-remodeling complex subunit RSC1; Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook; RSC1 has a paralog, RSC2, that arose from the whole genome duplication; Belongs to the RSC1 family |
| LST7 |
YGR057C |
Protein LST7; Subunit of the Lst4p-Lst7p GTPase activating protein complex for Gtr2p; stimulates the GTPase activity of Rag family GTPase Gtr2p, within the context of the Gtr1p-Gtr2p heterodimer, after amino acid stimulation; required for activation of TORC1 in response to amino acid stimulation; recruited to the vacuolar membrane during amino acid starvation and released from the membrane by TORC1; required for the transport of amino acid permease Gap1p from the Golgi to the cell surface |
| PEF1 |
YGR058W |
Peflin; Penta-EF-hand protein; required for polar bud growth and cell w abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly |
| SPR3 |
YGR059W |
Sporulation-regulated protein 3; Sporulation-specific homolog of the CDC3/10/11/12 family of genes; septin protein involved in sporulation; regulated by ABFI; the yeast CDC3/10/11/12 family is a family of bud neck microfilament genes; Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin- like GTPase superfamily. Septin GTPase family |
| SNR48 |
YNCG0026W |
Unknown |
| ERG25 |
YGR060W |
Methylsterol monooxygenase; C-4 methyl sterol oxidase; catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol; human MSMO1 functiony complements the growth defect caused by repression of ERG25 expression |
| ADE6 |
YGR061C |
Phosphoribosylformylglycinamidine synthase; Formylglycinamidine-ribonucleotide (FGAM)-synthetase; catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway |
| COX18 |
YGR062C |
Protein required for membrane insertion of C-terminus of Cox2p; mitochondrial integral inner membrane protein; interacts geneticy and physicy with Mss2p and Pnt1p; similar to S. cerevisiae Oxa1, N. crassa Oxa2p, and E. coli YidC; respiratory defect of the null mutant is functiony complemented by human COX18 carrying the N-terminal 54 amino acids of S. cerevisiae Cox18p; Belongs to the OXA1/ALB3/YidC family |
| SPT4 |
YGR063C |
Spt4p/5p (DSIF) transcription elongation factor complex subunit; the Spt4/5 complex binds to ssRNA in a sequence-specific manner, and along with RNAP I and II has multiple roles regulating transcriptional elongation, RNA processing, quality control, and transcription-coupled repair; localizes to kinetochores and heterochromatin, influencing chromosomal dynamics and silencing; required for transcription through long trinucleotide repeats in ORFs and non-protein coding regions |
| VHT1 |
YGR065C |
Vitamin H transporter; High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin |
| GID10 |
YGR066C |
Glucose-induced degradation protein 4; Uncharacterized protein YGR066C; Putative protein of unknown function |
| YGR067C |
YGR067C |
Zinc finger protein ygr067c; Putative protein of unknown function; contains a zinc finger motif similar to that of Adr1p |
| ART5 |
YGR068C |
Arrestin-related trafficking adapter 5; Protein proposed to regulate endocytosis of plasma membrane proteins; regulates by recruiting the ubiquitin ligase Rsp5p to its target in the plasma membrane; SWAT-GFP and mCherry fusion proteins localize to the cytosol |
| ROM1 |
YGR070W |
Guanine nucleotide exchange factor (GEF) for Rho1p; mutations are syntheticy lethal with mutations in rom2, which also encodes a GEP; ROM1 has a paralog, ROM2, that arose from the whole genome duplication |
| ENV11 |
YGR071C |
Late endosome and vacuole interface protein 11; Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and fragmented vacuoles; deletion mutant has increased glycogen accumulation and displays elongated buds; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; ENV11 has a paralog, VID22, that arose from the whole genome duplication |
| UPF3 |
YGR072W |
Component of the nonsense-mediated mRNA decay (NMD) pathway; along with Nam7p and Nmd2p; involved in decay of mRNA containing nonsense codons; involved in telomere maintenance |
| SMD1 |
YGR074W |
Sm nuclear ribonucleoprotein Sm D1; Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; relocalizes to the cytosol in response to hypoxia; homolog of human Sm D1; protein abundance increases in response to DNA replication stress |
| PRP38 |
YGR075C |
Pre-mRNA-splicing factor 38; Unique component of the U4/U6.U5 tri-snRNP particle; tri-snRNP is required for conformational changes which result in the catalytic activation of the spliceosome; dispensable for spliceosome assembly; Belongs to the PRP38 family |
| MRPL25 |
YGR076C |
Mitochondrial 54s ribosomal protein yml25; Mitochondrial ribosomal protein of the large subunit; mutation confers increased replicative lifespan |
| PEX8 |
YGR077C |
Peroxisomal biogenesis factor 8; Intraperoxisomal organizer of the peroxisomal import machinery; organizes the formation of the importomer complex, bridging the docking complex with the RING finger complex; tightly associated with the lumenal face of the peroxisomal membrane; essential for peroxisome biogenesis; binds PTS1-signal receptor Pex5p, and PTS2-signal receptor Pex7p |
| PAC10 |
YGR078C |
Prefoldin subunit 3; Part of the heteromeric co-chaperone GimC/prefoldin complex; complex promotes efficient protein folding; Belongs to the prefoldin subunit alpha family |
| YGR079W |
YGR079W |
Putative uncharacterized protein ygr079w; Putative protein of unknown function; YGR079W is not an essential gene |
| TWF1 |
YGR080W |
Twinfilin-1; Twinfilin; highly conserved actin monomer-sequestering protein involved in regulation of the cortical actin cytoskeleton; coordinates actin filament severing and monomer sequestering at sites of rapid actin turnover; composed of two cofilin-like regions, stimulates actin depolymerization as does the mouse homolog, mTwf1 |
| SLX9 |
YGR081C |
Ribosome biogenesis protein SLX9; Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S sm ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant |
| TOM20 |
YGR082W |
Mitochondrial import receptor subunit TOM20; Component of the TOM (translocase of outer membrane) complex; responsible for recognition and initial import steps for mitochondriy directed proteins; acts as a receptor for incoming precursor proteins |
| GCD2 |
YGR083C |
Delta subunit of the translation initiation factor eIF2B; the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; Belongs to the eIF-2B alpha/beta/delta subunits family |
| MRP13 |
YGR084C |
37S ribosomal protein MRP13, mitochondrial; Mitochondrial ribosomal protein of the sm subunit |
| RPL11B |
YGR085C |
Ribosomal 60S subunit protein L11B; expressed at half the level of Rpl11Ap; involved in ribosomal assembly; depletion causes degradation of 60S proteins and RNA; homologous to mammalian ribosomal protein L11 and bacterial L5; RPL11B has a paralog, RPL11A, that arose from the whole genome duplication |
| PIL1 |
YGR086C |
Sphingolipid long chain base-responsive protein PIL1; Eisosome core component; eisosomes are large immobile cell cortex structures associated with endocytosis; detected in phosphorylated state in mitochondria; phosphorylated on Thr233 upon Pkc1p hyperactivation in a Slt2p MAPK-dependent fashion; null mutant shows activation of Pkc1p/Ypk1p stress resistance pathways; member of BAR domain family; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress |
| PDC6 |
YGR087C |
Minor isoform of pyruvate decarboxylase; decarboxylates pyruvate to acetaldehyde, involved in amino acid catabolism; transcription is glucose- and ethanol-dependent, and is strongly induced during sulfur limitation; Belongs to the TPP enzyme family |
| CTT1 |
YGR088W |
Cytosolic catalase T; has a role in protection from oxidative damage by hydrogen peroxide |
| NNF2 |
YGR089W |
Protein that exhibits physical and genetic interactions with Rpb8p; Rpb8p is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation |
| YNCG0027W |
YNCG0027W |
Unknown |
| UTP22 |
YGR090W |
U3 sm nucleolar RNA-associated protein 22; Component of the sm-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals |
| PRP31 |
YGR091W |
Pre-mRNA-processing factor 31; Splicing factor; component of the U4/U6-U5 snRNP complex; Belongs to the PRP31 family |
| DBF2 |
YGR092W |
Cell cycle protein kinase DBF2; Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis; DBF2 has a paralog, DBF20, that arose from the whole genome duplication |
| DRN1 |
YGR093W |
CWF19-like protein DRN1; Splicing factor that modulates turnover of branched RNAs by Dbr1p; interacts with spliceosomal components and branched RNA splicing products; enhances Dbr1p debranching in vitro; conserved protein with domain organization identical from yeast to human; N-terminal homology to Dbr1p N-terminus, but Dbr1p catalytic residues not conserved; relocalizes to the cytosol in response to hypoxia |
| VAS1 |
YGR094W |
Valine--tRNA ligase, mitochondrial; Mitochondrial and cytoplasmic valyl-tRNA synthetase; human homolog VARS2 implicated in mitochondrial diseases, can partiy complement yeast null mutant |
| RRP46 |
YGR095C |
Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) |
| TPC1 |
YGR096W |
Mitochondrial thiamine pyrophosphate carrier 1; Mitochondrial membrane transporter; mediates uptake of the essential cofactor thiamine pyrophosphate (ThPP) into mitochondria; expression appears to be regulated by carbon source; member of the mitochondrial carrier family |
| ASK10 |
YGR097W |
Activator of SKN7 protein 10; Regulator of the Fps1p glycerol channel; under nonstress conditions, binds to Fps1p to positively regulate glycerol transport; under osmotic stress, multiple phosphorylation by Hog1p causes Ask10p to dissociate from Fps1p; forms homodimers and heterodimerizes with paralog Rgc1p; phosphorylated in response to oxidative stress; has a role in destruction of Ssn8p; associates with RNA polymerase II holoenzyme |
| ESP1 |
YGR098C |
Separin; Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress |
| TEL2 |
YGR099W |
Telomere length regulation protein TEL2; Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1; Belongs to the TEL2 family |
| MDR1 |
YGR100W |
Cytoplasmic GTPase-activating protein; activates Ypt/Rab transport GTPases Ypt6p, Ypt31p and Sec4p; involved in recycling of internalized proteins and regulation of Golgi secretory function |
| PCP1 |
YGR101W |
Rhomboid protein 1, mitochondrial; Mitochondrial serine protease; required for the processing of various mitochondrial proteins and maintenance of mitochondrial DNA and morphology; belongs to the rhomboid-GlpG superfamily of intramembrane peptidases |
| GTF1 |
YGR102C |
Glutamyl-tRNA(Gln) amidotransferase subunit F, mitochondrial; Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; transposon insertion mutant is salt sensitive and null mutant has growth defects; non-tagged protein is detected in purified mitochondria |
| NOP7 |
YGR103W |
Mrna-binding ribosome synthesis protein nop7; Pescadillo homolog; Component of several different pre-ribosomal particles; forms a complex with Ytm1p and Erb1p that is required for maturation of the large ribosomal subunit; required for exit from G<sub>0</sub> and the initiation of cell proliferation |
| SRB5 |
YGR104C |
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance |
| VMA21 |
YGR105W |
Integral membrane protein required for V-ATPase function; not an actual component of the vacuolar H+-ATPase (V-ATPase) complex; diverged ortholog of human XMEA (X-linked Myopathy with Excessive Autophagy); functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER) |
| VOA1 |
YGR106C |
ER protein that functions in assembly of the V0 sector of V-ATPase; functions with other assembly factors; null mutation enhances the vacuolar ATPase (V-ATPase) deficiency of a vma21 mutant impaired in endoplasmic reticulum (ER) retrieval; Belongs to the VOA1 family |
| YNCG0028W |
YNCG0028W |
Unknown |
| YNCG0029C |
YNCG0029C |
Unknown |
| CLB1 |
YGR108W |
G2/mitotic-specific cyclin-1; B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome; CLB1 has a paralog, CLB2, that arose from the whole genome duplication |
| CLB6 |
YGR109C |
S-phase entry cyclin-6; B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1; CLB6 has a paralog, CLB5, that arose from the whole genome duplication |
| YNCG0030C |
YNCG0030C |
Unknown |
| CLD1 |
YGR110W |
Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58; Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily |
| YGR111W |
YGR111W |
Uncharacterized protein YGR111W; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus |
| SHY1 |
YGR112W |
Cytochrome oxidase assembly protein SHY1; Mitochondrial inner membrane protein required for complex IV assembly; associates with complex IV assembly intermediates and complex III/complex IV supercomplexes; similar to human SURF1 involved in Leigh Syndrome; complex IV is also known as cytochrome c oxidase |
| DAM1 |
YGR113W |
Essential subunit of the Dam1 complex (aka DASH complex); cooperates with Duo1p to connect the DASH complex with the microtubules (MT); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments |
| SPT6 |
YGR116W |
Chromatin-remodeling histone chaperone spt6; Transcription elongation factor SPT6; Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region; required for H3K36 trimethylation but not dimethylation by Set2p |
| YGR117C |
YGR117C |
Uncharacterized protein YGR117C; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm |
| RPS23A |
YGR118W |
Ribosomal protein 28 (rp28) of the sm (40S) ribosomal subunit; required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23A has a paralog, RPS23B, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal |
| NUP57 |
YGR119C |
FG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of the nuclear pore complex (NPC) permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) |
| COG2 |
YGR120C |
Essential component of the conserved oligomeric Golgi complex; a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments; the components of the Golgi complex are Gog1p through Cog8p |
| YNCG0031W |
YNCG0031W |
Unknown |
| MEP1 |
YGR121C |
Ammonium transporter MEP1; Ammonium permease; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation; human homolog RHCG complements yeast null mutant; mutations in human homolog RHCG implicated in metabolic acidosis; MEP1 has a paralog, MEP3, that arose from the whole genome duplication |
| YGR121W-A |
YGR121W-A |
Uncharacterized protein YGR121W-A; Putative protein of unknown function |
| YGR122W |
YGR122W |
Uncharacterized protein YGR122W; Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress |
| YNCG0032W |
YNCG0032W |
Unknown |
| PPT1 |
YGR123C |
Protein serine/threonine phosphatase; regulates Hsp90 chaperone by affecting its ATPase and cochaperone binding activities; has similarity to human phosphatase PP5; present in both the nucleus and cytoplasm; expressed during logarithmic growth |
| YNCG0033W |
YNCG0033W |
Unknown |
| ASN2 |
YGR124W |
Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN2 has a paralog, ASN1, that arose from the whole genome duplication |
| VSB1 |
YGR125W |
Uncharacterized vacuolar membrane protein YGR125W; Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole |
| YGR126W |
YGR126W |
Uncharacterized protein YGR126W; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS |
| YGR127W |
YGR127W |
Uncharacterized protein YGR127W; Putative protein of unknown function; expression is regulated by Msn2p/Msn4p, indicating a possible role in stress response |
| UTP8 |
YGR128C |
U3 sm nucleolar RNA-associated protein 8; Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the sm subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA |
| SYF2 |
YGR129W |
Pre-mRNA-splicing factor SYF2; Member of the NineTeen Complex (NTC); NTC contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; relocalizes to the cytosol in response to hypoxia; isy1 syf2 cells have defective spindles activiating cell cycle arrest |
| YGR130C |
YGR130C |
Uncharacterized protein YGR130C; Component of the eisosome with unknown function; GFP-fusion protein localizes to the cytoplasm; specificy phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7) |
| FHN1 |
YGR131W |
Non-classical export protein 2 homolog 1; Protein of unknown function; induced by ketoconazole; promoter region contains sterol regulatory element motif, which has been identified as a Upc2p-binding site; overexpression complements function of Nce102p in NCE102 deletion strain; FHN1 has a paralog, NCE102, that arose from the whole genome duplication |
| PHB1 |
YGR132C |
Prohibitin-1; Subunit of the prohibitin complex (Phb1p-Phb2p); prohibitin is a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation; prohibitin deficiency induces a mitochondrial unfolded protein response (mtUPR) |
| PEX4 |
YGR133W |
Ubiquitin-conjugating enzyme E2-21 kDa; Peroxisomal ubiquitin conjugating enzyme; required for peroxisomal matrix protein import and peroxisome biogenesis |
| CAF130 |
YGR134W |
Protein CAF130; Subunit of the CCR4-NOT transcriptional regulatory complex; CCR4-NOT complex is evolutionarily-conserved and involved in controlling mRNA initiation, elongation, and degradation |
| PRE9 |
YGR135W |
Alpha 3 subunit of the 20S proteasome; the only nonessential 20S subunit; may be replaced by the alpha 4 subunit (Pre6p) under stress conditions to create a more active proteasomal isoform; Belongs to the peptidase T1A family |
| LSB1 |
YGR136W |
LAS seventeen-binding protein 1; Negative regulator of actin nucleation-promoting factor activity; interacts with Las17p, a homolog of human Wiskott-Aldrich Syndrome protein (WASP), via an N-terminal SH3 domain, and along with PIN3 cooperatively inhibits the nucleation of actin filaments; overexpression blocks receptor-mediated endocytosis; protein increases in abundance and forms nuclear foci in response to DNA replication stress; LSB1 has a paralog, PIN3, that arose from the whole genome duplication |
| TPO2 |
YGR138C |
Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; transcription of TPO2 is regulated by Haa1p; TPO2 has a paralog, TPO3, that arose from the whole genome duplication |
| CBF2 |
YGR140W |
Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; sumoylated in an Mms21p-dependent manner; relative distribution to the spindle pole body decreases upon DNA replication stress |
| VPS62 |
YGR141W |
Vacuolar protein sorting (VPS) protein; required for cytoplasm to vacuole targeting of proteins; VPS62 has a paralog, TDA6, that arose from the whole genome duplication |
| BTN2 |
YGR142W |
v-SNARE binding protein; facilitates specific protein retrieval from a late endosome to the Golgi; modulates arginine uptake, possible role in mediating pH homeostasis between the vacuole and plasma membrane H(+)-ATPase; contributes to prion curing; preferentiy expressed after severe ethanol stress |
| YNCG0034W |
YNCG0034W |
Unknown |
| SKN1 |
YGR143W |
Protein involved in sphingolipid biosynthesis; type II membrane protein; SKN1 has a paralog, KRE6, that arose from the whole genome duplication |
| SUF4 |
YNCG0035W |
Unknown |
| THI4 |
YGR144W |
Thiazole synthase; abundant protein involved in the formation of the thiazole moiety of thiamine during thiamine biosynthesis; acts more as a co-substrate rather than an enzyme by providing the sulphur source for thiazole formation; undergoes a single turnover only; required for mitochondrial genome stability in response to DNA damaging agents |
| ENP2 |
YGR145W |
Ribosome biogenesis protein ENP2; Component of the SSU; required for pre-18S rRNA processing, biogenesis of the sm ribosomal subunit; interacts with U3 snoRNA, Mpp10p and Bfr2p; contains WD repeats, and has homology to Spb1p |
| ECL1 |
YGR146C |
Protein of unknown function; mitochondrial-dependent role in the extension of chronological lifespan; overexpression increases oxygen consumption and respiratory activity while deletion results in reduced oxygen consumption under conditions of caloric restriction; induced by iron homeostasis transcription factor Aft2p; multicopy suppressor of temperature sensitive hsf1 mutant; induced by treatment with 8-methoxypsoralen and UVA irradiation |
| YGR146C-A |
YGR146C-A |
Uncharacterized protein YGR146C-A; Putative protein of unknown function |
| NAT2 |
YGR147C |
Putative N-terminal acetyltransferase 2; Protein of unknown function; has an apparent role in acetylation of N-terminal methionine residues |
| RPL24B |
YGR148C |
Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication |
| GPC1 |
YGR149W |
Uncharacterized membrane protein YGR149W; Glycerophosphocholine acyltransferase (GPCAT); involved in in phosphatidylcholine (PC) synthesis; uses acetyl-CoA to acylate glycero-3-phosphocholine to yield lyso-PC; also catalyzes acylation of glycerophosphoethanolamine with acyl-CoA; predicted to be an integal membrane protein |
| CCM1 |
YGR150C |
Mitochondrial group I intron splicing factor CCM1; Mitochondrial 15S rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; has separable roles in stabilizing mitochondrial 15S rRNA and in maturation of the COB and COX1 mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport |
| YNCG0036W |
YNCG0036W |
Unknown |
| RSR1 |
YGR152C |
Ras-related protein RSR1; GTP-binding protein of the Ras superfamily; required for bud site selection, morphological changes in response to mating pheromone, and efficient cell fusion; localized to the plasma membrane; significantly similar to mammalian Rap GTPases |
| YGR153W |
YGR153W |
Uncharacterized protein YGR153W; Putative protein of unknown function |
| GTO1 |
YGR154C |
Glutathione S-transferase omega-like 1; Omega-class glutathione transferase; induced under oxidative stress; putative peroxisomal localization |
| CYS4 |
YGR155W |
Cystathionine beta-synthase; catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog CBS cause homocystinuria; human CBS can complement yeast null mutant |
| PTI1 |
YGR156W |
Protein PTI1; Essential component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; relocalizes to the cytosol in response to hypoxia; similar to mammalian Cleavage-Stimulation Factor CstF-64 |
| CHO2 |
YGR157W |
Phosphatidylethanolamine n-methyltransferase; Phosphatidylethanolamine methyltransferase (PEMT); catalyzes the first step in the conversion of phosphatidylethanolamine to phosphatidylcholine during the methylation pathway of phosphatidylcholine biosynthesis |
| MTR3 |
YGR158C |
Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) |
| NSR1 |
YGR159C |
Nucleolar protein that binds nuclear localization sequences; required for pre-rRNA processing and ribosome biogenesis; binds to single stranded telomeric DNA and mRNA; methylated by Hmt1p; interaction with Top1p and nucleolar localization are negatively regulated by polyphosphorylation |
| RTS3 |
YGR161C |
Protein phosphatase type 2a regulatory subunit rts3; Putative component of the protein phosphatase type 2A complex |
| YGR161W-C |
YGR161W-C |
Uncharacterized protein YGR161W-C; Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species |
| YNCG0037W |
YNCG0037W |
Unknown |
| TIF4631 |
YGR162W |
Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication |
| GTR2 |
YGR163W |
GTP-binding protein GTR2; Subunit of a TORC1-stimulating GTPase complex; subunit of the Gtr1-Gtr2 GTPase complex that stimulates TORC1 in response to amino acid stimulation; stimulates the GTPase activity of Gtr1p; negatively regulates the Ran/Tc4 GTPase cycle; activates transcription; tethered to the vacuolar membrane as part of the EGO complex (EGOC); required for sorting of Gap1p; activated by the the Lst4p-Lst7p GAP complex; localizes to cytoplasm and to chromatin; homolog of human RagC and |
| YGR164W |
YGR164W |
Uncharacterized protein YGR164W; Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data |
| YNCG0038C |
YNCG0038C |
Unknown |
| MRPS35 |
YGR165W |
Mitochondrial 37s ribosomal protein mrps35; Mitochondrial ribosomal protein of the sm subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets |
| TRS65 |
YGR166W |
Trafficking protein particle complex II-specific subunit 65; Component of transport protein particle (TRAPP) complex II; TRAPPII is a multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating intra-Golgi and endosome-Golgi traffic; role in cell w beta-glucan biosynthesis and the stress response |
| CLC1 |
YGR167W |
Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; regulates endocytic progression; thought to regulate clathrin function; the clathrin triskelion is a trimeric molecule composed of three heavy chains that radiate from a vertex and three light chains which bind noncovalently near the vertex of the triskelion |
| PEX35 |
YGR168C |
Uncharacterized protein ygr168c; Putative protein of unknown function; YGR168C is not an essential gene |
| PUS6 |
YGR169C |
tRNA:pseudouridine synthase; catalyzes the conversion of uridine to pseudouridine at position 31 in cytoplasmic and mitochondrial tRNAs; mutation of Asp168 to Ala abolishes enzyme activity; not essential for viability |
| LSO2 |
YGR169C-A |
Uncharacterized protein YGR169C-A; Protein with a potential role in response to iron deprivation; localizes to nucleus and cytoplasm, and nuclear localization is enhanced under iron-replete conditions; null mutant exhibits slow growth during iron deprivation; LSO2 has a paralog, LSO1, that arose from the whole genome duplication |
| PSD2 |
YGR170W |
Phosphatidylserine decarboxylase of the Golgi and vacuolar membranes; converts phosphatidylserine to phosphatidylethanolamine; controls vacuolar membrane phospholipid content by regulating phospholipids in compartments that will eventuy give rise to the vacuole; loss of Psd2p causes a specific reduction in vacuolar membrane PE levels while total PE levels are not significantly affected |
| MSM1 |
YGR171C |
Methionine--tRNA ligase, mitochondrial; Mitochondrial methionyl-tRNA synthetase (MetRS); functions as a monomer in mitochondrial protein synthesis; functions similarly to cytoplasmic MetRS although the cytoplasmic form contains a zinc-binding domain not found in Msm1p |
| YIP1 |
YGR172C |
Integral membrane protein; required for the biogenesis of ER-derived COPII transport vesicles; interacts with Yif1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; human homolog YIPF5 can complement yeast yip1 mutant |
| RBG2 |
YGR173W |
Ribosome-interacting GTPase 2; Protein with a role in translation; forms a complex with Gir2p; has similarity to mammalian developmenty regulated GTP-binding protein; Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family |
| CBP4 |
YGR174C |
Assembly factor CBP4; Mitochondrial protein required for assembly of cytochrome bc1 complex; interacts with the Cbp3p-Cbp6p complex and newly synthesized cytochrome b (Cobp) to promote assembly of Cobp into the cytochrome bc1 complex |
| ERG1 |
YGR175C |
Squalene epoxidase; catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine; human SQLE functiony complements the lethality of the erg1 null mutation |
| ATF2 |
YGR177C |
Alcohol O-acetyltransferase 2; Alcohol acetyltransferase; may play a role in steroid detoxification; forms volatile esters during fermentation, which is important for brewing and winemaking |
| PBP1 |
YGR178C |
PAB1-binding protein 1; Component of glucose deprivation induced stress granules; involved in P-body-dependent granule assembly; similar to human ataxin-2; interacts with Pab1p to regulate mRNA polyadenylation; interacts with Mkt1p to regulate HO translation; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress |
| OKP1 |
YGR179C |
Central kinetochore subunit OKP1; Outer kinetochore protein required for accurate chromosome segregation; component of COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) a kinetochore sub-complex which functions as a platform for kinetochore assembly; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-Q and fission yeast fta7 |
| RNR4 |
YGR180C |
Ribonucleoside-diphosphate reductase sm chain 2; Ribonucleotide-diphosphate reductase (RNR) sm subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the sm subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress; RNR4 has a paralog, RNR2, that arose from the whole genome duplication |
| YNCG0040C |
YNCG0040C |
Unknown |
| TIM13 |
YGR181W |
Mitochondrial import inner membrane translocase subunit TIM13; Mitochondrial intermembrane space protein; forms a complex with Tim8p that delivers a subset of hydrophobic proteins to the TIM22 complex for insertion into the inner membrane |
| QCR9 |
YGR183C |
Subunit 9 of ubiquinol cytochrome-c reductase (Complex III); Complex III is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex; Belongs to the UQCR10/QCR9 family |
| UBR1 |
YGR184C |
E3 ubiquitin ligase (N-recognin); heterodimerizes with Rad6p to recognize and ubiquitinate substrates of the N-end rule pathway; role in endoplasmic reticulum-associated protein degradation (ERAD) in the absence of canonical ER membrane ligases or after stress; major role in targeting misfolded cytosolic proteins for degradation; regulates peptide transport via Cup9p ubiquitination; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS) |
| TYS1 |
YGR185C |
Tyrosine--tRNA ligase, cytoplasmic; Cytoplasmic tyrosyl-tRNA synthetase; required for cytoplasmic protein synthesis; interacts with positions 34 and 35 of the tRNATyr anticodon; mutations in human ortholog YARS are associated with Charcot-Marie-Tooth (CMT) neuropathies; human ortholog YARS functiony complements the heat sensitivity of a ts ele; protein abundance increases in response to DNA replication stress; Belongs to the class-I aminoacyl-tRNA synthetase family |
| TFG1 |
YGR186W |
TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74 |
| HGH1 |
YGR187C |
Nonessential protein of unknown function; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; similar to mammalian BRP16 (Brain protein 16); relative distribution to the nucleus increases upon DNA replication stress |
| BUB1 |
YGR188C |
Protein kinase involved in the cell cycle checkpoint into anaphase; in complex with Mad1p and Bub3p, prevents progression into anaphase in presence of spindle damage; Cdc28p-mediated phosphorylation at Bub1p-T566 is important for degradation in anaphase and adaptation of checkpoint to prolonged mitotic arrest; associates with centromere DNA via Skp1p; involved in Sgo1p relocalization in response to sister kinetochore tension; paralog MAD3 arose from whole genome duplication |
| HIP1 |
YGR191W |
Yeast amino acid transporter; High-affinity histidine permease; also involved in the transport of manganese ions |
| TDH3 |
YGR192C |
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), isozyme 3; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell w; GAPDH-derived antimicrobial peptides secreted by S. cerevisiae are active against a wide variety of wine-related yeasts and bacteria; binds AU-rich RNA |
| PDX1 |
YGR193C |
E3-binding protein of the mitochondrial pyruvate dehydrogenase complex; plays a structural role in the complex by binding and positioning dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide acetyltransferase (E2) core |
| XKS1 |
YGR194C |
Xylulokinase; converts D-xylulose and ATP to xylulose 5-phosphate and ADP; rate limiting step in fermentation of xylulose; required for xylose fermentation by recombinant S. cerevisiae strains |
| SKI6 |
YGR195W |
Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4) |
| FYV8 |
YGR196C |
Protein fyv8; Protein of unknown function; required for survival upon exposure to K1 killer toxin |
| SNG1 |
YGR197C |
Protein involved in resistance to nitrosoguanidine and 6-azauracil; expression is regulated by transcription factors involved in multidrug resistance; SNG1 has a paralog, YJR015W, that arose from the whole genome duplication |
| YPP1 |
YGR198W |
Cargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4p; is required, along with Efr3p, for the assembly and recruitment of multiple copies of the kinase into phosphoinositide kinase (PIK) patches at the plasma membrane; positively regulates Stt4p; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene |
| PMT6 |
YGR199W |
Dolichyl-phosphate-mannose--protein mannosyltransferase 6; Protein O-mannosyltransferase; transfers mannose from dolichyl phosphate-D-mannose to protein serine/threonine residues of secretory proteins; reaction is essential for cell w rigidity; member of a family of mannosyltransferases; Belongs to the glycosyltransferase 39 family |
| ELP2 |
YGR200C |
Subunit of Elongator complex; binds to microtubules via conserved alkaline residues; has two seven-bladed WD40 β propellers; Elongator complex is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin |
| YGR201C |
YGR201C |
Putative elongation factor 1 gamma homolog; Putative protein of unknown function |
| PCT1 |
YGR202C |
Cholinephosphate cytidylyltransferase; a rate-determining enzyme of the CDP-choline pathway for phosphatidylcholine synthesis, inhibited by Sec14p, activated upon lipid-binding; contains an element within the regulatory domain involved in both silencing and activation of enzymatic activity |
| YCH1 |
YGR203W |
CDC25-like phosphatase YCH1; Phosphatase with sequence similarity to Cdc25p; Arr2p and Mih1p; member of the single-domain rhodanese homology superfamily; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus |
| ADE3 |
YGR204W |
Trifunctional formate-tetrahydrofolate ligase/methenyltetrahydrofolate cyclohydrolase/methylenetetrahydrofolate dehydrogenase ade3; C-1-tetrahydrofolate synthase, cytoplasmic; Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase; involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine |
| YGR204C-A |
YGR204C-A |
Uncharacterized protein YGR204C-A; Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching |
| TDA10 |
YGR205W |
Probable ATP-dependent kinase TDA10; ATP-binding protein of unknown function; crystal structure resembles that of E.coli pantothenate kinase and other sm kinases; null mutant is sensitive to expression of the top1-T722A ele |
| MVB12 |
YGR206W |
Multivesicular body sorting factor 12; ESCRT-I subunit required to stabilize ESCRT-I core complex oligomers; the ESCRT-I core complex (Stp22p, Vps28p, Srn2p) is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin |
| CIR1 |
YGR207C |
Probable electron transfer flavoprotein subunit beta; Mitochondrial protein that interacts with frataxin (Yfh1p); putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-beta; may have a role in oxidative stress response |
| SER2 |
YGR208W |
Phosphoserine phosphatase of the phosphoglycerate pathway; involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source; Belongs to the HAD-like hydrolase superfamily. SerB family |
| TRX2 |
YGR209C |
Thioredoxin-2; Cytoplasmic thioredoxin isoenzyme; part of thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p; required for ER-Golgi transport and vacuole inheritance; with Trx1p, facilitates mitochondrial import of sm Tims Tim9p, Tim10p, Tim13p by maintaining them in reduced form; abundance increases under DNA replication stress; TRX2 has a paralog, TRX1, that arose from the whole genome duplication |
| YGR210C |
YGR210C |
Uncharacterized GTP-binding protein YGR210C; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm |
| ZPR1 |
YGR211W |
Essential protein with two zinc fingers; present in nucleus of growing cells, relocates to cytoplasm in starved cells via a process mediated by Cpr1p; binds translation elongation factor eEF-1 (Tef1p); relative distribution to nucleus increases upon DNA replication stress; human ZPR1 gene can complement yeast by owing growth during down-regulation of yeast zpr1 |
| SLI1 |
YGR212W |
N-acetyltransferase sli1; N-acetyltransferase; confers resistance to the sphingolipid biosynthesis inhibitor myriocin (ISP-1) by converting it into N-acetyl-myriocin, co-operates with Ypk1p in mediating resistance to myriocin |
| RTA1 |
YGR213C |
Protein involved in 7-aminocholesterol resistance; has seven potential membrane-spanning regions; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of protein; RTA1 has a paralog, YLR046C, that arose from the whole genome duplication |
| RPS0A |
YGR214W |
Ribosomal 40S subunit protein S0A; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2; RPS0A has a paralog, RPS0B, that arose from the whole genome duplication; |
| RSM27 |
YGR215W |
Mitochondrial 37s ribosomal protein rsm27; Mitochondrial ribosomal protein of the sm subunit |
| GPI1 |
YGR216C |
Phosphatidylinositol N-acetylglucosaminyltransferase subunit GPI1; Membrane protein involved in the synthesis of GlcNAc-PI; N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI) is the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; human and mouse GPI1p are functional homologs |
| CCH1 |
YGR217W |
Calcium-channel protein CCH1; Voltage-gated high-affinity calcium channel; involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and partiy co-localizes with Mid1p; however, evidence suggests CCH1 is not required for Mid1p function |
| CRM1 |
YGR218W |
Exportin-1; Major karyopherin; involved in export of proteins, RNAs, and ribosomal subunits from the nucleus; exportin |
| MRPL9 |
YGR220C |
Mitochondrial 54s ribosomal protein yml9; Mitochondrial ribosomal protein of the large subunit |
| TOS2 |
YGR221C |
Protein involved in localization of Cdc24p to the site of bud growth; may act as a membrane anchor; localizes to the bud neck and bud tip; potentiy phosphorylated by Cdc28p; TOS2 has a paralog, SKG6, that arose from the whole genome duplication; Belongs to the SKG6/TOS2 family |
| SNR7-L |
YNCG0044C |
Unknown |
| SNR7-S |
YNCG0045C |
Unknown |
| PET54 |
YGR222W |
Protein PET54; Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing |
| HSV2 |
YGR223C |
SVP1-like protein 2; Phosphatidylinositol 3,5-bisphosphate-binding protein; plays a role in micronucleophagy; belongs to the PROPPIN family of proteins; predicted to fold as a seven-bladed beta-propeller; displays punctate cytoplasmic localization; Belongs to the WD repeat SVP1 family |
| AZR1 |
YGR224W |
Azole resistance protein 1; Plasma membrane transporter of the major facilitator superfamily; involved in resistance to azole drugs such as ketoconazole and fluconazole |
| AMA1 |
YGR225W |
Meiosis-specific APC/C activator protein AMA1; Activator of meiotic anaphase promoting complex (APC/C); Cdc20p family member; required for initiation of spore w assembly; required for Clb1p degradation during meiosis; prevents premature assembly of the meiosis I spindle, required for DSB induced prophase I arrest; Belongs to the WD repeat CDC20/Fizzy family |
| DIE2 |
YGR227W |
Dolichyl-phosphoglucose-dependent alpha-1,2-glucosyltransferase; located in the ER; functions in pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation; has a role in regulation of ITR1 and INO1; human homolog ALG10B can complement yeast die2 null mutant |
| OTO1 |
YGR227C-A |
Unknown |
| SMI1 |
YGR229C |
Cell w assembly regulator SMI1; Protein involved in the regulation of cell w synthesis; proposed to be involved in coordinating cell cycle progression with cell w integrity |
| BNS1 |
YGR230W |
Protein of unknown function; overexpression bypasses need for Spo12p, but not required for meiosis; BNS1 has a paralog, SPO12, that arose from the whole genome duplication |
| PHB2 |
YGR231C |
Prohibitin-2; Subunit of the prohibitin complex (Phb1p-Phb2p); prohibitin is a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation; prohibitin deficiency induces a mitochondrial unfolded protein response (mtUPR) |
| NAS6 |
YGR232W |
Probable 26S proteasome regulatory subunit p28; Evolutionarily conserved 19S regulatory particle assembly-chaperone; proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); ortholog of human oncoprotein gankyrin, also known as p28, which interacts with the Rb tumor suppressor and CDK4/6 |
| PHO81 |
YGR233C |
Phosphate system positive regulatory protein PHO81; Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress |
| YHB1 |
YGR234W |
Flavohemoprotein; Nitric oxide oxidoreductase; flavohemoglobin that plays role in oxidative and nitrosative stress responses; protects against nitration of cellular targets and against cell growth inhibition under aerobic or anaerobic conditions; yeast flavohemoglobin Yhb1p and human homolog neuroglobin NGB protect cells against alpha-synuclein cytotoxicity and aggregate formation; protein increases in abundance, relocalizes from nucleus to cytoplasmic foci upon DNA replication stress; In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family |
| MIC26 |
YGR235C |
MICOS subunit MIC26; Component of the MICOS complex; MICOS (formerly MINOS or MitOS) is a mitochondrial inner membrane complex that extends into the intermembrane space and has a role in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane; Mic26p is a non-essential component of the complex |
| SPG1 |
YGR236C |
Stationary phase gene 1 protein; Protein required for high temperature survival during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies |
| YGR237C |
YGR237C |
Uncharacterized protein YGR237C; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm |
| KEL2 |
YGR238C |
Kelch repeat-containing protein 2; Protein that negatively regulates mitotic exit; forms a complex with Kel1p and Bud14p that regulates Bnr1p (formin) to affect actin cable assembly, cytokinesis, and polarized growth; functions in a complex with Kel1p, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate |
| PEX21 |
YGR239C |
Peroxin required for peroxisomal matrix protein targeting; acts on proteins containing the PTS2 targeting sequence; interacts with Pex7p; constitutively expressed; partiy redundant with Pex18p; required for import of the Gpd1p-Pnc1p heterodimer in which only Gpd1p has a peroxisomal targeting signal; relative distribution to cytoplasmic foci increases upon DNA replication stress; Belongs to the peroxin-21 family |
| PFK1 |
YGR240C |
Alpha subunit of heterooctameric phosphofructokinase; involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes; Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily |
| YGR240C-A |
YGR240C-A |
Uncharacterized protein YGR240C-A; Putative protein of unknown function; identified by fungal homology and RT-PCR |
| YAP1802 |
YGR241C |
Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1802 has a paralog, YAP1801, that arose from the whole genome duplication; Belongs to the AP180 family |
| MPC3 |
YGR243W |
Highly conserved subunit of the mitochondrial pyruvate carrier (MPC); expressed during growth on nonfermentable carbon sources, and heterodimerizes with Mpc1p to form the respiratory isoform of MPC; MPC localizes to the mitochondrial inner membrane and mediates pyruvate uptake; MPC3 paralog, MPC2, heterodimerizes with Mpc1p to form the fermentative MPC isoform; protein abundance increases in response to DNA replication stress |
| LSC2 |
YGR244C |
Succinate--coa ligase [adp-forming] subunit beta, mitochondrial; Beta subunit of succinyl-CoA ligase; succinyl-CoA ligase is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate |
| SDA1 |
YGR245C |
Protein required for actin organization and passage through Start; highly conserved nuclear protein; required for actin cytoskeleton organization; plays a critical role in G1 events; binds Nap1p; involved in 60S ribosome biogenesis; Belongs to the SDA1 family |
| BRF1 |
YGR246C |
TFIIIB B-related factor; one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB; mutations in human homolog are associated with autosomal recessive cerebellar-facial-dental syndrome; Belongs to the TFIIB family |
| CPD1 |
YGR247W |
2',3'-cyclic-nucleotide 3'-phosphodiesterase; Cyclic nucleotide phosphodiesterase; hydrolyzes ADP-ribose 1'', 2''-cyclic phosphate to ADP-ribose 1''-phosphate; may have a role in tRNA splicing; no detectable phenotype is conferred by null mutation or by overexpression; protein abundance increases in response to DNA replication stress |
| SOL4 |
YGR248W |
6-phosphogluconolactonase; protein abundance increases in response to DNA replication stress; SOL4 has a paralog, SOL3, that arose from the whole genome duplication |
| MGA1 |
YGR249W |
Protein mga1; Protein similar to heat shock transcription factor; multicopy suppressor of pseudohyphal growth defects of ammonium permease mutants |
| RIE1 |
YGR250C |
Uncharacterized RNA-binding protein YGR250C; Putative RNA binding protein; localizes to stress granules induced by glucose deprivation; interacts with Rbg1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress |
| NOP19 |
YGR251W |
Nucleolar protein 19; Ribosome biogenesis factor; nucleolar protein associated with pre-rRNA components of the 90S preribosome, required for cleavage of pre-rRNA at A0, A1 and A2 sites; interacts with RNA helicase Dhr2p and RNA helicase-like protein Utp25p; required for incorporation of Utp25p into preribosomes |
| GCN5 |
YGR252W |
Catalytic subunit of ADA and SAGA histone acetyltransferase complexes; modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; relocalizes to the cytosol in response to hypoxia; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation; Belongs to the acetyltransferase family. GCN5 subfamily |
| PUP2 |
YGR253C |
Alpha 5 subunit of the 20S proteasome; involved in ubiquitin-dependent catabolism; human homolog is subunit zeta; Belongs to the peptidase T1A family |
| ENO1 |
YGR254W |
Enolase I, a phosphopyruvate hydratase; catalyzes conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression repressed in response to glucose; protein abundance increases in response to DNA replication stress; N-terminy propionylated in vivo; ENO1 has a paralog, ENO2, that arose from the whole genome duplication |
| COQ6 |
YGR255C |
Flavin-dependent monooxygenase involved in ubiquinone biosynthesis; responsible for hydroxylation at position C5 and deamination at C4 during ubiquinone (Coenzyme Q) biosynthesis; localizes to matrix face of mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes; human homolog COQ6 can complement yeast null mutant and is implicated in steroid-resistant nephrotic syndrome (SRNS); Belongs to the UbiH/COQ6 family |
| YNCG0046W |
YNCG0046W |
Unknown |
| GND2 |
YGR256W |
6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone; GND2 has a paralog, GND1, that arose from the whole genome duplication |
| MTM1 |
YGR257C |
Mitochondrial protein of the mitochondrial carrier family; high affinity pyridoxal 5'-phosphate (PLP) transporter, important for delivery of PLP cofactor to mitochondrial enzymes; involved in mitochondrial iron homeostasis and in activating mitochondrial Sod2p by facilitating insertion of an essential manganese cofactor |
| RAD2 |
YGR258C |
DNA repair protein RAD2; Single-stranded DNA endonuclease; cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein |
| TNA1 |
YGR260W |
High affinity nicotinic acid plasma membrane permease; responsible for uptake of low levels of nicotinic acid; expression of the gene increases in the absence of extracellular nicotinic acid or para-aminobenzoate (PABA) |
| APL6 |
YGR261C |
Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and periphery associated membrane-bound pools |
| BUD32 |
YGR262C |
Protein kinase; component of the EKC/KEOPS complex with Kae1p, Cgi121p, Pcc1p, and Gon7p; Pyrococcus Bud32 ortholog functions as a P-loop ATPase rather than a protein kinase in the context of the complex; EKC/KEOPS complex is required for t6A tRNA modification and telomeric TG1-3 recombination; may have role in transcription; mutation is functiony complemented by human TP53RK |
| SAY1 |
YGR263C |
Steryl acetyl hydrolase 1; Sterol deacetylase; component of the sterol acetylation/deacetylation cycle along with Atf2p; active both in the endoplasmic reticulum (ER) and in lipid droplets; integral membrane protein with active site in the ER lumen; green fluorescent protein (GFP)-fusion protein localizes to the ER |
| MES1 |
YGR264C |
Methionine--tRNA ligase, cytoplasmic; Methionyl-tRNA synthetase; forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs; mutations in human ortholog MARS are associated with pediatric pulmonary alveolar proteinosis |
| YGR266W |
YGR266W |
Uncharacterized protein YGR266W; Protein of unknown function; predicted to contain a single transmembrane domain; mutant has increased aneuploidy tolerance; localized to both the mitochondrial outer membrane and the plasma membrane; protein abundance increases in response to DNA replication stress |
| FOL2 |
YGR267C |
GTP-cyclohydrolase I, catalyzes first step in folic acid biosynthesis; human homolog GCH1 is implicated in dopa-responsive dystonia (DRD), and can complement yeast null mutant |
| HUA1 |
YGR268C |
Proline-rich protein HUA1; Cytoplasmic protein containing a zinc finger domain; sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly |
| YTA7 |
YGR270W |
Tat-binding homolog 7; Protein that localizes to chromatin; has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; relocalizes to the cytosol in response to hypoxia; potentiy phosphorylated by Cdc28p |
| SLH1 |
YGR271W |
Antiviral helicase SLH1; Putative RNA helicase related to Ski2p; involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses; Belongs to the helicase family. SKI2 subfamily |
| EFG1 |
YGR271C-A |
rRNA-processing protein EFG1; Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus |
| YGR273C |
YGR273C |
Uncharacterized protein YGR273C; Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; not an essential gene; YGR273C has a paralog, YMR295C, that arose from the whole genome duplication; To yeast YMR295c |
| TAF1 |
YGR274C |
TFIID subunit, involved in RNA pol II transcription initiation; possesses in vitro histone acetyltransferase activity but its role in vivo appears to be minor; involved in promoter binding and G1/S progression; relocalizes to the cytosol in response to hypoxia |
| RTT102 |
YGR275W |
Regulator of Ty1 transposition protein 102; Component of both the SWI/SNF and RSC chromatin remodeling complexes; suggested role in chromosome maintenance; possible weak regulator of Ty1 transposition; protein abundance increases in response to DNA replication stress |
| RNH70 |
YGR276C |
RNA exonuclease 1; 3'-5' exoribonuclease; required for maturation of 3' ends of 5S rRNA and tRNA-Arg3 from dicistronic transcripts |
| CAB4 |
YGR277C |
Phosphopantetheine adenylyltransferase; Subunit of the CoA-Synthesizing Protein Complex (CoA-SPC); subunits of this complex are: Cab2p, Cab3p, Cab4p, Cab5p, Sis2p and Vhs3p; probable pantetheine-phosphate adenylyltransferase (PPAT); PPAT catalyzes the fourth step in the biosynthesis of coenzyme A from pantothenate; null mutant lethality is complemented by E. coli coaD (encoding PPAT) and by human COASY |
| CWC22 |
YGR278W |
U2-type spliceosomal complex subunit CWC22; Pre-mRNA-splicing factor CWC22; Spliceosome-associated protein that is required for pre-mRNA splicing; necessary for Prp2p function at the first catalytic step of splicing; has similarity to S. pombe Cwf22p; CWC22 is an essential protein |
| SCW4 |
YGR279C |
Probable family 17 glucosidase SCW4; Cell w protein with similarity to glucanases; scw4 scw10 double mutants exhibit defects in mating; SCW4 has a paralog, SCW10, that arose from the whole genome duplication; Belongs to the glycosyl hydrolase 17 family |
| PXR1 |
YGR280C |
Essential protein involved in rRNA and snoRNA maturation; competes with TLC1 RNA for binding to Est2p, suggesting a role in negative regulation of telomerase; human homolog inhibits telomerase; contains a G-patch RNA interacting domain |
| YOR1 |
YGR281W |
Oligomycin resistance ATP-dependent permease YOR1; Plasma membrane ATP-binding cassette (ABC) transporter; multidrug transporter mediates export of many different organic anions including oligomycin; homolog of human cystic fibrosis transmembrane receptor (CFTR); Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily |
| BGL2 |
YGR282C |
Endo-beta-1,3-glucanase; major protein of the cell w, involved in cell w maintenance; involved in incorporation of newly synthesized mannoprotein molecules into the cell w |
| YGR283C |
YGR283C |
Putative methyltransferase; may interact with ribosomes, based on co-purification experiments; predicted to be involved in ribosome biogenesis; null mutant is resistant to fluconazole; GFP-fusion protein localizes to the nucleolus; YGR283C has a paralog, YMR310C, that arose from the whole genome duplication; Belongs to the class IV-like SAM-binding methyltransferase superfamily |
| ERV29 |
YGR284C |
Protein localized to COPII-coated vesicles; involved in vesicle formation and incorporation of specific secretory cargo; protein abundance increases in response to DNA replication stress; Belongs to the SURF4 family |
| ZUO1 |
YGR285C |
Zuotin; Ribosome-associated chaperone; zuotin functions in ribosome biogenesis and as a chaperone for nascent polypeptide chains in partnership with Ssz1p and SSb1/2; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p; human gene DNAJC2 can partiy complement yeast zuo1 null mutant |
| BIO2 |
YGR286C |
Biotin synthase, mitochondrial; Biotin synthase; catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant; Belongs to the radical SAM superfamily. Biotin synthase family |
| IMA1 |
YGR287C |
Oligo-1,6-glucosidase IMA1; Major isomaltase (alpha-1,6-glucosidase/alpha-methylglucosidase); required for isomaltose utilization; preferred specificity for isomaltose, alpha-methylglucoside, and palatinose, but also exhibits alpha-1,2 glucosidase activity on sucrose and kojibiose, and can cleave the 1,3-alpha linkage of nigerose and turanose and the alpha-1,5 linkage of leucrose in vitro; member of the IMA isomaltase family; Belongs to the glycosyl hydrolase 13 family |
| MAL13 |
YGR288W |
Maltose fermentation regulatory protein MAL13; MAL-activator protein; part of complex locus MAL1; nonfunctional in genomic reference strain S288C |
| MAL32 |
YBR299W |
Alpha-glucosidase MAL32; Maltase (alpha-D-glucosidase); inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose; Belongs to the glycosyl hydrolase 13 family |
| PAU24 |
YBR301W |
Seripauperin-24; Cell w mannoprotein; has similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth; Belongs to the SRP1/TIP1 family. Seripauperin subfamily |
| COS8 |
YHL048W |
Endosomal protein involved in turnover of plasma membrane proteins; member of the DUP380 subfamily of conserved, often subtelomeric COS genes; required for the multivesicular vesicle body sorting pathway that internalizes plasma membrane proteins for degradation; Cos proteins provide ubiquitin in trans for nonubiquitinated cargo proteins; Belongs to the DUP/COS family |
| ARN2 |
YHL047C |
Mfs transporter, sit family, siderophore-iron:h+ symporter; Siderophore iron transporter ARN2; Transporter; member of the ARN family of transporters that specificy recognize siderophore-iron chelates; responsible for uptake of iron bound to the siderophore triacetylfusarinine C |
| PAU13 |
YHL046C |
Seripauperin-13; Protein of unknown function; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expression is induced after ethanol shock; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum and vacuole respectively; Belongs to the SRP1/TIP1 family. Seripauperin subfamily |
| YHL044W |
YHL044W |
DUP240 protein YHL044W; Putative integral membrane protein; member of DUP240 gene family; green fluorescent protein (GFP)-fusion protein localizes to the plasma membrane in a punctate pattern |
| ECM34 |
YHL043W |
Protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomericy-encoded proteins; SWAT-GFP, seamless-GFP and mCherry C-terminal fusion proteins localize to the cytosol |
| YHL042W |
YHL042W |
Putative uncharacterized protein YHL042W; Protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomericy-encoded proteins; SWAT-GFP and mCherry fusion proteins localize to the vacuole |
| YHL041W |
YHL041W |
Uncharacterized protein YHL041W; Putative protein of unknown function; conserved across S. cerevisiae strains |
| ARN1 |
YHL040C |
Mfs transporter, sit family, siderophore-iron:h+ symporter; ARN family transporter for siderophore-iron chelates; responsible for uptake of iron bound to ferrirubin, ferrirhodin, and related siderophores; protein increases in abundance and relocalizes to the vacuole upon DNA replication stress |
| EFM1 |
YHL039W |
Protein-lysine N-methyltransferase EFM1; Lysine methyltransferase; involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm |
| CBP2 |
YHL038C |
Cytochrome B pre-mRNA-processing protein 2; Required for splicing of the group I intron bI5 of the COB pre-mRNA; nuclear-encoded mitochondrial protein that binds to the RNA to promote splicing; also involved in but not essential for splicing of the COB bI2 intron and the intron in the 21S rRNA gene |
| YHL037C |
YHL037C |
Uncharacterized protein YHL037C; Putative protein of unknown function; conserved among S. cerevisiae strains |
| MUP3 |
YHL036W |
Low-affinity methionine permease; Low affinity methionine permease; similar to Mup1p |
| VMR1 |
YHL035C |
ABC transporter ATP-binding protein/permease VMR1; Vacuolar membrane protein; involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions; VMR1 has a paralog, YBT1, that arose from the whole genome duplication |
| SBP1 |
YHL034C |
Single-stranded nucleic acid-binding protein; Protein that binds eIF4G and has a role in repression of translation; has an RGG motif; found in cytoplasmic P bodies; binds to mRNAs under glucose starvation stress, most often in the 5' UTR; found associated with sm nucleolar RNAs snR10 and snR11; SBP1 has a paralog, RNP1, that arose from the whole genome duplication; Belongs to the RRM GAR family |
| RPL8A |
YHL033C |
Ribosomal 60S subunit protein L8A; required for processing of 27SA3 pre-rRNA to 27SB pre-rRNA during assembly of large ribosomal subunit; depletion leads to a turnover of pre-rRNA; L8 binds to Domain I of 25S and 5.8 S rRNAs; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication |
| GUT1 |
YHL032C |
Glycerol kinase; converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p; Belongs to the FGGY kinase family |
| GOS1 |
YHL031C |
Golgi SNAP receptor complex member 1; v-SNARE protein involved in Golgi transport; homolog of the mammalian protein GOS-28/GS28 |
| ECM29 |
YHL030W |
Proteasome component ECM29; Scaffold protein; assists in association of the proteasome core particle with the regulatory particle; inhibits proteasomal ATPase activity; degraded by the mature proteasome after assembly; contains HEAT-like repeats; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress; Belongs to the ECM29 family |
| OCA5 |
YHL029C |
Oxidant-induced cell-cycle arrest protein 5; Cytoplasmic protein required for replication of Brome mosaic virus; S. cerevisiae is a model system for studying replication of positive-strand RNA viruses in their natural hosts; Belongs to the OCA5 family |
| WSC4 |
YHL028W |
Cell w integrity and stress response component 4; Endoplasmic reticulum (ER) membrane protein; involved in the translocation of soluble secretory proteins and insertion of membrane proteins into the ER membrane; may also have a role in the stress response but has only partial functional overlap with WSC1-3 |
| RIM101 |
YHL027W |
pH-response transcription factor pacC/RIM101; Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to alkaline conditions; involved in cell w assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC; Belongs to the pacC/RIM101 family |
| YHL026C |
YHL026C |
Uncharacterized protein YHL026C; Putative protein of unknown function; transcriptiony regulated by Upc2p via an upstream sterol response element; SWAT-GFP fusion protein localizes to the cell periphery, while mCherry fusion localizes to both the cell periphery and vacuole; YHL026C is not an essential gene; in 2005 the start site was moved 141 nt upstream (see Locus History) |
| SNF6 |
YHL025W |
Transcription regulatory protein SNF6; Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions |
| RIM4 |
YHL024W |
Meiotic activator RIM4; Putative RNA-binding protein; required for the expression of early and middle sporulation genes |
| NPR3 |
YHL023C |
Nitrogen permease regulator 3; Subunit of the Iml1p/SEACIT complex; SEACIT (Iml1p-Npr2p-Npr3p) is a subcomplex of SEAC, a coatomer-related complex that associates dynamicy with the vacuole; Npr3p may have a structural or regulatory role, supporting Iml1p function as a GAP for the Rag family GTPase Gtr1p, and leading to inhibition of TORC1 signaling in response to amino acid deprivation; SEACIT is required for non-nitrogen-starvation-induced autophagy; null mutant has meiotic defects; human NPRL3 homolog |
| YNCH0001W |
YNCH0001W |
Unknown |
| SPO11 |
YHL022C |
Meiosis-specific protein that initiates meiotic recombination; initiates meiotic recombination by catalyzing the formation of double-strand breaks in DNA via a transesterification reaction; required for homologous chromosome pairing and synaptonemal complex formation |
| AIM17 |
YHL021C |
Probable oxidoreductase AIM17; Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss; Belongs to the gamma-BBH/TMLD family |
| OPI1 |
YHL020C |
Transcriptional repressor OPI1; Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance; null exhibits disrupted mitochondrial metabolism and low cardiolipin content, strongly correlated with overproduction of inositol; binds to phosphatidic acid |
| APM2 |
YHL019C |
Protein of unknown function; homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport; Belongs to the adaptor complexes medium subunit family |
| MCO14 |
YHL018W |
Putative pterin-4-alpha-carbinolamine dehydratase; Putative 4a-hydroxytetrahydrobiopterin dehydratase; green fluorescent protein (GFP)-fusion protein localizes to mitochondria and is induced in response to the DNA-damaging agent MMS |
| YHL017W |
YHL017W |
Uncharacterized membrane protein YHL071W; Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein co-localizes with clathrin-coated vesicles; YHL017W has a paralog, PTM1, that arose from the whole genome duplication; Belongs to the LU7TM family |
| DUR3 |
YHL016C |
Plasma membrane transporter for both urea and polyamines; expression is highly sensitive to nitrogen catabolite repression and induced by ophanate, the last intermediate of the antoin degradative pathway; Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family |
| RPS20 |
YHL015W |
Protein component of the sm (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 |
| YLF2 |
YHL014C |
Obg-like ATPase homolog; Protein of unknown function; has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies |
| OTU2 |
YHL013C |
OTU domain-containing protein 2; Protein of unknown function; may interact with ribosomes, based on co-purification experiments; member of the ovarian tumor-like (OTU) superfamily of predicted cysteine proteases; shows cytoplasmic localization; protein abundance increases in response to DNA replication stress |
| YHL012W |
YHL012W |
Probable UTP--glucose-1-phosphate uridylyltransferase; Putative UTP glucose-1-phosphate uridylyltransferase; YHL012W has a paralog, UGP1, that arose from the whole genome duplication |
| PRS3 |
YHL011C |
Ribose-phosphate pyrophosphokinase 3; 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase; synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; Belongs to the ribose-phosphate pyrophosphokinase family |
| ETP1 |
YHL010C |
RING finger protein ETP1; Protein of unknown function required for growth on ethanol; contains a zinc finger region and has homology to human BRAP2, which is a cytoplasmic protein that binds nuclear localization sequences |
| YAP3 |
YHL009C |
AP-1-like transcription factor YAP3; Basic leucine zipper (bZIP) transcription factor |
| YNCH0002C |
YNCH0002C |
Unknown |
| YHL008C |
YHL008C |
Uncharacterized transporter YHL008C; Putative protein of unknown function; may be involved in the uptake of chloride ions; does not appear to be involved in monocarboxylic acid transport; green fluorescent protein (GFP)-fusion protein localizes to the vacuole |
| STE20 |
YHL007C |
Serine/threonine-protein kinase STE20; Cdc42p-activated signal transducing kinase; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p; member of the PAK (p21-activated kinase) family |
| SHU1 |
YHL006C |
Suppressor of HU sensitivity involved in recombination protein 1; Component of Shu complex (aka PCSS complex); Shu complex also includes Psy3, Csm2, Shu2, and promotes error-free DNA repair, mediates inhibition of Srs2p function; essential for promoting the establishment of homolog bias during meiotic homologous recombination; promotes both crossover (CO) and non-crossover (NCO) pathways of meiotic recombination and formation of Rad51p filaments |
| YHL005C |
YHL005C |
Uncharacterized protein YHL005C; Putative protein of unknown function; conserved among S. cerevisiae strains; YHL005C is not an essential gene |
| MRP4 |
YHL004W |
37S ribosomal protein MRP4, mitochondrial; Mitochondrial ribosomal protein of the sm subunit |
| LAG1 |
YHL003C |
Sphingosine N-acyltransferase LAG1; Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functiony equivalent to Lac1p; forms ER foci upon DNA replication stress; homolog of human CERS2, a tumor metastasis suppressor gene whose silencing enhances invasion/metastasis of prostate cancer cells; LAG1 has a paralog, LAC1, that arose from the whole genome duplication |
| HSE1 |
YHL002W |
Class E vacuolar protein-sorting machinery protein HSE1; Subunit of the endosomal Vps27p-Hse1p complex; complex is required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes |
| RPL14B |
YHL001W |
Ribosomal 60S subunit protein L14B; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14B has a paralog, RPL14A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress |
| OSH7 |
YHR001W |
Oxysterol-binding protein; part of family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability; OSH7 has a paralog, OSH6, that arose from the whole genome duplication |
| QCR10 |
YHR001W-A |
Subunit of the ubiqunol-cytochrome c oxidoreductase complex; this complex comprises part of the mitochondrial respiratory chain; members include Cobp, Rip1p, Cyt1p, Cor1p, Qcr2p, Qcr6p, Qcr7p, Qcr8p, Qcr9p, and Qcr10p and comprises part of the mitochondrial respiratory chain; Belongs to the UQCR11/QCR10 family |
| LEU5 |
YHR002W |
Mitochondrial carrier protein; involved in the accumulation of CoA in the mitochondrial matrix; homolog of human Graves disease protein SLC25A16, which complements yeast null mutant; does not encode an isozyme of Leu4p, as first hypothesized |
| TCD1 |
YHR003C |
tRNA threonylcarbamoyladenosine dehydratase; required for the ct6A tRNA base modification, where an adenosine at position 37 is modified to form a cyclized active ester with an oxazolone ring; localized to the mitochondrial outer membrane; TCD1 has a paralog, TCD2, that arose from the whole genome duplication |
| NEM1 |
YHR004C |
Nuclear envelope morphology protein 1; Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard; Belongs to the Dullard family |
| GPA1 |
YHR005C |
Guanine nucleotide-binding protein alpha-1 subunit; Subunit of the G protein involved in pheromone response; GTP-binding alpha subunit of the heterotrimeric G protein; negatively regulates the mating pathway by sequestering G(beta)gamma and by triggering an adaptive response; activates Vps34p at the endosome; protein abundance increases in response to DNA replication stress |
| TIM10 |
YHR005C-A |
Mitochondrial import inner membrane translocase subunit TIM10; Essential protein of the mitochondrial intermembrane space; forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane |
| YNCH0003C |
YNCH0003C |
Unknown |
| STP2 |
YHR006W |
Transcription factor; activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes; STP2 has a paralog, STP1, that arose from the whole genome duplication |
| ERG11 |
YHR007C |
Lanosterol 14-alpha-demethylase; catalyzes C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in ergosterol biosynthesis pathway; transcriptiony down-regulated when ergosterol is in excess; member of cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p; human CYP51A1 functiony complements the lethality of the erg11 null mutation |
| YHR007C-A |
YHR007C-A |
Uncharacterized protein YHR007C-A; Putative protein of unknown function; identified by expression profiling and mass spectrometry; SWAT-GFP fusion protein localizes to the nucleus |
| SOD2 |
YHR008C |
Mitochondrial manganese superoxide dismutase; protects cells against oxygen toxicity and oxidative stress; human mitochondrial SOD2 can complement a yeast null mutant and human cytoplasmic SOD1 can also complement when targeted to the mitochondrial matrix |
| TDA3 |
YHR009C |
Putative oxidoreductase involved in late endosome to Golgi transport; physical and genetical interactions with Btn2p; null mutant is viable, has extended S phase, and sensitive to expression of top1-T722A ele; similar to human FOXRED1 |
| RPL27A |
YHR010W |
Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication |
| DIA4 |
YHR011W |
Serine--tRNA ligase, mitochondrial; Probable mitochondrial seryl-tRNA synthetase; mutant displays increased invasive and pseudohyphal growth; Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily |
| VPS29 |
YHR012W |
Vacuolar protein sorting-associated protein 29; Subunit of the membrane-associated retromer complex; endosomal protein; essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval; Belongs to the VPS29 family |
| ARD1 |
YHR013C |
Subunit of protein N-terminal acetyltransferase NatA; NatA comprises Nat1p, Ard1p, Nat5p; acetylates many proteins to influence telomeric silencing, cell cycle, heat-shock resistance, mating, sporulation, early stages of mitophagy; protein abundance increases under DNA replication stress; mutations in human homolog X-linked NAA10 lead to Ogden syndrome (S37P) and intellectual disability (R116W); expression of human NAA10 and NAA15 can complement ard1 nat1 double mutant |
| SPO13 |
YHR014W |
Meiosis-specific protein SPO13; Meiotic regulator; involved in maintaining sister chromatid cohesion during meiosis I as well as promoting proper attachment of kinetochores to the spindle during meiosis I and meiosis II; anaphase-promoting complex (APC) substrate that is degraded during anaphase I; expressed only in meiotic cells |
| YNCH0004C |
YNCH0004C |
Unknown |
| YNCH0005W |
YNCH0005W |
Unknown |
| MIP6 |
YHR015W |
Putative RNA-binding protein; interacts with Mex67p, which is a component of the nuclear pore involved in nuclear mRNA export; MIP6 has a paralog, PES4, that arose from the whole genome duplication |
| YSC84 |
YHR016C |
Actin-binding protein; involved in bundling of actin filaments and endocytosis of actin cortical patches; activity stimulated by Las17p; contains SH3 domain similar to Rvs167p; YSC84 has a paralog, LSB3, that arose from the whole genome duplication |
| YSC83 |
YHR017W |
UPF0744 protein YSC83; Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83 |
| ARG4 |
YHR018C |
Argininosuccinate lyase; catalyzes the final step in the arginine biosynthesis pathway; Belongs to the lyase 1 family. Argininosuccinate lyase subfamily |
| DED81 |
YHR019C |
Asparagine--tRNA ligase, cytoplasmic; Cytosolic asparaginyl-tRNA synthetase; required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA; Belongs to the class-II aminoacyl-tRNA synthetase family |
| YHR020W |
YHR020W |
Putative proline--tRNA ligase YHR020W; Prolyl-tRNA synthetase; N-terminal domain shows weak homology to prokaryotic posttransfer editing domain, but does not possess posttransfer editing activity; may interact with ribosomes, based on co-purification experiments |
| YNCH0006C |
YNCH0006C |
Unknown |
| RPS27B |
YHR021C |
Protein component of the sm (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27B has a paralog, RPS27A, that arose from the whole genome duplication |
| ECM12 |
YHR021W-A |
Protein ecm12; Putative protein of unknown function; may contribute to cell w biosynthesis, mutants display zymolyase hypersensitivity |
| YHR022C |
YHR022C |
Uncharacterized protein yhr022c; Putative protein of unknown function; YHR022C is not an essential gene |
| YHR022C-A |
YHR022C-A |
Uncharacterized protein YHR022C-A; Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching |
| MYO1 |
YHR023W |
Myosin-1; Type II myosin heavy chain; required for wild-type cytokinesis and cell separation; localizes to the actomyosin ring; binds to myosin light chains Mlc1p and Mlc2p through its IQ1 and IQ2 motifs respectively |
| MAS2 |
YHR024C |
Alpha subunit of the mitochondrial processing protease (MPP); essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondriy imported proteins; Belongs to the peptidase M16 family |
| THR1 |
YHR025W |
Homoserine kinase; conserved protein required for threonine biosynthesis; long-lived protein that is preferentiy retained in mother cells and forms cytoplasmic filaments; expression is regulated by the GCN4-mediated general amino acid control pathway |
| VMA16 |
YHR026W |
Subunit c'' of the vacuolar ATPase; v-ATPase functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain |
| RPN1 |
YHR027C |
Non-ATPase base subunit of the 19S RP of the 26S proteasome; may participate in the recognition of several ligands of the proteasome; contains a leucine-rich repeat (LRR) domain, a site for protein-protein interactions; RP is the acronym for regulatory particle |
| DAP2 |
YHR028C |
Dipeptidyl aminopeptidase; synthesized as a glycosylated precursor; localizes to the vacuolar membrane; similar to Ste13p |
| YHI9 |
YHR029C |
Uncharacterized isomerase YHI9; Protein of unknown function; null mutant is defective in unfolded protein response; possibly involved in a membrane regulation metabolic pathway; member of the PhzF superfamily, though most likely not involved in phenazine production |
| SLT2 |
YHR030C |
Mitogen-activated protein kinase SLT2/MPK1; Serine/threonine MAP kinase; coordinates expression of 19S regulatory particle assembly-chaperones (RACs) to control proteasome abundance; involved in regulating maintenance of cell w integrity, cell cycle progression, nuclear mRNA retention in heat shock, septum assembly; required for mitophagy, pexophagy; affects recruitment of mitochondria to phagophore assembly site; plays role in adaptive response of cells to cold; regulated by the PKC1-mediated signaling pathway |
| RRM3 |
YHR031C |
DNA helicase involved in rDNA replication and Ty1 transposition; binds to and suppresses DNA damage at G4 motifs in vivo; relieves replication fork pauses at telomeric regions; structury and functiony related to Pif1p; Belongs to the helicase family |
| ERC1 |
YHR032W |
Ethionine resistance-conferring protein 1; Member of the multi-drug and toxin extrusion (MATE) family; the MATE family is part of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily; overproduction confers ethionine resistance and accumulation of S-adenosylmethionine |
| YHR033W |
YHR033W |
Uncharacterized protein YHR033W; Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YHR033W has a paralog, PRO1, that arose from the whole genome duplication |
| PIH1 |
YHR034C |
Protein interacting with Hsp90 1; Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II |
| NEL1 |
YHR035W |
Uncharacterized protein YHR035W; Activator of Sar1p GTPase activity; paralog of Sec23 but does not associate with the COPII components; not an essential gene |
| BRL1 |
YHR036W |
Nucleus export protein BRL1; Essential nuclear envelope/ER integral membrane protein; interacts and functions with Apq12p and Brr6p in lipid homeostasis; mutants are defective in nuclear pore complex biogenesis, mRNA nuclear export and are sensitive to sterol biosynthesis inhibitors and membrane fluidizing agents; identified as a dosage suppressor of a temperature sensitive mutation in the major karyopherin, CRM1; homologous to Brr6p; Belongs to the BRL1/BRR6 family |
| PUT2 |
YHR037W |
Delta-1-pyrroline-5-carboxylate dehydrogenase; nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of human homolog ALDH4A1 causes type II hyperprolinemia (HPII), an autosomal recessive inborn error of metabolism; human homolog ALDH4A1 can complement yeast null mutant |
| RRF1 |
YHR038W |
Ribosome-recycling factor, mitochondrial; Mitochondrial ribosome recycling factor; essential for mitochondrial protein synthesis and for the maintenance of the respiratory function of mitochondria |
| MSC7 |
YHR039C |
Putative aldehyde dehydrogenase-like protein YHR039C; Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids |
| VMA10 |
YHR039C-A |
Subunit G of the V1 peripheral membrane domain of V-ATPase; part of the electrogenic proton pump found throughout the endomembrane system; involved in vacuolar acidification; the V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase) has eight subunits |
| BCD1 |
YHR040W |
Box C/D snoRNA protein 1; Essential protein required for the accumulation of box C/D snoRNA |
| SRB2 |
YHR041C |
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance |
| NCP1 |
YHR042W |
NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p; In the N-terminal section; belongs to the flavodoxin family |
| DOG2 |
YHR043C |
2-deoxyglucose-6-phosphate phosphatase; member of a family of low molecular weight phosphatases, induced by oxidative and osmotic stress, confers 2-deoxyglucose resistance when overexpressed; DOG2 has a paralog, DOG1, that arose from a single-locus duplication; the last half of DOG1 and DOG2 are subject to gene conversions among S. cerevisiae, S. paradoxus, and S. mikatae |
| DOG1 |
YHR044C |
2-deoxyglucose-6-phosphate phosphatase; member of a family of low molecular weight phosphatases; confers 2-deoxyglucose resistance when overexpressed; DOG1 has a paralog, DOG2, that arose from a single-locus duplication; the last half of DOG1 and DOG2 are subject to gene conversions among S. cerevisiae, S. paradoxus, and S. mikatae |
| YHR045W |
YHR045W |
Putative protein of unknown function; possible role in iron metabolism and/or amino acid and carbohydrate metabolism; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum |
| INM1 |
YHR046C |
Inositol monophosphatase; involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate |
| AAP1 |
YHR047C |
Alanine/arginine aminopeptidase; Arginine/alanine amino peptidase; overproduction stimulates glycogen accumulation; AAP1 has a paralog, APE2, that arose from the whole genome duplication |
| YHK8 |
YHR048W |
Probable drug/proton antiporter YHK8; Presumed antiporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; expression of gene is up-regulated in cells exhibiting reduced susceptibility to azoles |
| FSH1 |
YHR049W |
Family of serine hydrolases 1; Putative serine hydrolase; localizes to both the nucleus and cytoplasm; sequence is similar to S. cerevisiae Fsh2p and Fsh3p and the human candidate tumor suppressor OVCA2 |
| SMF2 |
YHR050W |
Divalent metal ion transporter involved in manganese homeostasis; has broad specificity for di-valent and tri-valent metals; post-translationy regulated by levels of metal ions; member of the Nramp family of metal transport proteins |
| YHR050W-A |
YHR050W-A |
Putative uncharacterized protein YHR050W-A; Protein of unknown function; identified by expression profiling and mass spectrometry |
| COX6 |
YHR051W |
Subunit VI of cytochrome c oxidase (Complex IV); Complex IV is the terminal member of the mitochondrial inner membrane electron transport chain; expression is regulated by oxygen levels |
| CIC1 |
YHR052W |
Proteasome-interacting protein CIC1; Essential protein that interacts with proteasome components; has a potential role in proteasome substrate specificity; also copurifies with 66S pre-ribosomal particles |
| RUF5-1 |
YNCH0007W |
Unknown |
| YHR054C-B |
YHR054C-B |
Unknown |
| CUP1-2 |
YHR055C |
Metothionein; binds copper and mediates resistance to high concentrations of copper and cadmium; locus is variably amplified in different strains, with two copies, CUP1-1 and CUP1-2, in the genomic sequence reference strain S288C; CUP1-2 has a paralog, CUP1-1, that arose from a segmental duplication |
| RSC30 |
YHR056C |
Chromatin structure-remodeling complex protein RSC30; Component of the RSC chromatin remodeling complex; non-essential gene required for regulation of ribosomal protein genes and the cell w/stress response; null mutants are osmosensitive; RSC30 has a paralog, RSC3, that arose from the whole genome duplication |
| CPR2 |
YHR057C |
Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; potential role in the secretory pathway; seamless-GFP and mCherry fusion proteins localize to the vacuole, while SWAT-GFP fusion localizes to both the endoplasmic reticulum and vacuole; suppresses toxicity of slow-folding human Z-type alpha1-antitrypsin variant associated with liver cirrhosis and emphysema |
| MED6 |
YHR058C |
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress |
| FYV4 |
YHR059W |
Protein FYV4, mitochondrial; Protein of unknown function; required for survival upon exposure to K1 killer toxin; Belongs to the mitochondrion-specific ribosomal protein mS41 family |
| VMA22 |
YHR060W |
Coiled-coil domain-containing protein 115; Protein that is required for vacuolar H+-ATPase (V-ATPase) function; peripheral membrane protein; not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER) |
| GIC1 |
YHR061C |
GTPase-interacting component 1; Protein involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain; relocalizes from bud neck to nucleus upon DNA replication stress; GIC1 has a paralog, GIC2, that arose from the whole genome duplication |
| RPP1 |
YHR062C |
Ribonuclease P/MRP protein subunit RPP1; Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; relocalizes to the cytosol in response to hypoxia; Belongs to the eukaryotic/archaeal RNase P protein component 3 family |
| PAN5 |
YHR063C |
2-dehydropantoate 2-reductase PAN5; 2-dehydropantoate 2-reductase; part of the pantothenic acid pathway, structury homologous to E. coli panE |
| SSZ1 |
YHR064C |
Ribosome-associated complex subunit SSZ1; Hsp70 protein that interacts with Zuo1p (a DnaJ homolog); interacts with Zuo1p to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP |
| RRP3 |
YHR065C |
ATP-dependent rRNA helicase RRP3; Protein involved in rRNA processing; required for maturation of the 35S primary transcript of pre-rRNA and for cleavage leading to mature 18S rRNA; homologous to eIF-4a, which is a DEAD box RNA-dependent ATPase with helicase activity |
| SSF1 |
YHR066W |
Ribosome biogenesis protein SSF1; Constituent of 66S pre-ribosomal particles; required for ribosomal large subunit maturation; functiony redundant with Ssf2p; member of the Brix family; SSF1 has a paralog, SSF2, that arose from the whole genome duplication |
| HTD2 |
YHR067W |
Hydroxyacyl-thioester dehydratase type 2, mitochondrial; Mitochondrial 3-hydroxyacyl-thioester dehydratase; involved in fatty acid biosynthesis, required for respiratory growth and for normal mitochondrial morphology |
| DYS1 |
YHR068W |
Deoxyhypusine synthase; catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric; human homolog DHPS ows growth of yeast haploid dys1 null mutant after sporulation of heterozygous diploid |
| RRP4 |
YHR069C |
Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) |
| TRM5 |
YHR070W |
tRNA(m(1)G37)methyltransferase; methylates a tRNA base adjacent to the anticodon that has a role in prevention of frameshifting; localized to both cytoplasm and mitochondria, and modifies both cytoplasmic and mitochondrial tRNAs; mutations in human ortholog TRMT5 are associated with skeletal muscle respiratory chain deficiencies, and trm5 mutations analogous to disease mutations decrease respiration; Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family |
| PCL5 |
YHR071W |
PHO85 cyclin-5; Cyclin; interacts with and phosphorylated by Pho85p cyclin-dependent kinase (Cdk), induced by Gcn4p at level of transcription, specificy required for Gcn4p degradation, may be sensor of cellular protein biosynthetic capacity; Belongs to the cyclin family. PCL1,2 subfamily |
| YNCH0009C |
YNCH0009C |
Unknown |
| ERG7 |
YHR072W |
Lanosterol synthase; an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis; human LSS functiony complements the lethality of the erg7 null mutation; Belongs to the terpene cyclase/mutase family |
| NOP10 |
YHR072W-A |
H/aca ribonucleoprotein complex subunit 3; Subunit of box H/ACA snoRNP complex; required for pseudouridylation and processing of pre-18S rRNA |
| OSH3 |
YHR073W |
Member of an oxysterol-binding protein family; this family has seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability; contains FFAT motif; interacts with ER anchor Scs2p at patches at the plasma membrane; regulated by sterol binding; Belongs to the OSBP family |
| QNS1 |
YHR074W |
Nad+ synthase (glutamine-hydrolysing); Glutamine-dependent NAD(+) synthetase; essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide |
| PPE1 |
YHR075C |
Protein phosphatase methylesterase 1; Protein with carboxyl methyl esterase activity; may have a role in demethylation of the phosphoprotein phosphatase catalytic subunit; also identified as a sm subunit mitochondrial ribosomal protein; Belongs to the AB hydrolase superfamily |
| PTC7 |
YHR076W |
Type 2C serine/threonine protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope; activates coenzyme Q6 biosynthesis by dephosphorylation of demethoxy-Q6 hydroxylase Coq7p |
| NMD2 |
YHR077C |
Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance |
| YHR078W |
YHR078W |
Uncharacterized protein YHR078W; High osmolarity-regulated gene of unknown function |
| IRE1 |
YHR079C |
Serine/threonine-protein kinase/endoribonuclease IRE1; Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; role in homeostatic adaptation to ER stress; Kar2p binds inactive Ire1p and releases from it upon ER stress |
| SAE3 |
YHR079C-A |
Pachytene arrest protein SAE3; Meiosis-specific protein involved in meiotic recombination; involved in DMC1-dependent meiotic recombination; forms heterodimer with Mei5p; proposed to be an assembly factor for Dmc1p; Belongs to the SWI5/SAE3 family |
| LAM4 |
YHR080C |
Membrane-anchored lipid-binding protein LAM4; Sterol-binding protein that localizes to puncta in the cortical ER; sterol binding occurs via two StART-like domains; one of six StART-like domain-containing proteins in yeast that may be involved in intracellular sterol transfer between membranes; conserved across eukaryotes; has both GRAM and StART-like (VASt) domains; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies |
| LRP1 |
YHR081W |
Nuclear exosome-associated nucleic acid binding protein; involved in RNA processing, surveillance, degradation, tethering, and export; forms a stable heterodimer with Rrp6p and regulates its exonucleolytic activity; rapidly degraded by the proteasome in the absence of Rrp6p; homolog of mammalian nuclear matrix protein C1D involved in regulation of DNA repair and recombination |
| KSP1 |
YHR082C |
Serine/threonine-protein kinase KSP1; Serine/threonine protein kinase; associates with TORC1 and likely involved in TOR signaling cascades; negative regulator of autophagy; nuclear translocation required for haploid filamentous growth; regulates filamentous growth induced nuclear translocation of Bcy1p, Fus3p, and Sks1p; overproduction causes ele-specific suppression of prp20-10; protein abundance increases in response to DNA replication stress; Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily |
| SAM35 |
YHR083W |
Component of the sorting and assembly machinery (SAM) complex; the SAM (or TOB) complex is located in the mitochondrial outer membrane; the complex binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane |
| STE12 |
YHR084W |
Protein STE12; Transcription factor that is activated by a MAPK signaling cascade; activates genes involved in mating or pseudohyphal/invasive growth pathways; cooperates with Tec1p transcription factor to regulate genes specific for invasive growth |
| IPI1 |
YHR085W |
Pre-rRNA-processing protein IPI1; Component of the Rix1 complex and possibly pre-replicative complexes; required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-replicative complex (pre-RC) formation and maintenance during DNA replication licensing; relocalizes to the cytosol in response to hypoxia; essential gene; Belongs to the IPI1/TEX10 family |
| NAM8 |
YHR086W |
RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of REC107 pre-mRNA; Nam8p regulon embraces the meiotic pre-mRNAs of REC107, HFM1, AMA1 SPO22 and PCH2; the putative RNA binding domains RRM2 and RRM3 are required for Nam8p meiotic function |
| YHR086W-A |
YHR086W-A |
Uncharacterized protein YHR086W-A; Putative protein of unknown function; identified by fungal homology and RT-PCR; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum and vacuole respectively |
| RTC3 |
YHR087W |
Restriction of telomere capping protein 3; Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity; protein abundance increases in response to DNA replication stress |
| RPF1 |
YHR088W |
Ribosome production factor 1; Protein involved in assembly and export of the large ribosomal subunit; nucleolar protein; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA |
| GAR1 |
YHR089C |
H/ACA ribonucleoprotein complex subunit 1; Protein component of the H/ACA snoRNP pseudouridylase complex; involved in the modification and cleavage of the 18S pre-rRNA; Belongs to the GAR1 family |
| YNG2 |
YHR090C |
Chromatin modification-related protein YNG2; Subunit of NuA4, an essential histone acetyltransferase complex; positions Piccolo NuA4 for efficient acetylation of histone H4 or histone H2A; relocalizes to the cytosol in response to hypoxia; similar to human tumor suppressor ING1 and its isoforms ING4 and ING5 |
| MSR1 |
YHR091C |
Arginine--tRNA ligase, mitochondrial; Mitochondrial arginyl-tRNA synthetase; mutations in human ortholog are associated with pontocerebellar hypoplasia type 6; MSR1 has a paralog, YDR341C, that arose from the whole genome duplication; Belongs to the class-I aminoacyl-tRNA synthetase family |
| HXT4 |
YHR092C |
High-affinity glucose transporter; member of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose; HXT4 has a paralog, HXT7, that arose from the whole genome duplication |
| AHT1 |
YHR093W |
Hexose transport activator protein; Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; multicopy suppressor of glucose transport defects, likely due to the presence of an HXT4 regulatory element in the region |
| HXT1 |
YHR094C |
Mfs transporter, sp family, sugar:h+ symporter; Low-affinity glucose transporter of the major facilitator superfamily; expression is induced by Hxk2p in the presence of glucose and repressed by Rgt1p when glucose is limiting; HXT1 has a paralog, HXT6, what arose from the whole genome dupli |