Gene name: EFM5
Reference locus (S288C): YGR001C
Gene biotype: protein_coding
Description: Protein-lysine N-methyltransferase EFM5; S-adenosylmethionine-dependent lysine methyltransferase; involved in the trimethylation of eEF1A (Tef1p/Tef2p) at lysine 79; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; required for replication of Brome mosaic virus in budding yeast; expresses a circular RNA; originally misclassified as a N-6-adenine specific DNA methyltransferase based on sequence similarity; both Efm5p and human ortholog N6AMT2 can methylate eEF1a from either species in vitro
Top 10 interactors (score): EFM4 (0.981); TEF1 (0.971); EFM6 (0.956); EFM3 (0.938); HBS1 (0.911); SKI7 (0.911); NNT1 (0.887); EFM1 (0.872); HPM1 (0.862); EFM2 (0.782); RKM5 (0.777); TAD3 (0.75); YHR218W (0.708); HMRA1 (0.7); YML133C (0.693); TRM8 (0.691); YLL067C (0.67); YLL066C (0.67); SUS1 (0.658); SFM1 (0.655)
Homologues (species, bitscore): N6AMT2 (Homo sapiens, 145.6); N6amt2 (Mus musculus, 141.4); n6amt2 (Danio rerio, 125.9); n6amt2 (Xenopus tropicalis, 149.8); CG9154 (Drosophila melanogaster, 98.2); M142.8 (Caenorhabditis elegans, 115.5); AT3G58470 (Arabidopsis thaliana, 108.2)
External links:
Saccharomyces genome database (SGD)
STRING database
BioGRID
UniProt Knowledgebase
Kyoto encyclopedia of genes and genomes (KEGG)
Reactome
UCSC Genome Browser
| strain | assembly | application | macro_region | gene_coord | geneID | rnaID | protID | %id | %cov_exon | status | copyNumber | copyID | nORFs | match_ref_CDS | validORF | missing_startCodon | missing_stopCodon | outOfFrame_stopCodon |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AKU4011 | GCA_001738255.1 | Sake | Asia | CM005301.1:499186-500081:- | gene-YGR001C_AKU4011 | rna-NM_001181130.3_AKU4011 | cds-NP_011515.4_AKU4011 | 99.2 | 99.2 | Verified | 1 | 1 | 1 | False | True | False | False | False |
| BG1 | GCA_001932575.1 | Bioethanol | South America | MSHP01000060.1:76028-76924:+ | gene-YGR001C_BG1 | rna-NM_001181130.3_BG1 | cds-NP_011515.4_BG1 | 99.0 | 99.2 | Verified | 1 | 1 | 1 | False | True | False | False | False |
| Bruggeman | GCA_001738585.1 | Bread | Not Applicable | CM005509.1:500154-501050:- | gene-YGR001C_Bruggeman | rna-NM_001181130.3_Bruggeman | cds-NP_011515.4_Bruggeman | 99.0 | 99.2 | Verified | 1 | 1 | 1 | False | True | False | False | False |
| CAT1 | GCA_001738705.1 | Bioethanol | South America | CM005525.1:498948-499844:- | gene-YGR001C_CAT1 | rna-NM_001181130.3_CAT1 | cds-NP_011515.4_CAT1 | 99.0 | 99.2 | Verified | 1 | 1 | 1 | False | True | False | False | False |
| CBS1585 | GCA_001738375.1 | Sake | Asia | CM005365.1:500679-501574:- | gene-YGR001C_CBS1585 | rna-NM_001181130.3_CBS1585 | cds-NP_011515.4_CBS1585 | 99.2 | 99.2 | Verified | 1 | 1 | 1 | False | True | False | False | False |
| CBS436 | GCA_001738355.1 | Sake | Asia | CM005349.1:495041-495937:- | gene-YGR001C_CBS436 | rna-NM_001181130.3_CBS436 | cds-NP_011515.4_CBS436 | 98.8 | 99.2 | Mismatch | 1 | 1 | 1 | False | True | False | False | False |
| CBS6412 | GCA_001738345.1 | Sake | Asia | CM005333.1:493275-494170:- | gene-YGR001C_CBS6412 | rna-NM_001181130.3_CBS6412 | cds-NP_011515.4_CBS6412 | 99.2 | 99.2 | Mismatch | 1 | 1 | 1 | False | True | False | False | False |
| CBS6413 | GCA_001738265.1 | Sake | Asia | CM005317.1:497351-498246:- | gene-YGR001C_CBS6413 | rna-NM_001181130.3_CBS6413 | cds-NP_011515.4_CBS6413 | 99.0 | 99.2 | Verified | 1 | 1 | 1 | False | True | False | False | False |
| CENPK1137D | GCA_000269885.1 | Laboratory | Not Applicable | CM001528.1:496951-497852:- | gene-YGR001C_CENPK1137D | rna-NM_001181130.3_CENPK1137D | cds-NP_011515.4_CENPK1137D | 100.0 | 100.0 | Verified | 1 | 1 | 1 | True | True | False | False | False |
| EthanolRed | GCA_001738615.1 | Bioethanol | Asia | CM005477.1:487556-488452:- | gene-YGR001C_EthanolRed | rna-NM_001181130.3_EthanolRed | cds-NP_011515.4_EthanolRed | 98.8 | 99.2 | Unannotated | 1 | 1 | 0 | False | False | False | True | False |
| FaliES1 | GCA_001738715.1 | Bioethanol | Asia | CM005541.1:490807-491702:- | gene-YGR001C_FaliES1 | rna-NM_001181130.3_FaliES1 | cds-NP_011515.4_FaliES1 | 99.0 | 99.2 | Verified | 1 | 1 | 1 | False | True | False | False | False |
| JAY291 | GCA_000182315.2 | Bioethanol | South America | ACFL01000020.1:29440-30336:- | gene-YGR001C_JAY291 | rna-NM_001181130.3_JAY291 | cds-NP_011515.4_JAY291 | 99.0 | 99.2 | Verified | 1 | 1 | 1 | False | True | False | False | False |
| MUCL30387 | GCA_001738515.1 | Bread | Europe | CM005413.1:497376-498272:- | gene-YGR001C_MUCL30387 | rna-NM_001181130.3_MUCL30387 | cds-NP_011515.4_MUCL30387 | 99.0 | 99.2 | Verified | 1 | 1 | 1 | False | True | False | False | False |
| MUCL30388 | GCA_001738495.1 | Bread | Europe | CM005445.1:499927-500823:- | gene-YGR001C_MUCL30388 | rna-NM_001181130.3_MUCL30388 | cds-NP_011515.4_MUCL30388 | 98.9 | 99.2 | Verified | 1 | 1 | 1 | False | True | False | False | False |
| MUCL39482 | GCA_001738235.1 | Sake | Asia | CM005285.1:496498-497393:- | gene-YGR001C_MUCL39482 | rna-NM_001181130.3_MUCL39482 | cds-NP_011515.4_MUCL39482 | 99.2 | 99.2 | Verified | 1 | 1 | 1 | False | True | False | False | False |
| MUCL42920 | GCA_001738485.1 | Bread | Europe | CM005429.1:505927-506824:- | gene-YGR001C_MUCL42920 | rna-NM_001181130.3_MUCL42920 | cds-NP_011515.4_MUCL42920 | 99.0 | 99.2 | Verified | 1 | 1 | 1 | False | True | False | False | False |
| NCIM3186 | GCA_001029075.1 | Bioethanol | Asia | CP011816.1:497133-498034:- | gene-YGR001C_NCIM3186 | rna-NM_001181130.3_NCIM3186 | cds-NP_011515.4_NCIM3186 | 99.4 | 100.0 | Verified | 1 | 1 | 1 | False | True | False | False | False |
| NCYC1407 | GCA_001738225.1 | Sake | Asia | CM005269.1:490539-491434:- | gene-YGR001C_NCYC1407 | rna-NM_001181130.3_NCYC1407 | cds-NP_011515.4_NCYC1407 | 99.0 | 99.2 | Verified | 1 | 1 | 1 | False | True | False | False | False |
| PE2H3 | GCA_905220325.1 | Bioethanol | South America | HG994146.1:493208-494104:- | gene-YGR001C_PE2H3 | rna-NM_001181130.3_PE2H3 | cds-NP_011515.4_PE2H3 | 99.0 | 99.2 | Verified | 1 | 1 | 1 | False | True | False | False | False |
| PE2H4 | GCA_905220315.1 | Bioethanol | South America | LR999878.1:498258-499154:- | gene-YGR001C_PE2H4 | rna-NM_001181130.3_PE2H4 | cds-NP_011515.4_PE2H4 | 99.0 | 99.2 | Verified | 1 | 1 | 1 | False | True | False | False | False |
| S288C | GCF_000146045.2 | Laboratory | Not applicable | NC_001139.9:497133-498034:- | gene-YGR001C_S288C | rna-NM_001181130.3_S288C | cds-NP_011515.4_S288C | 100.0 | 100.0 | Verified | 1 | 1 | 1 | True | True | False | False | False |
| SA1 | GCA_004114265.1 | Bioethanol | South America | SDIA01000021.1:57553-58448:+ | gene-YGR001C_SA1 | rna-NM_001181130.3_SA1 | cds-NP_011515.4_SA1 | 99.2 | 99.2 | Verified | 1 | 1 | 1 | False | True | False | False | False |
| ThermosaccDry | GCA_001738605.1 | Bioethanol | Asia | CM005493.1:510133-511028:- | gene-YGR001C_ThermosaccDry | rna-NM_001181130.3_ThermosaccDry | cds-NP_011515.4_ThermosaccDry | 99.0 | 99.2 | Verified | 1 | 1 | 1 | False | True | False | False | False |
| VR1 | GCA_001738595.1 | Bioethanol | South America | CM005461.1:600419-601320:- | gene-YGR001C_VR1 | rna-NM_001181130.3_VR1 | cds-NP_011515.4_VR1 | 100.0 | 100.0 | Verified | 1 | 1 | 1 | True | True | False | False | False |
| ZTW1 | GCA_000308935.1 | Bioethanol | Asia | AMDD01000014.1:488731-489626:- | gene-YGR001C_ZTW1 | rna-NM_001181130.3_ZTW1 | cds-NP_011515.4_ZTW1 | 99.2 | 99.2 | Verified | 1 | 1 | 1 | False | True | False | False | False |
| Sparadoxus | GCF_002079055.1 | Not applicable | Not applicable | NC_047493.1:498220-499117:- | gene-YGR001C_Sparadoxus | rna-NM_001181130.3_Sparadoxus | cds-NP_011515.4_Sparadoxus | 91.1 | 100.0 | Verified | 1 | 1 | 1 | False | True | False | False | False |